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brca2  (Proteintech)


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    Proteintech brca2
    (A) . U2OS or Hela cells were transfected with Control (siCON), MCPH1 (siMCPH1) or <t>BRCA2</t> (siBRCA2) siRNA for 48h or 120h and protein expression analysed via Western blotting with the indicated antibodies. MCPH1 expression in sgCON (control) and sgMCPH1 (MCPH1 knockout) cells was also investigated by Western blotting. (B) Quantification of MCPH1 or BRCA2 protein bands normalised to GAPDH and expressed as % change relative to siCON or sgCON. Data is the mean and standard error of three (48h) or two (120h and sgCON/sgMCPH1) independent experiments. (C) U2OS or Hela cells were transfected with MCPH1 siRNA for 72h before analysis of nuclear morphology (premature chromosome condensation, PCC) via DAPI staining and fluorescence microscopy. (D) The % of cells displaying PCC, with at least 100 cells analysed.
    Brca2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/brca2/BRCA2+Antibody/pmc13048371-64-35-37
    Average 93 stars, based on 42 article reviews
    brca2 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Differential sensitivity of MCPH1- and BRCA2-deficient cancer cells to PARP-1 inhibition"

    Article Title: Differential sensitivity of MCPH1- and BRCA2-deficient cancer cells to PARP-1 inhibition

    Journal: PLOS One

    doi: 10.1371/journal.pone.0345514

    (A) . U2OS or Hela cells were transfected with Control (siCON), MCPH1 (siMCPH1) or BRCA2 (siBRCA2) siRNA for 48h or 120h and protein expression analysed via Western blotting with the indicated antibodies. MCPH1 expression in sgCON (control) and sgMCPH1 (MCPH1 knockout) cells was also investigated by Western blotting. (B) Quantification of MCPH1 or BRCA2 protein bands normalised to GAPDH and expressed as % change relative to siCON or sgCON. Data is the mean and standard error of three (48h) or two (120h and sgCON/sgMCPH1) independent experiments. (C) U2OS or Hela cells were transfected with MCPH1 siRNA for 72h before analysis of nuclear morphology (premature chromosome condensation, PCC) via DAPI staining and fluorescence microscopy. (D) The % of cells displaying PCC, with at least 100 cells analysed.
    Figure Legend Snippet: (A) . U2OS or Hela cells were transfected with Control (siCON), MCPH1 (siMCPH1) or BRCA2 (siBRCA2) siRNA for 48h or 120h and protein expression analysed via Western blotting with the indicated antibodies. MCPH1 expression in sgCON (control) and sgMCPH1 (MCPH1 knockout) cells was also investigated by Western blotting. (B) Quantification of MCPH1 or BRCA2 protein bands normalised to GAPDH and expressed as % change relative to siCON or sgCON. Data is the mean and standard error of three (48h) or two (120h and sgCON/sgMCPH1) independent experiments. (C) U2OS or Hela cells were transfected with MCPH1 siRNA for 72h before analysis of nuclear morphology (premature chromosome condensation, PCC) via DAPI staining and fluorescence microscopy. (D) The % of cells displaying PCC, with at least 100 cells analysed.

    Techniques Used: Transfection, Control, Expressing, Western Blot, Knock-Out, Staining, Fluorescence, Microscopy

    U2OS cells were transfected with control (siCON), BRCA2 (siBRCA2) or BRCA2 + MCPH1 (siBRCA2 + siMCPH1) siRNA for 48h before (A) Western blot analysis or (B) seeding into 96-well plates and treating with the indicated doses of AZD2461 or Talazoparib, with each treatment performed in triplicate. After 96-hours, cell viability was measured using an MTS assay. MTS data represents the mean and standard error from at least three independent experiments, with cell viability expressed as a % relative to the corresponding untreated sample. Statistical significance was measured using two-way ANOVA (p-values described in text, with significance set at p < 0.05).
    Figure Legend Snippet: U2OS cells were transfected with control (siCON), BRCA2 (siBRCA2) or BRCA2 + MCPH1 (siBRCA2 + siMCPH1) siRNA for 48h before (A) Western blot analysis or (B) seeding into 96-well plates and treating with the indicated doses of AZD2461 or Talazoparib, with each treatment performed in triplicate. After 96-hours, cell viability was measured using an MTS assay. MTS data represents the mean and standard error from at least three independent experiments, with cell viability expressed as a % relative to the corresponding untreated sample. Statistical significance was measured using two-way ANOVA (p-values described in text, with significance set at p < 0.05).

