bs-6110R Search Results


sparcl1 catalog  (Bioss)
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Bioss sparcl1 catalog
Fig. 1 <t>SPARCL1</t> influences C2C12 cell differentiation. a, e shows the expression of SPARCL1 protein activated or inhibited in C2C12 cells when the cells were induced to differentiate at 72 h. pSPgRNA-S-2 is the SPARCL1 activation group and pSPgRNA is the blank control for SPARCL1 activation. NC was the negative control for SPARCL1 siRNA interference. b–d are grayscale scans of the proteins shown in a. f–h are grayscale scans of the proteins shown in e. i, k show Desmin expression in C2C12 cells when SPARCL1 was activated or inhibited at 72 h. j, l shows the quantification of myotubes according to the Desmin staining of I and K. The scale bar in I and K is 100 μm; the green fluorescent signal is Desmin, while the blue fluorescent signal is the nucleus. **P < 0.01 were considered as significant
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Fig. 1 SPARCL1 influences C2C12 cell differentiation. a, e shows the expression of SPARCL1 protein activated or inhibited in C2C12 cells when the cells were induced to differentiate at 72 h. pSPgRNA-S-2 is the SPARCL1 activation group and pSPgRNA is the blank control for SPARCL1 activation. NC was the negative control for SPARCL1 siRNA interference. b–d are grayscale scans of the proteins shown in a. f–h are grayscale scans of the proteins shown in e. i, k show Desmin expression in C2C12 cells when SPARCL1 was activated or inhibited at 72 h. j, l shows the quantification of myotubes according to the Desmin staining of I and K. The scale bar in I and K is 100 μm; the green fluorescent signal is Desmin, while the blue fluorescent signal is the nucleus. **P < 0.01 were considered as significant

Journal: Cell death & disease

Article Title: SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway.

doi: 10.1038/s41419-019-2049-4

Figure Lengend Snippet: Fig. 1 SPARCL1 influences C2C12 cell differentiation. a, e shows the expression of SPARCL1 protein activated or inhibited in C2C12 cells when the cells were induced to differentiate at 72 h. pSPgRNA-S-2 is the SPARCL1 activation group and pSPgRNA is the blank control for SPARCL1 activation. NC was the negative control for SPARCL1 siRNA interference. b–d are grayscale scans of the proteins shown in a. f–h are grayscale scans of the proteins shown in e. i, k show Desmin expression in C2C12 cells when SPARCL1 was activated or inhibited at 72 h. j, l shows the quantification of myotubes according to the Desmin staining of I and K. The scale bar in I and K is 100 μm; the green fluorescent signal is Desmin, while the blue fluorescent signal is the nucleus. **P < 0.01 were considered as significant

Article Snippet: The membrane was blocked with 5% skimmed milk/PBST for 60min at 37 °C, and then the membranes were incubated with primary antibodies (SPARCL1 (catalog number: bs-6110R), BMP7 (catalog number: bs-2242R), MYOG (catalog number: bs-3550R), GAPDH (catalog number: bs-0755R), SMAD4 (catalog number: bs23966R), Desmin (catalog number: bs-20702R), all from Bioss and P-SMAD4 (catalog number: AF8316) from Affinty USA) overnight at 4 °C.

Techniques: Cell Differentiation, Expressing, Activation Assay, Control, Negative Control, Staining

Fig. 2 SPARCL1 expressed in mice muscle injury model. a shows HE staining of TA muscles injury. b shows the Protein expression changes of SPARCL1 in muscle damage repair, MyoD, MHC, Pax7+ are marker molecules involved in cell differentiation, c–f are grayscale scans of the relevant proteins in b. g shows the SPARCL1 expressed in mouse muscle injury model. The green fluorescent signal is SPARCL1, red fluorescent signal is laminin, and blue fluorescent signal is cell nucleus. H shows statistical data for positive cell number based on SPARCL1 expression in 2G. Five fields of view per experimental group were used to analyse SPARCL1 expression in TA muscle cells. D0, D1, D3, D5, and D7 represent TA muscle damage on days 0, 1, 3, 5, and 7, respectively. The scale bar in A and B is 100 μm. **P values < 0.01 and *P values < 0.05 were considered as significant

