sglt1  (Bioss)

Journal: Frontiers in Nutrition

Article Title: Maternal docosahexaenoic acid supplementation during lactation improves exercise performance, enhances intestinal glucose absorption and modulates gut microbiota in weaning offspring mice

doi: 10.3389/fnut.2024.1423576

Figure Lengend Snippet: Effect of maternal DHA supplementation during lactation on the jejunal glucose transport-associated gene expression in weaning mice. Representative Western blots of (A) GLUT2 and (B) SGLT1, and (D,F) their relative band intensities in jejunal tissue of mice from different groups. n = 4 per group. Relative mRNA levels of (C) GLUT2 and (E) SGLT1 in jejunal tissue of mice from different groups. n = 7–8 per group. Data were expressed as the mean ± SEM. Significance was set at p < 0.05 ( * p < 0.05, ** p < 0.01). L-DHA: 150 mg/(kg body weight day) DHA; H-DHA: 450 mg/(kg body weight · day) DHA. DHA, docosahexaenoic acid; GLUT2, glucose transporter 2; SGLT1, sodium glucose co-transporter 1.

Article Snippet: After transfer, the PVDF membrane was blocked for 1 h in 5% fat-free milk and then was, respectively, incubated overnight at 4°C with dilutions of primary antibodies: GLUT2 (A12307, 1:2000, Abclonal, China), SGLT1 (bs-1128R, 1:2000, Bioss, China), S6 ribosomal protein (abs131865, 1:2000, Absin, China), p-Ser240/244-S6 ribosomal protein (ab215214, 1:2000, Abcam, UK), p70S6 kinase (14485-1-AP, 1,1,000, Proteintech, China), p-Thr389-p70S6 kinase (9,234, 1,1,000, Cell Signaling Technology, United States), mammalian target of rapamycin (mTOR, T55306, 1:2000, Abmart, China), p-S2448-mTOR (5,536, 1,1,000, Cell Signaling Technology), β-actin (T40104, 1:4000, Abmart).

Techniques: Expressing, Western Blot

Journal: PeerJ

Article Title: Adrenal SGLT1 or SGLT2 as predictors of atherosclerosis under chronic stress based on a computer algorithm

doi: 10.7717/peerj.15647

Figure Lengend Snippet: (A–B) Rose-colored SGLT1 fluorescence and pink-colored SGLT2 fluorescence could be seen in the adrenal cross sections of the four groups. (C) Semi-quantitative analysis of SGLT1 and SGLT2 expression in the adrenal glands of four groups showed that SGLT1 and SGLT2 expression levels were higher in mice after stress than in the corresponding non-stressed mice. (D) Expression of SGLT1 and SGLT2 was determined by Western blot analysis. (E) mRNA expression of SGLT1 and SGLT2 was detected by qPCR.

Article Snippet: Transverse sections of adrenal glands were incubated with SGLT1 Polyclonal Antibody (1:200, bs-1128R; BIOSS, Beijing, China) and SGLT2 Polyclonal Antibody (1:200, 24654-1-AP; Proteintech, Rosemont, IL, USA).

Techniques: Fluorescence, Expressing, Western Blot

Journal: PeerJ

Article Title: Adrenal SGLT1 or SGLT2 as predictors of atherosclerosis under chronic stress based on a computer algorithm

doi: 10.7717/peerj.15647

Figure Lengend Snippet: CYP11B2 labeled in red showed the adrenal cortex region, while tyrosine hydroxylase (TH) labeled in green showed the adrenal medulla. The nucleus was stained blue by DAPI. SGLT1 was detected by a rose and SGLT2 by a pink. The distribution of SGLT1 and SGLT2 in the adrenal glands of mice in the CON, CON+CS, HF+Apoe -/- , and HF+Apoe -/- +CS groups was observed to be altered by chronic stress.

Article Snippet: Transverse sections of adrenal glands were incubated with SGLT1 Polyclonal Antibody (1:200, bs-1128R; BIOSS, Beijing, China) and SGLT2 Polyclonal Antibody (1:200, 24654-1-AP; Proteintech, Rosemont, IL, USA).

