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stat3(s727) (4g1) monoclonal antibody  (Bioss)


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    Bioss stat3(s727) (4g1) monoclonal antibody
    Stat3(S727) (4g1) Monoclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stat3(s727) (4g1) monoclonal antibody/product/Bioss
    Average 94 stars, based on 4 article reviews
    stat3(s727) (4g1) monoclonal antibody - by Bioz Stars, 2026-02
    94/100 stars

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    The phosphorylation of STAT3 was crucial for the IL-22-mediated production of TRIM25. A DEFs cells were stimulated with different amounts of IL-22 and WP1066 and were harvested 24 h post-treatment. Then, 30 mg of protein lysates were analyzed for P-STAT3 (S727) and total STAT3 via Western blot analysis (anti-pSTAT3 1:3000, anti-STAT3 1:3000, anti-β-actin 1:4000, HRP-conjugated goat anti-mouse antibody 1:3000). B The inhibition of P-STAT3 suppressed TRIM25 production. DEFs were treated with 5 µM WP1066 (or PBS in the control group) and IL-22 at 50 ng/mL for 24 h; TRIM25 expression was then measured via qRT-PCR and Western blotting. The results are expressed as the mean ± SEM of three independent experiments. * P < 0.05, *** P < 0.001.

    Journal: Veterinary Research

    Article Title: Interleukin-22 facilitates the interferon-λ-mediated production of tripartite motif protein 25 to inhibit replication of duck viral hepatitis A virus type 1

    doi: 10.1186/s13567-023-01188-4

    Figure Lengend Snippet: The phosphorylation of STAT3 was crucial for the IL-22-mediated production of TRIM25. A DEFs cells were stimulated with different amounts of IL-22 and WP1066 and were harvested 24 h post-treatment. Then, 30 mg of protein lysates were analyzed for P-STAT3 (S727) and total STAT3 via Western blot analysis (anti-pSTAT3 1:3000, anti-STAT3 1:3000, anti-β-actin 1:4000, HRP-conjugated goat anti-mouse antibody 1:3000). B The inhibition of P-STAT3 suppressed TRIM25 production. DEFs were treated with 5 µM WP1066 (or PBS in the control group) and IL-22 at 50 ng/mL for 24 h; TRIM25 expression was then measured via qRT-PCR and Western blotting. The results are expressed as the mean ± SEM of three independent experiments. * P < 0.05, *** P < 0.001.

    Article Snippet: Horseradish peroxidase (HRP)-conjugated goat anti-mouse antibody (bs-0774R-HRP), HRP-conjugated goat anti-rabbit antibody (bs-40295G-HRP), rabbit anti-STAT3 antibody (bs-1141R), and rabbit anti-phospho-STAT3 (Ser727) antibody (bsm-52210R) were purchased from Bioss antibodies (Beijing, China).

    Techniques: Western Blot, Inhibition, Control, Expressing, Quantitative RT-PCR

    HP inhibits the JAK1/STAT3 signaling pathway, but has no effect on the NF-κB signaling pathway in LPS-treated BV-2 microglial cells. Cells were pre-treated with 20 µg/ml HP for 30 min, followed by treatment with LPS (1 µg/ml) for the indicated durations. (A) Expression levels of p-JAK1, JAK1, p-STAT3 and STAT3 proteins were detected by western blotting. (B and C) The associated protein expression levels are presented as the means ± SD, * P<0.05, ** P<0.01 and *** P<0.001. (D) The expression of IκB-α protein was detected by western blotting. (E) The associated protein expression levels are presented as means ± SD. HP, hispidin; LPS, lipopolysaccharide; p, phosphorylated.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Hispidin inhibits LPS-induced nitric oxide production in BV-2 microglial cells via ROS-dependent MAPK signaling

    doi: 10.3892/etm.2021.10402

    Figure Lengend Snippet: HP inhibits the JAK1/STAT3 signaling pathway, but has no effect on the NF-κB signaling pathway in LPS-treated BV-2 microglial cells. Cells were pre-treated with 20 µg/ml HP for 30 min, followed by treatment with LPS (1 µg/ml) for the indicated durations. (A) Expression levels of p-JAK1, JAK1, p-STAT3 and STAT3 proteins were detected by western blotting. (B and C) The associated protein expression levels are presented as the means ± SD, * P<0.05, ** P<0.01 and *** P<0.001. (D) The expression of IκB-α protein was detected by western blotting. (E) The associated protein expression levels are presented as means ± SD. HP, hispidin; LPS, lipopolysaccharide; p, phosphorylated.

    Article Snippet: Rabbit monoclonal antibodies for phosphorylated (p)-STAT3 (cat. no. bsm-52210R, dilution: 1:1,000), STAT3 (cat. no. bsm-52235R, dilution: 1:500), p-JNK (cat. no. bsm-52462R, dilution: 1:1,000), IκB-α (cat. no. bsm-52169R, dilution: 1:500) and rabbit polyclonal antibodies for p-JAK1 (cat. no. bs-3238R dilution: 1:1,000), JAK1 (cat. no. bs-1439R, dilution: 1:1,000), p-ERK (cat. no. bs-3238R, dilution: 1:1,000), JNK (cat. no. bs-10562R, dilution: 1:1,000), p-P38 (cat. no. bs-5477R, dilution: 1:1,000) and P38 (cat. no. bs-0637R, dilution: 1:1,000) were purchased from BIOSS.

    Techniques: Expressing, Western Blot