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Protein expression analysis of ER stress and autophagy markers by Western blotting. (A) Representative Western blot images showing the protein levels of Bip, p62, <t>ATF4,</t> Beclin-1, Atg5, β-actin, eIF2α, p-eIF2α, CHOP, LC3I and LC3II. (B–I) Quantification of relative protein expression. Protein levels were normalized to β-actin, except for the LC3II/LC3I ratio, a ratio between two specific protein bands. Data are presented as mean ± SD. * P < 0.05 and ** P < 0.01 compared to the model group. n = 3 Per group.
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Protein expression analysis of ER stress and autophagy markers by Western blotting. (A) Representative Western blot images showing the protein levels of Bip, p62, <t>ATF4,</t> Beclin-1, Atg5, β-actin, eIF2α, p-eIF2α, CHOP, LC3I and LC3II. (B–I) Quantification of relative protein expression. Protein levels were normalized to β-actin, except for the LC3II/LC3I ratio, a ratio between two specific protein bands. Data are presented as mean ± SD. * P < 0.05 and ** P < 0.01 compared to the model group. n = 3 Per group.
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Protein expression analysis of ER stress and autophagy markers by Western blotting. (A) Representative Western blot images showing the protein levels of Bip, p62, <t>ATF4,</t> Beclin-1, Atg5, β-actin, eIF2α, p-eIF2α, CHOP, LC3I and LC3II. (B–I) Quantification of relative protein expression. Protein levels were normalized to β-actin, except for the LC3II/LC3I ratio, a ratio between two specific protein bands. Data are presented as mean ± SD. * P < 0.05 and ** P < 0.01 compared to the model group. n = 3 Per group.
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Protein expression analysis of ER stress and autophagy markers by Western blotting. (A) Representative Western blot images showing the protein levels of Bip, p62, <t>ATF4,</t> Beclin-1, Atg5, β-actin, eIF2α, p-eIF2α, CHOP, LC3I and LC3II. (B–I) Quantification of relative protein expression. Protein levels were normalized to β-actin, except for the LC3II/LC3I ratio, a ratio between two specific protein bands. Data are presented as mean ± SD. * P < 0.05 and ** P < 0.01 compared to the model group. n = 3 Per group.
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Image Search Results


Protein expression analysis of ER stress and autophagy markers by Western blotting. (A) Representative Western blot images showing the protein levels of Bip, p62, ATF4, Beclin-1, Atg5, β-actin, eIF2α, p-eIF2α, CHOP, LC3I and LC3II. (B–I) Quantification of relative protein expression. Protein levels were normalized to β-actin, except for the LC3II/LC3I ratio, a ratio between two specific protein bands. Data are presented as mean ± SD. * P < 0.05 and ** P < 0.01 compared to the model group. n = 3 Per group.

Journal: Frontiers in Pharmacology

Article Title: Codonopsis pilosula inulin-type fructan CPA ameliorates diarrhea-predominant irritable bowel syndrome via regulation of ER stress-induced autophagy and modulation of gut microbiota

doi: 10.3389/fphar.2026.1715972

Figure Lengend Snippet: Protein expression analysis of ER stress and autophagy markers by Western blotting. (A) Representative Western blot images showing the protein levels of Bip, p62, ATF4, Beclin-1, Atg5, β-actin, eIF2α, p-eIF2α, CHOP, LC3I and LC3II. (B–I) Quantification of relative protein expression. Protein levels were normalized to β-actin, except for the LC3II/LC3I ratio, a ratio between two specific protein bands. Data are presented as mean ± SD. * P < 0.05 and ** P < 0.01 compared to the model group. n = 3 Per group.

Article Snippet: The primary and secondary antibodies, along with their dilutions, were listed as follows: P-eIF2α (1:5,000, 28740-1-AP, Proteintech), eIF2α (1:1,000, 11170-1-AP, Proteintech), eIF2α (1:500, A0764, ABclonal), ATF4 (1:750, 10835-1-AP, Proteintech), CHOP (1:1,500, 15204-1-AP), GRP78/BIP (1:6,000, 11587-1-AP), ATG5 (1:4,500, 10181-2-AP), Beclin-1 (1:5,000, 11306-1-AP, Proteintech), P62 (1:1,000, A19700, ABclonal), LC3B (YN5524, 1:1,500, Immunoway), β-actin (1:7,000, 20536-1-AP, Proteintech).

Techniques: Expressing, Analysis, Western Blot

Protein expression analysis of ER stress markers by Western blotting in NCM460 cells. (A) Representative Western blot images showing the protein levels of Bip, ATF4, β-actin, eIF2α, p-eIF2α, and CHOP in NCM460 cells from different treatment groups. (B–E) Quantification of relative protein expression. Protein levels were normalized to β-actin, except for the p-eIF2α/eIF2α ratio. Data are presented as mean ± SD. * P < 0.05 and ** P < 0.01 compared to the LPS group. n = 3 Per group.

Journal: Frontiers in Pharmacology

Article Title: Codonopsis pilosula inulin-type fructan CPA ameliorates diarrhea-predominant irritable bowel syndrome via regulation of ER stress-induced autophagy and modulation of gut microbiota

doi: 10.3389/fphar.2026.1715972

Figure Lengend Snippet: Protein expression analysis of ER stress markers by Western blotting in NCM460 cells. (A) Representative Western blot images showing the protein levels of Bip, ATF4, β-actin, eIF2α, p-eIF2α, and CHOP in NCM460 cells from different treatment groups. (B–E) Quantification of relative protein expression. Protein levels were normalized to β-actin, except for the p-eIF2α/eIF2α ratio. Data are presented as mean ± SD. * P < 0.05 and ** P < 0.01 compared to the LPS group. n = 3 Per group.

Article Snippet: The primary and secondary antibodies, along with their dilutions, were listed as follows: P-eIF2α (1:5,000, 28740-1-AP, Proteintech), eIF2α (1:1,000, 11170-1-AP, Proteintech), eIF2α (1:500, A0764, ABclonal), ATF4 (1:750, 10835-1-AP, Proteintech), CHOP (1:1,500, 15204-1-AP), GRP78/BIP (1:6,000, 11587-1-AP), ATG5 (1:4,500, 10181-2-AP), Beclin-1 (1:5,000, 11306-1-AP, Proteintech), P62 (1:1,000, A19700, ABclonal), LC3B (YN5524, 1:1,500, Immunoway), β-actin (1:7,000, 20536-1-AP, Proteintech).

Techniques: Expressing, Analysis, Western Blot