Journal: Scientific Reports
Article Title: Rapid clearance of bacteria from maternal bloodstream after delivery in pregnancies complicated by preterm pre-labor rupture of the membranes
doi: 10.1038/s41598-026-37231-5
Figure Lengend Snippet: ( A ) Quantitative relationships among bacterial load in fetal membranes (FM), placental villous tissue (P) and maternal blood (MB) before and after delivery in a subset of deliveries with matched samples ( n = 27 mothers and their 31 newborns). Bacterial load among different types of samples is appreciated by dCt for bacterial 16 S RNA normalized to the human housekeeping gene glucose-6-phosphate isomerase (GPI) amplified in the same sample. ( B ) Qualitative relationships in α-diversity appreciated by the Chao-1 index of operational taxonomic units (OTU, genus level) index among FM, placental villous tissue and MB before and after delivery. Exposure status of the newborns was determined by laboratory analysis of cord blood for haptoglobin and IL-6 as described in Methods. Of the 31 newborns 17 were identified as exposed (EXP) and 14 as non-exposed (NEXP). Data is shown as mean + SEM. Groups were compared using 2-way ANOVA after log transformation; Correlation between twins was adjusted using a Generalized Estimating Equation (GEE) model. *** p < 0.001; * p < 0.05; Qualitative relationships among samples appreciated by the relative representation of bacterial families ( C ) and phyla ( D ) among sequencing reads. ( E ) Dendogram clustering of genus level operational taxonomic units (OTU) among samples from each delivery. The color intensity represents the number of reads in each sample.
Article Snippet: Glucose-6-phosphate isomerase (GPI) was amplified using TaqMan Gene Expression Assay (Hs03882242_s1, Applied Biosystems, Foster City, CA) and used as housekeeping gene based on its qualification of “exceptionally uniform gene” .
Techniques: Amplification, Transformation Assay, Sequencing
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