bsm-52210R Search Results


phosphorylated p stat3  (Bioss)
94
Bioss phosphorylated p stat3
HP inhibits the <t>JAK1/STAT3</t> signaling pathway, but has no effect on the NF-κB signaling pathway in LPS-treated BV-2 microglial cells. Cells were pre-treated with 20 µg/ml HP for 30 min, followed by treatment with LPS (1 µg/ml) for the indicated durations. (A) Expression levels of p-JAK1, JAK1, <t>p-STAT3</t> and STAT3 proteins were detected by western blotting. (B and C) The associated protein expression levels are presented as the means ± SD, * P<0.05, ** P<0.01 and *** P<0.001. (D) The expression of IκB-α protein was detected by western blotting. (E) The associated protein expression levels are presented as means ± SD. HP, hispidin; LPS, lipopolysaccharide; p, phosphorylated.
Phosphorylated P Stat3, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphorylated p stat3 - by Bioz Stars, 2026-02
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HP inhibits the JAK1/STAT3 signaling pathway, but has no effect on the NF-κB signaling pathway in LPS-treated BV-2 microglial cells. Cells were pre-treated with 20 µg/ml HP for 30 min, followed by treatment with LPS (1 µg/ml) for the indicated durations. (A) Expression levels of p-JAK1, JAK1, p-STAT3 and STAT3 proteins were detected by western blotting. (B and C) The associated protein expression levels are presented as the means ± SD, * P<0.05, ** P<0.01 and *** P<0.001. (D) The expression of IκB-α protein was detected by western blotting. (E) The associated protein expression levels are presented as means ± SD. HP, hispidin; LPS, lipopolysaccharide; p, phosphorylated.

Journal: Experimental and Therapeutic Medicine

Article Title: Hispidin inhibits LPS-induced nitric oxide production in BV-2 microglial cells via ROS-dependent MAPK signaling

doi: 10.3892/etm.2021.10402

Figure Lengend Snippet: HP inhibits the JAK1/STAT3 signaling pathway, but has no effect on the NF-κB signaling pathway in LPS-treated BV-2 microglial cells. Cells were pre-treated with 20 µg/ml HP for 30 min, followed by treatment with LPS (1 µg/ml) for the indicated durations. (A) Expression levels of p-JAK1, JAK1, p-STAT3 and STAT3 proteins were detected by western blotting. (B and C) The associated protein expression levels are presented as the means ± SD, * P<0.05, ** P<0.01 and *** P<0.001. (D) The expression of IκB-α protein was detected by western blotting. (E) The associated protein expression levels are presented as means ± SD. HP, hispidin; LPS, lipopolysaccharide; p, phosphorylated.

Article Snippet: Rabbit monoclonal antibodies for phosphorylated (p)-STAT3 (cat. no. bsm-52210R, dilution: 1:1,000), STAT3 (cat. no. bsm-52235R, dilution: 1:500), p-JNK (cat. no. bsm-52462R, dilution: 1:1,000), IκB-α (cat. no. bsm-52169R, dilution: 1:500) and rabbit polyclonal antibodies for p-JAK1 (cat. no. bs-3238R dilution: 1:1,000), JAK1 (cat. no. bs-1439R, dilution: 1:1,000), p-ERK (cat. no. bs-3238R, dilution: 1:1,000), JNK (cat. no. bs-10562R, dilution: 1:1,000), p-P38 (cat. no. bs-5477R, dilution: 1:1,000) and P38 (cat. no. bs-0637R, dilution: 1:1,000) were purchased from BIOSS.

Techniques: Expressing, Western Blot