κ casein  (Bioss)

Journal: Veterinary and Animal Science

Article Title: Comparative analysis of κ-casein expressions and dairy herd improvement indices in Chinese Holstein cows of diverse κ-casein genotypes and transcripts

doi: 10.1016/j.vas.2024.100421

Figure Lengend Snippet: Schematic diagram of HinfI enzyme digestion for genotype analysis. The A allele had two restriction sites on the DNA single strand, whereas the B allele had one restriction site on the DNA single strand. After HinfI enzyme digestion, three fragments were produced from the AA genotype of CSN3 , two from BB and four from AB.

Article Snippet: The CSN3 CDS reference sequence (ENSBTAT0000028685.5) downloaded from Ensemble and the CSN3 CDS obtained from sequencing were translated into an amino acid sequence by DNAStar.

Techniques: Produced

Journal: Veterinary and Animal Science

Article Title: Comparative analysis of κ-casein expressions and dairy herd improvement indices in Chinese Holstein cows of diverse κ-casein genotypes and transcripts

doi: 10.1016/j.vas.2024.100421

Figure Lengend Snippet: Base and amino acid mutations of the CSN3 CDS region.

Article Snippet: The CSN3 CDS reference sequence (ENSBTAT0000028685.5) downloaded from Ensemble and the CSN3 CDS obtained from sequencing were translated into an amino acid sequence by DNAStar.

Techniques: Variant Assay, Mutagenesis

Journal: Veterinary and Animal Science

Article Title: Comparative analysis of κ-casein expressions and dairy herd improvement indices in Chinese Holstein cows of diverse κ-casein genotypes and transcripts

doi: 10.1016/j.vas.2024.100421

Figure Lengend Snippet: Frequencies of genotypes and alleles and the transcripts of CSN3.

Article Snippet: The CSN3 CDS reference sequence (ENSBTAT0000028685.5) downloaded from Ensemble and the CSN3 CDS obtained from sequencing were translated into an amino acid sequence by DNAStar.

Techniques:

Journal: Veterinary and Animal Science

Article Title: Comparative analysis of κ-casein expressions and dairy herd improvement indices in Chinese Holstein cows of diverse κ-casein genotypes and transcripts

doi: 10.1016/j.vas.2024.100421

Figure Lengend Snippet: Differences in the expression of κ-CN among different the CSN3 genotypes and transcripts by Western blot analysis. On the left of the panels (A1, B1, C1, and D1) are the bands of the Western blot for κ-CN, and on the right (A2, B2, C2, and D2) are the results of grayscale analysis of the bands. Figure A shows a comparison of the κ-CN genotype BB (transcript B) with the genotype AA (transcript A). Figure B shows a comparison of the κ-CN genotype BB (transcript B) with the genotype AB (transcript B). Figure C shows the comparison of the κ-CN genotype AA (transcript A) with the genotype AB (transcript A). Figure D shows the comparison of the κ-CN genotype AB (transcript B) with the genotype AB (transcript A).

Article Snippet: The CSN3 CDS reference sequence (ENSBTAT0000028685.5) downloaded from Ensemble and the CSN3 CDS obtained from sequencing were translated into an amino acid sequence by DNAStar.

Techniques: Expressing, Western Blot, Comparison

Journal: Veterinary and Animal Science

Article Title: Comparative analysis of κ-casein expressions and dairy herd improvement indices in Chinese Holstein cows of diverse κ-casein genotypes and transcripts

doi: 10.1016/j.vas.2024.100421

Figure Lengend Snippet: PCA of the DHI for the different CSN3 genotypes and transcripts. Figure A represents PCA for all DHI indices of all 50 samples, where A1 is the loading plot of the DHI indices, A2 is the PCA score plot for the different κ-CN genotypes, and A3 is the PCA score plot of the different κ-CN transcripts. The following diagram has the same classification. Figure B represents PCA for all DHI indices of 24 mid-lactation samples. Figure C represents PCA for part of the DHI indices of 24 mid-lactation samples. The numbers 1, 2, 3, and 4 in C2 and C3 represent quadrants 1, 2, 3, and 4, respectively.

Article Snippet: The CSN3 CDS reference sequence (ENSBTAT0000028685.5) downloaded from Ensemble and the CSN3 CDS obtained from sequencing were translated into an amino acid sequence by DNAStar.

Techniques:

Journal: Animals : an Open Access Journal from MDPI

Article Title: Polymorphism of the CSN3 3’UTR in Dairy Cows Causes Changes in bta-miR-708 Binding Ability and κ-Casein Expression

doi: 10.3390/ani14233462

Figure Lengend Snippet: The mutation site, mutation frequency, and the haplotype of CSN3 3’UTR in 50 cattle.

Article Snippet: After the membranes were blocked with a Protein-Free Rapid Blocking Buffer (PS108P, Epizyme, Shanghai, China) for 10 min, they were incubated with primary antibodies against CSN3 (1:5000, abx130922, Abbexa, Cambridge, UK) and GAPDH (1:2000, AF2819, Beyotime, Shanghai, China) at 4 °C overnight.

