Review




Structured Review

Proteintech atf4
Protein expression analysis of ER stress and autophagy markers by Western blotting. (A) Representative Western blot images showing the protein levels of Bip, p62, <t>ATF4,</t> Beclin-1, Atg5, β-actin, eIF2α, p-eIF2α, CHOP, LC3I and LC3II. (B–I) Quantification of relative protein expression. Protein levels were normalized to β-actin, except for the LC3II/LC3I ratio, a ratio between two specific protein bands. Data are presented as mean ± SD. * P < 0.05 and ** P < 0.01 compared to the model group. n = 3 Per group.
Atf4, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 635 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/atf4/ATF4+Antibody/pmc13638362-110-25-28
Average 96 stars, based on 635 article reviews
atf4 - by Bioz Stars, 2026-10
96/100 stars

Images

1) Product Images from "Codonopsis pilosula inulin-type fructan CPA ameliorates diarrhea-predominant irritable bowel syndrome via regulation of ER stress-induced autophagy and modulation of gut microbiota"

Article Title: Codonopsis pilosula inulin-type fructan CPA ameliorates diarrhea-predominant irritable bowel syndrome via regulation of ER stress-induced autophagy and modulation of gut microbiota

Journal: Frontiers in Pharmacology

doi: 10.3389/fphar.2026.1715972

Protein expression analysis of ER stress and autophagy markers by Western blotting. (A) Representative Western blot images showing the protein levels of Bip, p62, ATF4, Beclin-1, Atg5, β-actin, eIF2α, p-eIF2α, CHOP, LC3I and LC3II. (B–I) Quantification of relative protein expression. Protein levels were normalized to β-actin, except for the LC3II/LC3I ratio, a ratio between two specific protein bands. Data are presented as mean ± SD. * P < 0.05 and ** P < 0.01 compared to the model group. n = 3 Per group.
Figure Legend Snippet: Protein expression analysis of ER stress and autophagy markers by Western blotting. (A) Representative Western blot images showing the protein levels of Bip, p62, ATF4, Beclin-1, Atg5, β-actin, eIF2α, p-eIF2α, CHOP, LC3I and LC3II. (B–I) Quantification of relative protein expression. Protein levels were normalized to β-actin, except for the LC3II/LC3I ratio, a ratio between two specific protein bands. Data are presented as mean ± SD. * P < 0.05 and ** P < 0.01 compared to the model group. n = 3 Per group.

Techniques Used: Expressing, Analysis, Western Blot

Protein expression analysis of ER stress markers by Western blotting in NCM460 cells. (A) Representative Western blot images showing the protein levels of Bip, ATF4, β-actin, eIF2α, p-eIF2α, and CHOP in NCM460 cells from different treatment groups. (B–E) Quantification of relative protein expression. Protein levels were normalized to β-actin, except for the p-eIF2α/eIF2α ratio. Data are presented as mean ± SD. * P < 0.05 and ** P < 0.01 compared to the LPS group. n = 3 Per group.
Figure Legend Snippet: Protein expression analysis of ER stress markers by Western blotting in NCM460 cells. (A) Representative Western blot images showing the protein levels of Bip, ATF4, β-actin, eIF2α, p-eIF2α, and CHOP in NCM460 cells from different treatment groups. (B–E) Quantification of relative protein expression. Protein levels were normalized to β-actin, except for the p-eIF2α/eIF2α ratio. Data are presented as mean ± SD. * P < 0.05 and ** P < 0.01 compared to the LPS group. n = 3 Per group.

