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rabbit anti sparcl1 (Bioss)

Name: rabbit anti sparcl1
Brand: Bioss
Rating: 94 (4 reviews)

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Bioss rabbit anti sparcl1
Rabbit Anti Sparcl1, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti sparcl1/product/Bioss
Average 94 stars, based on 4 article reviews

rabbit anti sparcl1 - by Bioz Stars, 2026-02

94/100 stars

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Fig. 1 SPARCL1 inﬂuences C2C12 cell differentiation. a, e shows the expression of SPARCL1 protein activated or inhibited in C2C12 cells when the cells were induced to differentiate at 72 h. pSPgRNA-S-2 is the SPARCL1 activation group and pSPgRNA is the blank control for SPARCL1 activation. NC was the negative control for SPARCL1 siRNA interference. b–d are grayscale scans of the proteins shown in a. f–h are grayscale scans of the proteins shown in e. i, k show Desmin expression in C2C12 cells when SPARCL1 was activated or inhibited at 72 h. j, l shows the quantiﬁcation of myotubes according to the Desmin staining of I and K. The scale bar in I and K is 100 μm; the green ﬂuorescent signal is Desmin, while the blue ﬂuorescent signal is the nucleus. **P < 0.01 were considered as signiﬁcant

Journal: Cell death & disease

Article Title: SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway.

doi: 10.1038/s41419-019-2049-4

Figure Lengend Snippet: Fig. 1 SPARCL1 inﬂuences C2C12 cell differentiation. a, e shows the expression of SPARCL1 protein activated or inhibited in C2C12 cells when the cells were induced to differentiate at 72 h. pSPgRNA-S-2 is the SPARCL1 activation group and pSPgRNA is the blank control for SPARCL1 activation. NC was the negative control for SPARCL1 siRNA interference. b–d are grayscale scans of the proteins shown in a. f–h are grayscale scans of the proteins shown in e. i, k show Desmin expression in C2C12 cells when SPARCL1 was activated or inhibited at 72 h. j, l shows the quantiﬁcation of myotubes according to the Desmin staining of I and K. The scale bar in I and K is 100 μm; the green ﬂuorescent signal is Desmin, while the blue ﬂuorescent signal is the nucleus. **P < 0.01 were considered as signiﬁcant

Article Snippet: The membrane was blocked with 5% skimmed milk/PBST for 60min at 37 °C, and then the membranes were incubated with primary antibodies (SPARCL1 (catalog number: bs-6110R), BMP7 (catalog number: bs-2242R), MYOG (catalog number: bs-3550R), GAPDH (catalog number: bs-0755R), SMAD4 (catalog number: bs23966R), Desmin (catalog number: bs-20702R), all from Bioss and P-SMAD4 (catalog number: AF8316) from Affinty USA) overnight at 4 °C.

Techniques: Cell Differentiation, Expressing, Activation Assay, Control, Negative Control, Staining

Fig. 2 SPARCL1 expressed in mice muscle injury model. a shows HE staining of TA muscles injury. b shows the Protein expression changes of SPARCL1 in muscle damage repair, MyoD, MHC, Pax7+ are marker molecules involved in cell differentiation, c–f are grayscale scans of the relevant proteins in b. g shows the SPARCL1 expressed in mouse muscle injury model. The green ﬂuorescent signal is SPARCL1, red ﬂuorescent signal is laminin, and blue ﬂuorescent signal is cell nucleus. H shows statistical data for positive cell number based on SPARCL1 expression in 2G. Five ﬁelds of view per experimental group were used to analyse SPARCL1 expression in TA muscle cells. D0, D1, D3, D5, and D7 represent TA muscle damage on days 0, 1, 3, 5, and 7, respectively. The scale bar in A and B is 100 μm. **P values < 0.01 and *P values < 0.05 were considered as signiﬁcant

Journal: Cell death & disease

Article Title: SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway.

