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cd31 polyclonal antibody, fitc conjugated  (Bioss)


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    Bioss cd31 polyclonal antibody, fitc conjugated
    Cd31 Polyclonal Antibody, Fitc Conjugated, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fitc/CD31+Polyclonal+Antibody%2C+FITC+Conjugated/custom%40bs-0468r-fitc%4042773115
    Average 90 stars, based on 5 article reviews
    cd31 polyclonal antibody, fitc conjugated - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Use of Mesenchymal Stem Cell-Targeting 3WJ Nanoparticles and Reported Specific Delivery of Anti-miRNA 138 to Treat Osteoporosis
    Article Snippet: For surface marker characteristics, after being collected and washed, MSCs were incubated with primary antibodies anti-CD29 (Bioss, bs0486R, 1:500), anti-CD44 (Bioss, bs-2507R, 1:500), and antiCD45 (Bioss, bs-0522R, 1:500) for 30 min at 4 °C. .. After being washed, MSCs were incubated with FITC-conjugated secondary antibody (Bioss, bs-0295G, 1:2000) for 30 min at 4 °C and analyzed by a flow cytometer (Beckman, USA). ..

    Article Title: Rbpms2 prevents major cardiac defects in cardiomyocyte-specific Rbpms-deficient mice.
    Article Snippet: Subsequently, cells or tissue sections were incubated with primary antibodies, RBPMS 1:1000 (Sigma; HPA056999), RBPMS2 1:300 (Abcam; ab181098), anti-alpha-Actinin 1:1000 (Sigma; A7811), CAMK2G 1:1000 (Sigma; SAB1400039), alpha-Tubulin 1:1000 (Sigma; T6074), cardiac Troponin I 1:1000 (Proteintech; 21652-1-AP), Troponin T antibody, FITC Conjugated 1:500 (Bioss; bs-2804R-FITC), Phalloidin 1:300 (Sigma; P5282), Phospho-Histone H3(Ser10) 1:1000 (Cell signaling; #9701), Ki67 1:300 (Abcam; ab15580) overnight at 4 ◦ C. The following day, cells or tissue sections were washed 3-times in 1x PBS for 5 min and then incubated for 2 hours at RT with secondary antibodies conjugated to Alexa FluorTM 488, Cy3 or Cy5, diluted in blocking solution. .. Subsequently, cells or tissue sections were incubated with primary antibodies, RBPMS 1:1000 (Sigma; HPA056999), RBPMS2 1:300 (Abcam; ab181098), anti-alpha-Actinin 1:1000 (Sigma; A7811), CAMK2G 1:1000 (Sigma; SAB1400039), alpha-Tubulin 1:1000 (Sigma; T6074), cardiac Troponin I 1:1000 (Proteintech; 21652-1-AP), Troponin T antibody, FITC Conjugated 1:500 (Bioss; bs-2804R-FITC), Phalloidin 1:300 (Sigma; P5282), Phospho-Histone H3(Ser10) 1:1000 (Cell signaling; #9701), Ki67 1:300 (Abcam; ab15580) overnight at 4 ◦ C. The following day, cells or tissue sections were washed 3-times in 1x PBS for 5 min and then incubated for 2 hours at RT with secondary antibodies conjugated to Alexa FluorTM 488, Cy3 or Cy5, diluted in blocking solution. ..

    Article Title: Modeling ANKRD26 5'-UTR mutation-related thrombocytopenia.
    Article Snippet: An anti-zebrafish Ankrd26 IgG (ABclonal; 1:300) was used for immunohistochemistry as previously described (Zheng et al., 2020). .. Additionally, human peripheral blood smears were fixed with absolute methanol and incubated with APC-conjugated anti-human CD41 antibody (Fisher Scientific; 1:200) and FITC-conjugated anti-human ANKRD26 antibody (Bioss Antibodies, Woburn, MA, USA; 1:100), followed by 4′,6-diamidino-2-phenylindole (DAPI) for nuclear staining. ..

    Article Title: Inhibition of Bif-1 confers cardio-protection in myocardial infarction.
    Article Snippet: Myocardial infarction (MI) remains a major cause of chronic heart failure.. Endoplasmic reticulum (ER) stress is an emerging therapeutic strategy to prevent adverse remodeling of the infarcted heart.. However, little is known about how Bax-interacting protein 1 (Bif-1), a member of the endophilin B family, is involved in mediating cardiac ER stress in ischemic heart disease.