Techniques: Labeling, Staining

Journal: PeerJ

Article Title: Adrenal SGLT1 or SGLT2 as predictors of atherosclerosis under chronic stress based on a computer algorithm

doi: 10.7717/peerj.15647

Figure Lengend Snippet: (A) The distribution of SGLT1 was observed under low and high magnification, and the differences in SGLT1 expression level in the adrenal gland of mice in the four groups were analyzed by semi-quantitative methods. (B) The distribution of SGLT2 was observed under high and low magnification in the adrenal gland, and the differences in expression between the four groups were analyzed. (C) Edema, glycogen content, and reticulocyte fiber breakage were positively correlated with the expression levels of SGLT1 and SGLT2 in the adrenal gland, respectively.* P < 0.05, ** P < 0.01, *** P < 0.001; ns =no significance.

Article Snippet: Transverse sections of adrenal glands were incubated with SGLT1 Polyclonal Antibody (1:200, bs-1128R; BIOSS, Beijing, China) and SGLT2 Polyclonal Antibody (1:200, 24654-1-AP; Proteintech, Rosemont, IL, USA).

Techniques: Expressing

Journal: PeerJ

Article Title: Adrenal SGLT1 or SGLT2 as predictors of atherosclerosis under chronic stress based on a computer algorithm

doi: 10.7717/peerj.15647

Figure Lengend Snippet: (A) Best training score of 0.0096775 with an epoch of 3000 after BP-neural network training for predicting intima-media thickness of abdominal aorta from SGLT1 and SGLT2 expression levels. (B) Relativity of 0.9841 with good correlation between input and output quantities. (C–D) Validation of the predicted data and the original values, with only small differences. (E) Best training score of 0.012396 with an epoch of 3000 after training the BP-neural network for predicting the internal diameter of abdominal aorta from SGLT1 and SGLT2 expression levels. (F) Relativity of 0.9805, with a good correlation between input and output volumes. (G–H) Validation of predicted data and original values.

Article Snippet: Transverse sections of adrenal glands were incubated with SGLT1 Polyclonal Antibody (1:200, bs-1128R; BIOSS, Beijing, China) and SGLT2 Polyclonal Antibody (1:200, 24654-1-AP; Proteintech, Rosemont, IL, USA).

Techniques: Expressing

Journal: PeerJ

Article Title: Adrenal SGLT1 or SGLT2 as predictors of atherosclerosis under chronic stress based on a computer algorithm

doi: 10.7717/peerj.15647

Figure Lengend Snippet: (A) The ROC curves showed that the expression level of SGLT1 in the adrenal gland sensitively and specifically predicted adrenal edema, broken reticular fibers, and glycogen content. (B) The ROC curves showed that the expression level of SGLT2 in the adrenal gland sensitively and specifically predicted adrenal edema, broken reticular fibers, and glycogen content.

Article Snippet: Transverse sections of adrenal glands were incubated with SGLT1 Polyclonal Antibody (1:200, bs-1128R; BIOSS, Beijing, China) and SGLT2 Polyclonal Antibody (1:200, 24654-1-AP; Proteintech, Rosemont, IL, USA).

Techniques: Expressing

Journal: PeerJ

Article Title: Adrenal SGLT1 or SGLT2 as predictors of atherosclerosis under chronic stress based on a computer algorithm

doi: 10.7717/peerj.15647

Figure Lengend Snippet: (A) The predicted value of SGLT1 and SGLT2 expression levels on adrenal edema was 0.9596 with a mean error of 3.31% by the SVM method. (B) The predicted value of SGLT1/2 expression levels for broken reticular fibers was 0.8246 with a mean error of 1.81%. (C) The predicted value of SGLT1/2 expression levels for glycogen content was 0.9478 with a mean error of 2.15%.

Article Snippet: Transverse sections of adrenal glands were incubated with SGLT1 Polyclonal Antibody (1:200, bs-1128R; BIOSS, Beijing, China) and SGLT2 Polyclonal Antibody (1:200, 24654-1-AP; Proteintech, Rosemont, IL, USA).