doi: 10.1038/s41419-019-2049-4

Figure Lengend Snippet: Fig. 2 SPARCL1 expressed in mice muscle injury model. a shows HE staining of TA muscles injury. b shows the Protein expression changes of SPARCL1 in muscle damage repair, MyoD, MHC, Pax7+ are marker molecules involved in cell differentiation, c–f are grayscale scans of the relevant proteins in b. g shows the SPARCL1 expressed in mouse muscle injury model. The green ﬂuorescent signal is SPARCL1, red ﬂuorescent signal is laminin, and blue ﬂuorescent signal is cell nucleus. H shows statistical data for positive cell number based on SPARCL1 expression in 2G. Five ﬁelds of view per experimental group were used to analyse SPARCL1 expression in TA muscle cells. D0, D1, D3, D5, and D7 represent TA muscle damage on days 0, 1, 3, 5, and 7, respectively. The scale bar in A and B is 100 μm. **P values < 0.01 and *P values < 0.05 were considered as signiﬁcant

Techniques: Staining, Muscles, Expressing, Marker, Cell Differentiation

Fig. 3 SPARCL1 interacted with BMP7. a shows the Co-IP results of SPARCL1 SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β protein interacting with BMP7 protein. b shows the Co-IP results of BMP7 protein interacting with SPARCL1 protein. In a, b, Input indicates the positive control group, IgG indicates the negative control group, IP indicates the target experimental group, and IB indicates the veriﬁcation of SPARCL1 and BMP7 in a and b, respectively

Journal: Cell death & disease

Article Title: SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway.

doi: 10.1038/s41419-019-2049-4

Figure Lengend Snippet: Fig. 3 SPARCL1 interacted with BMP7. a shows the Co-IP results of SPARCL1 SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β protein interacting with BMP7 protein. b shows the Co-IP results of BMP7 protein interacting with SPARCL1 protein. In a, b, Input indicates the positive control group, IgG indicates the negative control group, IP indicates the target experimental group, and IB indicates the veriﬁcation of SPARCL1 and BMP7 in a and b, respectively

Techniques: Co-Immunoprecipitation Assay, Cell Differentiation, Positive Control, Negative Control

Fig. 5 BMP7 inﬂuences C2C12 cells differentiation. a, e show the expression of BMP7 protein activated or inhibited in C2C12 cells when the cells were induced to differentiate at 72 h. pSPgRNA-B-3 was the BMP7 activation group, pSPgRNA was the blank control for BMP7 activation. NC was a negative control for BMP7 siRNA interference. b–d shows greyscale scans of the proteins in A. f–h shows greyscale scans of the proteins in e. i, k shows Desmin expression in C2C12 cells when SPARCL1 was activated or inhibited at 72 h. j, l shows quantiﬁcation of myotubes according to Desmin staining in i, k, respectively. The SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β scale bar in i, k is 100 μm, and the green ﬂuorescent signal is Desmin, while the blue ﬂuorescent signal is the nucleus. **P values < 0.01 and *P values < 0.05 were considered as signiﬁcant

Journal: Cell death & disease

Article Title: SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway.

doi: 10.1038/s41419-019-2049-4

Figure Lengend Snippet: Fig. 5 BMP7 inﬂuences C2C12 cells differentiation. a, e show the expression of BMP7 protein activated or inhibited in C2C12 cells when the cells were induced to differentiate at 72 h. pSPgRNA-B-3 was the BMP7 activation group, pSPgRNA was the blank control for BMP7 activation. NC was a negative control for BMP7 siRNA interference. b–d shows greyscale scans of the proteins in A. f–h shows greyscale scans of the proteins in e. i, k shows Desmin expression in C2C12 cells when SPARCL1 was activated or inhibited at 72 h. j, l shows quantiﬁcation of myotubes according to Desmin staining in i, k, respectively. The SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β scale bar in i, k is 100 μm, and the green ﬂuorescent signal is Desmin, while the blue ﬂuorescent signal is the nucleus. **P values < 0.01 and *P values < 0.05 were considered as signiﬁcant