    Article Title: Nano-delivery system of milk-derived exosomes loaded with Forsythiaside A: Studies on bovine mammary epithelial cells and mastitis induced mice.
    Article Snippet: Bovine mammary gland health is essential for sustaining milk yield and dairy production efficiency.. Currently, antimicrobial resistance resulting from antibiotic treatment of mastitis severely constrains the sustainable development of the dairy industry.. Forsythoside A (FTA), a natural phenylethanoid glycoside possessing potent anti-inflammatory and antioxidant activities, exhibits considerable therapeutic potential; however, its clinical translation remains hindered by poor bioavailability.

    Article Title: Use of Mesenchymal Stem Cell-Targeting 3WJ Nanoparticles and Reported Specific Delivery of Anti-miRNA 138 to Treat Osteoporosis
    Article Snippet: For surface marker characteristics, after being collected and washed, MSCs were incubated with primary antibodies anti-CD29 (Bioss, bs-0486R, 1:500), anti-CD44 (Bioss, bs-2507R, 1:500), and anti-CD45 (Bioss, bs-0522R, 1:500) for 30 min at 4 °C. .. After being washed, MSCs were incubated with FITC-conjugated secondary antibody (Bioss, bs-0295G, 1:2000) for 30 min at 4 °C and analyzed by a flow cytometer (Beckman, USA). ..

    Article Title: Optimizing carbon nanoparticle staining for sentinel lymph nodes in rabbit lower limb models: concentration and time dependence
    Article Snippet: The sections were then incubated with primary antibodies against LYVE-1 (HZ-20120R; Shanghai Huzhen Industrial Co., Ltd, Shanghai, China) and CD31 (HZ-0195R; Shanghai Huzhen Industrial Co., Ltd, Shanghai, China). .. This was followed by incubation with HRP-conjugated secondary antibodies (rabbit anti-goat HRP, bs-0296R; Bioss) and FITC-conjugated secondary antibodies (goat anti-rabbit FITC, bs-0295G; Bioss). ..

    Flow Cytometry:

    Article Title: Use of Mesenchymal Stem Cell-Targeting 3WJ Nanoparticles and Reported Specific Delivery of Anti-miRNA 138 to Treat Osteoporosis
    Article Snippet: For surface marker characteristics, after being collected and washed, MSCs were incubated with primary antibodies anti-CD29 (Bioss, bs0486R, 1:500), anti-CD44 (Bioss, bs-2507R, 1:500), and antiCD45 (Bioss, bs-0522R, 1:500) for 30 min at 4 °C. .. After being washed, MSCs were incubated with FITC-conjugated secondary antibody (Bioss, bs-0295G, 1:2000) for 30 min at 4 °C and analyzed by a flow cytometer (Beckman, USA). ..

    Article Title: Use of Mesenchymal Stem Cell-Targeting 3WJ Nanoparticles and Reported Specific Delivery of Anti-miRNA 138 to Treat Osteoporosis
    Article Snippet: For surface marker characteristics, after being collected and washed, MSCs were incubated with primary antibodies anti-CD29 (Bioss, bs-0486R, 1:500), anti-CD44 (Bioss, bs-2507R, 1:500), and anti-CD45 (Bioss, bs-0522R, 1:500) for 30 min at 4 °C. .. After being washed, MSCs were incubated with FITC-conjugated secondary antibody (Bioss, bs-0295G, 1:2000) for 30 min at 4 °C and analyzed by a flow cytometer (Beckman, USA). ..

    Blocking Assay:

