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  • 99
    ATCC dulbeccos s modified eagle s medium
    Dulbeccos S Modified Eagle S Medium, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dulbeccos s modified eagle s medium dmem
    Dulbeccos S Modified Eagle S Medium Dmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare dulbeccos s modified eagle s medium dmem
    Dulbeccos S Modified Eagle S Medium Dmem, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dulbeccos s modified eagle s medium
    Dulbeccos S Modified Eagle S Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Lonza complete dulbeccos s modified eagle s medium dmem
    Complete Dulbeccos S Modified Eagle S Medium Dmem, supplied by Lonza, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore glucose dulbeccos s modified eagle s medium
    Glucose Dulbeccos S Modified Eagle S Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mediatech dulbeccos s modified eagle s medium
    Dulbeccos S Modified Eagle S Medium, supplied by Mediatech, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fetal calf serum dulbeccos s modified eagle s medium
    Fetal Calf Serum Dulbeccos S Modified Eagle S Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore dulbeccos s modified eagle s medium
    Dulbeccos S Modified Eagle S Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dmem
    Populations of <t>PDPN+</t> cells among CAFs affected by environmental conditions. (A) The percentage of PDPN+ cells among CAFs changed in a time-dependent manner by culturing with <t>DMEM-containing</t> growth factors. (B) Populations of PDPN+ cells among CAFs changed by the addition of FBS in concentration- and time-dependent manners. The control (CTRL) condition consisted of DMEM containing 10% FBS and medium changes every day. (C) Populations of PDPN+ cells in cultures of serum-free DMEM increased more rapidly than those in cultures of glucose- and serum-free DMEM.
    Dmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 147958 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher advanced dmem
    Morphological characteristics and functional analysis of cell line–derived ESCC 3D organoids. ESCC 3D organoids were grown with TE11 cells in indicated cell culture media to acquire ( A ) phase contrast images and evaluate ( B ) organoid growth (size) at indicated time points. ( C ) Organoid formation rate was determined at day 11. RPMI1640 and <t>DMEM</t> are supplemented with 10% FBS. <t>aDMEM/F12</t> + is fully supplemented as used to grow patient-derived 3D organoids. Scale bar =100 μm. ( B ) Data are presented as mean ± SD of at least 7 organoids. Data represent 3 independent experiments with similar results and 1-way analysis of variance with multiple comparisons (Tukey) was performed for each ( B ) time point in and ( C ) condition. * P
    Advanced Dmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 856 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher advanced dmem f
    Morphological characteristics and functional analysis of cell line–derived ESCC 3D organoids. ESCC 3D organoids were grown with TE11 cells in indicated cell culture media to acquire ( A ) phase contrast images and evaluate ( B ) organoid growth (size) at indicated time points. ( C ) Organoid formation rate was determined at day 11. RPMI1640 and <t>DMEM</t> are supplemented with 10% FBS. <t>aDMEM/F12</t> + is fully supplemented as used to grow patient-derived 3D organoids. Scale bar =100 μm. ( B ) Data are presented as mean ± SD of at least 7 organoids. Data represent 3 independent experiments with similar results and 1-way analysis of variance with multiple comparisons (Tukey) was performed for each ( B ) time point in and ( C ) condition. * P
    Advanced Dmem F, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 207 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dmem medium
