Journal: Journal of Virology

Article Title: Differential Activation of Human Monocyte-Derived and Plasmacytoid Dendritic Cells by West Nile Virus Generated in Different Host Cells

doi: 10.1128/jvi.00857-07

Figure Lengend Snippet: FIG. 4. Infection with WNV or SeV results in IRF3 translocation in mDC. Micrographs of mDC cultures treated with SeV (16 HAU/ml) (A) or WNV (MOI of 100, based on titration on Vero cells) (B) were exposed for the indicated times and then fixed and subjected to indirect immunofluorescent staining as described in Materials and Methods. Nuclei were stained with DAPI (500 ng/ml); IRF3 was detected with rabbit polyclonal anti-IRF3 decorated with an FITC label, and WNV antigen was detected with anti-WNV MHIAF decorated with a Texas Red label.

Article Snippet: After 1 h, cells were washed two times with PBS and incubated with a fluorescein isothiocyanate (FITC)-labeled goat anti-rabbit antibody (1:100) (Southern Biotechnology, Birmingham, AL) and Texas Red-labeled goat anti-mouse antibody (1:100) (KPL, Gaithersburg, MD) for the dou- ble-labeled WNV-infected samples.

Techniques: Infection, Translocation Assay, Titration, Staining

Journal: Clinical and Experimental Immunology

Article Title: Laryngeal transplantation in minipigs: early immunological outcomes

doi: 10.1111/j.1365-2249.2011.04531.x

Figure Lengend Snippet: Antibodies, isotypes and combinations used in multiple colour immunofluorescence studies of the laryngeal mucosa. Five combinations, each of three antibodies, were used

Article Snippet: Percentage areas of positive pixel staining were calculated for each fluorochrome and fluorochrome combination. table ft1 table-wrap mode="anchored" t5 caption a7 Primary antibody † to Clone Isotype Secondary antibody ‡ False colour Combination Myeloid cells CD172 742215 IgG2B IgG2b-FITC a Green 1 CD163 2A/10/11 IgG1 IgG1 - TRITC a Red 1 MHC-II MSA3 EPICS IgG2A IgG2a-AF633 b Blue 1 and 2 CD14 MIL2 IgG2B IgG2b-FITC a Green 2 CD16 c G7 IgG1 IgG1-TRITC a Red 2 Lymphoid cells CD45RC MIL15 IgM IgM-FITC a Green 3 and 4 CD25 K231.3B2 IgG1 IgG1-TRITC a Red 3 and 4 CD4 MIL17 H-M IgG2B IgG2b-AF633 b Blue 3 CD8 MIL12 IgG2A IgG2a-AF633 b Blue 4 and 5 γδ PPT 27 IgG2B IgG2b-FITC a Green 5 CD4 STH293 IgG1 IgG1-TRITC a Red 5 Open in a separate window † Mouse anti-pig; ‡ goat anti-mouse. a Southern Biotech, Birmingham, Alabama 35260, USA. b Molecular Probes, Oregon, USA. c AbD Serotec, Oxford, UK.

Techniques: Immunofluorescence