Techniques: Expressing, Activation Assay, Control, Negative Control, Staining, Cell Differentiation

Fig. 6 SPARCL1 regulates BMP7 expression and BMP/TGF-β cell signaling pathway. a, d shows the protein expression of BMP7 regulated by SPARCL1 activation and inhibition, respectively. C2C12 cells were induced to differentiate at 72 h. pSPgRNA-S-2 is the SPARCL1 activation group, while pSPgRNA is the blank control for SPARCL1 activation. NC is the negative control for SPARCL1 siRNA interference. b, c are greyscale scans of SPARCL1 and BMP7 proteins in A. e, f are grayscale scans of SPARCL1 and BMP7 proteins in D. i and p shows changes in the expression of BMP/ TGF-β-associated proteins when SPARCL1 was activated and inhibited, respectively, and C2C12 cells were induced to differentiate at 72 h. pSPgRNA-S-2 is the SPARCL1 activation group, while pSPgRNA is the blank control for SPARCL1 activation. NC is the negative control for SPARCL1 siRNA interference. j–o are greyscale scans of proteins in I. q–v are greyscale scans of proteins in P. **P values < 0.01 were considered as signiﬁcant

Journal: Cell death & disease

Article Title: SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway.

doi: 10.1038/s41419-019-2049-4

Figure Lengend Snippet: Fig. 6 SPARCL1 regulates BMP7 expression and BMP/TGF-β cell signaling pathway. a, d shows the protein expression of BMP7 regulated by SPARCL1 activation and inhibition, respectively. C2C12 cells were induced to differentiate at 72 h. pSPgRNA-S-2 is the SPARCL1 activation group, while pSPgRNA is the blank control for SPARCL1 activation. NC is the negative control for SPARCL1 siRNA interference. b, c are greyscale scans of SPARCL1 and BMP7 proteins in A. e, f are grayscale scans of SPARCL1 and BMP7 proteins in D. i and p shows changes in the expression of BMP/ TGF-β-associated proteins when SPARCL1 was activated and inhibited, respectively, and C2C12 cells were induced to differentiate at 72 h. pSPgRNA-S-2 is the SPARCL1 activation group, while pSPgRNA is the blank control for SPARCL1 activation. NC is the negative control for SPARCL1 siRNA interference. j–o are greyscale scans of proteins in I. q–v are greyscale scans of proteins in P. **P values < 0.01 were considered as signiﬁcant

Techniques: Expressing, Activation Assay, Inhibition, Control, Negative Control

Fig. 7 SPARCL1 regulates C2C12 cell differentiation through BMP/TGF-β signaling pathway via BMP7. a shows the expression of proteins related to TGF-β signaling pathway. b–h are greyscale scans of proteins in A. h shows Desmin expression in C2C12 cells when SPARCL1 was activated and BMP7 was inhibited at 72 h. i is the corresponding immunoﬂuorescence blot of Desmin protein in A. In a, i, pSPgRNA-S-2 is the SPARCL1 activation group, while pSPgRNA is the SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β blank control for SPARCL1 activation. SiRNA-B is the BMP7 interference group. NC is a negative control for BMP7 siRNA interference. The scale bar in I is 100 μm, green ﬂuorescent signal is Desmin, and blue ﬂuorescent signal is the nucleus. **P values < 0.01 and *P values < 0.05 were considered as signiﬁcant

Journal: Cell death & disease

Article Title: SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway.

doi: 10.1038/s41419-019-2049-4

Figure Lengend Snippet: Fig. 7 SPARCL1 regulates C2C12 cell differentiation through BMP/TGF-β signaling pathway via BMP7. a shows the expression of proteins related to TGF-β signaling pathway. b–h are greyscale scans of proteins in A. h shows Desmin expression in C2C12 cells when SPARCL1 was activated and BMP7 was inhibited at 72 h. i is the corresponding immunoﬂuorescence blot of Desmin protein in A. In a, i, pSPgRNA-S-2 is the SPARCL1 activation group, while pSPgRNA is the SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β blank control for SPARCL1 activation. SiRNA-B is the BMP7 interference group. NC is a negative control for BMP7 siRNA interference. The scale bar in I is 100 μm, green ﬂuorescent signal is Desmin, and blue ﬂuorescent signal is the nucleus. **P values < 0.01 and *P values < 0.05 were considered as signiﬁcant

Techniques: Cell Differentiation, Expressing, Activation Assay, Control, Negative Control

Fig. 8 Scheme of SPARCL1 affects C2C12 cell differentiation via BMP7/TGF-β pathway. SPARCL1 interacts with BMP7, BMP7 acts on TGF-β pathway receptors, and activation pathway affects cell differentiation

Journal: Cell death & disease

Article Title: SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway.

doi: 10.1038/s41419-019-2049-4

Figure Lengend Snippet: Fig. 8 Scheme of SPARCL1 affects C2C12 cell differentiation via BMP7/TGF-β pathway. SPARCL1 interacts with BMP7, BMP7 acts on TGF-β pathway receptors, and activation pathway affects cell differentiation

Techniques: Cell Differentiation, Activation Assay

Secreted protein acidic and rich in cysteine like 1 (SPARCL1) interacts with integrin β1 (ITGB1). ( A ) Co-immunoprecipitation with an anti-SPARCL1 antibody followed by Western blotting with an anti-ITGB1 antibody. ( B ) Co-immunoprecipitation with an anti-ITGB1 antibody followed by Western blotting with an anti-SPARCL1 antibody. Input, IgG, and IP represent the positive control, negative control, and target experimental group, respectively.

Journal: Animals : an Open Access Journal from MDPI

Article Title: SPARCL1 Influences Bovine Skeletal Muscle-Derived Satellite Cell Migration and Differentiation through an ITGB1-Mediated Signaling Pathway

doi: 10.3390/ani10081361

Figure Lengend Snippet: Secreted protein acidic and rich in cysteine like 1 (SPARCL1) interacts with integrin β1 (ITGB1). ( A ) Co-immunoprecipitation with an anti-SPARCL1 antibody followed by Western blotting with an anti-ITGB1 antibody. ( B ) Co-immunoprecipitation with an anti-ITGB1 antibody followed by Western blotting with an anti-SPARCL1 antibody. Input, IgG, and IP represent the positive control, negative control, and target experimental group, respectively.

Article Snippet: Rabbit anti-SPARCL1 antibody (bs-6110R), rabbit anti-integrin beta 1 antibody (bs-0486R), rabbit anti-FAK antibody (bs-20735R), rabbit anti-paxillin antibody (bs-3539R), rabbit anti-phospho-FAK (Tyr397) antibody (bs-3159R), rabbit anti-phospho-paxillin (Tyr118) antibody (bs-3352R), rabbit anti-vinculin antibody (bs-6640R), rabbit anti-ARP2/3 subunit 1B antibody (bs-10563R), rabbit anti-CDC42 antibody (bs-3555R), rabbit anti-MyoD antibody (bs-2442R), rabbit anti-GAPDH antibody (bs-0755R) (all at dilution 1:500; Bioss Antibodies, China).

Techniques: Immunoprecipitation, Western Blot, Positive Control, Negative Control

SPARCL1 influences MDSC migration and differentiation. ( A , C ) Cell scratch assay images at 20 h after SPARCL1 activation or inhibition. ( B , D ) Quantification of the cell migration rate at 20 h after SPARCL1 activation or inhibition according to ( A , C ). ( E , G ) Desmin immunofluorescence staining in MDSCs after SPARCL1 activation or inhibition. ( F , H ) Quantification of the myotube rate based on ( E , G ). ( I , K ) Western blotting images of MyoD expression after SPARCL1 activation or inhibition. ( J , L ) Quantification of MyoD from Western blotting results presented in ( I , K ). Scale bar = 100 μm. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Animals : an Open Access Journal from MDPI