    Generation of FGF-iPS from mouse fibroblasts by retroviral transduction. Schematic diagram illustrating a timetable of the critical steps of the reprogramming protocol utilized. Embryonic mouse fibroblasts were transduced with retroviruses carrying Oct4, Sox2 Klf4 and c-Myc and then transferred onto MEF feeder layers in <t>DMEM</t> medium supplemented with 10% <t>FBS.</t> After 4 days the medium was switched to a human ESC culture medium. Colonies activated the Oct4-GFP transgene after twelve days in culture (B–C) and are isolated for expansion after 17–18 days (D–E). After picking, single clones give rise to stable cell lines of FGF-iPSCs, which grow as round compacted colonies (F, H) expressing the Oct4-GFP transgene (G, I). Colonies expressing the Oct4-GFP (K, O, S) result positive for alkaline phosphatase activity (AP) (J), and SSEA-1 (M), Sox2 (P) and Nanog (T) immunoistochemistry. Merged figures for Sox2/Oct4-GFP and Nanog/Oct4- immunostainings are showed in Q and U, respectively. DAPI fluorescent staining is labeling cell nuclei (blue) in L, N, R.
    Dmem Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 18072 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dmem fluorobrite
    Generation of FGF-iPS from mouse fibroblasts by retroviral transduction. Schematic diagram illustrating a timetable of the critical steps of the reprogramming protocol utilized. Embryonic mouse fibroblasts were transduced with retroviruses carrying Oct4, Sox2 Klf4 and c-Myc and then transferred onto MEF feeder layers in <t>DMEM</t> medium supplemented with 10% <t>FBS.</t> After 4 days the medium was switched to a human ESC culture medium. Colonies activated the Oct4-GFP transgene after twelve days in culture (B–C) and are isolated for expansion after 17–18 days (D–E). After picking, single clones give rise to stable cell lines of FGF-iPSCs, which grow as round compacted colonies (F, H) expressing the Oct4-GFP transgene (G, I). Colonies expressing the Oct4-GFP (K, O, S) result positive for alkaline phosphatase activity (AP) (J), and SSEA-1 (M), Sox2 (P) and Nanog (T) immunoistochemistry. Merged figures for Sox2/Oct4-GFP and Nanog/Oct4- immunostainings are showed in Q and U, respectively. DAPI fluorescent staining is labeling cell nuclei (blue) in L, N, R.
    Dmem Fluorobrite, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Thermo Fisher invitrogen dmem
    Generation of FGF-iPS from mouse fibroblasts by retroviral transduction. Schematic diagram illustrating a timetable of the critical steps of the reprogramming protocol utilized. Embryonic mouse fibroblasts were transduced with retroviruses carrying Oct4, Sox2 Klf4 and c-Myc and then transferred onto MEF feeder layers in <t>DMEM</t> medium supplemented with 10% <t>FBS.</t> After 4 days the medium was switched to a human ESC culture medium. Colonies activated the Oct4-GFP transgene after twelve days in culture (B–C) and are isolated for expansion after 17–18 days (D–E). After picking, single clones give rise to stable cell lines of FGF-iPSCs, which grow as round compacted colonies (F, H) expressing the Oct4-GFP transgene (G, I). Colonies expressing the Oct4-GFP (K, O, S) result positive for alkaline phosphatase activity (AP) (J), and SSEA-1 (M), Sox2 (P) and Nanog (T) immunoistochemistry. Merged figures for Sox2/Oct4-GFP and Nanog/Oct4- immunostainings are showed in Q and U, respectively. DAPI fluorescent staining is labeling cell nuclei (blue) in L, N, R.
    Invitrogen Dmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Populations of PDPN+ cells among CAFs affected by environmental conditions. (A) The percentage of PDPN+ cells among CAFs changed in a time-dependent manner by culturing with DMEM-containing growth factors. (B) Populations of PDPN+ cells among CAFs changed by the addition of FBS in concentration- and time-dependent manners. The control (CTRL) condition consisted of DMEM containing 10% FBS and medium changes every day. (C) Populations of PDPN+ cells in cultures of serum-free DMEM increased more rapidly than those in cultures of glucose- and serum-free DMEM.

