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dapi dye  (Beyotime)


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    Structured Review

    Beyotime dapi dye
    Dapi Dye, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 33153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dye+dapi/DAPI/pmc13123509-556-26-32
    Average 99 stars, based on 33153 article reviews
    dapi dye - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Staining:

    Article Title: SATB1 lowers the T cell receptor signaling threshold for thymocyte positive selection via super-enhancer reprogramming.
    Article Snippet: Followed by 3 × PBS wash, followed by incubation with a secondary antibody in conjunctionwithAlexa 647fluorescence (FD0144) 1:1000 in 1 × PBS for onehour in the dark. .. Afterwashing three times, the nuclei were stained with 10 μg/ml DAPI (Beyotime). .. Images were acquired by a confocal microscope (Carl Zeiss, LSM 880 with Airyscan or Nikon A1 R) with a 100x objective.

    Article Title: CD147 drives keloid formation by promoting fibroblast proliferation via the CENPF signaling pathway.
    Article Snippet: After being inoculated onto 24-well culture plates and subjected to the indicated treatments, the CoraLite488 TUNEL assay apoptosis detection kit (Proteintech, Wuhan, China) was used to detect apoptotic cells according to the manufacturer’s instructions. .. Nuclei were stained with 4′,6- diamidino-2-phenylindole (DAPI, Beyotime, Shanghai, China) for 5 min. TUNEL-positive apoptotic cells were labeled with green fluorescence and visualized by a Leica Microsystems microscope (Solms, Germany). ..

    Article Title: The AGEs/RAGE Axis Contributes to Myocardial Cell Death in Type 1 Diabetic Cardiomyopathy via the ROS/NF‐ κ B/NLRP3 Inflammasome Pathway
    Article Snippet: β ‐Actin (Cat#R1207‐1) was purchased from Huabio (Harbin, China). .. Trypsin solution (Cat#C0201), CM‐H2DCFDA staining (Cat#S0035S), Cy3‐conjugated secondary antibody (Cat# A0521), FITC‐conjugated secondary antibody (Cat# A0568), 4 ′ ,6‐diamidino‐2‐phenylindole (DAPI) (CatC1005), RIPA (Cat#P0013B), protein SDS‐PAGE protein loading buffer (5X) (Cat#P0015), BCA protein concentration measurement kit (Cat#P0011), and lactate dehydrogenase (LDH) cytotoxicity assay kit (Cat#C0016) were purchased from Beyotime (Beijing, China). .. α ‐Actinin (Cat#GTX11007) was from GeneTex (Texas, United States).

    Blocking Assay:

    Article Title: NSUN3‐Associated m5C Modification of tRF5‐GlyTCC Suppresses Colorectal Cancer Progression via RPL23‐Linked Nucleolar Stress and p53‐Associated Autophagy and Enhances 5‐FU Sensitivity
    Article Snippet: .. Cells were fixed with 4% paraformaldehyde for 15 min and permeabilized with 0.1% Triton X‐100 for 10 min. After blocking with 5% BSA for 1 h, cells were incubated with an anti‐NPM1 antibody (Abcam, UK) overnight at 4°C, followed by incubation with an Alexa Fluor‐conjugated secondary antibody for 1 h. Nuclei were counterstained with DAPI (Beyotime, China). ..

    Incubation:

    Article Title: NSUN3‐Associated m5C Modification of tRF5‐GlyTCC Suppresses Colorectal Cancer Progression via RPL23‐Linked Nucleolar Stress and p53‐Associated Autophagy and Enhances 5‐FU Sensitivity
    Article Snippet: .. Cells were fixed with 4% paraformaldehyde for 15 min and permeabilized with 0.1% Triton X‐100 for 10 min. After blocking with 5% BSA for 1 h, cells were incubated with an anti‐NPM1 antibody (Abcam, UK) overnight at 4°C, followed by incubation with an Alexa Fluor‐conjugated secondary antibody for 1 h. Nuclei were counterstained with DAPI (Beyotime, China). ..

    Article Title: Lineage-specific and gestational age–dependent DNA methylation programming orchestrates human placental development
    Article Snippet: Then, they were incubated with 5mC (61255, Active Motif), EGFR (ab30, Abcam), HLA-G (ab52454, Abcam), TFAP2C (sc12762, Santa Cruz Biotechnology), CDH2 (ab18203, Abcam), EpCAM (MA5-12436, Thermo Fisher Scientific), TP63 (ab124762, Abcam), SDC1 (67155-1-Ig, Proteintech), CGB (ab9582, Abcam), or α-SMA (MA1-06110, Thermo Fisher Scientific) overnight at 4°C. .. After multiple washes to remove residual primary antibody, cells or sections were then incubated with AF488/AF594-conjugated secondary antibodies (Thermo Fisher Scientific) for 1 hour at room temperature and mounted in prolonged anti-fade reagent with 4′,6-diamidino-2-phenylindole (DAPI, Beyotime). .. Confocal imaging was performed with Zeiss LSM 880 confocal microscopes or LEICA TCS SP8, and analyzed with Zeiss Zen blue edition.

