Article Title: Regorafenib Induces Senescence and Epithelial-Mesenchymal Transition in Colorectal Cancer to Promote Drug Resistance
Figure Lengend Snippet: Regorafenib effect on Senescence-Associated β-galactosidase (SA-β-Gal) activity and cell cycle in in HCT-116, SW1116, LS1034, SW480 and Caco-2 cell lines after Regorafenib exposure. ( A , B ) SA-β-Gal activity assessment by detecting the SA-β-Gal substrate (fluorescein-di-beta-D-galactopyranoside (FDG)) by flow cytometry. ( C ) Proportion of cells detected in G0/G1, S and G2/M phase by flow cytometry through DNA staining (propidium iodide). Control; Rego-3d (short regorafenib exposure (3 days)); Rego-12m (long regorafenib exposure (12 months)). Data are expressed as means ± SEM (n ≥ 3) compared to control group with * p
Article Snippet: HCT-116 cell line was grown in McCoy’s 5A Medium (ATCC® 30-2007™), SW1116 in DMEM (Dulbecco/Vogt modified Eagle’s minimal essential medium), LS1034 in RPMI-1640 Medium (ATCC® 30-2001), SW480 cells in Leibovitz’s L-15 Medium (ATCC® 30-2008™) and Caco-2 in in Eagle’s Minimum Essential Medium (ATCC® 30-2003) all supplemented with 10% fetal bovine serum (FBS) in addition to 100 U/mL of penicillin and 100 µg/mL of streptomycin at standard concentrations (all from Thermo Fischer Scientific Gibco and Invitrogen Waltham, MA, USA).
Techniques: Activity Assay, Flow Cytometry, Staining