Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Development of VHL-recruiting STING PROTACs that suppress innate immunity

doi: 10.1007/s00018-023-04796-7

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Article Snippet: Cycloheximide , Selleck , cat# S6611.

Techniques: Transfection, Software, Reverse Transcription, SYBR Green Assay, Protease Inhibitor

Journal: eLife

Article Title: Auxiliary subunits keep AMPA receptors compact during activation and desensitization

doi: 10.7554/eLife.40548

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Article Snippet: Chemical compound, drug , Cyclothiazide; CTZ , Hello Bio, Bristol, UK , HB0221 , Obtained as powder, stock prepared in DMSO, used at final concentration of 100 μM..

Techniques: Transfection, Construct, Plasmid Preparation, Concentration Assay, Software

Journal: Cell reports

Article Title: Human amyotrophic lateral sclerosis excitability phenotype screen: Target discovery and validation

doi: 10.1016/j.celrep.2021.109224

Figure Lengend Snippet: (A) AMPA receptor subunit expression in SOD1(A4V) motor neurons and corrected isogenic controls (n = 4–5 samples, mean ± SD, two-tailed t test). (B) AMPA-induced calcium influx peak tested by fura-2 imaging (n = 50–70 neurons, mean ± SD, two-tailed t test, df = 135). (C) AMPA toxicity. AMPA (50 μM) and cyclothiazide (10 μM) were added to the culture for 48 h and then cells were fixed and stained with human nucleus (hN), MAP2, and the motor neuron marker Islet-1 (Isl1). The number of hN + Isl1 + nuclei, hN + MAP2 + neurons, and the total length of MAP2 + neurites (μm) per well were measured and normalized to untreated condition of each genotype (n = 12 wells, mean ± SD, two-tailed t test; scale bar, 100 μm). *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001. See also .

Article Snippet: Cyclothiazide , Cayman Chemical , Cat# 2259-9 6-3.

Techniques: Expressing, Two Tailed Test, Imaging, Staining, Marker

Journal: Cell reports

Article Title: Human amyotrophic lateral sclerosis excitability phenotype screen: Target discovery and validation

doi: 10.1016/j.celrep.2021.109224

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Cyclothiazide , Cayman Chemical , Cat# 2259-9 6-3.

Techniques: Virus, Recombinant, Drug discovery, Control, shRNA, Plasmid Preparation, Software