Techniques: Expressing

Journal: PeerJ

Article Title: Adrenal SGLT1 or SGLT2 as predictors of atherosclerosis under chronic stress based on a computer algorithm

doi: 10.7717/peerj.15647

Figure Lengend Snippet: (A–B) Best training score of 0.018092 at epoch 2999 and relativity of 0.96057 of BP-neural network for predicting adrenal edema from SGLT1 and SGLT2 expression levels after training. (C–D) Validation of the predicted values with the original values revealed only small differences. (E–F) A high-risk warning indicator for adrenal edema derived from SGLT1 and SGLT2 expression levels was found by an interpolation algorithm and presented with a three-dimensional (3D) stereogram of the warning range.

Article Snippet: Transverse sections of adrenal glands were incubated with SGLT1 Polyclonal Antibody (1:200, bs-1128R; BIOSS, Beijing, China) and SGLT2 Polyclonal Antibody (1:200, 24654-1-AP; Proteintech, Rosemont, IL, USA).

Techniques: Expressing, Derivative Assay

Journal: PeerJ

Article Title: Adrenal SGLT1 or SGLT2 as predictors of atherosclerosis under chronic stress based on a computer algorithm

doi: 10.7717/peerj.15647

Figure Lengend Snippet: (A–B) The optimal training score for SGLT1 and SGLT2 to predict adrenal reticular fiber breakage was 0.01571 at epoch 3000 with relativity of 0.96383. (C–D) The predicted data were verified against the original values and a significant difference was found between the two. (E–F) High-risk warning indicators for adrenal reticular fiber breakage were determined from SGLT1 and SGLT2 expression levels, and the warning range was presented with a 3D stereogram.

Article Snippet: Transverse sections of adrenal glands were incubated with SGLT1 Polyclonal Antibody (1:200, bs-1128R; BIOSS, Beijing, China) and SGLT2 Polyclonal Antibody (1:200, 24654-1-AP; Proteintech, Rosemont, IL, USA).

Techniques: Expressing

Journal: PeerJ

Article Title: Adrenal SGLT1 or SGLT2 as predictors of atherosclerosis under chronic stress based on a computer algorithm

doi: 10.7717/peerj.15647

Figure Lengend Snippet: (A–B) The best training score was 0.013784 at epoch 3000 with a relativity of 0.97073. (C–D) Validation revealed only small differences between the predicted values and the original values. (E–F) High-risk warning indicators for adrenal gland glycogen content were determined from SGLT1 and SGLT2 expression levels, and the warning range was presented with a 3D stereogram.

Article Snippet: Transverse sections of adrenal glands were incubated with SGLT1 Polyclonal Antibody (1:200, bs-1128R; BIOSS, Beijing, China) and SGLT2 Polyclonal Antibody (1:200, 24654-1-AP; Proteintech, Rosemont, IL, USA).

Techniques: Expressing

Journal: PeerJ

Article Title: Adrenal SGLT1 or SGLT2 as predictors of atherosclerosis under chronic stress based on a computer algorithm

doi: 10.7717/peerj.15647

Figure Lengend Snippet: (A–B) Rose-colored SGLT1 fluorescence and pink-colored SGLT2 fluorescence could be seen in the adrenal cross sections of the four groups. (C) Semi-quantitative analysis of SGLT1 and SGLT2 expression in the adrenal glands of four groups showed that SGLT1 and SGLT2 expression levels were higher in mice after stress than in the corresponding non-stressed mice. (D) Expression of SGLT1 and SGLT2 was determined by Western blot analysis. (E) mRNA expression of SGLT1 and SGLT2 was detected by qPCR.

Article Snippet: Paraffin sections of the adrenal glands were stained with SGLT1 antibody (1:200, BIOSS, Beijing, China) and SGLT2 antibody (1:200, Proteintech, Rosemont, USA), and incubated with horseradish peroxidase (HRP) secondary antibodies.

Techniques: Fluorescence, Expressing, Western Blot

Journal: PeerJ

Article Title: Adrenal SGLT1 or SGLT2 as predictors of atherosclerosis under chronic stress based on a computer algorithm

doi: 10.7717/peerj.15647

Figure Lengend Snippet: CYP11B2 labeled in red showed the adrenal cortex region, while tyrosine hydroxylase (TH) labeled in green showed the adrenal medulla. The nucleus was stained blue by DAPI. SGLT1 was detected by a rose and SGLT2 by a pink. The distribution of SGLT1 and SGLT2 in the adrenal glands of mice in the CON, CON+CS, HF+Apoe -/- , and HF+Apoe -/- +CS groups was observed to be altered by chronic stress.