    Techniques Used: Transfection, Control, Western Blot, MTS Assay

    Related Articles

    Proximity Ligation Assay:

    Article Title: The Ski2 helicase ASCC3 unwinds DNA upon fork stalling to control replication stress responses
    Article Snippet: Drugs used were aphidicolin (Sigma); camptothecin (Sigma); hydroxyurea (BioShop Canada); RAD52 inhibitor (AICAR, Cayman Chemical); REV1 inhibitor JH-RE-06 (SML2993, Sigma); RO-3306 (Selleck Chemicals). .. Antibodies used include: ASCC2 (1:1000; A304-020A, Bethyl Laboratories); ASCC2 (1:5000; 1152-1-AP, Proteintech); ASCC3 (1:400; 17627-1-AP, Proteintech); ASCC3 (1:1000; PA5-56794, Invitrogen); Biotin (1:100000; A150-109A, Bethyl Laboratory); Biotin (1:100000; 200-002-211, Jackson ImmunoResearch); BrdU (1:100; 347580, BD Biosciences); BrdU (1:500; MAB3222, Millipore); BrdU (BU1/75 [ICR1]) (1:800; NB500-169, Novus Biologicals); 53BP1 (1:2000; 612522, BD Biosciences); BRCA1 (1:5000; 07-434, Millipore); BRCA2 (1:2000; 29450-1-AP, Proteintech); CHK1 (1:250; sc-7898, Santa cruz); CHK1-pS345 (1:1000; 2348S, Cell Signaling); CSB (1:200; ab66598, Abcam); FASN (1:20000; 10624-2-AP, Proteintech); GFP (1:1000; 50430-2-AP, Proteintech); HA (1:500; #2367, Cell Signaling); HLTF (1:1000; A300-229A, Bethyl Laboratory); Myc (1:1000; 9E10, Calbiochem); PCNA (1:2000; sc-56, Santa Cruz); PCNA (1:600 for PLA or 1:4000 for western blot; 10205-2-AP, Proteintech); Ubiquityl-PCNA (K164) (1:1000; 13439T, Cell Signaling); PRIMPOL (1:1000; 29824-1-AP, Proteintech); RAD51 (1:2000, ab63801, Abcam); RFWD3 (1:1000; 19893-1-AP, Proteintech); RPA32 (1:10000; NB100-332, Novus Biologicals); RPA32 (1:200; ab2175, Abcam); RPA32-pS33 (1:50000; A300-246A, Bethyl Laboratories); RPA70 (1:2000; 2267S, Cell Signaling); SHPRH (21995-1-AP, Proteintech); SMARCAL1 (1:100, sc-166209, Santa cruz); SMARCAL1 (1:2000; GTX109468, GenTex); TRIP4 (1:1000; 12324-1-AP, Proteintech); α-tubulin (1:20,000; T9026, Sigma); γ-tubulin (1:20,000; GTU88, Sigma). ..

    Western Blot:

    Article Title: The Ski2 helicase ASCC3 unwinds DNA upon fork stalling to control replication stress responses
    Article Snippet: Drugs used were aphidicolin (Sigma); camptothecin (Sigma); hydroxyurea (BioShop Canada); RAD52 inhibitor (AICAR, Cayman Chemical); REV1 inhibitor JH-RE-06 (SML2993, Sigma); RO-3306 (Selleck Chemicals). .. Antibodies used include: ASCC2 (1:1000; A304-020A, Bethyl Laboratories); ASCC2 (1:5000; 1152-1-AP, Proteintech); ASCC3 (1:400; 17627-1-AP, Proteintech); ASCC3 (1:1000; PA5-56794, Invitrogen); Biotin (1:100000; A150-109A, Bethyl Laboratory); Biotin (1:100000; 200-002-211, Jackson ImmunoResearch); BrdU (1:100; 347580, BD Biosciences); BrdU (1:500; MAB3222, Millipore); BrdU (BU1/75 [ICR1]) (1:800; NB500-169, Novus Biologicals); 53BP1 (1:2000; 612522, BD Biosciences); BRCA1 (1:5000; 07-434, Millipore); BRCA2 (1:2000; 29450-1-AP, Proteintech); CHK1 (1:250; sc-7898, Santa cruz); CHK1-pS345 (1:1000; 2348S, Cell Signaling); CSB (1:200; ab66598, Abcam); FASN (1:20000; 10624-2-AP, Proteintech); GFP (1:1000; 50430-2-AP, Proteintech); HA (1:500; #2367, Cell Signaling); HLTF (1:1000; A300-229A, Bethyl Laboratory); Myc (1:1000; 9E10, Calbiochem); PCNA (1:2000; sc-56, Santa Cruz); PCNA (1:600 for PLA or 1:4000 for western blot; 10205-2-AP, Proteintech); Ubiquityl-PCNA (K164) (1:1000; 13439T, Cell Signaling); PRIMPOL (1:1000; 29824-1-AP, Proteintech); RAD51 (1:2000, ab63801, Abcam); RFWD3 (1:1000; 19893-1-AP, Proteintech); RPA32 (1:10000; NB100-332, Novus Biologicals); RPA32 (1:200; ab2175, Abcam); RPA32-pS33 (1:50000; A300-246A, Bethyl Laboratories); RPA70 (1:2000; 2267S, Cell Signaling); SHPRH (21995-1-AP, Proteintech); SMARCAL1 (1:100, sc-166209, Santa cruz); SMARCAL1 (1:2000; GTX109468, GenTex); TRIP4 (1:1000; 12324-1-AP, Proteintech); α-tubulin (1:20,000; T9026, Sigma); γ-tubulin (1:20,000; GTU88, Sigma). ..