    TUNEL Assay:

    Article Title: CD147 drives keloid formation by promoting fibroblast proliferation via the CENPF signaling pathway.
    Article Snippet: After being inoculated onto 24-well culture plates and subjected to the indicated treatments, the CoraLite488 TUNEL assay apoptosis detection kit (Proteintech, Wuhan, China) was used to detect apoptotic cells according to the manufacturer’s instructions. .. Nuclei were stained with 4′,6- diamidino-2-phenylindole (DAPI, Beyotime, Shanghai, China) for 5 min. TUNEL-positive apoptotic cells were labeled with green fluorescence and visualized by a Leica Microsystems microscope (Solms, Germany). ..

    Labeling:

    Article Title: CD147 drives keloid formation by promoting fibroblast proliferation via the CENPF signaling pathway.
    Article Snippet: After being inoculated onto 24-well culture plates and subjected to the indicated treatments, the CoraLite488 TUNEL assay apoptosis detection kit (Proteintech, Wuhan, China) was used to detect apoptotic cells according to the manufacturer’s instructions. .. Nuclei were stained with 4′,6- diamidino-2-phenylindole (DAPI, Beyotime, Shanghai, China) for 5 min. TUNEL-positive apoptotic cells were labeled with green fluorescence and visualized by a Leica Microsystems microscope (Solms, Germany). ..

    Fluorescence:

    Article Title: CD147 drives keloid formation by promoting fibroblast proliferation via the CENPF signaling pathway.
    Article Snippet: After being inoculated onto 24-well culture plates and subjected to the indicated treatments, the CoraLite488 TUNEL assay apoptosis detection kit (Proteintech, Wuhan, China) was used to detect apoptotic cells according to the manufacturer’s instructions. .. Nuclei were stained with 4′,6- diamidino-2-phenylindole (DAPI, Beyotime, Shanghai, China) for 5 min. TUNEL-positive apoptotic cells were labeled with green fluorescence and visualized by a Leica Microsystems microscope (Solms, Germany). ..

    Microscopy:

    Article Title: CD147 drives keloid formation by promoting fibroblast proliferation via the CENPF signaling pathway.
    Article Snippet: After being inoculated onto 24-well culture plates and subjected to the indicated treatments, the CoraLite488 TUNEL assay apoptosis detection kit (Proteintech, Wuhan, China) was used to detect apoptotic cells according to the manufacturer’s instructions. .. Nuclei were stained with 4′,6- diamidino-2-phenylindole (DAPI, Beyotime, Shanghai, China) for 5 min. TUNEL-positive apoptotic cells were labeled with green fluorescence and visualized by a Leica Microsystems microscope (Solms, Germany). ..

    BIA-KA:

    Article Title: The AGEs/RAGE Axis Contributes to Myocardial Cell Death in Type 1 Diabetic Cardiomyopathy via the ROS/NF‐ κ B/NLRP3 Inflammasome Pathway
    Article Snippet: β ‐Actin (Cat#R1207‐1) was purchased from Huabio (Harbin, China). .. Trypsin solution (Cat#C0201), CM‐H2DCFDA staining (Cat#S0035S), Cy3‐conjugated secondary antibody (Cat# A0521), FITC‐conjugated secondary antibody (Cat# A0568), 4 ′ ,6‐diamidino‐2‐phenylindole (DAPI) (CatC1005), RIPA (Cat#P0013B), protein SDS‐PAGE protein loading buffer (5X) (Cat#P0015), BCA protein concentration measurement kit (Cat#P0011), and lactate dehydrogenase (LDH) cytotoxicity assay kit (Cat#C0016) were purchased from Beyotime (Beijing, China). .. α ‐Actinin (Cat#GTX11007) was from GeneTex (Texas, United States).

    Protein Concentration:

    Article Title: The AGEs/RAGE Axis Contributes to Myocardial Cell Death in Type 1 Diabetic Cardiomyopathy via the ROS/NF‐ κ B/NLRP3 Inflammasome Pathway
    Article Snippet: β ‐Actin (Cat#R1207‐1) was purchased from Huabio (Harbin, China). .. Trypsin solution (Cat#C0201), CM‐H2DCFDA staining (Cat#S0035S), Cy3‐conjugated secondary antibody (Cat# A0521), FITC‐conjugated secondary antibody (Cat# A0568), 4 ′ ,6‐diamidino‐2‐phenylindole (DAPI) (CatC1005), RIPA (Cat#P0013B), protein SDS‐PAGE protein loading buffer (5X) (Cat#P0015), BCA protein concentration measurement kit (Cat#P0011), and lactate dehydrogenase (LDH) cytotoxicity assay kit (Cat#C0016) were purchased from Beyotime (Beijing, China). .. α ‐Actinin (Cat#GTX11007) was from GeneTex (Texas, United States).