Journal: Cell death & disease

Article Title: SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway.

doi: 10.1038/s41419-019-2049-4

Figure Lengend Snippet: Fig. 2 SPARCL1 expressed in mice muscle injury model. a shows HE staining of TA muscles injury. b shows the Protein expression changes of SPARCL1 in muscle damage repair, MyoD, MHC, Pax7+ are marker molecules involved in cell differentiation, c–f are grayscale scans of the relevant proteins in b. g shows the SPARCL1 expressed in mouse muscle injury model. The green fluorescent signal is SPARCL1, red fluorescent signal is laminin, and blue fluorescent signal is cell nucleus. H shows statistical data for positive cell number based on SPARCL1 expression in 2G. Five fields of view per experimental group were used to analyse SPARCL1 expression in TA muscle cells. D0, D1, D3, D5, and D7 represent TA muscle damage on days 0, 1, 3, 5, and 7, respectively. The scale bar in A and B is 100 μm. **P values < 0.01 and *P values < 0.05 were considered as significant

Article Snippet: The membrane was blocked with 5% skimmed milk/PBST for 60min at 37 °C, and then the membranes were incubated with primary antibodies (SPARCL1 (catalog number: bs-6110R), BMP7 (catalog number: bs-2242R), MYOG (catalog number: bs-3550R), GAPDH (catalog number: bs-0755R), SMAD4 (catalog number: bs23966R), Desmin (catalog number: bs-20702R), all from Bioss and P-SMAD4 (catalog number: AF8316) from Affinty USA) overnight at 4 °C.

Techniques: Staining, Muscles, Expressing, Marker, Cell Differentiation

Fig. 3 SPARCL1 interacted with BMP7. a shows the Co-IP results of SPARCL1 SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β protein interacting with BMP7 protein. b shows the Co-IP results of BMP7 protein interacting with SPARCL1 protein. In a, b, Input indicates the positive control group, IgG indicates the negative control group, IP indicates the target experimental group, and IB indicates the verification of SPARCL1 and BMP7 in a and b, respectively

Journal: Cell death & disease

Article Title: SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway.

doi: 10.1038/s41419-019-2049-4

Figure Lengend Snippet: Fig. 3 SPARCL1 interacted with BMP7. a shows the Co-IP results of SPARCL1 SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β protein interacting with BMP7 protein. b shows the Co-IP results of BMP7 protein interacting with SPARCL1 protein. In a, b, Input indicates the positive control group, IgG indicates the negative control group, IP indicates the target experimental group, and IB indicates the verification of SPARCL1 and BMP7 in a and b, respectively

Article Snippet: The membrane was blocked with 5% skimmed milk/PBST for 60min at 37 °C, and then the membranes were incubated with primary antibodies (SPARCL1 (catalog number: bs-6110R), BMP7 (catalog number: bs-2242R), MYOG (catalog number: bs-3550R), GAPDH (catalog number: bs-0755R), SMAD4 (catalog number: bs23966R), Desmin (catalog number: bs-20702R), all from Bioss and P-SMAD4 (catalog number: AF8316) from Affinty USA) overnight at 4 °C.

Techniques: Co-Immunoprecipitation Assay, Cell Differentiation, Positive Control, Negative Control

Fig. 5 BMP7 influences C2C12 cells differentiation. a, e show the expression of BMP7 protein activated or inhibited in C2C12 cells when the cells were induced to differentiate at 72 h. pSPgRNA-B-3 was the BMP7 activation group, pSPgRNA was the blank control for BMP7 activation. NC was a negative control for BMP7 siRNA interference. b–d shows greyscale scans of the proteins in A. f–h shows greyscale scans of the proteins in e. i, k shows Desmin expression in C2C12 cells when SPARCL1 was activated or inhibited at 72 h. j, l shows quantification of myotubes according to Desmin staining in i, k, respectively. The SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β scale bar in i, k is 100 μm, and the green fluorescent signal is Desmin, while the blue fluorescent signal is the nucleus. **P values < 0.01 and *P values < 0.05 were considered as significant