    Article Title: CSB and SMARCAL1 compete for RPA32 at stalled forks and differentially control the fate of stalled forks in BRCA2-deficient cells
    Article Snippet: All plasmids were verified by Sanger sequencing. siRNAs used were from Dharmacon: non-targeting siRNA (siControl; D-001206–14-05); siBRCA2 (D-003462–04) ( ); siSMARCAL1 (D-013058–04-0002) ( ); siPRIMPOL (GAGGAAAGCUGGACAUCGA) ( ). .. Antibodies used include: Biotin (1:100000; A150-109A, Bethyl Laboratories); Biotin (1:100000; 200-002-211, Jackson ImmunoResearch); 53BP1 (1:4000; 612522, BD Biosciences); BRCA2 (1:2000; 29450-1-AP, Proteintech); BrdU (1:50; 347580, BD Biosciences); BrdU (BU1/75[ICR1]) (1:400; NB500-169, Novus Biologicals); CSB (1:200; 553C5a, Fitzgerald); CSB (1:1000; A301-347A, Bethyl Laboratories); mCherry (1:10000; NBP2-25157, Novus Biologicals); HA-tag (1:500; #2367, Cell Signaling); MUS81 (1:2000; sc-47692, Santa Cruz); Myc (1:1000 for western, 1:2000 for IF; 9E10, Calbiochem); RPA70 (1:2000; 2267S, Cell Signaling); RPA32 (1:10000; NB100-332, Novus Biologicals); RPA32-pS4/pS8 (1:2000; A300-245A, Bethyl Laboratories); RPA32-pS33 (1:50000; A300-246A, Bethyl Laboratories), PRIMPOL (1:1000; 29824-1-AP, Proteintech); SMARCAL1 (1:50–100; sc-166209, Santa cruz); SMARCAL1 (1:2000; GTX109468, GeneTex); γ-tubulin (1:20000; GTU88, Sigma); α-tubulin (1:10000; T9026, Sigma). .. U2OS (ATCC), U2OS CSB-KO , U2OS-265 CSB-KO , hTERT-RPE CSB-KO , HCT116 (ATCC), HCT116 CSB-KO , HEK293 (ATCC) and Phoenix ( ) cells were grown in DMEM medium with 10% fetal bovine serum supplemented with non-essential amino acids, l -glutamine, 100 U/ml penicillin and 0.1 mg/ml streptomycin.

    Article Title: Inherited deficiency of DIAPH1 identifies a DNA double strand break repair pathway regulated by γ-actin
    Article Snippet: .. If you are not sure if a list item applies to your research, read the appropriate section before selecting a response. ary M arch 2021 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Antibodies used for Western blotting: pS1981 ATM (AF1655, 1:500) from R&D systems; Arp2 (sc-166103, 1:500) and U1-70K (sc-390988, 1:2000) from Santa Cruz Biotechnology; ATM (A300-299A, 1:500), pS824-KAP1 (A300-767A, 1:1000), KAP1 (A300-274A, 1:3000), pS966-SMC1 (A300-050A, 1:1000), SMC1 (A300-055A, 1:1000), Chk2 (A300-681A, 1:1000) and DIAPH1 (A300-077A, 1:500) from Fortis Life Sciences; pT68-CHK2 (2197, 1:500) from Cell Signalling Technology; H2A (07-146, 1:1000); γ-H2AX (05-636, 1:3000), beta-actin (A5316, 1:10000) and HA (H9658, 1:3000) from Sigma-Aldrich; pS343-NBS1 (ab47272, 1:500), Mre11 (ab214, 1:2000), Histone H3 (ab1791, 1:2000) and ARPC4 (ab217065, 1:1000) from Abcam; Nbs1 (GTX70224, 1:10000) from GeneTex; BRCA2 (OP95, 1:500), RPA2 (NA18, 1:1000), gamma-actin (MABT824, 1:10000) and Rad51 (PC130, 1:500) from Merck; Vinculin (66305-1, 1:5000) from Proteintech. .. Antibodies used for immunofluorescence: γ-H2AX (Sigma-Aldrich, 05-636, 1:1000), Rad51 (Sigma-Aldrich, PC130, 1:500), 53BP1 (Novus-Biologicals, NB100-904, 1:1000), RPA2 (Abcam, ab2174, 1:250), BrdU (Abcam, BU1/75 (ICR1), 1:100), Mre11 (Abcam, ab214, 1:1000), BRCA1 (Santa Cruz Biotechnology, sc-6954, 1:200), CENPF (Antibody Atlas, HPA052382, 1:1000), Mitosin (BD Transduction labs, 610768, 1:1000), DIAPH1 (Fortis Life Sciences, A300-077A, 1:250).

