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dapi dye  (Beyotime)


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    Structured Review

    Beyotime dapi dye
    Dapi Dye, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 31805 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dye+dapi/DAPI/pmc13123509-556-26-32
    Average 99 stars, based on 31805 article reviews
    dapi dye - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: A SARS-CoV-2 oral vaccine development strategy based on the attenuated Salmonella type III secretion system.
    Article Snippet: After three washes with PBST, monkey anti- rabbit IgG fluorescent secondary antibody (Absin, abs20021, China) and Salmonella fluorescent antibody (Jiangsu TargetPharma Laboratories, Inc.) were incubated at room temperature for 1 h in the dark. .. After three washes with PBST, the nuclear dye DAPI (Beyotime, C1002, China) was added, and then, a fluorescence microscope (Carl Zeiss Axioplan 2, Germany) was used to observe and photograph the cells. ..

    Article Title: A SARS‐C o V ‐2 oral vaccine development strategy based on the attenuated Salmonella type III secretion system
    Article Snippet: After three washes with PBST, monkey anti‐rabbit IgG fluorescent secondary antibody (Absin, abs20021, China) and Salmonella fluorescent antibody (Jiangsu TargetPharma Laboratories, Inc.) were incubated at room temperature for 1 h in the dark. .. After three washes with PBST, the nuclear dye DAPI (Beyotime, C1002, China) was added, and then, a fluorescence microscope (Carl Zeiss Axioplan 2, Germany) was used to observe and photograph the cells. ..

    Microscopy:

    Article Title: A SARS-CoV-2 oral vaccine development strategy based on the attenuated Salmonella type III secretion system.
    Article Snippet: After three washes with PBST, monkey anti- rabbit IgG fluorescent secondary antibody (Absin, abs20021, China) and Salmonella fluorescent antibody (Jiangsu TargetPharma Laboratories, Inc.) were incubated at room temperature for 1 h in the dark. .. After three washes with PBST, the nuclear dye DAPI (Beyotime, C1002, China) was added, and then, a fluorescence microscope (Carl Zeiss Axioplan 2, Germany) was used to observe and photograph the cells. ..

    Article Title: A SARS‐C o V ‐2 oral vaccine development strategy based on the attenuated Salmonella type III secretion system
    Article Snippet: After three washes with PBST, monkey anti‐rabbit IgG fluorescent secondary antibody (Absin, abs20021, China) and Salmonella fluorescent antibody (Jiangsu TargetPharma Laboratories, Inc.) were incubated at room temperature for 1 h in the dark. .. After three washes with PBST, the nuclear dye DAPI (Beyotime, C1002, China) was added, and then, a fluorescence microscope (Carl Zeiss Axioplan 2, Germany) was used to observe and photograph the cells. ..

    Incubation:

    Article Title: Natural product library screening identifies Darutigenol for the treatment of myocardial infarction and ischemia/reperfusion injury.
    Article Snippet: Primal antibodies of Ki67 (Servicebio, GB111499, 1:500) and pH3 (CST, 9701S, 1:300) were then co-incubated with cTnT antibody (Proteintech, 15513-1- AP, 1:300) to the CMs, respectively, following blocking with 5% BSA for one hour at room temperature. .. Next, CMs were rinsed three times with PBS and incubated with nucleic dye DAPI (Beyotime, C1006) for 15 min. .. Finally, the CMs were incubated with CoraLite488-conjugated Goat Anti-Mouse IgG (Proteintech, SA00013-1, 1:300) and Cy3-conjugated Goat Anti-Rabbit IgG (Proteintech, SA00009-2, 1:300) for one hour at room temperature and then washed three times with PBS.

    Article Title: Natural product library screening identifies Darutigenol for the treatment of myocardial infarction and ischemia/reperfusion injury
    Article Snippet: Primal antibodies of Ki67 (Servicebio, GB111499 , 1:500) and pH3 (CST, 9701S, 1:300) were then co-incubated with cTnT antibody (Proteintech, 15513-1-AP, 1:300) to the CMs, respectively, following blocking with 5% BSA for one hour at room temperature. .. Next, CMs were rinsed three times with PBS and incubated with nucleic dye DAPI (Beyotime, C1006) for 15 min. .. Finally, the CMs were incubated with CoraLite488-conjugated Goat Anti-Mouse IgG (Proteintech, SA00013-1, 1:300) and Cy3-conjugated Goat Anti-Rabbit IgG (Proteintech, SA00009-2, 1:300) for one hour at room temperature and then washed three times with PBS.

