dmem Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    ATCC dulbeccos s modified eagle s medium
    Dulbeccos S Modified Eagle S Medium, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dulbeccos s modified eagle s medium/product/ATCC
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    dulbeccos s modified eagle s medium - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher dulbeccos s modified eagle s medium dmem
    Dulbeccos S Modified Eagle S Medium Dmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dulbeccos s modified eagle s medium dmem/product/Thermo Fisher
    Average 99 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    dulbeccos s modified eagle s medium dmem - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    90
    GE Healthcare dulbeccos s modified eagle s medium dmem
    Dulbeccos S Modified Eagle S Medium Dmem, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dulbeccos s modified eagle s medium dmem/product/GE Healthcare
    Average 90 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    dulbeccos s modified eagle s medium dmem - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    99
    Thermo Fisher dulbeccos s modified eagle s medium
    Dulbeccos S Modified Eagle S Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dulbeccos s modified eagle s medium/product/Thermo Fisher
    Average 99 stars, based on 51 article reviews
    Price from $9.99 to $1999.99
    dulbeccos s modified eagle s medium - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    92
    Lonza complete dulbeccos s modified eagle s medium dmem
    Complete Dulbeccos S Modified Eagle S Medium Dmem, supplied by Lonza, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complete dulbeccos s modified eagle s medium dmem/product/Lonza
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    complete dulbeccos s modified eagle s medium dmem - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    99
    Millipore glucose dulbeccos s modified eagle s medium
    Glucose Dulbeccos S Modified Eagle S Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glucose dulbeccos s modified eagle s medium/product/Millipore
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    glucose dulbeccos s modified eagle s medium - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    88
    Mediatech dulbeccos s modified eagle s medium
    Dulbeccos S Modified Eagle S Medium, supplied by Mediatech, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dulbeccos s modified eagle s medium/product/Mediatech
    Average 88 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    dulbeccos s modified eagle s medium - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    99
    Thermo Fisher fetal calf serum dulbeccos s modified eagle s medium
    Fetal Calf Serum Dulbeccos S Modified Eagle S Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fetal calf serum dulbeccos s modified eagle s medium/product/Thermo Fisher
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    fetal calf serum dulbeccos s modified eagle s medium - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Millipore dulbeccos s modified eagle s medium
    Dulbeccos S Modified Eagle S Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dulbeccos s modified eagle s medium/product/Millipore
    Average 99 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    dulbeccos s modified eagle s medium - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher dmem
    Signaling molecules involved in ADM actions on PDX1– <t>β</t> -Lox5 cell aggregates. Aggregated β -Lox-5 cells transduced with PDX1 on day 7 of culture in <t>DMEM</t> containing 4 mg/mL glucose were treated with (A) KT5720 (KT, 10 µM), (B) PD98059 (PD, 10 µM), or (C) wortmannin (Wort, 0.1 µM) for 30 min and then ADM (0.1 µM) for 24 h, and insulin concentrations in the cell culture media were determined using human insulin ELISA kit. Data are displayed as mean ± SEM of the results from three separate experiments. Different letters indicate significant differences between groups ( P > 0.05).
    Dmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 148083 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmem/product/Thermo Fisher
    Average 99 stars, based on 148083 article reviews
    Price from $9.99 to $1999.99
    dmem - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher advanced dmem a dmem
    Morphological characteristics and functional analysis of cell line–derived ESCC 3D organoids. ESCC 3D organoids were grown with TE11 cells in indicated cell culture media to acquire ( A ) phase contrast images and evaluate ( B ) organoid growth (size) at indicated time points. ( C ) Organoid formation rate was determined at day 11. RPMI1640 and <t>DMEM</t> are supplemented with 10% FBS. <t>aDMEM/F12</t> + is fully supplemented as used to grow patient-derived 3D organoids. Scale bar =100 μm. ( B ) Data are presented as mean ± SD of at least 7 organoids. Data represent 3 independent experiments with similar results and 1-way analysis of variance with multiple comparisons (Tukey) was performed for each ( B ) time point in and ( C ) condition. * P
    Advanced Dmem A Dmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/advanced dmem a dmem/product/Thermo Fisher
    Average 99 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    advanced dmem a dmem - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher dmem fluorobrite
    Morphological characteristics and functional analysis of cell line–derived ESCC 3D organoids. ESCC 3D organoids were grown with TE11 cells in indicated cell culture media to acquire ( A ) phase contrast images and evaluate ( B ) organoid growth (size) at indicated time points. ( C ) Organoid formation rate was determined at day 11. RPMI1640 and <t>DMEM</t> are supplemented with 10% FBS. <t>aDMEM/F12</t> + is fully supplemented as used to grow patient-derived 3D organoids. Scale bar =100 μm. ( B ) Data are presented as mean ± SD of at least 7 organoids. Data represent 3 independent experiments with similar results and 1-way analysis of variance with multiple comparisons (Tukey) was performed for each ( B ) time point in and ( C ) condition. * P
    Dmem Fluorobrite, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmem fluorobrite/product/Thermo Fisher
    Average 99 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    dmem fluorobrite - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Signaling molecules involved in ADM actions on PDX1– β -Lox5 cell aggregates. Aggregated β -Lox-5 cells transduced with PDX1 on day 7 of culture in DMEM containing 4 mg/mL glucose were treated with (A) KT5720 (KT, 10 µM), (B) PD98059 (PD, 10 µM), or (C) wortmannin (Wort, 0.1 µM) for 30 min and then ADM (0.1 µM) for 24 h, and insulin concentrations in the cell culture media were determined using human insulin ELISA kit. Data are displayed as mean ± SEM of the results from three separate experiments. Different letters indicate significant differences between groups ( P > 0.05).