    other:

    Article Title: Tumor-derived apolipoprotein E confers resistance to temozolomide in pancreatic neuroendocrine tumors
    Article Snippet: Antibodies were obtained from the following sources, unless indicated otherwise: BRCA1 (Abclonal, A11549 and A11034), BRCA2 (Ablonal, A2435), β-catenin (Proteintech, 51067-2-AP), γH2AX (Abcam, ab81299), and β-actin (Abclonal, AC006).

    Incubation:

    Article Title: Differential sensitivity of MCPH1- and BRCA2-deficient cancer cells to PARP-1 inhibition.
    Article Snippet: Protein electrophoresis was carried out using 3−8% Tris-Acetate or 4−12% bis tris NU-PAGE gels (Invitrogen) prior to transfer to nitrocellulose membrane using transfer stacks and an iBlot2 machine (both Invitrogen). .. Membranes were blocked with 5% dried skimmed milk/TBS-T (50mM Tris pH 7.6, 150mM NaCl and 0.2% v/v Tween-20) for a minimum of 1-hour prior to overnight incubation at 4°C with primary antibodies; MCPH1 (11962–1-AP, Proteintech), BRCA2 (29450–1-AP, Proteintech) and GAPDH (60004–1-Ig, Proteintech). .. Membranes were then washed with TBS-T and incubated with secondary antibody, Anti-rabbit HRP Conjugated (7074S, Cell signalling) for 2 hours at 4°C.

    Article Title: Differential sensitivity of MCPH1- and BRCA2-deficient cancer cells to PARP-1 inhibition
    Article Snippet: Protein electrophoresis was carried out using 3−8% Tris-Acetate or 4−12% bis tris NU-PAGE gels (Invitrogen) prior to transfer to nitrocellulose membrane using transfer stacks and an iBlot2 machine (both Invitrogen). .. Membranes were blocked with 5% dried skimmed milk/TBS-T (50mM Tris pH 7.6, 150mM NaCl and 0.2% v/v Tween-20) for a minimum of 1-hour prior to overnight incubation at 4°C with primary antibodies; MCPH1 (11962–1-AP, Proteintech), BRCA2 (29450–1-AP, Proteintech) and GAPDH (60004–1-Ig, Proteintech). .. Membranes were then washed with TBS-T and incubated with secondary antibody, Anti-rabbit HRP Conjugated (7074S, Cell signalling) for 2 hours at 4°C.

    Chromatin Immunoprecipitation:

    Article Title: Inherited deficiency of DIAPH1 identifies a DNA double strand break repair pathway regulated by γ-actin
    Article Snippet: .. If you are not sure if a list item applies to your research, read the appropriate section before selecting a response. ary M arch 2021 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Antibodies used for Western blotting: pS1981 ATM (AF1655, 1:500) from R&D systems; Arp2 (sc-166103, 1:500) and U1-70K (sc-390988, 1:2000) from Santa Cruz Biotechnology; ATM (A300-299A, 1:500), pS824-KAP1 (A300-767A, 1:1000), KAP1 (A300-274A, 1:3000), pS966-SMC1 (A300-050A, 1:1000), SMC1 (A300-055A, 1:1000), Chk2 (A300-681A, 1:1000) and DIAPH1 (A300-077A, 1:500) from Fortis Life Sciences; pT68-CHK2 (2197, 1:500) from Cell Signalling Technology; H2A (07-146, 1:1000); γ-H2AX (05-636, 1:3000), beta-actin (A5316, 1:10000) and HA (H9658, 1:3000) from Sigma-Aldrich; pS343-NBS1 (ab47272, 1:500), Mre11 (ab214, 1:2000), Histone H3 (ab1791, 1:2000) and ARPC4 (ab217065, 1:1000) from Abcam; Nbs1 (GTX70224, 1:10000) from GeneTex; BRCA2 (OP95, 1:500), RPA2 (NA18, 1:1000), gamma-actin (MABT824, 1:10000) and Rad51 (PC130, 1:500) from Merck; Vinculin (66305-1, 1:5000) from Proteintech. .. Antibodies used for immunofluorescence: γ-H2AX (Sigma-Aldrich, 05-636, 1:1000), Rad51 (Sigma-Aldrich, PC130, 1:500), 53BP1 (Novus-Biologicals, NB100-904, 1:1000), RPA2 (Abcam, ab2174, 1:250), BrdU (Abcam, BU1/75 (ICR1), 1:100), Mre11 (Abcam, ab214, 1:1000), BRCA1 (Santa Cruz Biotechnology, sc-6954, 1:200), CENPF (Antibody Atlas, HPA052382, 1:1000), Mitosin (BD Transduction labs, 610768, 1:1000), DIAPH1 (Fortis Life Sciences, A300-077A, 1:250).

    Flow Cytometry:

    Article Title: Inherited deficiency of DIAPH1 identifies a DNA double strand break repair pathway regulated by γ-actin
    Article Snippet: .. If you are not sure if a list item applies to your research, read the appropriate section before selecting a response. ary M arch 2021 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Antibodies used for Western blotting: pS1981 ATM (AF1655, 1:500) from R&D systems; Arp2 (sc-166103, 1:500) and U1-70K (sc-390988, 1:2000) from Santa Cruz Biotechnology; ATM (A300-299A, 1:500), pS824-KAP1 (A300-767A, 1:1000), KAP1 (A300-274A, 1:3000), pS966-SMC1 (A300-050A, 1:1000), SMC1 (A300-055A, 1:1000), Chk2 (A300-681A, 1:1000) and DIAPH1 (A300-077A, 1:500) from Fortis Life Sciences; pT68-CHK2 (2197, 1:500) from Cell Signalling Technology; H2A (07-146, 1:1000); γ-H2AX (05-636, 1:3000), beta-actin (A5316, 1:10000) and HA (H9658, 1:3000) from Sigma-Aldrich; pS343-NBS1 (ab47272, 1:500), Mre11 (ab214, 1:2000), Histone H3 (ab1791, 1:2000) and ARPC4 (ab217065, 1:1000) from Abcam; Nbs1 (GTX70224, 1:10000) from GeneTex; BRCA2 (OP95, 1:500), RPA2 (NA18, 1:1000), gamma-actin (MABT824, 1:10000) and Rad51 (PC130, 1:500) from Merck; Vinculin (66305-1, 1:5000) from Proteintech. .. Antibodies used for immunofluorescence: γ-H2AX (Sigma-Aldrich, 05-636, 1:1000), Rad51 (Sigma-Aldrich, PC130, 1:500), 53BP1 (Novus-Biologicals, NB100-904, 1:1000), RPA2 (Abcam, ab2174, 1:250), BrdU (Abcam, BU1/75 (ICR1), 1:100), Mre11 (Abcam, ab214, 1:1000), BRCA1 (Santa Cruz Biotechnology, sc-6954, 1:200), CENPF (Antibody Atlas, HPA052382, 1:1000), Mitosin (BD Transduction labs, 610768, 1:1000), DIAPH1 (Fortis Life Sciences, A300-077A, 1:250).

    Magnetic Resonance Imaging:

    Article Title: Inherited deficiency of DIAPH1 identifies a DNA double strand break repair pathway regulated by γ-actin
    Article Snippet: .. If you are not sure if a list item applies to your research, read the appropriate section before selecting a response. ary M arch 2021 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Antibodies used for Western blotting: pS1981 ATM (AF1655, 1:500) from R&D systems; Arp2 (sc-166103, 1:500) and U1-70K (sc-390988, 1:2000) from Santa Cruz Biotechnology; ATM (A300-299A, 1:500), pS824-KAP1 (A300-767A, 1:1000), KAP1 (A300-274A, 1:3000), pS966-SMC1 (A300-050A, 1:1000), SMC1 (A300-055A, 1:1000), Chk2 (A300-681A, 1:1000) and DIAPH1 (A300-077A, 1:500) from Fortis Life Sciences; pT68-CHK2 (2197, 1:500) from Cell Signalling Technology; H2A (07-146, 1:1000); γ-H2AX (05-636, 1:3000), beta-actin (A5316, 1:10000) and HA (H9658, 1:3000) from Sigma-Aldrich; pS343-NBS1 (ab47272, 1:500), Mre11 (ab214, 1:2000), Histone H3 (ab1791, 1:2000) and ARPC4 (ab217065, 1:1000) from Abcam; Nbs1 (GTX70224, 1:10000) from GeneTex; BRCA2 (OP95, 1:500), RPA2 (NA18, 1:1000), gamma-actin (MABT824, 1:10000) and Rad51 (PC130, 1:500) from Merck; Vinculin (66305-1, 1:5000) from Proteintech. .. Antibodies used for immunofluorescence: γ-H2AX (Sigma-Aldrich, 05-636, 1:1000), Rad51 (Sigma-Aldrich, PC130, 1:500), 53BP1 (Novus-Biologicals, NB100-904, 1:1000), RPA2 (Abcam, ab2174, 1:250), BrdU (Abcam, BU1/75 (ICR1), 1:100), Mre11 (Abcam, ab214, 1:1000), BRCA1 (Santa Cruz Biotechnology, sc-6954, 1:200), CENPF (Antibody Atlas, HPA052382, 1:1000), Mitosin (BD Transduction labs, 610768, 1:1000), DIAPH1 (Fortis Life Sciences, A300-077A, 1:250).



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    rabbit anti brca2  (Merck & Co)
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    Merck & Co rabbit anti brca2
    (A) . U2OS or Hela cells were transfected with Control (siCON), MCPH1 (siMCPH1) or <t>BRCA2</t> (siBRCA2) siRNA for 48h or 120h and protein expression analysed via Western blotting with the indicated antibodies. MCPH1 expression in sgCON (control) and sgMCPH1 (MCPH1 knockout) cells was also investigated by Western blotting. (B) Quantification of MCPH1 or BRCA2 protein bands normalised to GAPDH and expressed as % change relative to siCON or sgCON. Data is the mean and standard error of three (48h) or two (120h and sgCON/sgMCPH1) independent experiments. (C) U2OS or Hela cells were transfected with MCPH1 siRNA for 72h before analysis of nuclear morphology (premature chromosome condensation, PCC) via DAPI staining and fluorescence microscopy. (D) The % of cells displaying PCC, with at least 100 cells analysed.
    Rabbit Anti Brca2, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti brca2 antibody  (Cell Signaling Technology Inc)
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    (A) . U2OS or Hela cells were transfected with Control (siCON), MCPH1 (siMCPH1) or <t>BRCA2</t> (siBRCA2) siRNA for 48h or 120h and protein expression analysed via Western blotting with the indicated antibodies. MCPH1 expression in sgCON (control) and sgMCPH1 (MCPH1 knockout) cells was also investigated by Western blotting. (B) Quantification of MCPH1 or BRCA2 protein bands normalised to GAPDH and expressed as % change relative to siCON or sgCON. Data is the mean and standard error of three (48h) or two (120h and sgCON/sgMCPH1) independent experiments. (C) U2OS or Hela cells were transfected with MCPH1 siRNA for 72h before analysis of nuclear morphology (premature chromosome condensation, PCC) via DAPI staining and fluorescence microscopy. (D) The % of cells displaying PCC, with at least 100 cells analysed.
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    brca2  (OriGene)
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    (A) . U2OS or Hela cells were transfected with Control (siCON), MCPH1 (siMCPH1) or <t>BRCA2</t> (siBRCA2) siRNA for 48h or 120h and protein expression analysed via Western blotting with the indicated antibodies. MCPH1 expression in sgCON (control) and sgMCPH1 (MCPH1 knockout) cells was also investigated by Western blotting. (B) Quantification of MCPH1 or BRCA2 protein bands normalised to GAPDH and expressed as % change relative to siCON or sgCON. Data is the mean and standard error of three (48h) or two (120h and sgCON/sgMCPH1) independent experiments. (C) U2OS or Hela cells were transfected with MCPH1 siRNA for 72h before analysis of nuclear morphology (premature chromosome condensation, PCC) via DAPI staining and fluorescence microscopy. (D) The % of cells displaying PCC, with at least 100 cells analysed.
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    brca2  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc brca2
    (A) . U2OS or Hela cells were transfected with Control (siCON), MCPH1 (siMCPH1) or <t>BRCA2</t> (siBRCA2) siRNA for 48h or 120h and protein expression analysed via Western blotting with the indicated antibodies. MCPH1 expression in sgCON (control) and sgMCPH1 (MCPH1 knockout) cells was also investigated by Western blotting. (B) Quantification of MCPH1 or BRCA2 protein bands normalised to GAPDH and expressed as % change relative to siCON or sgCON. Data is the mean and standard error of three (48h) or two (120h and sgCON/sgMCPH1) independent experiments. (C) U2OS or Hela cells were transfected with MCPH1 siRNA for 72h before analysis of nuclear morphology (premature chromosome condensation, PCC) via DAPI staining and fluorescence microscopy. (D) The % of cells displaying PCC, with at least 100 cells analysed.
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    Image Search Results


    The potential function and pathways of TSF on DOX‐induced cardiotoxicity: (a) PPI network of 103 overlapping targets constructed based on the STRING database; (b) the PPI network map; (c) the molecular docking of HDAC8 with BRCA2.

    Journal: Food Science & Nutrition

    Article Title: Exploring the Mechanisms of Total Saponins of Black Ginseng and Ginsenoside Rg3 Against Doxorubicin‐Induced Cardiotoxicity

    doi: 10.1002/fsn3.71968

    Figure Lengend Snippet: The potential function and pathways of TSF on DOX‐induced cardiotoxicity: (a) PPI network of 103 overlapping targets constructed based on the STRING database; (b) the PPI network map; (c) the molecular docking of HDAC8 with BRCA2.

    Article Snippet: Antibodies for BRCA2, DRP1 and Bcl‐2 were sourced from Wanlei Biotechnology Co. Ltd. (Lot: T11052438, T09113028, T11201556), while β ‐actin and HRP‐labeled secondary antibodies were procured from Affinity Biosciences (Lot: T0022, S0001).

    Techniques: Construct

    TSF and Rg3 regulate HDAC8/BRCA2/DRP1 pathway inhibits DOX‐induced cardiomyocyte apoptosis. (a) Apoptosis was assessed using TUNEL staining (Scale bar = 50 μm). (b) The results of western blotting for each group. (c) The expression of Bcl‐2 for each group. (d) The expression of DRP1 for each group. (e) The expression of HDAC8 for each group. (f) The expression of BRCA2 for each group. (g) Immunofluorescence staining for observing the colocalization of HDAC8, BRCA2, and DRP1 (Scale bar = 1 mm). Data are expressed as mean ± standard from three individual experiments. Data analysis among groups was performed using ANOVA, with LSD for equal variances and Dunnett’s‐T3 for unequal variances, with significance levels of * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Food Science & Nutrition

    Article Title: Exploring the Mechanisms of Total Saponins of Black Ginseng and Ginsenoside Rg3 Against Doxorubicin‐Induced Cardiotoxicity

    doi: 10.1002/fsn3.71968

    Figure Lengend Snippet: TSF and Rg3 regulate HDAC8/BRCA2/DRP1 pathway inhibits DOX‐induced cardiomyocyte apoptosis. (a) Apoptosis was assessed using TUNEL staining (Scale bar = 50 μm). (b) The results of western blotting for each group. (c) The expression of Bcl‐2 for each group. (d) The expression of DRP1 for each group. (e) The expression of HDAC8 for each group. (f) The expression of BRCA2 for each group. (g) Immunofluorescence staining for observing the colocalization of HDAC8, BRCA2, and DRP1 (Scale bar = 1 mm). Data are expressed as mean ± standard from three individual experiments. Data analysis among groups was performed using ANOVA, with LSD for equal variances and Dunnett’s‐T3 for unequal variances, with significance levels of * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Antibodies for BRCA2, DRP1 and Bcl‐2 were sourced from Wanlei Biotechnology Co. Ltd. (Lot: T11052438, T09113028, T11201556), while β ‐actin and HRP‐labeled secondary antibodies were procured from Affinity Biosciences (Lot: T0022, S0001).

    Techniques: TUNEL Assay, Staining, Western Blot, Expressing, Immunofluorescence

    The mechanism diagram of TSF and Rg3 alleviating DOX‐induced myocardial injury by regulating the HDAC8/BRCA2/DRP1 pathway.

    Journal: Food Science & Nutrition

    Article Title: Exploring the Mechanisms of Total Saponins of Black Ginseng and Ginsenoside Rg3 Against Doxorubicin‐Induced Cardiotoxicity

    doi: 10.1002/fsn3.71968

    Figure Lengend Snippet: The mechanism diagram of TSF and Rg3 alleviating DOX‐induced myocardial injury by regulating the HDAC8/BRCA2/DRP1 pathway.

    Article Snippet: Antibodies for BRCA2, DRP1 and Bcl‐2 were sourced from Wanlei Biotechnology Co. Ltd. (Lot: T11052438, T09113028, T11201556), while β ‐actin and HRP‐labeled secondary antibodies were procured from Affinity Biosciences (Lot: T0022, S0001).