Article Title: SPARCL1 Influences Bovine Skeletal Muscle-Derived Satellite Cell Migration and Differentiation through an ITGB1-Mediated Signaling Pathway

doi: 10.3390/ani10081361

Figure Lengend Snippet: SPARCL1 influences MDSC migration and differentiation. ( A , C ) Cell scratch assay images at 20 h after SPARCL1 activation or inhibition. ( B , D ) Quantification of the cell migration rate at 20 h after SPARCL1 activation or inhibition according to ( A , C ). ( E , G ) Desmin immunofluorescence staining in MDSCs after SPARCL1 activation or inhibition. ( F , H ) Quantification of the myotube rate based on ( E , G ). ( I , K ) Western blotting images of MyoD expression after SPARCL1 activation or inhibition. ( J , L ) Quantification of MyoD from Western blotting results presented in ( I , K ). Scale bar = 100 μm. * p < 0.05, ** p < 0.01, *** p < 0.001.

Techniques: Migration, Wound Healing Assay, Activation Assay, Inhibition, Immunofluorescence, Staining, Western Blot, Expressing

SPARCL1 affects an ITGB1-mediated signaling pathway. ( A , I ) Western blotting results for ITGB1, p-FAK, FAK, p-paxillin, paxillin, vinculin, Cdc42, and Arp2/3 after SPARCL1 activation or inhibition, respectively. ( B – H ) Quantification of the Western blotting results presented in ( A ). ( J – P ) Quantification of the Western blotting results presented in ( I ). ** p < 0.01.

Journal: Animals : an Open Access Journal from MDPI

Article Title: SPARCL1 Influences Bovine Skeletal Muscle-Derived Satellite Cell Migration and Differentiation through an ITGB1-Mediated Signaling Pathway

doi: 10.3390/ani10081361

Figure Lengend Snippet: SPARCL1 affects an ITGB1-mediated signaling pathway. ( A , I ) Western blotting results for ITGB1, p-FAK, FAK, p-paxillin, paxillin, vinculin, Cdc42, and Arp2/3 after SPARCL1 activation or inhibition, respectively. ( B – H ) Quantification of the Western blotting results presented in ( A ). ( J – P ) Quantification of the Western blotting results presented in ( I ). ** p < 0.01.

Techniques: Western Blot, Activation Assay, Inhibition

SPARCL1 influences cell migration and differentiation through ITGB1. ( A ) Cell scratch assay images after simultaneous SPARCL1 activation and ITGB1 inhibition. ( B ) Quantification of the cell migration rate based on ( A ). ( C ) Desmin immunofluorescence staining of MDSCs after SPARCL1 activation and ITGB1 inhibition. ( D ) Quantification of the myotube rate represented in ( C ). ( E ) Western blotting results of MyoD expression after SPARCL1 activation and ITGB1 inhibition. ( F ) Quantification of the MyoD Western blotting results presented in ( E ). pSPgRNA is the blank control for the SPARCL1 activation group, NC is the negative control for the ITGB1 siRNA interference group, VPR-S represents the SPARCL1 activation group, and siRNA-I represents the ITGB1 siRNA interference group. * p < 0.05, ** p < 0.01, *** p < 0.001; scale bar = 100 μm.

Journal: Animals : an Open Access Journal from MDPI

Article Title: SPARCL1 Influences Bovine Skeletal Muscle-Derived Satellite Cell Migration and Differentiation through an ITGB1-Mediated Signaling Pathway