Journal: Cell death & disease

Article Title: SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway.

doi: 10.1038/s41419-019-2049-4

Figure Lengend Snippet: Fig. 5 BMP7 influences C2C12 cells differentiation. a, e show the expression of BMP7 protein activated or inhibited in C2C12 cells when the cells were induced to differentiate at 72 h. pSPgRNA-B-3 was the BMP7 activation group, pSPgRNA was the blank control for BMP7 activation. NC was a negative control for BMP7 siRNA interference. b–d shows greyscale scans of the proteins in A. f–h shows greyscale scans of the proteins in e. i, k shows Desmin expression in C2C12 cells when SPARCL1 was activated or inhibited at 72 h. j, l shows quantification of myotubes according to Desmin staining in i, k, respectively. The SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β scale bar in i, k is 100 μm, and the green fluorescent signal is Desmin, while the blue fluorescent signal is the nucleus. **P values < 0.01 and *P values < 0.05 were considered as significant

Article Snippet: The membrane was blocked with 5% skimmed milk/PBST for 60min at 37 °C, and then the membranes were incubated with primary antibodies (SPARCL1 (catalog number: bs-6110R), BMP7 (catalog number: bs-2242R), MYOG (catalog number: bs-3550R), GAPDH (catalog number: bs-0755R), SMAD4 (catalog number: bs23966R), Desmin (catalog number: bs-20702R), all from Bioss and P-SMAD4 (catalog number: AF8316) from Affinty USA) overnight at 4 °C.

Techniques: Expressing, Activation Assay, Control, Negative Control, Staining, Cell Differentiation

Fig. 6 SPARCL1 regulates BMP7 expression and BMP/TGF-β cell signaling pathway. a, d shows the protein expression of BMP7 regulated by SPARCL1 activation and inhibition, respectively. C2C12 cells were induced to differentiate at 72 h. pSPgRNA-S-2 is the SPARCL1 activation group, while pSPgRNA is the blank control for SPARCL1 activation. NC is the negative control for SPARCL1 siRNA interference. b, c are greyscale scans of SPARCL1 and BMP7 proteins in A. e, f are grayscale scans of SPARCL1 and BMP7 proteins in D. i and p shows changes in the expression of BMP/ TGF-β-associated proteins when SPARCL1 was activated and inhibited, respectively, and C2C12 cells were induced to differentiate at 72 h. pSPgRNA-S-2 is the SPARCL1 activation group, while pSPgRNA is the blank control for SPARCL1 activation. NC is the negative control for SPARCL1 siRNA interference. j–o are greyscale scans of proteins in I. q–v are greyscale scans of proteins in P. **P values < 0.01 were considered as significant

Journal: Cell death & disease

Article Title: SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway.

doi: 10.1038/s41419-019-2049-4

Figure Lengend Snippet: Fig. 6 SPARCL1 regulates BMP7 expression and BMP/TGF-β cell signaling pathway. a, d shows the protein expression of BMP7 regulated by SPARCL1 activation and inhibition, respectively. C2C12 cells were induced to differentiate at 72 h. pSPgRNA-S-2 is the SPARCL1 activation group, while pSPgRNA is the blank control for SPARCL1 activation. NC is the negative control for SPARCL1 siRNA interference. b, c are greyscale scans of SPARCL1 and BMP7 proteins in A. e, f are grayscale scans of SPARCL1 and BMP7 proteins in D. i and p shows changes in the expression of BMP/ TGF-β-associated proteins when SPARCL1 was activated and inhibited, respectively, and C2C12 cells were induced to differentiate at 72 h. pSPgRNA-S-2 is the SPARCL1 activation group, while pSPgRNA is the blank control for SPARCL1 activation. NC is the negative control for SPARCL1 siRNA interference. j–o are greyscale scans of proteins in I. q–v are greyscale scans of proteins in P. **P values < 0.01 were considered as significant

Article Snippet: The membrane was blocked with 5% skimmed milk/PBST for 60min at 37 °C, and then the membranes were incubated with primary antibodies (SPARCL1 (catalog number: bs-6110R), BMP7 (catalog number: bs-2242R), MYOG (catalog number: bs-3550R), GAPDH (catalog number: bs-0755R), SMAD4 (catalog number: bs23966R), Desmin (catalog number: bs-20702R), all from Bioss and P-SMAD4 (catalog number: AF8316) from Affinty USA) overnight at 4 °C.

