mst1  (Bioss)

Journal: International Journal of Oncology

Article Title: ARID1A restrains EMT and stemness of ovarian cancer cells through the Hippo pathway

doi: 10.3892/ijo.2024.5664

Figure Lengend Snippet: ARID1A controls Hippo signaling activity in ovarian cancer cells. (A) SK-OV-3 cells were transfected with ARID1A-overexpressing (ARID1A + ) or control vector (ARID1A - ) and protein levels of pMST1, MST1, pLATS1 and LATS1 were measured by WB after 12 h. GAPDH was used as loading control. (B) SK-OV-3 cells were transfected with ARID1A-shRNA (shARID1A + ) or control shRNA (shARID1A - ) and protein levels of pMST1, MST1, pLATS1 and LATS1 were measured by WB after 24 h. GAPDH was used as loading control. (C) SK-OV-3 cells were transfected with ARID1A-overexpressing (ARID1A + ) or control vector (ARID1A-) and protein levels of p-TAZ, TAZ, p-YAP and YAP were measured by WB after 12 h. GAPDH was used as loading control. (D) SK-OV-3 cells were transfected with ARID1A-shRNA (shARID1A + ) or control shRNA (shARID1A - ) and protein levels of pTAZ, TAZ, pYAP and YAP were measured by WB after 24 h. GAPDH was used as loading control. (E) SK-OV-3 cells were transfected with ARID1A-overexpressing (ARID1A + ) or control vector (ARID1A - ) and protein levels of CTGF and CYR61 were measured by WB after 12 h. GAPDH was used as loading control. (F) SK-OV-3 cells were transfected with ARID1A-shRNA (shARID1A + ) or control shRNA (shARID1A-) and protein levels of CTGF and CYR61 were measured by WB after 24 h. GAPDH was used as loading control. (G) SK-OV-3 cells were transfected with ARID1A-overexpressing (ARID1A) or control vector (Control) and mRNA levels of CTGF and CYR61 were measured by RT-qPCR. (H) SK-OV-3 cells were transfected with ARID1A-shRNA or control shRNA (Scramble) and mRNA levels of CTGF and CYR61 were measured by RT-qPCR. (I) A2780 cells were transfected with ARID1A-overexpressing (ARID1A) or control vector (Control) and mRNA levels of CTGF and CYR61 were measured by RT-qPCR. (J) A2780 cells were transfected with ARID1A-shRNA or control shRNA (Scramble) and mRNA levels of CTGF and CYR61 were measured by RT-qPCR. * P<0.05 and ** P<0.01 (n=3). ARID1A, AT-rich binding domain 1A; p-, phosphorylated; shRNA, short hairpin RNA; TAZ, transcriptional co-activator with PDZ-binding motif; RT-qPCR, reverse transcription-quantitative PCR; WB, western blotting.

Article Snippet: Antibodies for ARID1A (mouse; cat. no. sc-32761), YAP (cat. no. sc-101199), CTGF (cat. no. sc-373936), CYR61 (cat. no. sc-374129), Nanog (cat. no. sc-293121), Sox2 (cat. no. sc-365823), Oct3/4 (cat. no. sc-5279), GAPDH (cat. no. sc-32233) and normal mouse IgG (cat. no. sc-2025) were purchased from Santa Cruz Biotechnology, Inc. Antibodies for p-YAP antibody (cat. no. AF5965), α-Tubulin (cat. no. AF0001), N-cadherin (cat. no. AF5237) and E-cadherin (cat. no. AF0138) were obtained from Beyotime Institute of Biotechnology. p-MST1 (cat. no. bs-3294R), MST1 (cat. no. bs-3504R), p-LATS1 (cat. no. bs-3245R), LATS1 (cat. no. bs-2904R), TAZ (cat. no. bs-12367R) and Vimentin (cat. no. bs-23063R) antibodies were purchased from BIOSS.

Techniques: Activity Assay, Transfection, Control, Plasmid Preparation, shRNA, Quantitative RT-PCR, Binding Assay, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot

Journal: Pakistan Journal of Zoology

Article Title: Tetrandrine Induces Apoptosis in HepG2 by Modulating Hippo Signalling Pathway

doi: 10.17582/journal.pjz/20230410080440

Figure Lengend Snippet: Fig. 4. Protein expression profile after 48 h TET treatment (3.75, 7.5, or 15 μmol/L) HepG2 cells. Western blots represent (A) MST1, (B) LATS1, (C) P-LATS1, and (D) P-YAP1. (**P<0.01, vs control).

Article Snippet: Protein transfer was done onto nitrocellulose membrane and blocking was carried out with 5% skim milk for 1 h after which incubation was done with primary antibodies (rabbit anti-human binding immunoglobulin protein; MST1, bs-3504R; LATS1, bs2904R; P-LATS1, bs-7913R; YAP1, bs-52418R; P-YAP1, bs-3475R; TAZ, bs-12367R; BIOSS) at 4 °C overnight.

Techniques: Expressing, Western Blot, Control

Journal: Pakistan Journal of Zoology

Article Title: Tetrandrine Induces Apoptosis in HepG2 by Modulating Hippo Signalling Pathway

doi: 10.17582/journal.pjz/20230410080440

Figure Lengend Snippet: Fig. 5. Expression profile proteins participating in Hippo signalling pathway in HepG2 cells upon 48 h TET treatment (3.75, 7.5, or 15 μmol/L). Western blots represent (A) YAP1 and (B) TAZ. (**P<0.01, *P<0.05).

Article Snippet: Protein transfer was done onto nitrocellulose membrane and blocking was carried out with 5% skim milk for 1 h after which incubation was done with primary antibodies (rabbit anti-human binding immunoglobulin protein; MST1, bs-3504R; LATS1, bs2904R; P-LATS1, bs-7913R; YAP1, bs-52418R; P-YAP1, bs-3475R; TAZ, bs-12367R; BIOSS) at 4 °C overnight.

Techniques: Expressing, Western Blot