Journal: Frontiers in Cell and Developmental Biology

Article Title: Comprehensive bioinformatics and in vitro studies reveal the carcinogenic role and molecular basis of endocrine disruptors in prostate cancer

doi: 10.3389/fcell.2025.1712195

Figure Lengend Snippet: Survival analysis of core genes (CD38, MMP11, and PLK1) in three cohorts. (A–C) CD38; (D–F) MMP11; (G–I) PLK1; (A,D,G) TCGA-PRAD; (B,E,H) GSE; (C,F,I) GSE116918 . Note: H: Expressing an array higher than the median; L: Expressing an array lower than the median.

Article Snippet: The membranes were blocked with 5% skim milk at room temperature for 1 h, followed by incubation with an anti-PLK1 primary antibody (1:1,000 dilution; catalog #10305-1-AP, Proteintech) at 4 °C overnight.

Techniques: Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Comprehensive bioinformatics and in vitro studies reveal the carcinogenic role and molecular basis of endocrine disruptors in prostate cancer

doi: 10.3389/fcell.2025.1712195

Figure Lengend Snippet: The expression and localization of core genes in the tumor microenvironment. (A) 7 single-cell subpopulations; (B) Annotation markers for 7 single-cell subpopulations; (C) The expression of 9 core genes in the microenvironment; (D) Differential expression of core gene PLK1 in 26 TCGA database tumors. Note: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Article Snippet: The membranes were blocked with 5% skim milk at room temperature for 1 h, followed by incubation with an anti-PLK1 primary antibody (1:1,000 dilution; catalog #10305-1-AP, Proteintech) at 4 °C overnight.

Techniques: Expressing, Quantitative Proteomics

Journal: Materials Today Bio

Article Title: Targeted biomimetic fusogenic liposome enhances tumor accumulation, penetration, and therapeutic efficacy of siRNA therapeutics

doi: 10.1016/j.mtbio.2025.102414

Figure Lengend Snippet: Antitumor effects of CRPIL/siCD47/siPLK1 complexes. (a). Expression of CD4 7 mRNA and PLK1 mRNA in 4T1 cells after different treatments. (b) The phagocytosis efficiency of J774A.1 cells against 4T1 cells after different treatments. (c) Schematic illustration of treatment schedule. (d) Changes in tumor volumes during the treatment. (e–f) Photographs of dissected tumors and average tumor weights at the end of the treatment. (g) Expression of CD47 and PLK1 proteins in tumor tissues after different treatments. (h) Representative images of hematoxylin and eosin-stained, TUNEL-stained and calreticulin (CRT)-stained tumor tissues on day 14.

Article Snippet: After washing, the membranes were incubated with CD47 Polyclonal antibody (Proteintech Group, 20305-1-AP), PLK1 Polyclonal antibody (Proteintech Group, 10305-1-AP) or GAPDH Monoclonal antibody (Proteintech Group, 60004-1-Ig) for 1 h at room temperature.

Techniques: Expressing, Staining, TUNEL Assay

Journal: Biofactors (Oxford, England)

Article Title: ASNS Regulates H 2 O 2 ‐Induced Senescence, Oxidative Stress, and Glucose Metabolism in ARPE ‐19 Cells by Modulating USP13 Expression

doi: 10.1002/biof.70057

Figure Lengend Snippet: Analysis of ASNS interaction with downstream proteins. Binding of ASNS to GSK3B (A), ITCH (B), PLK1 (C), USP13 (D), USP22 (E) and WDR4 (F) proteins was analyzed by CO‐IP in ARPE‐19 cells.

Article Snippet: , PLK1 , 10,305‐1‐AP , Proteintech , 1:1000.

Techniques: Binding Assay, Co-Immunoprecipitation Assay