Article Snippet: Paraffin sections of the adrenal glands were stained with SGLT1 antibody (1:200, BIOSS, Beijing, China) and SGLT2 antibody (1:200, Proteintech, Rosemont, USA), and incubated with horseradish peroxidase (HRP) secondary antibodies.

Techniques: Labeling, Staining

Journal: PeerJ

Article Title: Adrenal SGLT1 or SGLT2 as predictors of atherosclerosis under chronic stress based on a computer algorithm

doi: 10.7717/peerj.15647

Figure Lengend Snippet: (A) The distribution of SGLT1 was observed under low and high magnification, and the differences in SGLT1 expression level in the adrenal gland of mice in the four groups were analyzed by semi-quantitative methods. (B) The distribution of SGLT2 was observed under high and low magnification in the adrenal gland, and the differences in expression between the four groups were analyzed. (C) Edema, glycogen content, and reticulocyte fiber breakage were positively correlated with the expression levels of SGLT1 and SGLT2 in the adrenal gland, respectively.* P < 0.05, ** P < 0.01, *** P < 0.001; ns =no significance.

Article Snippet: Paraffin sections of the adrenal glands were stained with SGLT1 antibody (1:200, BIOSS, Beijing, China) and SGLT2 antibody (1:200, Proteintech, Rosemont, USA), and incubated with horseradish peroxidase (HRP) secondary antibodies.

Techniques: Expressing

Journal: PeerJ

Article Title: Adrenal SGLT1 or SGLT2 as predictors of atherosclerosis under chronic stress based on a computer algorithm

doi: 10.7717/peerj.15647

Figure Lengend Snippet: (A) Best training score of 0.0096775 with an epoch of 3000 after BP-neural network training for predicting intima-media thickness of abdominal aorta from SGLT1 and SGLT2 expression levels. (B) Relativity of 0.9841 with good correlation between input and output quantities. (C–D) Validation of the predicted data and the original values, with only small differences. (E) Best training score of 0.012396 with an epoch of 3000 after training the BP-neural network for predicting the internal diameter of abdominal aorta from SGLT1 and SGLT2 expression levels. (F) Relativity of 0.9805, with a good correlation between input and output volumes. (G–H) Validation of predicted data and original values.

Article Snippet: Paraffin sections of the adrenal glands were stained with SGLT1 antibody (1:200, BIOSS, Beijing, China) and SGLT2 antibody (1:200, Proteintech, Rosemont, USA), and incubated with horseradish peroxidase (HRP) secondary antibodies.

Techniques: Expressing

Journal: PeerJ

Article Title: Adrenal SGLT1 or SGLT2 as predictors of atherosclerosis under chronic stress based on a computer algorithm

doi: 10.7717/peerj.15647

Figure Lengend Snippet: (A) The ROC curves showed that the expression level of SGLT1 in the adrenal gland sensitively and specifically predicted adrenal edema, broken reticular fibers, and glycogen content. (B) The ROC curves showed that the expression level of SGLT2 in the adrenal gland sensitively and specifically predicted adrenal edema, broken reticular fibers, and glycogen content.

Article Snippet: Paraffin sections of the adrenal glands were stained with SGLT1 antibody (1:200, BIOSS, Beijing, China) and SGLT2 antibody (1:200, Proteintech, Rosemont, USA), and incubated with horseradish peroxidase (HRP) secondary antibodies.

Techniques: Expressing

Journal: PeerJ

Article Title: Adrenal SGLT1 or SGLT2 as predictors of atherosclerosis under chronic stress based on a computer algorithm

doi: 10.7717/peerj.15647

Figure Lengend Snippet: (A) The predicted value of SGLT1 and SGLT2 expression levels on adrenal edema was 0.9596 with a mean error of 3.31% by the SVM method. (B) The predicted value of SGLT1/2 expression levels for broken reticular fibers was 0.8246 with a mean error of 1.81%. (C) The predicted value of SGLT1/2 expression levels for glycogen content was 0.9478 with a mean error of 2.15%.

Article Snippet: Paraffin sections of the adrenal glands were stained with SGLT1 antibody (1:200, BIOSS, Beijing, China) and SGLT2 antibody (1:200, Proteintech, Rosemont, USA), and incubated with horseradish peroxidase (HRP) secondary antibodies.

Techniques: Expressing

Journal: PeerJ

Article Title: Adrenal SGLT1 or SGLT2 as predictors of atherosclerosis under chronic stress based on a computer algorithm

doi: 10.7717/peerj.15647

Figure Lengend Snippet: (A–B) Best training score of 0.018092 at epoch 2999 and relativity of 0.96057 of BP-neural network for predicting adrenal edema from SGLT1 and SGLT2 expression levels after training. (C–D) Validation of the predicted values with the original values revealed only small differences. (E–F) A high-risk warning indicator for adrenal edema derived from SGLT1 and SGLT2 expression levels was found by an interpolation algorithm and presented with a three-dimensional (3D) stereogram of the warning range.

Article Snippet: Paraffin sections of the adrenal glands were stained with SGLT1 antibody (1:200, BIOSS, Beijing, China) and SGLT2 antibody (1:200, Proteintech, Rosemont, USA), and incubated with horseradish peroxidase (HRP) secondary antibodies.

Techniques: Expressing, Derivative Assay

Journal: PeerJ

Article Title: Adrenal SGLT1 or SGLT2 as predictors of atherosclerosis under chronic stress based on a computer algorithm

doi: 10.7717/peerj.15647

Figure Lengend Snippet: (A–B) The optimal training score for SGLT1 and SGLT2 to predict adrenal reticular fiber breakage was 0.01571 at epoch 3000 with relativity of 0.96383. (C–D) The predicted data were verified against the original values and a significant difference was found between the two. (E–F) High-risk warning indicators for adrenal reticular fiber breakage were determined from SGLT1 and SGLT2 expression levels, and the warning range was presented with a 3D stereogram.

Article Snippet: Paraffin sections of the adrenal glands were stained with SGLT1 antibody (1:200, BIOSS, Beijing, China) and SGLT2 antibody (1:200, Proteintech, Rosemont, USA), and incubated with horseradish peroxidase (HRP) secondary antibodies.

Techniques: Expressing

Journal: PeerJ

Article Title: Adrenal SGLT1 or SGLT2 as predictors of atherosclerosis under chronic stress based on a computer algorithm

doi: 10.7717/peerj.15647

Figure Lengend Snippet: (A–B) The best training score was 0.013784 at epoch 3000 with a relativity of 0.97073. (C–D) Validation revealed only small differences between the predicted values and the original values. (E–F) High-risk warning indicators for adrenal gland glycogen content were determined from SGLT1 and SGLT2 expression levels, and the warning range was presented with a 3D stereogram.

Article Snippet: Paraffin sections of the adrenal glands were stained with SGLT1 antibody (1:200, BIOSS, Beijing, China) and SGLT2 antibody (1:200, Proteintech, Rosemont, USA), and incubated with horseradish peroxidase (HRP) secondary antibodies.

Techniques: Expressing

Journal: Veterinary Research

Article Title: Cryptosporidium parvum competes with the intestinal epithelial cells for glucose and impairs systemic glucose supply in neonatal calves

doi: 10.1186/s13567-023-01172-y

Figure Lengend Snippet: Antibodies used for Western blot

Article Snippet: Rabbit-anti-SGLT1 , 1:1000 , Bioss Antibodies/#bs-1128R-TR.

Techniques: Western Blot

Journal: Veterinary Research

Article Title: Cryptosporidium parvum competes with the intestinal epithelial cells for glucose and impairs systemic glucose supply in neonatal calves

doi: 10.1186/s13567-023-01172-y

Figure Lengend Snippet: Primers used for RT-qPCR

Article Snippet: Rabbit-anti-SGLT1 , 1:1000 , Bioss Antibodies/#bs-1128R-TR.

Techniques: Sequencing, Amplification

Journal: Veterinary Research

Article Title: Cryptosporidium parvum competes with the intestinal epithelial cells for glucose and impairs systemic glucose supply in neonatal calves

doi: 10.1186/s13567-023-01172-y

Figure Lengend Snippet: Antibodies used for Western blot

Article Snippet: Rabbit-anti-SGLT1 , 1:1000 , Bioss Antibodies/#bs-1128R-TR.

Techniques: Western Blot

Journal: Veterinary Research

Article Title: Cryptosporidium parvum competes with the intestinal epithelial cells for glucose and impairs systemic glucose supply in neonatal calves

doi: 10.1186/s13567-023-01172-y

Figure Lengend Snippet: Antibodies used for IF staining

Article Snippet: Rabbit-anti-SGLT1 , 1:1000 , Bioss Antibodies/#bs-1128R-TR.

Techniques:

Journal: Veterinary Research

Article Title: Cryptosporidium parvum competes with the intestinal epithelial cells for glucose and impairs systemic glucose supply in neonatal calves

doi: 10.1186/s13567-023-01172-y

Figure Lengend Snippet: Protein levels of SGLT1 and GLUT2 in epithelia from control (gray boxes) and infected calves (red boxes). A shows the protein abundance in total protein extracts (P1) while B displays the protein levels in BBM preparations (P2) in relation to P1, i.e., the apical enrichment of each protein. Enrichment of VILLIN was calculated to verify the BBM preparation. Boxes show median and percentiles plus error bars, N = 4 (control) and 5 (infection); Student’s t -test. C shows representative blots.

Article Snippet: Rabbit-anti-SGLT1 , 1:1000 , Bioss Antibodies/#bs-1128R-TR.

Techniques: Infection, Protein Enrichment

Journal: Veterinary Research

Article Title: Cryptosporidium parvum competes with the intestinal epithelial cells for glucose and impairs systemic glucose supply in neonatal calves

doi: 10.1186/s13567-023-01172-y

Figure Lengend Snippet: Immunofluorescent staining of SGLT1 (A, B, green) and GLUT2 (C, D, green) in epithelia from control (A, C) and infected (B, D) animals. White arrows indicate parasite stages attached to the epithelial cells (red staining at the outside of the villi, unspecific staining of erythrocytes inside the villi). Cell nuclei are counterstained in blue. The insert shows a control stained with secondary antibody only. Scale bars: 20 μm.

Article Snippet: Rabbit-anti-SGLT1 , 1:1000 , Bioss Antibodies/#bs-1128R-TR.

Techniques: Staining, Infection

Journal: Foods

Article Title: Methylation, Hydroxylation, Glycosylation and Acylation Affect the Transport of Wine Anthocyanins in Caco-2 Cells

doi: 10.3390/foods11233793

Figure Lengend Snippet: Effects of anthocyanins on mRNA and the expression of SGLT1 and GLUT2 during transport. ( a ) mRNA relative expression level of SGLT1; ( b ) relative protein expression level of SGLT1; ( c ) mRNA relative expression level of GLUT2; ( d ) relative protein expression level of GLUT2; ( e ) Western blotting of glucose transporter protein and internal reference protein; ( f ) Western blotting of GLUT2 and internal reference protein; ( g ) effects of anthocyanin pretreatment on GLUT2 mRNA; ( h ) effects of anthocyanin pretreatment on the relative protein expression of GLUT2. Different small letters indicate significant difference at p < 0.05 in each figure. CK: the control group; MS: monoglycoside standard; DS: diglycoside standard; SM: standard mixture of monoglycoside and diglycoside; DM: diglycoside mixture; MM: monoglycoside mixture; 1d: Mv-3-glc standard pretreatment for 1 day; 3d: Mv-3-glc standard pretreatment for 3 days; 7d: Mv-3-glc standard pretreatment for 7days.

Article Snippet: Rabbit polyclonal antibodies (Anti-SGLT1 and Anti-GLUT2) were obtained from Bioss Biological Technology Co., Ltd. (Beijing, China).

Techniques: Expressing, Western Blot