assistant_photoSave product to folder
X
Create new folder
Overview
Images
Article Snippets
 86 / 100 Bioz Stars | Bio-Techne corporation / Advanced Cell Diag  | Visit Supplier |
Open an account
Product Images (7) - All
All
Comparison
Expressing
Imaging
Immunohistochemistry
In Situ Hybridization
Labeling
Saline
Sequencing
Staining
Transfection
Transgenic Assay
Relapse-Associated Transient Synaptic Potentiation Requires Integrin-Mediated Activation of Focal Adhesion Kinase and Cofilin in D1-Expressing Neurons
star_border
![Increased p-FAK expression in <t>D1-MSN</t> and D2-MSN, but p-cofilin only showed increased expression in D1-MSN during cue-induced cocaine seeking. p-FAK colocalization: A, D, Dendritic segments in NAcore labeled with mCherry-ChR2 (green, pseudo-colored images) in D1-MSN and D2-MSN and p-FAK (red) in each treatment group [saline (SAL), extinguished (EXT), reinstated (RST)]. Micrograph pairs of each segment depict the confocal imaging showing p-FAK puncta and labeled dendrites (left), and p-FAK puncta within the dendrite (right). Scale bar = 5 µm. B, Increased dendritic p-FAK immunoreactivity within D1-MSN dendrites in reinstated group compared with saline and extinguished animals. C, Positive correlation between p-FAK puncta expression within D1-MSN dendrite and active lever presses during 15-min cue-induced reinstatement. E, Increased dendritic p-FAK immunoreactivity within D2-MSN dendrites in reinstated group compared with saline and extinguished animals. F, No correlation between p-FAK puncta expression within the D2-MSN dendrite and active lever presses during cued reinstatement. p-cofilin colocalization: G, I, Dendritic segments in NAcore labeled with mCherry-ChR2 (green, pseudo-colored images) in D1-MSN and D2-MSN and p-cofilin (red) in each treatment group (SAL, EXT, RST). Micrograph pairs of each segment depict the confocal imaging showing p-cofilin puncta and labeled dendrites (left), and p-coflin puncta within the dendrite (right). Scale bar = 5 µm. H, Increased dendritic p-cofilin immunoreactivity within D1-MSN dendrites in reinstated group compared with saline and extinguished animals. J, No significant difference observed in p-cofilin puncta expression in D2-MSN dendrites. Data are shown as mean ± SEM N represents number of neurons quantified over number of animals in each condition; *p < 0.05 comparing RST to SAL; #p < 0.05 compared RST to EXT using Bonferroni post hoc.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5418/pmc07605418/pmc07605418__SN-JNSJ200542F009.jpg)
Relapse-Associated Transient Synaptic Potentiation Requires Integrin-Mediated Activation of Focal Adhesion Kinase and Cofilin in D1-Expressing Neurons
star_border
![Increased p-FAK expression in both <t>D1-MSN</t> and D2-MSN cell bodies, while p-cofilin showed increased expression only in D1-MSN cell bodies during cue-induced cocaine seeking. p-FAK colocalization: A, C, Cell bodies in NAcore labeled with mCherry-ChR2 (green, pseudo-colored images) in D1-MSN and D2-MSN and p-FAK (red) in each treatment group [saline (SAL), extinguished (EXT), reinstated (RST)]. Micrographs of each cell body depict the confocal images of p-FAK puncta and labeled cell bodies (left), and p-FAK puncta within the cell body (right). Scale bar = 30 µm. B, Increased cell body p-FAK immunoreactivity within D1-MSN in extinguished and reinstated group compared with saline animals. D, Increased cell body p-FAK immunoreactivity within D1-MSN in reinstated group compared with saline and extinguished animals. p-cofilin colocalization: E, G, Cell bodies in NAcore labeled with mCherry-ChR2 (green, pseudo-colored images) in D1-MSN and D2-MSN and p-cofilin (red) in each treatment group (SAL, EXT, RST). Micrographs of each cell body depict the confocal images of p-cofilin puncta and labeled cell bodies (left), and p-cofilin puncta within the cell body expressed as % colocalized (right). Scale bar = 30 µm. F, Increased cell body p-cofilin immunoreactivity within D1-MSN in reinstated group compared with saline and extinguished animals. H, No significant difference observed in p-cofilin puncta expression in D2-MSN cell body. Data are shown as mean ± SEM; N represents number of cell bodies quantified over number of animals in each condition; *p < 0.05 comparing RST or EXT to SAL; #p < 0.05 compared RST to EXT using Bonferroni post hoc.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5418/pmc07605418/pmc07605418__SN-JNSJ200542F010.jpg)
Relapse-Associated Transient Synaptic Potentiation Requires Integrin-Mediated Activation of Focal Adhesion Kinase and Cofilin in D1-Expressing Neurons
star_border
×![]()
Relapse-Associated Transient Synaptic Potentiation Requires Integrin-Mediated Activation of Focal Adhesion Kinase and Cofilin in D1-Expressing Neurons
Product Images (7)
star_border
