tubulin  (Proteintech)

Journal: FASEB BioAdvances

Article Title: Folic Acid Supplementation Attenuates Hepatic Steatosis by Enhancing Choline Availability and Remodeling Fatty Acid Profiles in Mice Fed a High‐Fat Diet

doi: 10.1096/fba.2025-00251

Figure Lengend Snippet: Relative mRNA expression levels of (A) phosphatidylethanolamine N‐methyltransferase ( Pemt ), (B) phospholipase D1 ( Pld1 ), (C) choline dehydrogenase ( Chdh ), (D) choline kinase a ( Chka ), and (E) choline‐phosphate cytidylyltransferase A ( Pcyt1a ) in the liver. Different letters indicate statistically significant differences between the means of groups by one‐way ANOVA with Tukey–Kramer post hoc test. Data presented as means ± SEM, n = 5–8/group.

Article Snippet: An equivalent volume of cDNA was then added to TaqMan Master Mix (Thermo Fisher Scientific) along with gene‐specific probes for phosphatidylethanolamine N‐methyltransferase ( Pemt ; Mm04933134_m1), phospholipase D1 ( Pld1 ; Mm01289339_m1), choline dehydrogenase ( Chdh ; Mm00549261_m1), choline kinase alpha ( Chka ; Mm00442759_m1), choline‐phosphate cytidylyltransferase 1 alpha ( Pcyt1a ; Mm00447774_m1), fatty acid synthase ( Fasn , Mm00662319_m1), stearoyl‐CoA desaturase 1 ( Scd1 , Mm00772290_m1), peroxisome proliferator‐activated receptor alpha ( Ppara , Mm00440939_m1), and carnitine palmitoyltransferase 1A ( Cpt1a , Mm01231183_m1), which were run on a QuantStudio 5 Real‐Time PCR System (Thermo Fisher Scientific).

Techniques: Expressing

Journal: Fluids and barriers of the CNS

Article Title: Investigating post-traumatic syringomyelia and local fluid osmoregulation via a rat model.

doi: 10.1186/s12987-024-00514-y

Figure Lengend Snippet: Fig. 4 Expression of betaine regulating factors in PTSM environment. Region-specific expression of A. CHDH (red) and B. BGT1 (red) in different segments of the spinal cord of PTSM injured animals (n = 3) showing PTSM rostral, PTSM injury, PTSM caudal, compared to laminectomy-only control animals (n = 3), co-stained with GFAP, astrocyte marker (green). In all IHC image panels DAPI (blue), scale bar = 80 µm, and * = syrinx

Article Snippet: Staining was performed for target proteins/channels/ enzymes such as BGT1 (betaine-GABA transporter 1) (Anti-BGT1 (SLC6A12) antibody, Alomone Laboratories, Israel), CHDH (choline dehydrogenase) (sc393885, Santa Cruz Biotechnology, TX, USA), AQP1 (sc-25287, Santa Cruz Biotechnology, TX, USA), AQP4 (sc-32739, Santa Cruz Biotechnology, TX, USA), and KCC4 (potassium-chloride cotransporter 4) (PA5-59666, ThermoFisher, MA, USA) using primary and secondary antibodies.

Techniques: Expressing, Control, Staining, Marker

Journal: Aging (Albany NY)

Article Title: Mitochondrial-related genes PDK2, CHDH, and ALDH5A1 served as a diagnostic signature and correlated with immune cell infiltration in ulcerative colitis

doi: 10.18632/aging.205561

Figure Lengend Snippet: Analysis of immune infiltration. ( A ) Bar plot bar plot depicting the 22 subpopulations of immune cells in UC and healthy control (HC) samples. ( B ) Correlation heatmap of immune cells in UC samples. ( C ) Violin plot illustrating the varying proportions of immune cells between UC and normal control (NC). ( D – F ) Bubble plots demonstrate the relationship between immune cells and specific signature genes (PDK2, CHDH, and ALDH5A1).

Article Snippet: The membranes were then incubated overnight at 4°C with the following antibodies: ALDH5A1 polyclonal antibody (17319-1-AP), CHDH polyclonal antibody (17356-1-AP), PDK2 polyclonal antibody (15647-1-AP), and GAPDH (60004-1-Ig) from ProteinTech group (Wuhan, China).

Techniques: Control

Journal: Aging (Albany NY)

Article Title: Mitochondrial-related genes PDK2, CHDH, and ALDH5A1 served as a diagnostic signature and correlated with immune cell infiltration in ulcerative colitis

doi: 10.18632/aging.205561

Figure Lengend Snippet: Expression of PDK2, CHDH, and ALDH5A1 in LPS-induced HIEC-6 cells and DSS-induced colitis mice. The relative mRNA ( A ) and protein ( B ) expression levels of PDK2, CHDH, and ALDH5A1 were conducted using the LPS-induced HIEC-6 cells. ( C ) Photographs were utilized to assess the variations in colon lengths among the groups. ( D ) The length of the colon in different groups. ( E , F ) Hematoxylin and eosin staining images and colonic histological scores of colon cross-sections in mice ( n = 5). Scale bar = 50 μm. ( G ) Expression of PDK2, CHDH, and ALDH5A1 in the colon tissues of mice was detected using Western blotting. ( H ) Double immunofluorescence staining of PDK2 (labeled in green), CHDH (labeled in green), and ALDH5A1 (labeled in green) with M1 macrophages (CD86, labeled in red) at DSS induced mice. The nuclei were stained by DAPI (blue). n = 5 per group. Scale bar = 50 μm. Values are expressed as the mean ± sd ( n = 5). * P < 0.05, ** P < 0.01, *** P < 0.001. Abbreviation: DSS: dextran sodium sulfate.

Article Snippet: The membranes were then incubated overnight at 4°C with the following antibodies: ALDH5A1 polyclonal antibody (17319-1-AP), CHDH polyclonal antibody (17356-1-AP), PDK2 polyclonal antibody (15647-1-AP), and GAPDH (60004-1-Ig) from ProteinTech group (Wuhan, China).

Techniques: Expressing, Staining, Western Blot, Double Immunofluorescence Staining, Labeling

Journal: Aging (Albany NY)

Article Title: Mitochondrial-related genes PDK2, CHDH, and ALDH5A1 served as a diagnostic signature and correlated with immune cell infiltration in ulcerative colitis

doi: 10.18632/aging.205561

Figure Lengend Snippet: Primer sequences.

Article Snippet: The membranes were then incubated overnight at 4°C with the following antibodies: ALDH5A1 polyclonal antibody (17319-1-AP), CHDH polyclonal antibody (17356-1-AP), PDK2 polyclonal antibody (15647-1-AP), and GAPDH (60004-1-Ig) from ProteinTech group (Wuhan, China).

Techniques: