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biotinylated gsa b4  (Vector Laboratories)


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    Structured Review

    Vector Laboratories biotinylated gsa b4
    Biotinylated Gsa B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated gsa b4/product/Vector Laboratories
    Average 95 stars, based on 1 article reviews
    biotinylated gsa b4 - by Bioz Stars, 2025-02
    95/100 stars

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    Clustered VEGF nanoparticles (CLUVENA) promote de novo formation of perfused vessels. A) Representative immunofluorescent 20x images stained for perfused vessels (Tomato <t>Lectin)</t> in white and nuclei (DAPI) in blue. B) Experimental timeline and image analysis schematic. Day 15 and 35 quantification of C) normalized perfused vessel area, D) perfused vessel infiltration, and vessel intensity as well as E) direct comparison between day 15 and 35. For panels C-E representing one timepoint, each data point is a biological replicate averaged from two coronal sections and plotted in a floating bar (min to max). For panels C-E comparing day 15 and 35, averaged values are plotted with error bars represent s.d.. n = 4 – 6. For panels C-E, one-way ANOVA with Tukey HSD post-hoc analysis was performed. To compare across time within treatment groups, two-way ANOVA with Sidak post-hoc analysis was performed. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Scale bars represent 200 μm.
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    Vector Laboratories biotinylated maackia amurensis lectin ii
    A) Schematic describing <t>lectin</t> flow cytometry and representative glycan epitopes. B, C, D) Sperm were exposed to sialidases (AUS 20 units or GvNanH2 160 units) for 1 hour, fixed in formalin, and stained with MAL-II-biotin/Neutravidin-Alexa Fluor 488, SNA-Cy5, or PSA-FITC respectively. Each point represents the median fluorescence intensity from one donor. Statistics represent a mixed model comparison with post-hoc Holm-Šidák tests. E) Schematic representing change in zeta potential after removal of negatively charged sialic acid on sperm surface. F) Sperm were treated with sialidase at various doses for 1 hour, and zeta potential was measured via dynamic light scattering. Each measurement represents the average of seven technical replicates, and statistics represent a mixed model comparison with post hoc Holm-Šidák tests.
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    A) Schematic describing <t>lectin</t> flow cytometry and representative glycan epitopes. B, C, D) Sperm were exposed to sialidases (AUS 20 units or GvNanH2 160 units) for 1 hour, fixed in formalin, and stained with MAL-II-biotin/Neutravidin-Alexa Fluor 488, SNA-Cy5, or PSA-FITC respectively. Each point represents the median fluorescence intensity from one donor. Statistics represent a mixed model comparison with post-hoc Holm-Šidák tests. E) Schematic representing change in zeta potential after removal of negatively charged sialic acid on sperm surface. F) Sperm were treated with sialidase at various doses for 1 hour, and zeta potential was measured via dynamic light scattering. Each measurement represents the average of seven technical replicates, and statistics represent a mixed model comparison with post hoc Holm-Šidák tests.
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    A) Schematic describing <t>lectin</t> flow cytometry and representative glycan epitopes. B, C, D) Sperm were exposed to sialidases (AUS 20 units or GvNanH2 160 units) for 1 hour, fixed in formalin, and stained with MAL-II-biotin/Neutravidin-Alexa Fluor 488, SNA-Cy5, or PSA-FITC respectively. Each point represents the median fluorescence intensity from one donor. Statistics represent a mixed model comparison with post-hoc Holm-Šidák tests. E) Schematic representing change in zeta potential after removal of negatively charged sialic acid on sperm surface. F) Sperm were treated with sialidase at various doses for 1 hour, and zeta potential was measured via dynamic light scattering. Each measurement represents the average of seven technical replicates, and statistics represent a mixed model comparison with post hoc Holm-Šidák tests.
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    A) Schematic describing <t>lectin</t> flow cytometry and representative glycan epitopes. B, C, D) Sperm were exposed to sialidases (AUS 20 units or GvNanH2 160 units) for 1 hour, fixed in formalin, and stained with MAL-II-biotin/Neutravidin-Alexa Fluor 488, SNA-Cy5, or PSA-FITC respectively. Each point represents the median fluorescence intensity from one donor. Statistics represent a mixed model comparison with post-hoc Holm-Šidák tests. E) Schematic representing change in zeta potential after removal of negatively charged sialic acid on sperm surface. F) Sperm were treated with sialidase at various doses for 1 hour, and zeta potential was measured via dynamic light scattering. Each measurement represents the average of seven technical replicates, and statistics represent a mixed model comparison with post hoc Holm-Šidák tests.
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    Image Search Results


