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tocris cat  (Tocris)


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    Structured Review

    Tocris tocris cat
    Tocris Cat, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cyclothiazide/Cyclothiazide/pm41936614-220-32-32
    Average 93 stars, based on 330 article reviews
    tocris cat - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    other:

    Article Title: Spinal α 2 δ -1 induces GluA3 degradation to regulate assembly of calcium-permeable AMPA receptors and pain hypersensitivity
    Article Snippet: Glutamate (10 mM; Sigma-Aldrich) and cyclothiazide (100 μM; Tocris Bioscience) were applied via a pressurized perfusion system (ALA Scientific Instruments) to elicit AMPAR currents.

    Article Title: Spinal α2δ-1 induces GluA3 degradation to regulate assembly of calcium-permeable AMPA receptors and pain hypersensitivity
    Article Snippet: Glutamate (10 mM, SigmaAldrich) and cyclothiazide (100 μM, Tocris Bioscience) were applied via a pressurized perfusion system (ALA Scientific Instruments, NY) to elicit AMPAR currents.

    Purification:

    Article Title: Structural basis for activation and conformational plasticity of the GluA4 AMPA receptor
    Article Snippet: Porcine Brain Polar Lipid Extract (Avanti, 141101 P) was tested for solubilizing receptors but was not used for the final purification (Supplementary Fig. ). .. Purified GluA4/TARPγ2 was treated with 100 μM cyclothiazide (CTZ, Tocris, 07-131-0) and ultracentrifuged (125,000 x g, 45 min at 4 °C) to remove precipitated protein prior to grid preparation. .. Au-Flat 1.2 μm/1.3 μm 300 mesh gold grids (MiTeGen, M-CEM-AUFT313) were plasma treated in a Pelco EasyGlow (25 mA, 120 s glow time and 10 s hold time; Ted Pella, 91000).

    Article Title: Structural basis for activation and conformational plasticity of the GluA4 AMPA receptor.
    Article Snippet: Porcine Brain Polar Lipid Extract (Avanti, 141101P) was tested for solubilizing receptors but was not used for the final purification (Supplementary Fig. 1b). .. AR TI CL E IN P RE SS Cryo-EM sample preparation and data collection Purified GluA4/TARPγ2 was treated with 100μM cyclothiazide (CTZ, Tocris, 07-131-0) and ultracentrifuged (125,000xg, 45 min at 4 oC) to remove precipitated protein prior to grid preparation. .. Au-Flat 1.2 μm/1.3 μm 300 mesh gold grids (MiTeGen, M-CEM-AUFT313) were plasma treated in a Pelco EasyGlow (25 mA, 120s glow time and 10s hold time; Ted Pella, 91000).

    Incubation:

    Article Title: Architecture, dynamics and biogenesis of GluA3 AMPA glutamate receptors.
    Article Snippet: The QuantiFoil 648 and UltraFoil grids were glow discharged at 25mA for 25 s and 90 s, respectively, using a 649 PELCO easiGlowTM to render them hydrophilic prior to vitrification. .. 650 651 For the open state, the protein was initially incubated with 300 μM cyclothiazide (Tocris, cat. 652 no. 0713), a positive allosteric modulator preventing desensitization, for at least 30 minutes on 653 ice, followed by rapid mixing with a 1 M L-glutamate stock solution (pH 7.4) to a final 654 concentration of 100 mM before grid application. .. 655 To induce the desensitized state for both GluA3-R and GluA3-G, 10 mM quisqualate (Tocris, 656 cat. no. 0188) was swiftly added to the protein to a final concentration of 1 mM before grid 657 application.

    Concentration Assay:

    Article Title: Architecture, dynamics and biogenesis of GluA3 AMPA glutamate receptors.
    Article Snippet: The QuantiFoil 648 and UltraFoil grids were glow discharged at 25mA for 25 s and 90 s, respectively, using a 649 PELCO easiGlowTM to render them hydrophilic prior to vitrification. .. 650 651 For the open state, the protein was initially incubated with 300 μM cyclothiazide (Tocris, cat. 652 no. 0713), a positive allosteric modulator preventing desensitization, for at least 30 minutes on 653 ice, followed by rapid mixing with a 1 M L-glutamate stock solution (pH 7.4) to a final 654 concentration of 100 mM before grid application. .. 655 To induce the desensitized state for both GluA3-R and GluA3-G, 10 mM quisqualate (Tocris, 656 cat. no. 0188) was swiftly added to the protein to a final concentration of 1 mM before grid 657 application.

    Cryo-EM Sample Prep:

    Article Title: Structural basis for activation and conformational plasticity of the GluA4 AMPA receptor.
    Article Snippet: Porcine Brain Polar Lipid Extract (Avanti, 141101P) was tested for solubilizing receptors but was not used for the final purification (Supplementary Fig. 1b). .. AR TI CL E IN P RE SS Cryo-EM sample preparation and data collection Purified GluA4/TARPγ2 was treated with 100μM cyclothiazide (CTZ, Tocris, 07-131-0) and ultracentrifuged (125,000xg, 45 min at 4 oC) to remove precipitated protein prior to grid preparation. .. Au-Flat 1.2 μm/1.3 μm 300 mesh gold grids (MiTeGen, M-CEM-AUFT313) were plasma treated in a Pelco EasyGlow (25 mA, 120s glow time and 10s hold time; Ted Pella, 91000).

    Sample Prep:

    Article Title: Structural basis for activation and conformational plasticity of the GluA4 AMPA receptor.
    Article Snippet: Porcine Brain Polar Lipid Extract (Avanti, 141101P) was tested for solubilizing receptors but was not used for the final purification (Supplementary Fig. 1b). .. AR TI CL E IN P RE SS Cryo-EM sample preparation and data collection Purified GluA4/TARPγ2 was treated with 100μM cyclothiazide (CTZ, Tocris, 07-131-0) and ultracentrifuged (125,000xg, 45 min at 4 oC) to remove precipitated protein prior to grid preparation. .. Au-Flat 1.2 μm/1.3 μm 300 mesh gold grids (MiTeGen, M-CEM-AUFT313) were plasma treated in a Pelco EasyGlow (25 mA, 120s glow time and 10s hold time; Ted Pella, 91000).



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