    LDH Cytotoxicity Assay:

    Article Title: The AGEs/RAGE Axis Contributes to Myocardial Cell Death in Type 1 Diabetic Cardiomyopathy via the ROS/NF‐ κ B/NLRP3 Inflammasome Pathway
    Article Snippet: β ‐Actin (Cat#R1207‐1) was purchased from Huabio (Harbin, China). .. Trypsin solution (Cat#C0201), CM‐H2DCFDA staining (Cat#S0035S), Cy3‐conjugated secondary antibody (Cat# A0521), FITC‐conjugated secondary antibody (Cat# A0568), 4 ′ ,6‐diamidino‐2‐phenylindole (DAPI) (CatC1005), RIPA (Cat#P0013B), protein SDS‐PAGE protein loading buffer (5X) (Cat#P0015), BCA protein concentration measurement kit (Cat#P0011), and lactate dehydrogenase (LDH) cytotoxicity assay kit (Cat#C0016) were purchased from Beyotime (Beijing, China). .. α ‐Actinin (Cat#GTX11007) was from GeneTex (Texas, United States).



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    Image Search Results


    ( A ) Graphs depicting main cluster interactors based on incoming and outgoing interaction strengths in the tumor edge (left) and in the tumor core (right). ( B ) Representative immunofluorescent staining (left) and corresponding spatial plots (right) of CD8 + T cells (CD8 + ), monocytes (Mono, CD14 + IBA1 − ), and MoMs (CD14 + IBA1 + ) in the tumor edge and tumor core. Scale bars, 50 μm. ( C ) SpatialScore calculated from immunofluorescent data per patient in tumor edge and tumor core. Error bars denotes means ± SEM. P values, Wilcoxon rank-sum test. ( D ) Heatmap depicting selected ligand-receptor interactions from T cell clusters to THBS1 + monocytes (top) and LR interactions from THBS1 + monocytes to T cell clusters (bottom), enriched in the tumor edge. ( E ) Heatmap depicting selected LR interactions from THBS1 + monocytes to T cell clusters, enriched in the tumor edge. ( F ) Scatter plot shows the correlation between the abundance of SPP1 + macrophages and exhausted CD8 + T (CD8 + Tex) cells in the CRLM microarray dataset GSE159216 ( n = 171). The error band indicates the 95% confidence interval. In (D) and (E), color intensity represents the probability of communication. DAPI, 4′,6-diamidino-2-phenylindole.

    Journal: Science Advances

    Article Title: Multiregional profiling reveals THBS1 - SPP1 monocyte-macrophage axis drives immunosuppression and outcome in colorectal liver metastases

    doi: 10.1126/sciadv.aed1296

    Figure Lengend Snippet: ( A ) Graphs depicting main cluster interactors based on incoming and outgoing interaction strengths in the tumor edge (left) and in the tumor core (right). ( B ) Representative immunofluorescent staining (left) and corresponding spatial plots (right) of CD8 + T cells (CD8 + ), monocytes (Mono, CD14 + IBA1 − ), and MoMs (CD14 + IBA1 + ) in the tumor edge and tumor core. Scale bars, 50 μm. ( C ) SpatialScore calculated from immunofluorescent data per patient in tumor edge and tumor core. Error bars denotes means ± SEM. P values, Wilcoxon rank-sum test. ( D ) Heatmap depicting selected ligand-receptor interactions from T cell clusters to THBS1 + monocytes (top) and LR interactions from THBS1 + monocytes to T cell clusters (bottom), enriched in the tumor edge. ( E ) Heatmap depicting selected LR interactions from THBS1 + monocytes to T cell clusters, enriched in the tumor edge. ( F ) Scatter plot shows the correlation between the abundance of SPP1 + macrophages and exhausted CD8 + T (CD8 + Tex) cells in the CRLM microarray dataset GSE159216 ( n = 171). The error band indicates the 95% confidence interval. In (D) and (E), color intensity represents the probability of communication. DAPI, 4′,6-diamidino-2-phenylindole.

    Article Snippet: Sections were then incubated with primary antibodies at 4°C overnight followed by incubation with fluorophore-conjugated secondary antibodies (table S11) and nuclear dye 4′,6-diamidino-2-phenylindole (Thermo Fisher Scientific) at room temperature for 2 hours.

    Techniques: Staining, Microarray