    Article Title: Rbpms2 prevents major cardiac defects in cardiomyocyte-specific Rbpms-deficient mice.
    Article Snippet: Subsequently, cells or tissue sections were incubated with primary antibodies, RBPMS 1:1000 (Sigma; HPA056999), RBPMS2 1:300 (Abcam; ab181098), anti-alpha-Actinin 1:1000 (Sigma; A7811), CAMK2G 1:1000 (Sigma; SAB1400039), alpha-Tubulin 1:1000 (Sigma; T6074), cardiac Troponin I 1:1000 (Proteintech; 21652-1-AP), Troponin T antibody, FITC Conjugated 1:500 (Bioss; bs-2804R-FITC), Phalloidin 1:300 (Sigma; P5282), Phospho-Histone H3(Ser10) 1:1000 (Cell signaling; #9701), Ki67 1:300 (Abcam; ab15580) overnight at 4 ◦ C. The following day, cells or tissue sections were washed 3-times in 1x PBS for 5 min and then incubated for 2 hours at RT with secondary antibodies conjugated to Alexa FluorTM 488, Cy3 or Cy5, diluted in blocking solution. .. Subsequently, cells or tissue sections were incubated with primary antibodies, RBPMS 1:1000 (Sigma; HPA056999), RBPMS2 1:300 (Abcam; ab181098), anti-alpha-Actinin 1:1000 (Sigma; A7811), CAMK2G 1:1000 (Sigma; SAB1400039), alpha-Tubulin 1:1000 (Sigma; T6074), cardiac Troponin I 1:1000 (Proteintech; 21652-1-AP), Troponin T antibody, FITC Conjugated 1:500 (Bioss; bs-2804R-FITC), Phalloidin 1:300 (Sigma; P5282), Phospho-Histone H3(Ser10) 1:1000 (Cell signaling; #9701), Ki67 1:300 (Abcam; ab15580) overnight at 4 ◦ C. The following day, cells or tissue sections were washed 3-times in 1x PBS for 5 min and then incubated for 2 hours at RT with secondary antibodies conjugated to Alexa FluorTM 488, Cy3 or Cy5, diluted in blocking solution. ..

    Staining:

    Article Title: Modeling ANKRD26 5'-UTR mutation-related thrombocytopenia.
    Article Snippet: An anti-zebrafish Ankrd26 IgG (ABclonal; 1:300) was used for immunohistochemistry as previously described (Zheng et al., 2020). .. Additionally, human peripheral blood smears were fixed with absolute methanol and incubated with APC-conjugated anti-human CD41 antibody (Fisher Scientific; 1:200) and FITC-conjugated anti-human ANKRD26 antibody (Bioss Antibodies, Woburn, MA, USA; 1:100), followed by 4′,6-diamidino-2-phenylindole (DAPI) for nuclear staining. ..

    Labeling:

    Article Title: Nano-delivery system of milk-derived exosomes loaded with Forsythiaside A: Studies on bovine mammary epithelial cells and mastitis induced mice.
    Article Snippet: Bovine mammary gland health is essential for sustaining milk yield and dairy production efficiency.. Currently, antimicrobial resistance resulting from antibiotic treatment of mastitis severely constrains the sustainable development of the dairy industry.. Forsythoside A (FTA), a natural phenylethanoid glycoside possessing potent anti-inflammatory and antioxidant activities, exhibits considerable therapeutic potential; however, its clinical translation remains hindered by poor bioavailability.

    Fluorescence:

    Article Title: Nano-delivery system of milk-derived exosomes loaded with Forsythiaside A: Studies on bovine mammary epithelial cells and mastitis induced mice.
    Article Snippet: Bovine mammary gland health is essential for sustaining milk yield and dairy production efficiency.. Currently, antimicrobial resistance resulting from antibiotic treatment of mastitis severely constrains the sustainable development of the dairy industry.. Forsythoside A (FTA), a natural phenylethanoid glycoside possessing potent anti-inflammatory and antioxidant activities, exhibits considerable therapeutic potential; however, its clinical translation remains hindered by poor bioavailability.

    Microscopy:

    Article Title: Nano-delivery system of milk-derived exosomes loaded with Forsythiaside A: Studies on bovine mammary epithelial cells and mastitis induced mice.
    Article Snippet: Bovine mammary gland health is essential for sustaining milk yield and dairy production efficiency.. Currently, antimicrobial resistance resulting from antibiotic treatment of mastitis severely constrains the sustainable development of the dairy industry.. Forsythoside A (FTA), a natural phenylethanoid glycoside possessing potent anti-inflammatory and antioxidant activities, exhibits considerable therapeutic potential; however, its clinical translation remains hindered by poor bioavailability.



