Journal: Cancer Research

Article Title: HPV-16 E7 Reveals a Link between DNA Replication Stress, Fanconi Anemia D2 Protein, and Alternative Lengthening of Telomere–Associated Promyelocytic Leukemia Bodies

doi: 10.1158/0008-5472.can-08-0224

Figure Lengend Snippet: Figure 3. FANCD2-positive APBs contain known APB components as well as BRCA2 and MUS81. Coimmunofluorescence microscopic analysis of U-2 OS cells stably expressing HPV-16 E7 for FANCD2 in combination with RPA32, BLM, RAD51, MRE11, BRCA2/FANCD1, or MUS81. MUS81-HA was transiently overexpressed in cells before processing for immunofluorescence staining for HA and FANCD2. Insets, APBs indicated by arrows. Nuclei stained with DAPI. Bar, 10 Am.

Article Snippet: Primary antibodies used for immunoblotting and immunofluorescence were 53BP1 (Novus Biologicals), actin (Sigma), ATM (Genetex), phosphorylated ATM at serine 1981 (Novus Biologicals), ATR (Genetex), BLM (Bethyl), BRCA2 (Calbiochem), 5¶-bromo-2¶ deoxyuridine (BrdUrd; Roche Diagnostics), FANCD2 (Genetex), HA (Santa Cruz Biotechnology), HPV-16 E7 (Santa Cruz Biotechnology), g-H2AX (Trevigen), MRE11 (Genetex), PML (Santa Cruz Biotechnology), RAD51 (Genetex), RPA32 (Lab Vision), and TRF2 (Imgenex).

Techniques: Stable Transfection, Expressing, Immunofluorescence, Staining

Journal: Frontiers in Oncology

Article Title: Prognostic value and experimental validation of atherosclerosis-derived pathogenic genes in colorectal cancer

doi: 10.3389/fonc.2025.1728087

Figure Lengend Snippet: Validation of the expression of 6 hub genes in CRC and AS by molecular biology experiments. (A-F) RT-qPCR validation of the differential expression of prognostic genes between normal and tumor tissues in CRC patients. (G) Western blot validation of the differential expression of 2 newly identified genes (HMMR and PALB2) between normal and tumor tissues in CRC. (H) Quantitative analysis of the relative grayscale values of Western blot bands for the 2 newly identified genes (HMMR and PALB2) in normal and tumor tissues of CRC. Data are presented as mean ± standard deviation (SD), with statistical significance indicated as * p < 0.05, *** p < 0.001, and **** p < 0.0001. ns indicates no statistical significance.

Article Snippet: The membrane was blocked with QuickBlockTM Blocking Buffer for Western Blot (Beyotime Biotechnology, Jiangsu, China) at room temperature for 1 h, washed with TBST, and incubated overnight at 4°C with primary antibodies: HMMR (1:2000, 15820-1-AP, Proteintech), PALB2 (1:2000, 14340-1-AP, Proteintech), and β-actin (1:10,000, 66009-1-Ig, Proteintech).

Techniques: Biomarker Discovery, Expressing, Quantitative RT-PCR, Quantitative Proteomics, Western Blot, Standard Deviation

Journal: Frontiers in Oncology

Article Title: Prognostic value and experimental validation of atherosclerosis-derived pathogenic genes in colorectal cancer

doi: 10.3389/fonc.2025.1728087

Figure Lengend Snippet: IHC staining validation. (A) The differential expression of PALB2 and HMMR in CRC tissues compared with normal tissues; (B) displays the differential expression of HMMR , PALB2 and PRR11 in AS tissues versus normal tissues. * p < 0.05.

Article Snippet: The membrane was blocked with QuickBlockTM Blocking Buffer for Western Blot (Beyotime Biotechnology, Jiangsu, China) at room temperature for 1 h, washed with TBST, and incubated overnight at 4°C with primary antibodies: HMMR (1:2000, 15820-1-AP, Proteintech), PALB2 (1:2000, 14340-1-AP, Proteintech), and β-actin (1:10,000, 66009-1-Ig, Proteintech).