Techniques Used: Expressing, Analysis, Western Blot

Related Articles

Immunofluorescence:

Article Title: Natural photosynthetic system for restoring homeostasis of animal organelle interaction network
Article Snippet: The following antibodies were used for western blotting: CHOP (Proteintech, 15204-1-AP, 1:1,500); IRE1 (Proteintech, 27528-1-AP, 1:1,500); ATF6 (Proteintech, 66563-1-Ig, 1:10,000); ATF4 (Proteintech, 60035-1-Ig, 1:2000);β-actin (Proteintech, 20536-1-AP, 1:5000). .. For immunofluorescence analysis, the following antibodies were used: KRT19 (Proteintech, 10712-1-AP, 1:200), CD24 (Proteintech, 10600-1-AP, 1:200), CHOP (Proteintech, 15204-1-AP, 1:100); ATF4 (Proteintech, 60035-1-Ig, 1:200); Col II (Proteintech, 28459-1-AP, 1:800); aggrecan (Proteintech, 13880-1-AP, 1:200); MMP13 (Proteintech, 18165-1-AP, 1:200); ADAMTS-5 (Affinity Biosciences, DF13268, 1:200); TOMM20 (Abcam, ab56783, 1:200); Calnexin (Proteintech, 10427-2-AP, 1:200). .. Human NPC viability was evaluated using two independent assays: the MTT assay (Beyotime) and the live/dead staining assay (Beyotime).

Incubation:

Article Title: Capsaicin attenuates methylglyoxal-induced neurotoxicity via the ATF4-Sestrin2 signaling and direct scavenging of methylglyoxal.
Article Snippet: Methylglyoxal (MGO) is a highly reactive dicarbonyl byproduct of glycolysis.. It accumulates under pathological conditions and exhibits pronounced cytotoxic effects in neuronal cells.. Capsaicin, a dietary vanilloid compound and agonist of the transient receptor potential vanilloid 1 (TRPV1) channel, has been reported to exert cytoprotective effects; however, its molecular mechanisms in carbonyl stress remain poorly defined.

Article Title: Prevotella histicola improves metabolic-associated fatty liver disease by promoting lipid droplet autophagy and inhibiting ER stress.
Article Snippet: Metabolically associated fatty liver disease (MAFLD), a prevalent chronic liver disorder linked to metabolic dysfunction, is marked by hepatic lipid accumulation and inflammatory response.. Treatment options are limited, yet probiotics show potential in modulating the gut-liver axis.. Prevotella histicola (P. histicola), known for its anti-oxidant and anti-inflammatory features, provides protection against metabolic diseases, but its role in MAFLD remains unclear.

Western Blot:

Article Title: Natural photosynthetic system for restoring homeostasis of animal organelle interaction network
Article Snippet: SDS-PAGE and Coomassie Blue (Beyotime) staining were used to detect proteins in the thylakoids, NTUs, NPC membranes, and NM-NTUs. .. The following antibodies were used for western blotting: CHOP (Proteintech, 15204-1-AP, 1:1,500); IRE1 (Proteintech, 27528-1-AP, 1:1,500); ATF6 (Proteintech, 66563-1-Ig, 1:10,000); ATF4 (Proteintech, 60035-1-Ig, 1:2000);β-actin (Proteintech, 20536-1-AP, 1:5000). .. For immunofluorescence analysis, the following antibodies were used: KRT19 (Proteintech, 10712-1-AP, 1:200), CD24 (Proteintech, 10600-1-AP, 1:200), CHOP (Proteintech, 15204-1-AP, 1:100); ATF4 (Proteintech, 60035-1-Ig, 1:200); Col II (Proteintech, 28459-1-AP, 1:800); aggrecan (Proteintech, 13880-1-AP, 1:200); MMP13 (Proteintech, 18165-1-AP, 1:200); ADAMTS-5 (Affinity Biosciences, DF13268, 1:200); TOMM20 (Abcam, ab56783, 1:200); Calnexin (Proteintech, 10427-2-AP, 1:200).

Membrane:

Article Title: Prevotella histicola improves metabolic-associated fatty liver disease by promoting lipid droplet autophagy and inhibiting ER stress.
Article Snippet: Metabolically associated fatty liver disease (MAFLD), a prevalent chronic liver disorder linked to metabolic dysfunction, is marked by hepatic lipid accumulation and inflammatory response.. Treatment options are limited, yet probiotics show potential in modulating the gut-liver axis.. Prevotella histicola (P. histicola), known for its anti-oxidant and anti-inflammatory features, provides protection against metabolic diseases, but its role in MAFLD remains unclear.