doi: 10.3390/ani10081361

Figure Lengend Snippet: SPARCL1 influences cell migration and differentiation through ITGB1. ( A ) Cell scratch assay images after simultaneous SPARCL1 activation and ITGB1 inhibition. ( B ) Quantification of the cell migration rate based on ( A ). ( C ) Desmin immunofluorescence staining of MDSCs after SPARCL1 activation and ITGB1 inhibition. ( D ) Quantification of the myotube rate represented in ( C ). ( E ) Western blotting results of MyoD expression after SPARCL1 activation and ITGB1 inhibition. ( F ) Quantification of the MyoD Western blotting results presented in ( E ). pSPgRNA is the blank control for the SPARCL1 activation group, NC is the negative control for the ITGB1 siRNA interference group, VPR-S represents the SPARCL1 activation group, and siRNA-I represents the ITGB1 siRNA interference group. * p < 0.05, ** p < 0.01, *** p < 0.001; scale bar = 100 μm.

Techniques: Migration, Wound Healing Assay, Activation Assay, Inhibition, Immunofluorescence, Staining, Western Blot, Expressing, Negative Control

SPARCL1 regulates an ITGB1-mediated signaling pathway through ITGB1. ( A ) Western blotting results for ITGB1, p-FAK, FAK, p-paxillin, paxillin, vinculin, Cdc42, and Arp2/3 expression after SPARCL1 activation and ITGB1 inhibition. ( B – H ) Quantification of the Western blotting results presented in ( A ). pSPgRNA is the blank control for the SPARCL1 activation group, NC is the negative control for the ITGB1 siRNA interference group, VPR-S represents the SPARCL1 activation group, and siRNA-I represents the ITGB1 siRNA interference group. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Animals : an Open Access Journal from MDPI

Article Title: SPARCL1 Influences Bovine Skeletal Muscle-Derived Satellite Cell Migration and Differentiation through an ITGB1-Mediated Signaling Pathway

doi: 10.3390/ani10081361

Figure Lengend Snippet: SPARCL1 regulates an ITGB1-mediated signaling pathway through ITGB1. ( A ) Western blotting results for ITGB1, p-FAK, FAK, p-paxillin, paxillin, vinculin, Cdc42, and Arp2/3 expression after SPARCL1 activation and ITGB1 inhibition. ( B – H ) Quantification of the Western blotting results presented in ( A ). pSPgRNA is the blank control for the SPARCL1 activation group, NC is the negative control for the ITGB1 siRNA interference group, VPR-S represents the SPARCL1 activation group, and siRNA-I represents the ITGB1 siRNA interference group. * p < 0.05, ** p < 0.01, *** p < 0.001.

Techniques: Western Blot, Expressing, Activation Assay, Inhibition, Negative Control

Proposed model for SPARCL1 regulation of bovine MDSC migration and differentiation through ITGB1. As an extracellular matrix protein, SPARCL1 interacts with the transmembrane receptor ITGB1 and regulates changes in the expression of adhesion-plaque-associated proteins, such as ITGB1, p-FAK, FAK, p-paxillin, paxillin, and vinculin to affect the formation of focal adhesions. The Cdc42 and Arp2/3 complex was also affected by SPARCL1 through ITGB1. Further, bovine MDSC migration was stimulated by SPARCL1, and cells gathered and apparently expressed MyoD to begin an early-stage differentiation.

Journal: Animals : an Open Access Journal from MDPI

Article Title: SPARCL1 Influences Bovine Skeletal Muscle-Derived Satellite Cell Migration and Differentiation through an ITGB1-Mediated Signaling Pathway

doi: 10.3390/ani10081361

Figure Lengend Snippet: Proposed model for SPARCL1 regulation of bovine MDSC migration and differentiation through ITGB1. As an extracellular matrix protein, SPARCL1 interacts with the transmembrane receptor ITGB1 and regulates changes in the expression of adhesion-plaque-associated proteins, such as ITGB1, p-FAK, FAK, p-paxillin, paxillin, and vinculin to affect the formation of focal adhesions. The Cdc42 and Arp2/3 complex was also affected by SPARCL1 through ITGB1. Further, bovine MDSC migration was stimulated by SPARCL1, and cells gathered and apparently expressed MyoD to begin an early-stage differentiation.

Techniques: Migration, Expressing

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