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Image Search Results
Journal: PLoS ONE
Article Title: Pharmacological characterisation of S 47445, a novel positive allosteric modulator of AMPA receptors
doi: 10.1371/journal.pone.0184429
Figure Lengend Snippet: EC 50 , EC 2X and maximal potentiation values obtained with S 47445 and other positive allosteric modulators at rat or human AMPA receptors on oocytes injected with poly(A+) mRNA.
Article Snippet:
Techniques: Injection
Journal: PLoS ONE
Article Title: Temperature effects on synaptic transmission and neuronal function in the visual thalamus
doi: 10.1371/journal.pone.0232451
Figure Lengend Snippet: A) Overlaid voltage-clamp recordings from a TC neuron from a Chx10-Cre;Ai32 mouse in response to pairs of 460 nm LED stimuli with intervals of 100, 200, 500, and 1000 ms (blue) at 23°C and 31°C in the presence of 200 μM γDGG and 100 μM cyclothiazide. Recordings were made in the presence of γDGG (200 μM) and cyclothiazide (100 μM). B) Plot of paired pulse ratio (PPR = EPSC2/EPSC1) showing that synaptic depression was increased following warming of the bath solution (n = 6). **p<0.01; ***p<0.001, t-test. C) Measurements of EPSC amplitude at room temperature and in warmed conditions (n = 8).
Article Snippet: For measurements of short-term synaptic plasticity and retinogeniculate synaptic vesicle release probability, the bath was supplemented with 200 μM γD-glutamylglycine (γDGG, Abcam, Cambridge, MA) and 100 μM
Techniques:
Journal: PLoS ONE
Article Title: Temperature effects on synaptic transmission and neuronal function in the visual thalamus
doi: 10.1371/journal.pone.0232451
Figure Lengend Snippet: A) Voltage-clamp recordings from a dLGN TC neuron EPSCs in response to a 10 Hz stimulation of retinal ganglion cell axons using a 460 nm LED in a brain slice from a Chx10-Cre;Ai32 mouse. Recordings were made in the presence of γDGG (200 μM) and cyclothiazide (100 μM). Each trace is the average of 3–5 individual responses to the same stimulus. B) Plot of cumulative EPSC amplitude and linear fit to the responses at pulses 15–30. Extrapolation to the Y-intercept is used as a measure of vesicle pool size while the slope is a measure of replenishment rate. Release probability is calculated as the ratio of the first EPSC to the Y-intercept. C) Vesicle release probability increased with warming the bath solution (n = 7 TC neuron recordings). D) Vesicle pool replenishment rate (slope of the fit in panel B) also increased with warming. E) The Y-intercept, which is a measure of vesicle pool size was not changed by warming.
Article Snippet: For measurements of short-term synaptic plasticity and retinogeniculate synaptic vesicle release probability, the bath was supplemented with 200 μM γD-glutamylglycine (γDGG, Abcam, Cambridge, MA) and 100 μM
Techniques: Slice Preparation