    Journal: Molecular Cancer

    Article Title: Podoplanin expression in cancer-associated fibroblasts enhances tumor progression of invasive ductal carcinoma of the pancreas

    doi: 10.1186/1476-4598-12-168

    Figure Lengend Snippet: Populations of PDPN+ cells among CAFs affected by environmental conditions. (A) The percentage of PDPN+ cells among CAFs changed in a time-dependent manner by culturing with DMEM-containing growth factors. (B) Populations of PDPN+ cells among CAFs changed by the addition of FBS in concentration- and time-dependent manners. The control (CTRL) condition consisted of DMEM containing 10% FBS and medium changes every day. (C) Populations of PDPN+ cells in cultures of serum-free DMEM increased more rapidly than those in cultures of glucose- and serum-free DMEM.

    Article Snippet: In the PDPN induction assay, DMEM (Sigma Chemical Co., St. Louis, MO) or DMEM containing no glucose (Invitrogen, Carlsbad, CA) was used for cell culture.

    Techniques: Concentration Assay

    Morphological characteristics and functional analysis of cell line–derived ESCC 3D organoids. ESCC 3D organoids were grown with TE11 cells in indicated cell culture media to acquire ( A ) phase contrast images and evaluate ( B ) organoid growth (size) at indicated time points. ( C ) Organoid formation rate was determined at day 11. RPMI1640 and DMEM are supplemented with 10% FBS. aDMEM/F12 + is fully supplemented as used to grow patient-derived 3D organoids. Scale bar =100 μm. ( B ) Data are presented as mean ± SD of at least 7 organoids. Data represent 3 independent experiments with similar results and 1-way analysis of variance with multiple comparisons (Tukey) was performed for each ( B ) time point in and ( C ) condition. * P

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    Article Title: Three-Dimensional Organoids Reveal Therapy Resistance of Esophageal and Oropharyngeal Squamous Cell Carcinoma Cells

    doi: 10.1016/j.jcmgh.2018.09.003

    Figure Lengend Snippet: Morphological characteristics and functional analysis of cell line–derived ESCC 3D organoids. ESCC 3D organoids were grown with TE11 cells in indicated cell culture media to acquire ( A ) phase contrast images and evaluate ( B ) organoid growth (size) at indicated time points. ( C ) Organoid formation rate was determined at day 11. RPMI1640 and DMEM are supplemented with 10% FBS. aDMEM/F12 + is fully supplemented as used to grow patient-derived 3D organoids. Scale bar =100 μm. ( B ) Data are presented as mean ± SD of at least 7 organoids. Data represent 3 independent experiments with similar results and 1-way analysis of variance with multiple comparisons (Tukey) was performed for each ( B ) time point in and ( C ) condition. * P

    Article Snippet: Cells were then resuspended in 1-mL advanced Dulbecco’s modified Eagle medium (DMEM)/F12 (12491015, Thermo Fisher Scientific) containing 100-U/mL penicillin and 100-μg/mL streptomycin (15140122, Thermo Fisher Scientific), 1×GlutaMAX (35050061, Thermo Fisher Scientific), and 1×HEPES (15630080, Thermo Fisher Scientific) (aDMEM/F12+ ).

    Techniques: Functional Assay, Derivative Assay, Cell Culture

    Generation of FGF-iPS from mouse fibroblasts by retroviral transduction. Schematic diagram illustrating a timetable of the critical steps of the reprogramming protocol utilized. Embryonic mouse fibroblasts were transduced with retroviruses carrying Oct4, Sox2 Klf4 and c-Myc and then transferred onto MEF feeder layers in DMEM medium supplemented with 10% FBS. After 4 days the medium was switched to a human ESC culture medium. Colonies activated the Oct4-GFP transgene after twelve days in culture (B–C) and are isolated for expansion after 17–18 days (D–E). After picking, single clones give rise to stable cell lines of FGF-iPSCs, which grow as round compacted colonies (F, H) expressing the Oct4-GFP transgene (G, I). Colonies expressing the Oct4-GFP (K, O, S) result positive for alkaline phosphatase activity (AP) (J), and SSEA-1 (M), Sox2 (P) and Nanog (T) immunoistochemistry. Merged figures for Sox2/Oct4-GFP and Nanog/Oct4- immunostainings are showed in Q and U, respectively. DAPI fluorescent staining is labeling cell nuclei (blue) in L, N, R.

