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polyclonal antibody  (Bioss)


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    Bioss polyclonal antibody
    Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibody/product/Bioss
    Average 94 stars, based on 3 article reviews
    polyclonal antibody - by Bioz Stars, 2026-02
    94/100 stars

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    Image Search Results


    (A) Diagram showing the experimental design. Rabbits central and peripheral CECs were taken for RNA sequencing separately on day 7 after injury. (B) Volcano plot of the significant DEGs between the central and peripheral CECs. (C) The KEGG enrichment of DEGs in (B). (D) The core proteins of the top 100 PPIs. (E) The top 10 of KEGG enrichment for genes related to the top 100 of PPIs in (D). (F) The heatmap of downstream genes of the Hippo pathway. (G, H) Western blot analysis of p-YAP, YAP, p-MST1/2, MST1/2, p-LATS1/2, LATS1/2 and β-actin, the protein levels were quantified by densitometry (n=3). (I) Representative immunofluorescence images of YAP and the ratio of nuclear YAP to cytoplasm YAP, five cells were randomly selected for measurement and averaged in each sample (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, error bars, mean ± SEM. Scale bars represent 10 µm in (I). Nuc, nucleus; Cyt, cytoplasm.

    Journal: bioRxiv

    Article Title: The Hippo Signaling Pathway Regulates Corneal Endothelial Regeneration

    doi: 10.64898/2025.12.30.697046

    Figure Lengend Snippet: (A) Diagram showing the experimental design. Rabbits central and peripheral CECs were taken for RNA sequencing separately on day 7 after injury. (B) Volcano plot of the significant DEGs between the central and peripheral CECs. (C) The KEGG enrichment of DEGs in (B). (D) The core proteins of the top 100 PPIs. (E) The top 10 of KEGG enrichment for genes related to the top 100 of PPIs in (D). (F) The heatmap of downstream genes of the Hippo pathway. (G, H) Western blot analysis of p-YAP, YAP, p-MST1/2, MST1/2, p-LATS1/2, LATS1/2 and β-actin, the protein levels were quantified by densitometry (n=3). (I) Representative immunofluorescence images of YAP and the ratio of nuclear YAP to cytoplasm YAP, five cells were randomly selected for measurement and averaged in each sample (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, error bars, mean ± SEM. Scale bars represent 10 µm in (I). Nuc, nucleus; Cyt, cytoplasm.

    Article Snippet: The equal amount of protein was resolved by SDS-PAGE and immunoblotted with primary antibodies for YAP1 (Abclonal, A19134), phospho-YAP1-S127 (Abcam, ab186735), LATS1/2 (Proteintech, 17049-1-AP), phospho-LATS1/2 (Thr1079) (Proteintech, 28998-1-AP), MST1/2 (Proteintech, 22245-1-AP), phospho-MST1 (Thr183)/MST2 (Thr180) (Proteintech, 80093-1-RR), MOB1 (Proteintech, 11669-1-AP), phospho-MOB1 (Thr12) (Proteintech, 29027-1-AP) and β-actin (Abcam, 8226).

    Techniques: RNA Sequencing, Western Blot, Immunofluorescence