Techniques: Mutagenesis

Journal: Animals : an Open Access Journal from MDPI

Article Title: Polymorphism of the CSN3 3’UTR in Dairy Cows Causes Changes in bta-miR-708 Binding Ability and κ-Casein Expression

doi: 10.3390/ani14233462

Figure Lengend Snippet: The relative quantitative analysis of the four casein genes in the Haplotype 1 group and the Haplotype 10 group.

Article Snippet: After the membranes were blocked with a Protein-Free Rapid Blocking Buffer (PS108P, Epizyme, Shanghai, China) for 10 min, they were incubated with primary antibodies against CSN3 (1:5000, abx130922, Abbexa, Cambridge, UK) and GAPDH (1:2000, AF2819, Beyotime, Shanghai, China) at 4 °C overnight.

Techniques:

Journal: Animals : an Open Access Journal from MDPI

Article Title: Polymorphism of the CSN3 3’UTR in Dairy Cows Causes Changes in bta-miR-708 Binding Ability and κ-Casein Expression

doi: 10.3390/ani14233462

Figure Lengend Snippet: The effect of CSN3 3’UTR polymorphisms on CSN3 protein expression. ( A ) CSN3 protein expression was assessed by Western blotting. Samples 1-3 were from the Haplotype 1 group (0-mutations), and samples 4-6 were from the Haplotype 10 group (7-mutations). ( B ) Grayscale analysis of CSN3 protein expression. CSN3 gray-level ratio = CSN3 grayscale value/GAPDH grayscale value. The statistical differences between two groups were analyzed using a t -test.

Article Snippet: After the membranes were blocked with a Protein-Free Rapid Blocking Buffer (PS108P, Epizyme, Shanghai, China) for 10 min, they were incubated with primary antibodies against CSN3 (1:5000, abx130922, Abbexa, Cambridge, UK) and GAPDH (1:2000, AF2819, Beyotime, Shanghai, China) at 4 °C overnight.

Techniques: Expressing, Western Blot

Journal: Animals : an Open Access Journal from MDPI

Article Title: Polymorphism of the CSN3 3’UTR in Dairy Cows Causes Changes in bta-miR-708 Binding Ability and κ-Casein Expression

doi: 10.3390/ani14233462

Figure Lengend Snippet: The possible binding sites between the seed sequences of the six miRNAs and the CSN3 3’ UTR. Different colors correspond to different miRNA binding sites. The reference sequence is obtained from NCBI (NM_174294.2) and belongs to the Haplotype 10 group (7-mutations). Bases with a bright yellow background are mutated bases. The underlined base is the corresponding binding site in the seed region of miRNA.

Article Snippet: After the membranes were blocked with a Protein-Free Rapid Blocking Buffer (PS108P, Epizyme, Shanghai, China) for 10 min, they were incubated with primary antibodies against CSN3 (1:5000, abx130922, Abbexa, Cambridge, UK) and GAPDH (1:2000, AF2819, Beyotime, Shanghai, China) at 4 °C overnight.

Techniques: Binding Assay, Sequencing

Journal: Animals : an Open Access Journal from MDPI

Article Title: Polymorphism of the CSN3 3’UTR in Dairy Cows Causes Changes in bta-miR-708 Binding Ability and κ-Casein Expression

doi: 10.3390/ani14233462

Figure Lengend Snippet: The dual-fluorescence assay results for six miRNAs and two recombinant vectors containing the psi-checkII-CSN3 3’ UTR (Haplotype 1 and Haplotype 10). Psi-checkII- CSN3 3’ UTR-1 was the recombinant vector with Haplotype 1. Psi-checkII- CSN3 3’ UTR-2 was the recombinant vector with Haplotype 10. The left panel shows the results for MAC-T cells, and the right panel shows the results for 293T cells. ( A ) represents the co-transfection of Psi-checkII-CSN3 3’UTR-1 and six miRNAs into MAC-T cells. ( B ) represents the co-transfection of Psi-checkII-CSN3 3’UTR-1 and six miRNAs into 293T cells. ( C ) represents the co-transfection of Psi-checkII-CSN3 3’UTR-2 and six miRNAs into MAC-T cells. ( D ) represents the co-transfection of Psi-checkII-CSN3 3’UTR-2 and six miRNAs into 293T cells. * indicates a significant difference between the two groups, i.e., p < 0.05.

Article Snippet: After the membranes were blocked with a Protein-Free Rapid Blocking Buffer (PS108P, Epizyme, Shanghai, China) for 10 min, they were incubated with primary antibodies against CSN3 (1:5000, abx130922, Abbexa, Cambridge, UK) and GAPDH (1:2000, AF2819, Beyotime, Shanghai, China) at 4 °C overnight.

Techniques: Fluorescence, Recombinant, Plasmid Preparation, Cotransfection