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    Image Search Results


    Relative HSP abundances in whole skeletal muscle homogenates from young adults and older adults pre and post HIT exercise. Representative Westen blots of (A) HSP72, HSP27, and αB-crystallin and (B) phosphorylated HSP27 Ser15 (pHSP27 Ser15) and pαB-crystallin Ser59 in whole muscle homogenates from the vastus lateralis of the same individuals. Calibration curves of mixed muscle homogenates are indicated and were used to determine the relative number of given proteins (see Methods). Stain-free gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Relative abundances of (C) HSP72, (D) HSP27, (E) pHSP27 Ser15, (F) αB-crystallin, and (G) pαB-crystallin Ser59 from young (circle) and older adults Pre (square) and older adults Post (triangle) HIT exercise are shown relative to average Old (pre) on a given gel (data are presented as mean ± SD). Individuals indicated by the number of symbols ( n : 5–7), with the same color assigned to the same individual and consistent across all graphs. * p ≤ 0.05 indicates Brown-Forsye and Welch’s and post hoc analysis using Games-Horwell. HIT = high-intensity training; HSP = heat shock protein; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.

    Journal: Journal of Sport and Health Science

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals

    doi: 10.1016/j.jshs.2025.101111

    Figure Lengend Snippet: Relative HSP abundances in whole skeletal muscle homogenates from young adults and older adults pre and post HIT exercise. Representative Westen blots of (A) HSP72, HSP27, and αB-crystallin and (B) phosphorylated HSP27 Ser15 (pHSP27 Ser15) and pαB-crystallin Ser59 in whole muscle homogenates from the vastus lateralis of the same individuals. Calibration curves of mixed muscle homogenates are indicated and were used to determine the relative number of given proteins (see Methods). Stain-free gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Relative abundances of (C) HSP72, (D) HSP27, (E) pHSP27 Ser15, (F) αB-crystallin, and (G) pαB-crystallin Ser59 from young (circle) and older adults Pre (square) and older adults Post (triangle) HIT exercise are shown relative to average Old (pre) on a given gel (data are presented as mean ± SD). Individuals indicated by the number of symbols ( n : 5–7), with the same color assigned to the same individual and consistent across all graphs. * p ≤ 0.05 indicates Brown-Forsye and Welch’s and post hoc analysis using Games-Horwell. HIT = high-intensity training; HSP = heat shock protein; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.

    Article Snippet: Details of antibodies used are as follows: HSP72 (1 in 500 mouse monoclonal, SMC100A; StressMarq Biosciences, Victoria, Canada); HSP27 (1 in 1000 mouse monoclonal, G3.1 ab2790; Abcam, Cambridge, UK); pHSP27 Ser15 (1 in 2000 monoclonal rabbit, ab76313; Abcam), pHSP27 Ser82 (1 in 2000 polyclonal mouse, ADI-SPA-524; Enzo Biochem, Farmingdale, NY, USA), αB-crystallin (1 in 1000 mouse monoclonal, SPA-222; StressGen Biotechnologies), pαB-crystallin Ser59 (1 in 1000 rabbit polyclonal, SPA-227; StressGen Biotechnologies).

    Techniques: Staining, Membrane

    HSP abundances in type I and II skeletal muscle fibers from young and older adults. (A, C, and F) The MHC isoform present was determined in individual muscle fiber segments from the vastus lateralis and, following pooling into type I and type II groups from a given biopsy, were analyzed by Westen blotting. Westen blots of (A) HSP72, (C) HSP27 and pHSP27 Ser15, (F) αB-crystallin and pαB-crystallin Ser59, with MHC isoforms in groups of fibers. Stain-free gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Calibration curves of mixed muscle homogenates are indicated. Relative protein abundances of (B) HSP72, (D) HSP27, (E) pHSP27 Ser15, (G) αB-crystallin, and (H) pαB-crystallin Ser59 in fibers from young (circle) and older adults (square) type I fibers (no outline) and type II fibers (outline). All fibers are expressed relative to the average older adult’s type I fibers. The same color is assigned to the same individual and is consistent with (data are presented as mean ± SD). * p < 0.05 and ** p < 0.01, mixed effect model Univariant using either Tukey’s or Games-Horwell’s multiple comparison test (see Methods). HIT = high-intensity training; HSP = heat shock protein; MHC = myosin heavy chain; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.