    Journal: PLoS ONE

    Article Title: An ES-Like Pluripotent State in FGF-Dependent Murine iPS cells

    doi: 10.1371/journal.pone.0016092

    Figure Lengend Snippet: Generation of FGF-iPS from mouse fibroblasts by retroviral transduction. Schematic diagram illustrating a timetable of the critical steps of the reprogramming protocol utilized. Embryonic mouse fibroblasts were transduced with retroviruses carrying Oct4, Sox2 Klf4 and c-Myc and then transferred onto MEF feeder layers in DMEM medium supplemented with 10% FBS. After 4 days the medium was switched to a human ESC culture medium. Colonies activated the Oct4-GFP transgene after twelve days in culture (B–C) and are isolated for expansion after 17–18 days (D–E). After picking, single clones give rise to stable cell lines of FGF-iPSCs, which grow as round compacted colonies (F, H) expressing the Oct4-GFP transgene (G, I). Colonies expressing the Oct4-GFP (K, O, S) result positive for alkaline phosphatase activity (AP) (J), and SSEA-1 (M), Sox2 (P) and Nanog (T) immunoistochemistry. Merged figures for Sox2/Oct4-GFP and Nanog/Oct4- immunostainings are showed in Q and U, respectively. DAPI fluorescent staining is labeling cell nuclei (blue) in L, N, R.

    Article Snippet: Single cells and small aggregates were cultured and propagated in DMEM medium (Invitrogen) with 10% FBS (Sigma).

    Techniques: Transduction, Isolation, Clone Assay, Stable Transfection, Expressing, Activity Assay, Staining, Labeling

    In vitro and in vivo pluripotency of FGF-iPSCs. (A) FGF-iPSCs were detached with collagenase IV from the substrate to induce embryoid body (EB) formation. After 5 days in culture, the majority of EBs downregulate the GFP transgene. (B) EBs plated in N2-medium generate nestin positive neural progenitor cells as highlighted by immunofluorescence with its specific antibody. (C) EBs were plated on gelatin coated dishes containing DMEM medium supplemented with 20% FBS. After 20 days in such condition, islands of beating cardiomyocite became evident (see Movie S1 ). Cell differentiation was confirmed by immunoistochemistry for the mesodermal marker smooth muscle actin (Sma) (C) and for the endodermal marker Sox17 (D). (E–H) Ematoxylin and Eosin histological staining of teratomas, derived by subcutaneously injection of 1 million FGF-iPSCs, showed cartilage (E), adipose tissue (F), gut-like epithelium (G) and muscle (H). (I) Oct4-GFP positive FGF-iPSCs integrate into the inner cell mass of mouse blastocyst after morula aggregation and contribute, thereafter, to viable highly chimaeric animals (on the right) as compared to unmanipulated mice (on the left) (J). (K) Germiline contribution of FGF-iPS #5 and #9 chimera. (L) Genotyping demontrate the presence of the Oct4-GFP transgene in the offspring.

    Journal: PLoS ONE

    Article Title: An ES-Like Pluripotent State in FGF-Dependent Murine iPS cells

    doi: 10.1371/journal.pone.0016092

    Figure Lengend Snippet: In vitro and in vivo pluripotency of FGF-iPSCs. (A) FGF-iPSCs were detached with collagenase IV from the substrate to induce embryoid body (EB) formation. After 5 days in culture, the majority of EBs downregulate the GFP transgene. (B) EBs plated in N2-medium generate nestin positive neural progenitor cells as highlighted by immunofluorescence with its specific antibody. (C) EBs were plated on gelatin coated dishes containing DMEM medium supplemented with 20% FBS. After 20 days in such condition, islands of beating cardiomyocite became evident (see Movie S1 ). Cell differentiation was confirmed by immunoistochemistry for the mesodermal marker smooth muscle actin (Sma) (C) and for the endodermal marker Sox17 (D). (E–H) Ematoxylin and Eosin histological staining of teratomas, derived by subcutaneously injection of 1 million FGF-iPSCs, showed cartilage (E), adipose tissue (F), gut-like epithelium (G) and muscle (H). (I) Oct4-GFP positive FGF-iPSCs integrate into the inner cell mass of mouse blastocyst after morula aggregation and contribute, thereafter, to viable highly chimaeric animals (on the right) as compared to unmanipulated mice (on the left) (J). (K) Germiline contribution of FGF-iPS #5 and #9 chimera. (L) Genotyping demontrate the presence of the Oct4-GFP transgene in the offspring.

    Article Snippet: Single cells and small aggregates were cultured and propagated in DMEM medium (Invitrogen) with 10% FBS (Sigma).

    Techniques: In Vitro, In Vivo, Immunofluorescence, Cell Differentiation, Marker, Staining, Derivative Assay, Injection, Mouse Assay