eef1ε1 (Proteintech)
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Proteintech
eef1ε1
Eef1ε1, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eef1%CE%B51/EEF1E1+Antibody/pmc08558842-21-0-2
Average 92 stars, based on 7 article reviews
Eef1ε1, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eef1%CE%B51/EEF1E1+Antibody/pmc08558842-21-0-2
Average 92 stars, based on 7 article reviews
eef1ε1 - by Bioz Stars,
2026-11
92/100 stars
Images
1) Product Images from "Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome"
Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome
Journal: Cell reports
doi: 10.1016/j.celrep.2021.109806
Figure Legend Snippet: Existing studies frame rocaglates as pure eIF4A/translation inhibitors. This study conceptually redefines rocaglates as translation remodelers that reprogram both the translation machinery, e.g., increasing eEF1ε1 translational activity, as well as the translatome, including the upregulation of numerous proteins, e.g., GEF-H1, that mediate rocaglate cytotoxicity in cancer cells. As a proof of concept, GEF-H1 activates RHOA, leading to increased JNK phosphorylation and apoptotic signaling resulting in reduced cell viability.
Techniques Used: Activity Assay, Phospho-proteomics
Figure Legend Snippet: KEY RESOURCES TABLE
Techniques Used: Plasmid Preparation, Derivative Assay, Recombinant, Protease Inhibitor, cDNA Synthesis, SYBR Green Assay, Mass Spectrometry, Construct, Software
Related Articles
Activity Assay:Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Densitometry was performed on non-saturated signals using ImageJ (NIH), and normalized to loading control. Phospho-proteomics:Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Densitometry was performed on non-saturated signals using ImageJ (NIH), and normalized to loading control. Plasmid Preparation:Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Densitometry was performed on non-saturated signals using ImageJ (NIH), and normalized to loading control. Derivative Assay:Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Densitometry was performed on non-saturated signals using ImageJ (NIH), and normalized to loading control. Recombinant:Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Densitometry was performed on non-saturated signals using ImageJ (NIH), and normalized to loading control. Protease Inhibitor:Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Densitometry was performed on non-saturated signals using ImageJ (NIH), and normalized to loading control. cDNA Synthesis:Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Densitometry was performed on non-saturated signals using ImageJ (NIH), and normalized to loading control. SYBR Green Assay:Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Densitometry was performed on non-saturated signals using ImageJ (NIH), and normalized to loading control. Mass Spectrometry:Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Densitometry was performed on non-saturated signals using ImageJ (NIH), and normalized to loading control. Construct:Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Densitometry was performed on non-saturated signals using ImageJ (NIH), and normalized to loading control. Software:Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Densitometry was performed on non-saturated signals using ImageJ (NIH), and normalized to loading control. Small Interfering RNA:Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Densitometry was performed on non-saturated signals using ImageJ (NIH), and normalized to loading control. Control:Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Densitometry was performed on non-saturated signals using ImageJ (NIH), and normalized to loading control. Transfection:Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Densitometry was performed on non-saturated signals using ImageJ (NIH), and normalized to loading control. Concentration Assay:Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome Article Snippet: Densitometry was performed on non-saturated signals using ImageJ (NIH), and normalized to loading control. |