    Clustered VEGF nanoparticles (CLUVENA) promote de novo formation of perfused vessels. A) Representative immunofluorescent 20x images stained for perfused vessels (Tomato Lectin) in white and nuclei (DAPI) in blue. B) Experimental timeline and image analysis schematic. Day 15 and 35 quantification of C) normalized perfused vessel area, D) perfused vessel infiltration, and vessel intensity as well as E) direct comparison between day 15 and 35. For panels C-E representing one timepoint, each data point is a biological replicate averaged from two coronal sections and plotted in a floating bar (min to max). For panels C-E comparing day 15 and 35, averaged values are plotted with error bars represent s.d.. n = 4 – 6. For panels C-E, one-way ANOVA with Tukey HSD post-hoc analysis was performed. To compare across time within treatment groups, two-way ANOVA with Sidak post-hoc analysis was performed. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Scale bars represent 200 μm.

    Journal: bioRxiv

    Article Title: Clustered VEGF Nanoparticles in Microporous Annealed Particle (MAP) Hydrogel Accelerates Functional Recovery and Brain Tissue Repair after Stroke

    doi: 10.1101/2025.01.30.635733

    Figure Lengend Snippet: Clustered VEGF nanoparticles (CLUVENA) promote de novo formation of perfused vessels. A) Representative immunofluorescent 20x images stained for perfused vessels (Tomato Lectin) in white and nuclei (DAPI) in blue. B) Experimental timeline and image analysis schematic. Day 15 and 35 quantification of C) normalized perfused vessel area, D) perfused vessel infiltration, and vessel intensity as well as E) direct comparison between day 15 and 35. For panels C-E representing one timepoint, each data point is a biological replicate averaged from two coronal sections and plotted in a floating bar (min to max). For panels C-E comparing day 15 and 35, averaged values are plotted with error bars represent s.d.. n = 4 – 6. For panels C-E, one-way ANOVA with Tukey HSD post-hoc analysis was performed. To compare across time within treatment groups, two-way ANOVA with Sidak post-hoc analysis was performed. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Scale bars represent 200 μm.

    Article Snippet: At terminal endpoints, mice were anesthetized with isoflurane, after which 50 μL of biotinylated Lycopersicon esculentum (tomato) Lectin (Vector Labs, Burlingame, CA) was administered via a retro-orbital injection.

    Techniques: Staining, Comparison

    Astrocytic end-feet overlap de novo vessels within MAP. A) Representative immunofluorescent 20x images stained for astrocytic end-feet (AQP4) in purple, perfused vessels (Tomato Lectin) in yellow and nuclei (DAPI) in cyan. B) Experimental timeline and image analysis schematic. C) AQP4 coverage on TL in infarct on days 15 and 35, and a direct comparison of AQP4 coverage on TL between days 15 and 35. D) Immunofluorescent 60x images stained for astrocytic end-feet (AQP4) in purple, perfused vessels (Tomato Lectin) in yellow and nuclei (DAPI) in cyan. For panels C representing one timepoint, each data point is a biological replicate averaged from two coronal sections and plotted in a floating bar (min to max). For panels C comparing day 15 and 35, averaged values are plotted with error bars represent s.d. n = 4 – 5. For panels B-C, one-way ANOVA with a Tukey HSD post-hoc analysis was performed. To compare across time within treatment groups, two-way ANOVA with Sidak post-hoc analysis was performed. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Scale bars in panel A represent 200 μm and in panel D represent 50 μm.

    Journal: bioRxiv

    Article Title: Clustered VEGF Nanoparticles in Microporous Annealed Particle (MAP) Hydrogel Accelerates Functional Recovery and Brain Tissue Repair after Stroke

    doi: 10.1101/2025.01.30.635733

    Figure Lengend Snippet: Astrocytic end-feet overlap de novo vessels within MAP. A) Representative immunofluorescent 20x images stained for astrocytic end-feet (AQP4) in purple, perfused vessels (Tomato Lectin) in yellow and nuclei (DAPI) in cyan. B) Experimental timeline and image analysis schematic. C) AQP4 coverage on TL in infarct on days 15 and 35, and a direct comparison of AQP4 coverage on TL between days 15 and 35. D) Immunofluorescent 60x images stained for astrocytic end-feet (AQP4) in purple, perfused vessels (Tomato Lectin) in yellow and nuclei (DAPI) in cyan. For panels C representing one timepoint, each data point is a biological replicate averaged from two coronal sections and plotted in a floating bar (min to max). For panels C comparing day 15 and 35, averaged values are plotted with error bars represent s.d. n = 4 – 5. For panels B-C, one-way ANOVA with a Tukey HSD post-hoc analysis was performed. To compare across time within treatment groups, two-way ANOVA with Sidak post-hoc analysis was performed. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Scale bars in panel A represent 200 μm and in panel D represent 50 μm.

    Article Snippet: At terminal endpoints, mice were anesthetized with isoflurane, after which 50 μL of biotinylated Lycopersicon esculentum (tomato) Lectin (Vector Labs, Burlingame, CA) was administered via a retro-orbital injection.

    Techniques: Staining, Comparison

    A) Schematic describing lectin flow cytometry and representative glycan epitopes. B, C, D) Sperm were exposed to sialidases (AUS 20 units or GvNanH2 160 units) for 1 hour, fixed in formalin, and stained with MAL-II-biotin/Neutravidin-Alexa Fluor 488, SNA-Cy5, or PSA-FITC respectively. Each point represents the median fluorescence intensity from one donor. Statistics represent a mixed model comparison with post-hoc Holm-Šidák tests. E) Schematic representing change in zeta potential after removal of negatively charged sialic acid on sperm surface. F) Sperm were treated with sialidase at various doses for 1 hour, and zeta potential was measured via dynamic light scattering. Each measurement represents the average of seven technical replicates, and statistics represent a mixed model comparison with post hoc Holm-Šidák tests.

    Journal: bioRxiv

    Article Title: Sialidases derived from Gardnerella vaginalis remodel the sperm glycocalyx and impair sperm function

    doi: 10.1101/2025.02.01.636076

    Figure Lengend Snippet: A) Schematic describing lectin flow cytometry and representative glycan epitopes. B, C, D) Sperm were exposed to sialidases (AUS 20 units or GvNanH2 160 units) for 1 hour, fixed in formalin, and stained with MAL-II-biotin/Neutravidin-Alexa Fluor 488, SNA-Cy5, or PSA-FITC respectively. Each point represents the median fluorescence intensity from one donor. Statistics represent a mixed model comparison with post-hoc Holm-Šidák tests. E) Schematic representing change in zeta potential after removal of negatively charged sialic acid on sperm surface. F) Sperm were treated with sialidase at various doses for 1 hour, and zeta potential was measured via dynamic light scattering. Each measurement represents the average of seven technical replicates, and statistics represent a mixed model comparison with post hoc Holm-Šidák tests.

    Article Snippet: Biotinylated Maackia Amurensis Lectin II (MAL II, Cat #:B-1265-1) and Cy5-conjugated Sambucus Nigra Lectin (SNA, Cat #: CL-1305-1) were purchased from Vector Labs. Human Contraception Antibody (HCA), was a gift from ZabBio.

    Techniques: Flow Cytometry, Staining, Fluorescence, Comparison, Zeta Potential Analyzer