Journal: Translational Oncology

Article Title: Quantitative proteomic profiling of hepatocellular carcinoma at different serum alpha-fetoprotein level

doi: 10.1016/j.tranon.2022.101422

Figure Lengend Snippet: Identification of potential drug targets in HBV-related HCC. PPI network of differentially expressed proteins in tumor (A) and adjacent noncancerous tissues (B) at cut-off value of 400 ng/mL for AFP with node size representing fold changes. Hub proteins of the PPI by Mcode plugin in tumor (C) and adjacent noncancerous tissues (D). Predicted three-dimensional structures of CHDH (E), ITGAL (F) in adjacent noncancerous tissues and C1QBP (G), HSPE1 (H), GLUD2 (I) in tumor tissues predicted by AlphaFold. AFP, alpha-fetoprotein; HBV, hepatitis B virus; HCC, Hepatocellular carcinoma; PPI, protein-protein interaction.

Article Snippet: The primary antibodies included C1QBP (1:1000, Cell Signaling TECHNOLOGY, 6502S), GLUD2 (1:600, Proteintech, 14462-1-AP), HSPE1 (1:10000, SANTA CRUZ BIOTECHNOLOGY, sc-376313), CHDH (1:900, Proteintech, 17356-1-AP) and ITGAL (1:200, Proteintech, 15574-1-AP).

Techniques: Virus

Journal: Translational Oncology

Article Title: Quantitative proteomic profiling of hepatocellular carcinoma at different serum alpha-fetoprotein level

doi: 10.1016/j.tranon.2022.101422

Figure Lengend Snippet: Differential expression levels of five identified hub proteins by immunohistochemistry. The upper and lower in left panel were magnified by 5x and 20x, separately. The left and right in left panel were representative immunohistochemistry results of tumor and adjacent noncancerous tissues. The right panels displayed comparison results of relative immunohistochemistry scores between tumor and adjacent noncancerous tissues in HCC tissue microarrays at protein level for C1QBP (A), CHDH (B), GLUD2(C), ITGAL (D) and HSPE1 (E), separately. Scale bar, 100 μm. Wilcoxon rank sum test was used for comparison statistics. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** p < 0.0001. P < 0.05 was considered statistical significant. Scale bar 100 µm. HCC, Hepatocellular carcinoma; Immunohistochemistry, IHC.

Article Snippet: The primary antibodies included C1QBP (1:1000, Cell Signaling TECHNOLOGY, 6502S), GLUD2 (1:600, Proteintech, 14462-1-AP), HSPE1 (1:10000, SANTA CRUZ BIOTECHNOLOGY, sc-376313), CHDH (1:900, Proteintech, 17356-1-AP) and ITGAL (1:200, Proteintech, 15574-1-AP).

Techniques: Quantitative Proteomics, Immunohistochemistry, Comparison

Journal: Lipids in Health and Disease

Article Title: Tetradecylthiopropionic acid induces hepatic mitochondrial dysfunction and steatosis, accompanied by increased plasma homocysteine in mice

doi: 10.1186/s12944-016-0192-9

Figure Lengend Snippet: Metabolites of the homocysteine remethylation and sulfoxation, transsulfuration, and choline oxidation pathways. Names of enzymes are beside the arrows. Vitamins involved in the enzymatic reactions are shown in green circles. Abbreviations: BADH, betaine aldehyde dehydrogenase; BHMT, betaine-homocysteine methyltransferase; CBS, cystathionine beta-synthase; CHDH, choline dehydrogenase; CTH, cystathionine gamma-lyase; DMG, dimethylglycine; DMGDH, dimethylglycine dehydrogenase; GNMT, glycine N-methyltransferase; Hcy, homocysteine; MAT, methionine adenosyltransferase; Met, methionine; MetSO, methionine sulfoxide; 5-mTHF, 5-methyltetrahydrofolate; MTs, methyltransferases; MTR, methionine synthase; MSR (encoded by the gene Mtrr ), methionine synthase reductase; PC, phosphatidylcholine; PE, phosphatidyletanolamine; PEMT, phosphatidyl-ethanolamine methyltransferase; SAH, s-adenosylhomocysteine; SAM, s-adenosylmethionine; SARDH, sarcosine dehydrogenase; SHMT1, serine hydroxymethyltransferase 1; SHMT2, serine hydroxymethyltransferase 2; THF, tetrahydrofolate; TML, trimethyllysine

Article Snippet: Real-time PCR was performed with Sarstedt 384 well multiply-PCR Plates (Sarstedt Inc., Newton, NC, USA) on the following genes, using probes and primers from Applied Biosystems: Chka (Mm00442759_m1), Chkb (Mm04213225_s1), Chdh (Mm00549261_m1), Bhmt (Mm04210521_g1), Dmgdh (Mm00512022_m1), Sardh (Mm00454657_m1), Shmt1 (Mm00486110_m1), Shmt2 (Mm00659512_g1), Gnmt (Mm00494688_m1), Mtr (Mm01340053_m1), Mtrr _(Mm00549978_m1), Msra (Mm00452738_m1), Msrb2 (Mm00512937_m1), Cbs (Mm00460654_m1), Cth (Mm00461247_m1), Ppargc1a (Mm00447183), Pparg (Mm00440945), Ppara (Mm00440939), CD36 / Fat (Mm00432403), Fabp1 (Mm00444340), Lipe (Mm00495359), Acly (Mm01302282_m1), Slc25a20 (Mm00451571_m1), Lipc (Mm00433975), Apob (Mm01545156_m1), IL1β (Mm01336189_m1), Sod1 (Mm01344233_m1), Sod2 (Mm01313000_m1), Tnfα (Mm00443258_m1) and Pcyt1a (Mm00447774_m1).

Techniques:

Journal: Lipids in Health and Disease

Article Title: Tetradecylthiopropionic acid induces hepatic mitochondrial dysfunction and steatosis, accompanied by increased plasma homocysteine in mice

doi: 10.1186/s12944-016-0192-9

Figure Lengend Snippet: Hepatic gene expression levels a

Article Snippet: Real-time PCR was performed with Sarstedt 384 well multiply-PCR Plates (Sarstedt Inc., Newton, NC, USA) on the following genes, using probes and primers from Applied Biosystems: Chka (Mm00442759_m1), Chkb (Mm04213225_s1), Chdh (Mm00549261_m1), Bhmt (Mm04210521_g1), Dmgdh (Mm00512022_m1), Sardh (Mm00454657_m1), Shmt1 (Mm00486110_m1), Shmt2 (Mm00659512_g1), Gnmt (Mm00494688_m1), Mtr (Mm01340053_m1), Mtrr _(Mm00549978_m1), Msra (Mm00452738_m1), Msrb2 (Mm00512937_m1), Cbs (Mm00460654_m1), Cth (Mm00461247_m1), Ppargc1a (Mm00447183), Pparg (Mm00440945), Ppara (Mm00440939), CD36 / Fat (Mm00432403), Fabp1 (Mm00444340), Lipe (Mm00495359), Acly (Mm01302282_m1), Slc25a20 (Mm00451571_m1), Lipc (Mm00433975), Apob (Mm01545156_m1), IL1β (Mm01336189_m1), Sod1 (Mm01344233_m1), Sod2 (Mm01313000_m1), Tnfα (Mm00443258_m1) and Pcyt1a (Mm00447774_m1).

Techniques: Gene Expression, Control, Binding Assay, Antioxidant Activity Assay