    Journal: The Journal of Clinical Endocrinology and Metabolism

    Article Title: Circulating Adrenomedullin Is Elevated in Gestational Diabetes and Its Role in Impaired Insulin Production by β-Cells

    doi: 10.1210/jc.2018-01119

    Figure Lengend Snippet: Signaling molecules involved in ADM actions on PDX1– β -Lox5 cell aggregates. Aggregated β -Lox-5 cells transduced with PDX1 on day 7 of culture in DMEM containing 4 mg/mL glucose were treated with (A) KT5720 (KT, 10 µM), (B) PD98059 (PD, 10 µM), or (C) wortmannin (Wort, 0.1 µM) for 30 min and then ADM (0.1 µM) for 24 h, and insulin concentrations in the cell culture media were determined using human insulin ELISA kit. Data are displayed as mean ± SEM of the results from three separate experiments. Different letters indicate significant differences between groups ( P > 0.05).

    Article Snippet: The β -Lox5 cell line, derived from human pancreatic β -cells, was kindly provided by Dr. Clayton Mathews from the University of Florida College of Medicine. β -Lox5 cells were maintained in low glucose (1 mg/mL) DMEM (Gibco, Life Technology, Gaithersburg, MD) supplemented with 10% fetal bovine serum (HyClone; Fisher Scientific, Pittsburgh, PA), 1% MEM nonessential amino acids (Gibco), 1% penicillin-streptomycin (Gemini Bio-Products, West Sacramento, CA), 0.02% BSA (Sigma, St. Louis, MO), and 15 mM HEPES (Gibco).

    Techniques: Transduction, Cell Culture, Enzyme-linked Immunosorbent Assay

    ADM inhibits insulin mRNA and secretion in PDX1– β -Lox5 cell aggregates. (A) mRNA for insulin in monolayer (Mono) or aggregated (Aggr) β -Lox-5 cells transduced with or without PDX1 on day 7 of culture in DMEM containing 4 mg/mL glucose was determined using real-time quantitative PCR and normalized to GAPDH mRNA expression. (B) Aggregated β -Lox-5 cells transduced with PDX1 on day 7 of culture in DMEM containing 4 mg/mL glucose were treated with ADM in the presence or absence of ADM22-52 for 24 h, and mRNA for insulin in the cells was determined using real-time quantitative PCR and normalized to GAPDH mRNA expression. (C) Insulin concentration in cell culture medium from (B) was determined using a human insulin ELISA kit. Data are displayed as mean ± SEM of the results from three separate experiments. Different letters indicate significant differences between groups ( P > 0.05).

