Journal: Journal of Cellular and Molecular Medicine
Article Title: Lycorine ( Lycoris radiata )—a unique natural medicine on breast cancer
doi: 10.1111/jcmm.70032
Figure Lengend Snippet: Expression of proteins in cell cycle arrest. (A) Expression of protein CyclinB1, CDK1, p21 by western blot. (B) Expression of protein Aurora A and Aurora B by western blot. (C, D, E and F) Quantification of the p‐H3, PLK1, BRCA1 and BUBR1 protein in cells by FITC staining with flow cytometry. (G)Expression of protein STAT3, PAK1, CAMK4, PKA and ERK by western blot. (H) PCA ordination plot for eigenvalue decomposition covariance matrixes among cell cycle arrest relative proteins based on the relative abundance. Significant differences were indicated as * p < 0.05, ** p < 0.01 and *** p < 0.001 compared with the control group.
Article Snippet: Primary antibodies targeting CyclinB1, CDK1, p21, Aurora A, Aurora B, p‐H3, PLK1, BRCA1, BUBR1, STAT3, PAK1, CAMK4, PKA, ERK1/2, GAPDH and β‐actin were purchased from Bioss Biotechnology (Beijing, China).
Techniques: Expressing, Western Blot, Staining, Flow Cytometry, Control