Techniques: Immunohistochemistry, Biomarker Discovery, Quantitative Proteomics

Journal: Molecular cell

Article Title: Replication gaps are a key determinant of PARP inhibitor synthetic lethality with BRCA deficiency

doi: 10.1016/j.molcel.2021.06.011

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Antibodies for western blot analysis included anti-β-actin (Sigma), anti-FANCJ (E67), anti-BRCA1 (Cell Signaling Technology), anti-p21 (Cell Signaling Technology), anti-CHD4 (Abcam), anti-BRCA2 (Abcam), anti-53BP1 (Novus Biological), anti-RADX (CXorf57, Abcam), anti-RPA70/RPA1 (Cell Signaling Technology), anti-RPA32/RPA2 (Abcam), anti-FEN1 (Abcam), anti-PARP1 (Abcam), anti-XRCC1 (Abcam), anti-LIG1 (Santa Cruz), anti-LIG3 (GeneTex) and anti-H2B (Cell Signaling Technology), anti-Cleaved Caspase-3 (Cell Signaling Technology) and anti-PARP (Cleaved PARP1, Cell Signaling Technology).

Techniques: Recombinant, Electron Microscopy, Plasmid Preparation, Imaging, CCK-8 Assay, CRISPR, shRNA, Negative Control, Construct, Software, Transfection, DNA Extraction

Journal: Human Genetics and Genomics Advances

Article Title: Prediction of breast cancer risk based on flow variant analysis of circulating peripheral blood mononuclear cells

doi: 10.1016/j.xhgg.2022.100085

Figure Lengend Snippet: Replication of CR-B FVAs (A) Replication of BRCA1 nuclear localization, BRCA2 nuclear localization, and p53 in matched LCLs and PBMCs derived from the same individuals (N = 20). Correlation cofficients are shown. (B) Replication of BRCA1 nuclear localization, BRCA2 nuclear localization, and p53 phosphorylation of days 2–4 following sample collection, compared to day 1. Correlation cofficients are shown.

Article Snippet: Expression plasmids (1 μg) for BRCA1 (pDEST-FRT/T0-GFP-BRCA1, cat. no. 71116, GFP tag), BRCA2 (pMH-SFB-BRCA2, cat. no. 99395, SFP tag), PALB2 (pDEST-FRT/T0-GFP-PALB2, cat. no. 71113, GFP tag), and ATM (pcDNA3.1(+)FLAG-His-ATM WT, cat. no. 31985, Addgene, Watertown, MA) were transfected into WT LCLs or those with P/LP or B/LB variants.

Techniques: Derivative Assay, Phospho-proteomics

Journal: Human Genetics and Genomics Advances

Article Title: Prediction of breast cancer risk based on flow variant analysis of circulating peripheral blood mononuclear cells

doi: 10.1016/j.xhgg.2022.100085

Figure Lengend Snippet: Sensitivity, specificity, and accuracy of FVAs and RCS for Coriell, Montefiore, and Northwell cohorts and all cohorts

Article Snippet: Expression plasmids (1 μg) for BRCA1 (pDEST-FRT/T0-GFP-BRCA1, cat. no. 71116, GFP tag), BRCA2 (pMH-SFB-BRCA2, cat. no. 99395, SFP tag), PALB2 (pDEST-FRT/T0-GFP-PALB2, cat. no. 71113, GFP tag), and ATM (pcDNA3.1(+)FLAG-His-ATM WT, cat. no. 31985, Addgene, Watertown, MA) were transfected into WT LCLs or those with P/LP or B/LB variants.

Techniques:

Journal: Human Genetics and Genomics Advances

Article Title: Prediction of breast cancer risk based on flow variant analysis of circulating peripheral blood mononuclear cells

doi: 10.1016/j.xhgg.2022.100085

Figure Lengend Snippet: Expression plasmid rescue of genetic variants in LCLs (A–D) Gene rescue for (A) BRCA1 , (B) BRCA2 , (C) ATM , and (D) PALB2 variants by RCS by boxplots. P-values for pairwise comparisons are shown.

Article Snippet: Expression plasmids (1 μg) for BRCA1 (pDEST-FRT/T0-GFP-BRCA1, cat. no. 71116, GFP tag), BRCA2 (pMH-SFB-BRCA2, cat. no. 99395, SFP tag), PALB2 (pDEST-FRT/T0-GFP-PALB2, cat. no. 71113, GFP tag), and ATM (pcDNA3.1(+)FLAG-His-ATM WT, cat. no. 31985, Addgene, Watertown, MA) were transfected into WT LCLs or those with P/LP or B/LB variants.

Techniques: Expressing, Plasmid Preparation