Blocking Assay:

Article Title: Prevotella histicola improves metabolic-associated fatty liver disease by promoting lipid droplet autophagy and inhibiting ER stress.
Article Snippet: Metabolically associated fatty liver disease (MAFLD), a prevalent chronic liver disorder linked to metabolic dysfunction, is marked by hepatic lipid accumulation and inflammatory response.. Treatment options are limited, yet probiotics show potential in modulating the gut-liver axis.. Prevotella histicola (P. histicola), known for its anti-oxidant and anti-inflammatory features, provides protection against metabolic diseases, but its role in MAFLD remains unclear.



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Protein expression analysis of ER stress and autophagy markers by Western blotting. (A) Representative Western blot images showing the protein levels of Bip, p62, <t>ATF4,</t> Beclin-1, Atg5, β-actin, eIF2α, p-eIF2α, CHOP, LC3I and LC3II. (B–I) Quantification of relative protein expression. Protein levels were normalized to β-actin, except for the LC3II/LC3I ratio, a ratio between two specific protein bands. Data are presented as mean ± SD. * P < 0.05 and ** P < 0.01 compared to the model group. n = 3 Per group.
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Protein expression analysis of ER stress and autophagy markers by Western blotting. (A) Representative Western blot images showing the protein levels of Bip, p62, <t>ATF4,</t> Beclin-1, Atg5, β-actin, eIF2α, p-eIF2α, CHOP, LC3I and LC3II. (B–I) Quantification of relative protein expression. Protein levels were normalized to β-actin, except for the LC3II/LC3I ratio, a ratio between two specific protein bands. Data are presented as mean ± SD. * P < 0.05 and ** P < 0.01 compared to the model group. n = 3 Per group.
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Protein expression analysis of ER stress and autophagy markers by Western blotting. (A) Representative Western blot images showing the protein levels of Bip, p62, <t>ATF4,</t> Beclin-1, Atg5, β-actin, eIF2α, p-eIF2α, CHOP, LC3I and LC3II. (B–I) Quantification of relative protein expression. Protein levels were normalized to β-actin, except for the LC3II/LC3I ratio, a ratio between two specific protein bands. Data are presented as mean ± SD. * P < 0.05 and ** P < 0.01 compared to the model group. n = 3 Per group.
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Protein expression analysis of ER stress and autophagy markers by Western blotting. (A) Representative Western blot images showing the protein levels of Bip, p62, <t>ATF4,</t> Beclin-1, Atg5, β-actin, eIF2α, p-eIF2α, CHOP, LC3I and LC3II. (B–I) Quantification of relative protein expression. Protein levels were normalized to β-actin, except for the LC3II/LC3I ratio, a ratio between two specific protein bands. Data are presented as mean ± SD. * P < 0.05 and ** P < 0.01 compared to the model group. n = 3 Per group.
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Protein expression analysis of ER stress and autophagy markers by Western blotting. (A) Representative Western blot images showing the protein levels of Bip, p62, <t>ATF4,</t> Beclin-1, Atg5, β-actin, eIF2α, p-eIF2α, CHOP, LC3I and LC3II. (B–I) Quantification of relative protein expression. Protein levels were normalized to β-actin, except for the LC3II/LC3I ratio, a ratio between two specific protein bands. Data are presented as mean ± SD. * P < 0.05 and ** P < 0.01 compared to the model group. n = 3 Per group.
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Image Search Results


Protein expression analysis of ER stress and autophagy markers by Western blotting. (A) Representative Western blot images showing the protein levels of Bip, p62, ATF4, Beclin-1, Atg5, β-actin, eIF2α, p-eIF2α, CHOP, LC3I and LC3II. (B–I) Quantification of relative protein expression. Protein levels were normalized to β-actin, except for the LC3II/LC3I ratio, a ratio between two specific protein bands. Data are presented as mean ± SD. * P < 0.05 and ** P < 0.01 compared to the model group. n = 3 Per group.