    Article Title: Kongensin A targeting PI3K attenuates inflammation-induced osteoarthritis by modulating macrophage polarization and alleviating inflammatory signaling.
    Article Snippet: The inflammatory microenvironment, polarization of macrophages towards the M1 phenotype, and consequent matrix degradation and senescence of chondrocytes are primary contributors to the degeneration of knee joint cartilage, further exacerbating the progression of osteoarthritis (OA).. Kongensin A (KA) is a recently identified natural plant extract exhibiting anti-necrotic apoptosis and anti-inflammatory properties, but the potential efficacy in alleviating OA remains uncertain.. The current research lucubrated the effect of KA on the inflammatory microenvironment and macrophage polarization, as well as its regulatory function in extracellular matrix (ECM) metabolism and chondrocyte senescence.

    Staining:

    Article Title: Kongensin A targeting PI3K attenuates inflammation-induced osteoarthritis by modulating macrophage polarization and alleviating inflammatory signaling.
    Article Snippet: The inflammatory microenvironment, polarization of macrophages towards the M1 phenotype, and consequent matrix degradation and senescence of chondrocytes are primary contributors to the degeneration of knee joint cartilage, further exacerbating the progression of osteoarthritis (OA).. Kongensin A (KA) is a recently identified natural plant extract exhibiting anti-necrotic apoptosis and anti-inflammatory properties, but the potential efficacy in alleviating OA remains uncertain.. The current research lucubrated the effect of KA on the inflammatory microenvironment and macrophage polarization, as well as its regulatory function in extracellular matrix (ECM) metabolism and chondrocyte senescence.



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    Image Search Results


    ( A ) Graphs depicting main cluster interactors based on incoming and outgoing interaction strengths in the tumor edge (left) and in the tumor core (right). ( B ) Representative immunofluorescent staining (left) and corresponding spatial plots (right) of CD8 + T cells (CD8 + ), monocytes (Mono, CD14 + IBA1 − ), and MoMs (CD14 + IBA1 + ) in the tumor edge and tumor core. Scale bars, 50 μm. ( C ) SpatialScore calculated from immunofluorescent data per patient in tumor edge and tumor core. Error bars denotes means ± SEM. P values, Wilcoxon rank-sum test. ( D ) Heatmap depicting selected ligand-receptor interactions from T cell clusters to THBS1 + monocytes (top) and LR interactions from THBS1 + monocytes to T cell clusters (bottom), enriched in the tumor edge. ( E ) Heatmap depicting selected LR interactions from THBS1 + monocytes to T cell clusters, enriched in the tumor edge. ( F ) Scatter plot shows the correlation between the abundance of SPP1 + macrophages and exhausted CD8 + T (CD8 + Tex) cells in the CRLM microarray dataset GSE159216 ( n = 171). The error band indicates the 95% confidence interval. In (D) and (E), color intensity represents the probability of communication. DAPI, 4′,6-diamidino-2-phenylindole.

    Journal: Science Advances

    Article Title: Multiregional profiling reveals THBS1 - SPP1 monocyte-macrophage axis drives immunosuppression and outcome in colorectal liver metastases

    doi: 10.1126/sciadv.aed1296

    Figure Lengend Snippet: ( A ) Graphs depicting main cluster interactors based on incoming and outgoing interaction strengths in the tumor edge (left) and in the tumor core (right). ( B ) Representative immunofluorescent staining (left) and corresponding spatial plots (right) of CD8 + T cells (CD8 + ), monocytes (Mono, CD14 + IBA1 − ), and MoMs (CD14 + IBA1 + ) in the tumor edge and tumor core. Scale bars, 50 μm. ( C ) SpatialScore calculated from immunofluorescent data per patient in tumor edge and tumor core. Error bars denotes means ± SEM. P values, Wilcoxon rank-sum test. ( D ) Heatmap depicting selected ligand-receptor interactions from T cell clusters to THBS1 + monocytes (top) and LR interactions from THBS1 + monocytes to T cell clusters (bottom), enriched in the tumor edge. ( E ) Heatmap depicting selected LR interactions from THBS1 + monocytes to T cell clusters, enriched in the tumor edge. ( F ) Scatter plot shows the correlation between the abundance of SPP1 + macrophages and exhausted CD8 + T (CD8 + Tex) cells in the CRLM microarray dataset GSE159216 ( n = 171). The error band indicates the 95% confidence interval. In (D) and (E), color intensity represents the probability of communication. DAPI, 4′,6-diamidino-2-phenylindole.

    Article Snippet: Sections were then incubated with primary antibodies at 4°C overnight followed by incubation with fluorophore-conjugated secondary antibodies (table S11) and nuclear dye 4′,6-diamidino-2-phenylindole (Thermo Fisher Scientific) at room temperature for 2 hours.

    Techniques: Staining, Microarray