    Journal: The Journal of Clinical Endocrinology and Metabolism

    Article Title: Circulating Adrenomedullin Is Elevated in Gestational Diabetes and Its Role in Impaired Insulin Production by β-Cells

    doi: 10.1210/jc.2018-01119

    Figure Lengend Snippet: ADM inhibits insulin mRNA and secretion in PDX1– β -Lox5 cell aggregates. (A) mRNA for insulin in monolayer (Mono) or aggregated (Aggr) β -Lox-5 cells transduced with or without PDX1 on day 7 of culture in DMEM containing 4 mg/mL glucose was determined using real-time quantitative PCR and normalized to GAPDH mRNA expression. (B) Aggregated β -Lox-5 cells transduced with PDX1 on day 7 of culture in DMEM containing 4 mg/mL glucose were treated with ADM in the presence or absence of ADM22-52 for 24 h, and mRNA for insulin in the cells was determined using real-time quantitative PCR and normalized to GAPDH mRNA expression. (C) Insulin concentration in cell culture medium from (B) was determined using a human insulin ELISA kit. Data are displayed as mean ± SEM of the results from three separate experiments. Different letters indicate significant differences between groups ( P > 0.05).

    Article Snippet: The β -Lox5 cell line, derived from human pancreatic β -cells, was kindly provided by Dr. Clayton Mathews from the University of Florida College of Medicine. β -Lox5 cells were maintained in low glucose (1 mg/mL) DMEM (Gibco, Life Technology, Gaithersburg, MD) supplemented with 10% fetal bovine serum (HyClone; Fisher Scientific, Pittsburgh, PA), 1% MEM nonessential amino acids (Gibco), 1% penicillin-streptomycin (Gemini Bio-Products, West Sacramento, CA), 0.02% BSA (Sigma, St. Louis, MO), and 15 mM HEPES (Gibco).

    Techniques: Transduction, Real-time Polymerase Chain Reaction, Expressing, Concentration Assay, Cell Culture, Enzyme-linked Immunosorbent Assay

    Analysis of c-Fos and c-Jun protein expression in cervical cancer cells. Total cellular proteins were obtained from 1 × 10 5 HaCaT (lane 1), C-33A (lane 2), SiHa (lane 3) and HeLa cells (lane 4) per well in a six-well plate containing DMEM at 37 °C with 10% FBS in 5% CO 2 . The proteins were separated in 12% SDS-PAGE and were transferred to nitrocellulose membranes, which were incubated with each antibody. a The amount of similar proteins analyzed in the immunoblots. The anti-beta-actin antibody was included as control. b The immunoblot bands digitalized and analyzed by densitometer. The data were analyzed by c-Fos/c-Jun/beta-actin fold change in relative expression units (mean ± SE), not statistically significant (ns) and P values

    Journal: Cancer Cell International

    Article Title: Transregulation of microRNA miR-21 promoter by AP-1 transcription factor in cervical cancer cells

    doi: 10.1186/s12935-019-0931-x

    Figure Lengend Snippet: Analysis of c-Fos and c-Jun protein expression in cervical cancer cells. Total cellular proteins were obtained from 1 × 10 5 HaCaT (lane 1), C-33A (lane 2), SiHa (lane 3) and HeLa cells (lane 4) per well in a six-well plate containing DMEM at 37 °C with 10% FBS in 5% CO 2 . The proteins were separated in 12% SDS-PAGE and were transferred to nitrocellulose membranes, which were incubated with each antibody. a The amount of similar proteins analyzed in the immunoblots. The anti-beta-actin antibody was included as control. b The immunoblot bands digitalized and analyzed by densitometer. The data were analyzed by c-Fos/c-Jun/beta-actin fold change in relative expression units (mean ± SE), not statistically significant (ns) and P values

    Article Snippet: The cell lines were cultured in DMEM medium (Invitrogen, Carlsbad, CA) with 10% FBS, 100 U/ml penicillin/100 μg/ml streptomycin, 2 mM l -glutamine, 250 ng/ml fungizone, and maintained at 37 °C in 5% CO2.

    Techniques: Expressing, SDS Page, Incubation, Western Blot

    MiR-21 gene expression analysis after constitutive activation of AP-1. Total RNA and cDNA synthesis were obtained from 1 × 10 5 SiHa and HaCaT cells per well in a six-well plate containing DMEM at 37 °C with 10% FBS in 5% CO 2 after 60 min of non-treatment (NT) or treatment with 10 ng/ml PMA and 50 μM SR11302. The miR-21 gene expression was analyzed by real-time qRT-PCR and relative expression of miR-21 was calculated using the 2 −∆∆Ct method. The RNU44 gene expression was used as control. The data were analyzed by mRNA miR-21/mRNA RNU44 ratio in relative expression units. The values are presented as mean ± SD and P values

    Journal: Cancer Cell International

    Article Title: Transregulation of microRNA miR-21 promoter by AP-1 transcription factor in cervical cancer cells

    doi: 10.1186/s12935-019-0931-x

    Figure Lengend Snippet: MiR-21 gene expression analysis after constitutive activation of AP-1. Total RNA and cDNA synthesis were obtained from 1 × 10 5 SiHa and HaCaT cells per well in a six-well plate containing DMEM at 37 °C with 10% FBS in 5% CO 2 after 60 min of non-treatment (NT) or treatment with 10 ng/ml PMA and 50 μM SR11302. The miR-21 gene expression was analyzed by real-time qRT-PCR and relative expression of miR-21 was calculated using the 2 −∆∆Ct method. The RNU44 gene expression was used as control. The data were analyzed by mRNA miR-21/mRNA RNU44 ratio in relative expression units. The values are presented as mean ± SD and P values

    Article Snippet: The cell lines were cultured in DMEM medium (Invitrogen, Carlsbad, CA) with 10% FBS, 100 U/ml penicillin/100 μg/ml streptomycin, 2 mM l -glutamine, 250 ng/ml fungizone, and maintained at 37 °C in 5% CO2.

    Techniques: Expressing, Activation Assay, Quantitative RT-PCR

    Analysis of c-Fos and c-Jun protein expression after constitutive activation of AP-1. Total cellular proteins were obtained from 1 × 10 5 SiHa ( a ) and HaCaT cells ( b ) per well in a six-well plate containing DMEM at 37 °C with 10% FBS in 5% CO 2 after 0, 24, 48 and 72 h treated with 10 ng/ml PMA (lanes 1–4) or 50 μM SR11302 (lanes 5–8). The proteins were separated in 12% SDS-PAGE and were transferred to nitrocellulose membranes, which were incubated with each antibody. Similar amount of proteins were analyzed in the immunoblots. The anti-beta-actin antibody was included as control. The immunoblot bands were digitalized and analyzed by densitometer. The data were analyzed by c-Fos/c-Jun/beta-actin fold change in relative expression units (mean ± SE), not statistically significant (ns) and P values

    Journal: Cancer Cell International

    Article Title: Transregulation of microRNA miR-21 promoter by AP-1 transcription factor in cervical cancer cells

    doi: 10.1186/s12935-019-0931-x

    Figure Lengend Snippet: Analysis of c-Fos and c-Jun protein expression after constitutive activation of AP-1. Total cellular proteins were obtained from 1 × 10 5 SiHa ( a ) and HaCaT cells ( b ) per well in a six-well plate containing DMEM at 37 °C with 10% FBS in 5% CO 2 after 0, 24, 48 and 72 h treated with 10 ng/ml PMA (lanes 1–4) or 50 μM SR11302 (lanes 5–8). The proteins were separated in 12% SDS-PAGE and were transferred to nitrocellulose membranes, which were incubated with each antibody. Similar amount of proteins were analyzed in the immunoblots. The anti-beta-actin antibody was included as control. The immunoblot bands were digitalized and analyzed by densitometer. The data were analyzed by c-Fos/c-Jun/beta-actin fold change in relative expression units (mean ± SE), not statistically significant (ns) and P values

    Article Snippet: The cell lines were cultured in DMEM medium (Invitrogen, Carlsbad, CA) with 10% FBS, 100 U/ml penicillin/100 μg/ml streptomycin, 2 mM l -glutamine, 250 ng/ml fungizone, and maintained at 37 °C in 5% CO2.