Journal: Frontiers in Pharmacology

Article Title: Codonopsis pilosula inulin-type fructan CPA ameliorates diarrhea-predominant irritable bowel syndrome via regulation of ER stress-induced autophagy and modulation of gut microbiota

doi: 10.3389/fphar.2026.1715972

Figure Lengend Snippet: Protein expression analysis of ER stress and autophagy markers by Western blotting. (A) Representative Western blot images showing the protein levels of Bip, p62, ATF4, Beclin-1, Atg5, β-actin, eIF2α, p-eIF2α, CHOP, LC3I and LC3II. (B–I) Quantification of relative protein expression. Protein levels were normalized to β-actin, except for the LC3II/LC3I ratio, a ratio between two specific protein bands. Data are presented as mean ± SD. * P < 0.05 and ** P < 0.01 compared to the model group. n = 3 Per group.

Article Snippet: The primary and secondary antibodies, along with their dilutions, were listed as follows: P-eIF2α (1:5,000, 28740-1-AP, Proteintech), eIF2α (1:1,000, 11170-1-AP, Proteintech), eIF2α (1:500, A0764, ABclonal), ATF4 (1:750, 10835-1-AP, Proteintech), CHOP (1:1,500, 15204-1-AP), GRP78/BIP (1:6,000, 11587-1-AP), ATG5 (1:4,500, 10181-2-AP), Beclin-1 (1:5,000, 11306-1-AP, Proteintech), P62 (1:1,000, A19700, ABclonal), LC3B (YN5524, 1:1,500, Immunoway), β-actin (1:7,000, 20536-1-AP, Proteintech).

Techniques: Expressing, Analysis, Western Blot

Protein expression analysis of ER stress markers by Western blotting in NCM460 cells. (A) Representative Western blot images showing the protein levels of Bip, ATF4, β-actin, eIF2α, p-eIF2α, and CHOP in NCM460 cells from different treatment groups. (B–E) Quantification of relative protein expression. Protein levels were normalized to β-actin, except for the p-eIF2α/eIF2α ratio. Data are presented as mean ± SD. * P < 0.05 and ** P < 0.01 compared to the LPS group. n = 3 Per group.

Journal: Frontiers in Pharmacology

Article Title: Codonopsis pilosula inulin-type fructan CPA ameliorates diarrhea-predominant irritable bowel syndrome via regulation of ER stress-induced autophagy and modulation of gut microbiota

doi: 10.3389/fphar.2026.1715972

Figure Lengend Snippet: Protein expression analysis of ER stress markers by Western blotting in NCM460 cells. (A) Representative Western blot images showing the protein levels of Bip, ATF4, β-actin, eIF2α, p-eIF2α, and CHOP in NCM460 cells from different treatment groups. (B–E) Quantification of relative protein expression. Protein levels were normalized to β-actin, except for the p-eIF2α/eIF2α ratio. Data are presented as mean ± SD. * P < 0.05 and ** P < 0.01 compared to the LPS group. n = 3 Per group.

Article Snippet: The primary and secondary antibodies, along with their dilutions, were listed as follows: P-eIF2α (1:5,000, 28740-1-AP, Proteintech), eIF2α (1:1,000, 11170-1-AP, Proteintech), eIF2α (1:500, A0764, ABclonal), ATF4 (1:750, 10835-1-AP, Proteintech), CHOP (1:1,500, 15204-1-AP), GRP78/BIP (1:6,000, 11587-1-AP), ATG5 (1:4,500, 10181-2-AP), Beclin-1 (1:5,000, 11306-1-AP, Proteintech), P62 (1:1,000, A19700, ABclonal), LC3B (YN5524, 1:1,500, Immunoway), β-actin (1:7,000, 20536-1-AP, Proteintech).

Techniques: Expressing, Analysis, Western Blot