    Techniques:

    (A) . U2OS or Hela cells were transfected with Control (siCON), MCPH1 (siMCPH1) or BRCA2 (siBRCA2) siRNA for 48h or 120h and protein expression analysed via Western blotting with the indicated antibodies. MCPH1 expression in sgCON (control) and sgMCPH1 (MCPH1 knockout) cells was also investigated by Western blotting. (B) Quantification of MCPH1 or BRCA2 protein bands normalised to GAPDH and expressed as % change relative to siCON or sgCON. Data is the mean and standard error of three (48h) or two (120h and sgCON/sgMCPH1) independent experiments. (C) U2OS or Hela cells were transfected with MCPH1 siRNA for 72h before analysis of nuclear morphology (premature chromosome condensation, PCC) via DAPI staining and fluorescence microscopy. (D) The % of cells displaying PCC, with at least 100 cells analysed.

    Journal: PLOS One

    Article Title: Differential sensitivity of MCPH1- and BRCA2-deficient cancer cells to PARP-1 inhibition

    doi: 10.1371/journal.pone.0345514

    Figure Lengend Snippet: (A) . U2OS or Hela cells were transfected with Control (siCON), MCPH1 (siMCPH1) or BRCA2 (siBRCA2) siRNA for 48h or 120h and protein expression analysed via Western blotting with the indicated antibodies. MCPH1 expression in sgCON (control) and sgMCPH1 (MCPH1 knockout) cells was also investigated by Western blotting. (B) Quantification of MCPH1 or BRCA2 protein bands normalised to GAPDH and expressed as % change relative to siCON or sgCON. Data is the mean and standard error of three (48h) or two (120h and sgCON/sgMCPH1) independent experiments. (C) U2OS or Hela cells were transfected with MCPH1 siRNA for 72h before analysis of nuclear morphology (premature chromosome condensation, PCC) via DAPI staining and fluorescence microscopy. (D) The % of cells displaying PCC, with at least 100 cells analysed.

    Article Snippet: Membranes were blocked with 5% dried skimmed milk/TBS-T (50mM Tris pH 7.6, 150mM NaCl and 0.2% v/v Tween-20) for a minimum of 1-hour prior to overnight incubation at 4°C with primary antibodies; MCPH1 (11962–1-AP, Proteintech), BRCA2 (29450–1-AP, Proteintech) and GAPDH (60004–1-Ig, Proteintech).

    Techniques: Transfection, Control, Expressing, Western Blot, Knock-Out, Staining, Fluorescence, Microscopy

    U2OS cells were transfected with control (siCON), BRCA2 (siBRCA2) or BRCA2 + MCPH1 (siBRCA2 + siMCPH1) siRNA for 48h before (A) Western blot analysis or (B) seeding into 96-well plates and treating with the indicated doses of AZD2461 or Talazoparib, with each treatment performed in triplicate. After 96-hours, cell viability was measured using an MTS assay. MTS data represents the mean and standard error from at least three independent experiments, with cell viability expressed as a % relative to the corresponding untreated sample. Statistical significance was measured using two-way ANOVA (p-values described in text, with significance set at p < 0.05).

    Journal: PLOS One

    Article Title: Differential sensitivity of MCPH1- and BRCA2-deficient cancer cells to PARP-1 inhibition

    doi: 10.1371/journal.pone.0345514

    Figure Lengend Snippet: U2OS cells were transfected with control (siCON), BRCA2 (siBRCA2) or BRCA2 + MCPH1 (siBRCA2 + siMCPH1) siRNA for 48h before (A) Western blot analysis or (B) seeding into 96-well plates and treating with the indicated doses of AZD2461 or Talazoparib, with each treatment performed in triplicate. After 96-hours, cell viability was measured using an MTS assay. MTS data represents the mean and standard error from at least three independent experiments, with cell viability expressed as a % relative to the corresponding untreated sample. Statistical significance was measured using two-way ANOVA (p-values described in text, with significance set at p < 0.05).

    Article Snippet: Membranes were blocked with 5% dried skimmed milk/TBS-T (50mM Tris pH 7.6, 150mM NaCl and 0.2% v/v Tween-20) for a minimum of 1-hour prior to overnight incubation at 4°C with primary antibodies; MCPH1 (11962–1-AP, Proteintech), BRCA2 (29450–1-AP, Proteintech) and GAPDH (60004–1-Ig, Proteintech).

    Techniques: Transfection, Control, Western Blot, MTS Assay