    Techniques: Expressing, Activation Assay, SDS Page, Incubation, Western Blot

    Changes in EB1-EGFP comet movement in EB1-EGFP-KPL cells after treatment with DM1, an inhibitor of microtubule polymerization, in vitro . DM1 (Carbosynth, UK) used for the T-DM1 control study was diluted to 10 mM in dimethyl sulfoxide (Thermo Fisher Scientific) and then diluted to 1 μM in FluoroBrite DMEM. EB1-EGFP-KPL cells were treated with 0, 0.01, 0.1, 1, 10, 100, or 1000 nM DM1 for 10 minutes and then observed using time-lapse images taken over 20 seconds, with an exposure time of 1.07 s/frame and no delay time. The time-lapse images were created using maximum intensity projection methods to overlay all the fluorescence images. The top and bottom rows present time-lapse images of EB1-EGFP-KPL cells before or after treatment with various concentrations of DM1 for 10 min. Bar, 10 μm.

    Journal: Translational Oncology

    Article Title: Heterogeneous Drug Efficacy of an Antibody-Drug Conjugate Visualized Using Simultaneous Imaging of Its Delivery and Intracellular Damage in Living Tumor Tissues

    doi: 10.1016/j.tranon.2020.100764

    Figure Lengend Snippet: Changes in EB1-EGFP comet movement in EB1-EGFP-KPL cells after treatment with DM1, an inhibitor of microtubule polymerization, in vitro . DM1 (Carbosynth, UK) used for the T-DM1 control study was diluted to 10 mM in dimethyl sulfoxide (Thermo Fisher Scientific) and then diluted to 1 μM in FluoroBrite DMEM. EB1-EGFP-KPL cells were treated with 0, 0.01, 0.1, 1, 10, 100, or 1000 nM DM1 for 10 minutes and then observed using time-lapse images taken over 20 seconds, with an exposure time of 1.07 s/frame and no delay time. The time-lapse images were created using maximum intensity projection methods to overlay all the fluorescence images. The top and bottom rows present time-lapse images of EB1-EGFP-KPL cells before or after treatment with various concentrations of DM1 for 10 min. Bar, 10 μm.

    Article Snippet: The excised tumors were washed with FluoroBrite DMEM and placed in a 37°C environment.

    Techniques: In Vitro, Fluorescence

    Morphological characteristics and functional analysis of cell line–derived ESCC 3D organoids. ESCC 3D organoids were grown with TE11 cells in indicated cell culture media to acquire ( A ) phase contrast images and evaluate ( B ) organoid growth (size) at indicated time points. ( C ) Organoid formation rate was determined at day 11. RPMI1640 and DMEM are supplemented with 10% FBS. aDMEM/F12 + is fully supplemented as used to grow patient-derived 3D organoids. Scale bar =100 μm. ( B ) Data are presented as mean ± SD of at least 7 organoids. Data represent 3 independent experiments with similar results and 1-way analysis of variance with multiple comparisons (Tukey) was performed for each ( B ) time point in and ( C ) condition. * P

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    Article Title: Three-Dimensional Organoids Reveal Therapy Resistance of Esophageal and Oropharyngeal Squamous Cell Carcinoma Cells

    doi: 10.1016/j.jcmgh.2018.09.003

    Figure Lengend Snippet: Morphological characteristics and functional analysis of cell line–derived ESCC 3D organoids. ESCC 3D organoids were grown with TE11 cells in indicated cell culture media to acquire ( A ) phase contrast images and evaluate ( B ) organoid growth (size) at indicated time points. ( C ) Organoid formation rate was determined at day 11. RPMI1640 and DMEM are supplemented with 10% FBS. aDMEM/F12 + is fully supplemented as used to grow patient-derived 3D organoids. Scale bar =100 μm. ( B ) Data are presented as mean ± SD of at least 7 organoids. Data represent 3 independent experiments with similar results and 1-way analysis of variance with multiple comparisons (Tukey) was performed for each ( B ) time point in and ( C ) condition. * P

    Article Snippet: Cells were then resuspended in 1-mL advanced Dulbecco’s modified Eagle medium (DMEM)/F12 (12491015, Thermo Fisher Scientific) containing 100-U/mL penicillin and 100-μg/mL streptomycin (15140122, Thermo Fisher Scientific), 1×GlutaMAX (35050061, Thermo Fisher Scientific), and 1×HEPES (15630080, Thermo Fisher Scientific) (aDMEM/F12+ ).

    Techniques: Functional Assay, Derivative Assay, Cell Culture