![Increased p-FAK expression in <t>D1-MSN</t> and D2-MSN, but p-cofilin only showed increased expression in D1-MSN during cue-induced cocaine seeking. p-FAK colocalization: A, D, Dendritic segments in NAcore labeled with mCherry-ChR2 (green, pseudo-colored images) in D1-MSN and D2-MSN and p-FAK (red) in each treatment group [saline (SAL), extinguished (EXT), reinstated (RST)]. Micrograph pairs of each segment depict the confocal imaging showing p-FAK puncta and labeled dendrites (left), and p-FAK puncta within the dendrite (right). Scale bar = 5 µm. B, Increased dendritic p-FAK immunoreactivity within D1-MSN dendrites in reinstated group compared with saline and extinguished animals. C, Positive correlation between p-FAK puncta expression within D1-MSN dendrite and active lever presses during 15-min cue-induced reinstatement. E, Increased dendritic p-FAK immunoreactivity within D2-MSN dendrites in reinstated group compared with saline and extinguished animals. F, No correlation between p-FAK puncta expression within the D2-MSN dendrite and active lever presses during cued reinstatement. p-cofilin colocalization: G, I, Dendritic segments in NAcore labeled with mCherry-ChR2 (green, pseudo-colored images) in D1-MSN and D2-MSN and p-cofilin (red) in each treatment group (SAL, EXT, RST). Micrograph pairs of each segment depict the confocal imaging showing p-cofilin puncta and labeled dendrites (left), and p-coflin puncta within the dendrite (right). Scale bar = 5 µm. H, Increased dendritic p-cofilin immunoreactivity within D1-MSN dendrites in reinstated group compared with saline and extinguished animals. J, No significant difference observed in p-cofilin puncta expression in D2-MSN dendrites. Data are shown as mean ± SEM N represents number of neurons quantified over number of animals in each condition; *p < 0.05 comparing RST to SAL; #p < 0.05 compared RST to EXT using Bonferroni post hoc.](https://pub-med-central-thumbnail-images-cdn.bioz.com/pub_med_central_ids_ending_with_5418/pmc07605418/pmc07605418__SN-JNSJ200542F009.thumbnail.jpg)
![Increased p-FAK expression in both <t>D1-MSN</t> and D2-MSN cell bodies, while p-cofilin showed increased expression only in D1-MSN cell bodies during cue-induced cocaine seeking. p-FAK colocalization: A, C, Cell bodies in NAcore labeled with mCherry-ChR2 (green, pseudo-colored images) in D1-MSN and D2-MSN and p-FAK (red) in each treatment group [saline (SAL), extinguished (EXT), reinstated (RST)]. Micrographs of each cell body depict the confocal images of p-FAK puncta and labeled cell bodies (left), and p-FAK puncta within the cell body (right). Scale bar = 30 µm. B, Increased cell body p-FAK immunoreactivity within D1-MSN in extinguished and reinstated group compared with saline animals. D, Increased cell body p-FAK immunoreactivity within D1-MSN in reinstated group compared with saline and extinguished animals. p-cofilin colocalization: E, G, Cell bodies in NAcore labeled with mCherry-ChR2 (green, pseudo-colored images) in D1-MSN and D2-MSN and p-cofilin (red) in each treatment group (SAL, EXT, RST). Micrographs of each cell body depict the confocal images of p-cofilin puncta and labeled cell bodies (left), and p-cofilin puncta within the cell body expressed as % colocalized (right). Scale bar = 30 µm. F, Increased cell body p-cofilin immunoreactivity within D1-MSN in reinstated group compared with saline and extinguished animals. H, No significant difference observed in p-cofilin puncta expression in D2-MSN cell body. Data are shown as mean ± SEM; N represents number of cell bodies quantified over number of animals in each condition; *p < 0.05 comparing RST or EXT to SAL; #p < 0.05 compared RST to EXT using Bonferroni post hoc.](https://pub-med-central-thumbnail-images-cdn.bioz.com/pub_med_central_ids_ending_with_5418/pmc07605418/pmc07605418__SN-JNSJ200542F010.thumbnail.jpg)
Article Snippet
"For the detection of D1 receptor (Drd1a), D2 receptor (Drd2), or Cre recombinase mRNA, the RNAscope Fluorescent Multiplex kit (320293; Advanced Cell Diagnostics) was used with slight modification of manufacturer instructions and using commercially available probes [iCre (C2, #423321), Drd1a (C1, #317031), and D2 (C1, #315641)]."
Figure Legend
"Figure 9. Increased p-FAK expression in D1-MSN and D2-MSN, but p-cofilin only showed increased expression in D1-MSN during cue-induced cocaine seeking. p-FAK colocalization: A, D, Dendritic segments in NAcore labeled with mCherry-ChR2 (green, pseudo-colored images) in D1-MSN and D2-MSN and p-FAK (red) in each treatment group [saline (SAL), extinguished (EXT), reinstated (RST)]. Micrograph pairs of each segment depict the confocal imaging showing p-FAK puncta and labeled dendrites (left), and p-FAK puncta within the dendrite (right). Scale bar = 5 µm. B, Increased dendritic p-FAK immunoreactivity within D1-MSN dendrites in reinstated group compared with saline and extinguished animals. C, Positive correlation between p-FAK puncta expression within D1-MSN dendrite and active lever presses during 15-min cue-induced reinstatement. E, Increased dendritic p-FAK immunoreactivity within D2-MSN dendrites in reinstated group compared with saline and extinguished animals. F, No correlation between p-FAK puncta expression within the D2-MSN dendrite and active lever presses during cued reinstatement. p-cofilin colocalization: G, I, Dendritic segments in NAcore labeled with mCherry-ChR2 (green, pseudo-colored images) in D1-MSN and D2-MSN and p-cofilin (red) in each treatment group (SAL, EXT, RST). Micrograph pairs of each segment depict the confocal imaging showing p-cofilin puncta and labeled dendrites (left), and p-coflin puncta within the dendrite (right). Scale bar = 5 µm. H, Increased dendritic p-cofilin immunoreactivity within D1-MSN dendrites in reinstated group compared with saline and extinguished animals. J, No significant difference observed in p-cofilin puncta expression in D2-MSN dendrites. Data are shown as mean ± SEM N represents number of neurons quantified over number of animals in each condition; *p < 0.05 comparing RST to SAL; #p < 0.05 compared RST to EXT using Bonferroni post hoc. "
Protocol Conditions
Article Snippets for All Techniques