κ casein  (Bioss)

Journal: Toxins

Article Title: PGN and LTA from Staphylococcus aureus Induced Inflammation and Decreased Lactation through Regulating DNA Methylation and Histone H3 Acetylation in Bovine Mammary Epithelial Cells

doi: 10.3390/toxins12040238

Figure Lengend Snippet: RT-qPCR validation of the differentially expressed genes (DEGs) obtained by RNA sequencing (RNA-Seq). ( A ) The genes involved in inflammation. ( B ) The genes involved in casein synthesis. Data represent the mean and standard deviation ( n = 6), and the asterisk indicates statistical difference (* p < 0.05) between the indicated columns, based on one-way analysis of variance and Duncan’s range test. IL-1β , interleukin-1β; IL-6 , interleukin-6; IL-8 , interleukin-8; TNF-α , tumor necrosis factor-α; CXCL1 , chemokine (C-X-C motif) ligand 1; CXCL6 , chemokine (C-X-C motif) ligand 6; CSN1S1 , αS1-casein; CSN2 , β-casein; CSN3 , κ-casein; RT-qPCR, reverse transcription quantitative real-time polymerase chain reaction; CON, control group; PGN, peptidoglycan group; LTA, lipoteichoic acid group; MIX, PGN + LTA group.

Article Snippet: The primary antibodies included CSN1S1 (Bioss Antibodies, bs-10034R, Beijing, China), CSN2 (Bioss Antibodies, bs-10032R, Beijing, China), CSN3 (Bioss Antibodies, bs-10031R, Beijing, China), Histone H3 (17168-1-AP, Proteintech, Wuhan, China), Histone H4 (16047-1-AP, Proteintech, Wuhan, China), acetylated Histone H3 (Acetyl Lys9/14, Bioss Antibodies, bs-4316R, Beijing, China), acetylated Histone H4 (Acetyl K5, Bioss Antibodies, bs-10721R, Beijing, China), and GAPDH (60004-1-Ig, Proteintech, Wuhan, China).

Techniques: Quantitative RT-PCR, RNA Sequencing Assay, Standard Deviation, Real-time Polymerase Chain Reaction

Journal: Toxins

Article Title: PGN and LTA from Staphylococcus aureus Induced Inflammation and Decreased Lactation through Regulating DNA Methylation and Histone H3 Acetylation in Bovine Mammary Epithelial Cells

doi: 10.3390/toxins12040238

Figure Lengend Snippet: The protein expression of the three caseins (CSN1S1, CSN2, and CSN3). The total protein was isolated to examine casein expression by Western Blot analysis, and the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was selected as a housekeeping protein. Quantitation of blots is representative of three independent experiments. Data represent the mean and standard deviation ( n = 6), and the asterisk indicates statistical difference (* p < 0.05) between the indicated columns, based on one-way analysis of variance and Duncan’s range test. CSN1S1, αS1-casein; CSN2, β-casein; CSN3, κ-casein; CON, control group; PGN, peptidoglycan group; LTA, lipoteichoic acid group; MIX, PGN + LTA group.

Article Snippet: The primary antibodies included CSN1S1 (Bioss Antibodies, bs-10034R, Beijing, China), CSN2 (Bioss Antibodies, bs-10032R, Beijing, China), CSN3 (Bioss Antibodies, bs-10031R, Beijing, China), Histone H3 (17168-1-AP, Proteintech, Wuhan, China), Histone H4 (16047-1-AP, Proteintech, Wuhan, China), acetylated Histone H3 (Acetyl Lys9/14, Bioss Antibodies, bs-4316R, Beijing, China), acetylated Histone H4 (Acetyl K5, Bioss Antibodies, bs-10721R, Beijing, China), and GAPDH (60004-1-Ig, Proteintech, Wuhan, China).

Techniques: Expressing, Isolation, Western Blot, Protein Quantitation, Standard Deviation