eef1ε1 Search Results


eef1ε1  (Proteintech)
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Proteintech eef1ε1
Existing studies frame rocaglates as pure eIF4A/translation inhibitors. This study conceptually redefines rocaglates as translation remodelers that reprogram both the translation machinery, e.g., increasing <t>eEF1ε1</t> translational activity, as well as the translatome, including the upregulation of numerous proteins, e.g., GEF-H1, that mediate rocaglate cytotoxicity in cancer cells. As a proof of concept, GEF-H1 activates RHOA, leading to increased JNK phosphorylation and apoptotic signaling resulting in reduced cell viability.
Eef1ε1, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eef1%CE%B51/EEF1E1+Antibody/pmc08558842-377-89-90
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eef1ε1 - by Bioz Stars, 2026-10
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Existing studies frame rocaglates as pure eIF4A/translation inhibitors. This study conceptually redefines rocaglates as translation remodelers that reprogram both the translation machinery, e.g., increasing eEF1ε1 translational activity, as well as the translatome, including the upregulation of numerous proteins, e.g., GEF-H1, that mediate rocaglate cytotoxicity in cancer cells. As a proof of concept, GEF-H1 activates RHOA, leading to increased JNK phosphorylation and apoptotic signaling resulting in reduced cell viability.

Journal: Cell reports

Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome

doi: 10.1016/j.celrep.2021.109806

Figure Lengend Snippet: Existing studies frame rocaglates as pure eIF4A/translation inhibitors. This study conceptually redefines rocaglates as translation remodelers that reprogram both the translation machinery, e.g., increasing eEF1ε1 translational activity, as well as the translatome, including the upregulation of numerous proteins, e.g., GEF-H1, that mediate rocaglate cytotoxicity in cancer cells. As a proof of concept, GEF-H1 activates RHOA, leading to increased JNK phosphorylation and apoptotic signaling resulting in reduced cell viability.

Article Snippet: Primary antibodies: puromycin (Kerafast #Equation 0001, 1:1000); eIF4A1 (Abcam #31217, 1:1000); eIF4A2 (SCBT #sc-137148, 1:1000); CD98hc (CST #13180S, 1:500); GEF-H1 (Proteintech #24472-1-AP, 1:1000); SPEN (Novus Biologicals #NB100-58799); CEP170 (ThermoFisher Scientific #41-3200, 1:1000); DHX36 (Proteintech #13159-1-AP, 1:1000); DDX39 (Proteintech #11723-1-AP, 1:1000); JNK (CST #4672S, 1:1000); phospho-JNK (CST #4668S, 1:1000); c-JUN (CST #9165S, 1:1000); phospho-c-JUN (CST #9261S, 1:1000); RHOA (CST #2117S, 1:1000); RHO (ThermoFisher Scientific #16116, 1:1000); p130Cas (CST #13846S, 1:1000); phospho-p130Cas (CST #4011S, 1:1000); AKT (CST #4685S, 1:1000); phospho-AKT (CST #4060S, 1:1000); MCL-1 (CST #94296S, 1:1000); CPSF73 (Proteintech #11609-1-AP, 1:1000); eEF1ε1 (Proteintech #10805-1-AP, 1:1000); DDX17 (Proteintech #19910-1-AP); PABPN1 (Proteintech #66807-1-Ig); Histone H3 (CST #4499S, 1:2000); GAPDH (CST #5174T, 1:2000); CYPB (ThermoFisher Scientific #PA1-027A, 1:10000).

Techniques: Activity Assay, Phospho-proteomics

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome

doi: 10.1016/j.celrep.2021.109806

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Primary antibodies: puromycin (Kerafast #Equation 0001, 1:1000); eIF4A1 (Abcam #31217, 1:1000); eIF4A2 (SCBT #sc-137148, 1:1000); CD98hc (CST #13180S, 1:500); GEF-H1 (Proteintech #24472-1-AP, 1:1000); SPEN (Novus Biologicals #NB100-58799); CEP170 (ThermoFisher Scientific #41-3200, 1:1000); DHX36 (Proteintech #13159-1-AP, 1:1000); DDX39 (Proteintech #11723-1-AP, 1:1000); JNK (CST #4672S, 1:1000); phospho-JNK (CST #4668S, 1:1000); c-JUN (CST #9165S, 1:1000); phospho-c-JUN (CST #9261S, 1:1000); RHOA (CST #2117S, 1:1000); RHO (ThermoFisher Scientific #16116, 1:1000); p130Cas (CST #13846S, 1:1000); phospho-p130Cas (CST #4011S, 1:1000); AKT (CST #4685S, 1:1000); phospho-AKT (CST #4060S, 1:1000); MCL-1 (CST #94296S, 1:1000); CPSF73 (Proteintech #11609-1-AP, 1:1000); eEF1ε1 (Proteintech #10805-1-AP, 1:1000); DDX17 (Proteintech #19910-1-AP); PABPN1 (Proteintech #66807-1-Ig); Histone H3 (CST #4499S, 1:2000); GAPDH (CST #5174T, 1:2000); CYPB (ThermoFisher Scientific #PA1-027A, 1:10000).

Techniques: Plasmid Preparation, Derivative Assay, Recombinant, Protease Inhibitor, cDNA Synthesis, SYBR Green Assay, Mass Spectrometry, Construct, Software