    Journal: Journal of Sport and Health Science

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals

    doi: 10.1016/j.jshs.2025.101111

    Figure Lengend Snippet: HSP abundances in type I and II skeletal muscle fibers from young and older adults. (A, C, and F) The MHC isoform present was determined in individual muscle fiber segments from the vastus lateralis and, following pooling into type I and type II groups from a given biopsy, were analyzed by Westen blotting. Westen blots of (A) HSP72, (C) HSP27 and pHSP27 Ser15, (F) αB-crystallin and pαB-crystallin Ser59, with MHC isoforms in groups of fibers. Stain-free gels are indicative of total protein loading, and molecular weights are indicated by markers collected under white light capture without moving the membrane between that and chemiluminescence detection. Calibration curves of mixed muscle homogenates are indicated. Relative protein abundances of (B) HSP72, (D) HSP27, (E) pHSP27 Ser15, (G) αB-crystallin, and (H) pαB-crystallin Ser59 in fibers from young (circle) and older adults (square) type I fibers (no outline) and type II fibers (outline). All fibers are expressed relative to the average older adult’s type I fibers. The same color is assigned to the same individual and is consistent with (data are presented as mean ± SD). * p < 0.05 and ** p < 0.01, mixed effect model Univariant using either Tukey’s or Games-Horwell’s multiple comparison test (see Methods). HIT = high-intensity training; HSP = heat shock protein; MHC = myosin heavy chain; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.

    Article Snippet: Details of antibodies used are as follows: HSP72 (1 in 500 mouse monoclonal, SMC100A; StressMarq Biosciences, Victoria, Canada); HSP27 (1 in 1000 mouse monoclonal, G3.1 ab2790; Abcam, Cambridge, UK); pHSP27 Ser15 (1 in 2000 monoclonal rabbit, ab76313; Abcam), pHSP27 Ser82 (1 in 2000 polyclonal mouse, ADI-SPA-524; Enzo Biochem, Farmingdale, NY, USA), αB-crystallin (1 in 1000 mouse monoclonal, SPA-222; StressGen Biotechnologies), pαB-crystallin Ser59 (1 in 1000 rabbit polyclonal, SPA-227; StressGen Biotechnologies).

    Techniques: Staining, Membrane, Comparison

    HSP abundances in type I and II skeletal fibers from older adults pre- and post HIT exercise. Relative protein abundances of (A and B) HSP72, (C and D) HSP27, (E and F) pHSP27 Ser15, (G and H) αB-crystallin, and (I and J) pαB-crystallin Ser59 in fibers from old pre and old post HIT exercise. All fibers are expressed relative to the average old pre type I fibers or relative pre type II depending on fiber type. The same color is assigned to the same Individual, consistent in both graphs and all figures. * p < 0.05 and ** p < 0.01 indicated significant difference in paired t -test (except pHSP27 Ser15 Wilcoxon match-pair rank test). Representative blots are shown in . HIT = high-intensity training; HSP = heat shock protein; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.

    Journal: Journal of Sport and Health Science

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals

    doi: 10.1016/j.jshs.2025.101111

    Figure Lengend Snippet: HSP abundances in type I and II skeletal fibers from older adults pre- and post HIT exercise. Relative protein abundances of (A and B) HSP72, (C and D) HSP27, (E and F) pHSP27 Ser15, (G and H) αB-crystallin, and (I and J) pαB-crystallin Ser59 in fibers from old pre and old post HIT exercise. All fibers are expressed relative to the average old pre type I fibers or relative pre type II depending on fiber type. The same color is assigned to the same Individual, consistent in both graphs and all figures. * p < 0.05 and ** p < 0.01 indicated significant difference in paired t -test (except pHSP27 Ser15 Wilcoxon match-pair rank test). Representative blots are shown in . HIT = high-intensity training; HSP = heat shock protein; pαB-crystallin Ser59 = phospho-αB-crystallin at Serine59; pHSP27 Ser15 = phospho-HSP27 at Serine15.

    Article Snippet: Details of antibodies used are as follows: HSP72 (1 in 500 mouse monoclonal, SMC100A; StressMarq Biosciences, Victoria, Canada); HSP27 (1 in 1000 mouse monoclonal, G3.1 ab2790; Abcam, Cambridge, UK); pHSP27 Ser15 (1 in 2000 monoclonal rabbit, ab76313; Abcam), pHSP27 Ser82 (1 in 2000 polyclonal mouse, ADI-SPA-524; Enzo Biochem, Farmingdale, NY, USA), αB-crystallin (1 in 1000 mouse monoclonal, SPA-222; StressGen Biotechnologies), pαB-crystallin Ser59 (1 in 1000 rabbit polyclonal, SPA-227; StressGen Biotechnologies).

    Techniques: