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dyes 4  (MedChemExpress)


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    Structured Review

    MedChemExpress dyes 4
    Dyes 4, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 198 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dye+dapi/DAPI/pm42430828-62-2-14
    Average 98 stars, based on 198 article reviews
    dyes 4 - by Bioz Stars, 2026-11
    98/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Lysophospholipase PNPLA7 is a critical checkpoint of phosphatidylcholine catabolism and turnover for skeletal muscle homeostasis.
    Article Snippet: .. After fixation with 4% PFA, the cells were stained with BODIPY 493/503 (MedChemExpress, #HY-W090090) and DAPI. ..

    Article Title: BMAL1 deficiency exacerbates HDM-induced asthma by promoting JUN-mediated lipid peroxidation and airway epithelial ferroptosis.
    Article Snippet: .. Paraffin sections of lung tissue (4 μm) were incubated overnight at 4 ◦C with the following primary antibodies: COX2 (1:200, 27308-1-AP, Proteintech), JUN (1:200, YM8321, Immunoway) and stained with Alexa Fluor 594 Donkey anti-Rabbit IgG (H + L) secondary antibody (A21207, Invitrogen) at room temperature for 1 h, after which nuclei were counterstained with DAPI (MedChemExpress). ..

    Article Title: Targeting E-Selectin and ICAM1 Suppresses Endometriosis Progression by Disrupting the Inflammation–Fibrosis Axis and Reducing Local M2 Macrophage Infiltration
    Article Snippet: After that, HRP-conjugated secondary antibody and Cy5-Tyramide (MCE, Cat. No. HY-136247) were added for incubation. .. Finally, the cell nuclei were stained with DAPI (MCE, Cat. No. HY-D2868), the sections were mounted with an anti-fluorescence quenching mounting medium (MCE, Cat. No. HY-K1042), and the stained sections were observed under an Olympus BX63 automated fluorescence microscope (Olympus, Japan) with a 40 × objective lens. ..

    Incubation:

    Article Title: BMAL1 deficiency exacerbates HDM-induced asthma by promoting JUN-mediated lipid peroxidation and airway epithelial ferroptosis.
    Article Snippet: .. Paraffin sections of lung tissue (4 μm) were incubated overnight at 4 ◦C with the following primary antibodies: COX2 (1:200, 27308-1-AP, Proteintech), JUN (1:200, YM8321, Immunoway) and stained with Alexa Fluor 594 Donkey anti-Rabbit IgG (H + L) secondary antibody (A21207, Invitrogen) at room temperature for 1 h, after which nuclei were counterstained with DAPI (MedChemExpress). ..

    other:

    Article Title: Sirtuin 2 Contributes to Pancreatic Injury and Inflammation by Modulating Immediate Early Response 3–Mediated Ferroptosis in Acute Pancreatitis
    Article Snippet: Nrf2 positively regulates SLC7A11, whereas IER3 negatively regulates SLC7A11 by suppressing Nrf2 activation in AP. ( A and B ) Representative immunofluorescence images and relative fluorescence intensity of p-Nrf2 ( green ) costained with 4′,6-diamidino-2-phenylindole (DAPI; blue ) in AR42J cells treated with phosphate-buffered saline (PBS; Con), si-IER3, ML385 (HY-100523, MCE), si-IER3 + ML385, caerulein (AP), si-IER3 + AP, ML385 + AP, si-IER3 + ML385 + AP. ( C ) Dual-luciferase reporter assay of SLC7A11 promoter activity in AR42J cells treated with PBS (Con), si-IER3, ML385, si-IER3 + ML385, caerulein (AP), si-IER3 + AP, ML385 + AP, si-IER3 + ML385 + AP. ( D–F ) Representative Western blot images, relative protein and mRNA levels of SLC7A11 in AR42J cells treated with PBS (Con), si-IER3, ML385, si-IER3 + ML385, caerulein (AP), si-IER3 + AP, ML385 + AP, si-IER3 + ML385 + AP. ( G and H ) Representative immunofluorescence images and relative fluorescence intensity of p-Nrf2 ( green ) costained with DAPI ( blue ) in AR42J cells treated with PBS (Con), pCMV-HA-IER3, TBHQ (HY-100489, MCE), pCMV-HA-IER3 + TBHQ, caerulein (AP), pCMV-HA-IER3 + AP, TBHQ + AP, pCMV-HA-IER3 + TBHQ + AP. ( I ) Dual-luciferase reporter assay of SLC7A11 promoter activity in AR42J cells treated with PBS (Con), pCMV-HA-IER3, TBHQ, pCMV-HA-IER3 + TBHQ, caerulein (AP), pCMV-HA-IER3 + AP, TBHQ + AP, pCMV-HA-IER3 + TBHQ + AP. ( J–L ) Representative Western blot images, relative protein and mRNA levels of SLC7A11 in AR42J cells treated with PBS (Con), pCMV-HA-IER3, TBHQ, pCMV-HA-IER3 + TBHQ, caerulein (AP), pCMV-HA-IER3 + AP, TBHQ + AP, pCMV-HA-IER3 + TBHQ + AP.

    Article Title: Enrichment of Akkermansia muciniphila by red ginseng promotes GDF15 secretion and suppresses obesity in mice.
    Article Snippet: Background: Obesity is a growing global health burden with rising incidence.. Red ginseng (RGS), a traditional processed ginseng product, shows potential for improving metabolic parameters, though its anti-obesity mechanism remains incompletely understood.. Purpose: This study investigated the therapeutic effects of short-term RGS administration on obesity and sought to elucidate the underlying mechanism.

    Automated:

    Article Title: Targeting E-Selectin and ICAM1 Suppresses Endometriosis Progression by Disrupting the Inflammation–Fibrosis Axis and Reducing Local M2 Macrophage Infiltration
    Article Snippet: After that, HRP-conjugated secondary antibody and Cy5-Tyramide (MCE, Cat. No. HY-136247) were added for incubation. .. Finally, the cell nuclei were stained with DAPI (MCE, Cat. No. HY-D2868), the sections were mounted with an anti-fluorescence quenching mounting medium (MCE, Cat. No. HY-K1042), and the stained sections were observed under an Olympus BX63 automated fluorescence microscope (Olympus, Japan) with a 40 × objective lens. ..

    Fluorescence:

    Article Title: Targeting E-Selectin and ICAM1 Suppresses Endometriosis Progression by Disrupting the Inflammation–Fibrosis Axis and Reducing Local M2 Macrophage Infiltration
    Article Snippet: After that, HRP-conjugated secondary antibody and Cy5-Tyramide (MCE, Cat. No. HY-136247) were added for incubation. .. Finally, the cell nuclei were stained with DAPI (MCE, Cat. No. HY-D2868), the sections were mounted with an anti-fluorescence quenching mounting medium (MCE, Cat. No. HY-K1042), and the stained sections were observed under an Olympus BX63 automated fluorescence microscope (Olympus, Japan) with a 40 × objective lens. ..

    Article Title: Branched-chain amino acid depletion and adolescent myopia progression through mTORC1-dependent scleral remodeling
    Article Snippet: For immunofluorescence, cells were fixed, stained with anti-α-SMA primary 710 antibody overnight at 4 °C, followed by incubation with CoraLite488-conjugated secondary 711 Jo u n al Pr e-p roo f 24 antibody (Proteintech, Hubei, China; Cat#SA00013-2) for 1 h at room temperature in the dark. .. 712 The nuclei were counterstained with DAPI (MedChemExpress, New Jersey, USA; Cat#HY-713 D1738) for 5 min at room temperature in the dark, and fluorescence images were captured using 714 a confocal laser scanning microscope (FV4000, Olympus, Tokyo, Japan). .. Serum amino acids 715 and TGF-β were measured by ELISA (MyBioSource, California, USA; Cat#MBS2612526, 716 Cat#MBS7276965, Cat#MBS288119, Cat#MBS2701292) according to the manufacturer’s 717 instructions.

    Microscopy:

    Article Title: Targeting E-Selectin and ICAM1 Suppresses Endometriosis Progression by Disrupting the Inflammation–Fibrosis Axis and Reducing Local M2 Macrophage Infiltration
    Article Snippet: After that, HRP-conjugated secondary antibody and Cy5-Tyramide (MCE, Cat. No. HY-136247) were added for incubation. .. Finally, the cell nuclei were stained with DAPI (MCE, Cat. No. HY-D2868), the sections were mounted with an anti-fluorescence quenching mounting medium (MCE, Cat. No. HY-K1042), and the stained sections were observed under an Olympus BX63 automated fluorescence microscope (Olympus, Japan) with a 40 × objective lens. ..

    Confocal:

    Article Title: Branched-chain amino acid depletion and adolescent myopia progression through mTORC1-dependent scleral remodeling
    Article Snippet: For immunofluorescence, cells were fixed, stained with anti-α-SMA primary 710 antibody overnight at 4 °C, followed by incubation with CoraLite488-conjugated secondary 711 Jo u n al Pr e-p roo f 24 antibody (Proteintech, Hubei, China; Cat#SA00013-2) for 1 h at room temperature in the dark. .. 712 The nuclei were counterstained with DAPI (MedChemExpress, New Jersey, USA; Cat#HY-713 D1738) for 5 min at room temperature in the dark, and fluorescence images were captured using 714 a confocal laser scanning microscope (FV4000, Olympus, Tokyo, Japan). .. Serum amino acids 715 and TGF-β were measured by ELISA (MyBioSource, California, USA; Cat#MBS2612526, 716 Cat#MBS7276965, Cat#MBS288119, Cat#MBS2701292) according to the manufacturer’s 717 instructions.

    Laser-Scanning Microscopy:

    Article Title: Branched-chain amino acid depletion and adolescent myopia progression through mTORC1-dependent scleral remodeling
    Article Snippet: For immunofluorescence, cells were fixed, stained with anti-α-SMA primary 710 antibody overnight at 4 °C, followed by incubation with CoraLite488-conjugated secondary 711 Jo u n al Pr e-p roo f 24 antibody (Proteintech, Hubei, China; Cat#SA00013-2) for 1 h at room temperature in the dark. .. 712 The nuclei were counterstained with DAPI (MedChemExpress, New Jersey, USA; Cat#HY-713 D1738) for 5 min at room temperature in the dark, and fluorescence images were captured using 714 a confocal laser scanning microscope (FV4000, Olympus, Tokyo, Japan). .. Serum amino acids 715 and TGF-β were measured by ELISA (MyBioSource, California, USA; Cat#MBS2612526, 716 Cat#MBS7276965, Cat#MBS288119, Cat#MBS2701292) according to the manufacturer’s 717 instructions.



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    Image Search Results


    ( A ) Graphs depicting main cluster interactors based on incoming and outgoing interaction strengths in the tumor edge (left) and in the tumor core (right). ( B ) Representative immunofluorescent staining (left) and corresponding spatial plots (right) of CD8 + T cells (CD8 + ), monocytes (Mono, CD14 + IBA1 − ), and MoMs (CD14 + IBA1 + ) in the tumor edge and tumor core. Scale bars, 50 μm. ( C ) SpatialScore calculated from immunofluorescent data per patient in tumor edge and tumor core. Error bars denotes means ± SEM. P values, Wilcoxon rank-sum test. ( D ) Heatmap depicting selected ligand-receptor interactions from T cell clusters to THBS1 + monocytes (top) and LR interactions from THBS1 + monocytes to T cell clusters (bottom), enriched in the tumor edge. ( E ) Heatmap depicting selected LR interactions from THBS1 + monocytes to T cell clusters, enriched in the tumor edge. ( F ) Scatter plot shows the correlation between the abundance of SPP1 + macrophages and exhausted CD8 + T (CD8 + Tex) cells in the CRLM microarray dataset GSE159216 ( n = 171). The error band indicates the 95% confidence interval. In (D) and (E), color intensity represents the probability of communication. DAPI, 4′,6-diamidino-2-phenylindole.

    Journal: Science Advances

    Article Title: Multiregional profiling reveals THBS1 - SPP1 monocyte-macrophage axis drives immunosuppression and outcome in colorectal liver metastases

    doi: 10.1126/sciadv.aed1296

    Figure Lengend Snippet: ( A ) Graphs depicting main cluster interactors based on incoming and outgoing interaction strengths in the tumor edge (left) and in the tumor core (right). ( B ) Representative immunofluorescent staining (left) and corresponding spatial plots (right) of CD8 + T cells (CD8 + ), monocytes (Mono, CD14 + IBA1 − ), and MoMs (CD14 + IBA1 + ) in the tumor edge and tumor core. Scale bars, 50 μm. ( C ) SpatialScore calculated from immunofluorescent data per patient in tumor edge and tumor core. Error bars denotes means ± SEM. P values, Wilcoxon rank-sum test. ( D ) Heatmap depicting selected ligand-receptor interactions from T cell clusters to THBS1 + monocytes (top) and LR interactions from THBS1 + monocytes to T cell clusters (bottom), enriched in the tumor edge. ( E ) Heatmap depicting selected LR interactions from THBS1 + monocytes to T cell clusters, enriched in the tumor edge. ( F ) Scatter plot shows the correlation between the abundance of SPP1 + macrophages and exhausted CD8 + T (CD8 + Tex) cells in the CRLM microarray dataset GSE159216 ( n = 171). The error band indicates the 95% confidence interval. In (D) and (E), color intensity represents the probability of communication. DAPI, 4′,6-diamidino-2-phenylindole.

    Article Snippet: Sections were then incubated with primary antibodies at 4°C overnight followed by incubation with fluorophore-conjugated secondary antibodies (table S11) and nuclear dye 4′,6-diamidino-2-phenylindole (Thermo Fisher Scientific) at room temperature for 2 hours.

    Techniques: Staining, Microarray