Techniques: Expressing, Activation Assay, Inhibition, Control, Negative Control

Fig. 7 SPARCL1 regulates C2C12 cell differentiation through BMP/TGF-β signaling pathway via BMP7. a shows the expression of proteins related to TGF-β signaling pathway. b–h are greyscale scans of proteins in A. h shows Desmin expression in C2C12 cells when SPARCL1 was activated and BMP7 was inhibited at 72 h. i is the corresponding immunofluorescence blot of Desmin protein in A. In a, i, pSPgRNA-S-2 is the SPARCL1 activation group, while pSPgRNA is the SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β blank control for SPARCL1 activation. SiRNA-B is the BMP7 interference group. NC is a negative control for BMP7 siRNA interference. The scale bar in I is 100 μm, green fluorescent signal is Desmin, and blue fluorescent signal is the nucleus. **P values < 0.01 and *P values < 0.05 were considered as significant

Journal: Cell death & disease

Article Title: SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway.

doi: 10.1038/s41419-019-2049-4

Figure Lengend Snippet: Fig. 7 SPARCL1 regulates C2C12 cell differentiation through BMP/TGF-β signaling pathway via BMP7. a shows the expression of proteins related to TGF-β signaling pathway. b–h are greyscale scans of proteins in A. h shows Desmin expression in C2C12 cells when SPARCL1 was activated and BMP7 was inhibited at 72 h. i is the corresponding immunofluorescence blot of Desmin protein in A. In a, i, pSPgRNA-S-2 is the SPARCL1 activation group, while pSPgRNA is the SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β blank control for SPARCL1 activation. SiRNA-B is the BMP7 interference group. NC is a negative control for BMP7 siRNA interference. The scale bar in I is 100 μm, green fluorescent signal is Desmin, and blue fluorescent signal is the nucleus. **P values < 0.01 and *P values < 0.05 were considered as significant

Article Snippet: The membrane was blocked with 5% skimmed milk/PBST for 60min at 37 °C, and then the membranes were incubated with primary antibodies (SPARCL1 (catalog number: bs-6110R), BMP7 (catalog number: bs-2242R), MYOG (catalog number: bs-3550R), GAPDH (catalog number: bs-0755R), SMAD4 (catalog number: bs23966R), Desmin (catalog number: bs-20702R), all from Bioss and P-SMAD4 (catalog number: AF8316) from Affinty USA) overnight at 4 °C.

Techniques: Cell Differentiation, Expressing, Activation Assay, Control, Negative Control

Fig. 8 Scheme of SPARCL1 affects C2C12 cell differentiation via BMP7/TGF-β pathway. SPARCL1 interacts with BMP7, BMP7 acts on TGF-β pathway receptors, and activation pathway affects cell differentiation

Journal: Cell death & disease

Article Title: SPARCL1 promotes C2C12 cell differentiation via BMP7-mediated BMP/TGF-β cell signaling pathway.

doi: 10.1038/s41419-019-2049-4

Figure Lengend Snippet: Fig. 8 Scheme of SPARCL1 affects C2C12 cell differentiation via BMP7/TGF-β pathway. SPARCL1 interacts with BMP7, BMP7 acts on TGF-β pathway receptors, and activation pathway affects cell differentiation

Article Snippet: The membrane was blocked with 5% skimmed milk/PBST for 60min at 37 °C, and then the membranes were incubated with primary antibodies (SPARCL1 (catalog number: bs-6110R), BMP7 (catalog number: bs-2242R), MYOG (catalog number: bs-3550R), GAPDH (catalog number: bs-0755R), SMAD4 (catalog number: bs23966R), Desmin (catalog number: bs-20702R), all from Bioss and P-SMAD4 (catalog number: AF8316) from Affinty USA) overnight at 4 °C.

Techniques: Cell Differentiation, Activation Assay