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anti atf4  (MedChemExpress)


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    Structured Review

    MedChemExpress anti atf4
    Anti Atf4, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atf4/ATF4+Antibody/pmc13443488-92-12-13
    Average 94 stars, based on 1 article reviews
    anti atf4 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Membrane:

    Article Title: Marek's disease virus hijacks host nucleotide metabolism via UL23-mediated c-Myc activation.
    Article Snippet: .. The membrane was then blocked in a solution containing 5% skimmed milk for 1 h. The membrane was incubated overnight at 4◦C with specific primary antibodies against mTOR (66888-1-Ig, Proteintech), ATF4 (HY-P80553, MedChemExpress), c-Myc (10828-1-AP, Proteintech), GMPS (A6606, Abclonal), gB, GAPDH (200306-7E4, zenbio), HISTONE H3 (BE3006, EASYBIO), β-actin (U33-386A, YOBlBlO), and Flag (20201, Promab, ProMab Biotechnologies Inc). .. After three washes with TBST, membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies(Absin) for 1 h at room temperature.

    Article Title: Oscillatory shear stress mediates endothelial cell senescence by regulating endoplasmic reticulum stress through HMOX1.
    Article Snippet: Oscillatory shear stress (OSS) can induce senescence in endothelial cells (ECs), driving atherosclerosis (AS), while the role of endoplasmic reticulum stress (ERS) remains unclear.. OSS induced senescence in ECs by increasing SAβ-gal staining levels and upregulating p53, p21, and p16 levels, as well as the senescence-associated secretory phenotype (SASP).. The SASP factors IL-1β, MIP-1α, and TNFα indicated the activation of the p53/p21 pathway.

    Incubation:

    Article Title: Marek's disease virus hijacks host nucleotide metabolism via UL23-mediated c-Myc activation.
    Article Snippet: .. The membrane was then blocked in a solution containing 5% skimmed milk for 1 h. The membrane was incubated overnight at 4◦C with specific primary antibodies against mTOR (66888-1-Ig, Proteintech), ATF4 (HY-P80553, MedChemExpress), c-Myc (10828-1-AP, Proteintech), GMPS (A6606, Abclonal), gB, GAPDH (200306-7E4, zenbio), HISTONE H3 (BE3006, EASYBIO), β-actin (U33-386A, YOBlBlO), and Flag (20201, Promab, ProMab Biotechnologies Inc). .. After three washes with TBST, membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies(Absin) for 1 h at room temperature.

    Article Title: Oscillatory shear stress mediates endothelial cell senescence by regulating endoplasmic reticulum stress through HMOX1.
    Article Snippet: Oscillatory shear stress (OSS) can induce senescence in endothelial cells (ECs), driving atherosclerosis (AS), while the role of endoplasmic reticulum stress (ERS) remains unclear.. OSS induced senescence in ECs by increasing SAβ-gal staining levels and upregulating p53, p21, and p16 levels, as well as the senescence-associated secretory phenotype (SASP).. The SASP factors IL-1β, MIP-1α, and TNFα indicated the activation of the p53/p21 pathway.

    Blocking Assay:

    Article Title: Oscillatory shear stress mediates endothelial cell senescence by regulating endoplasmic reticulum stress through HMOX1.
    Article Snippet: Oscillatory shear stress (OSS) can induce senescence in endothelial cells (ECs), driving atherosclerosis (AS), while the role of endoplasmic reticulum stress (ERS) remains unclear.. OSS induced senescence in ECs by increasing SAβ-gal staining levels and upregulating p53, p21, and p16 levels, as well as the senescence-associated secretory phenotype (SASP).. The SASP factors IL-1β, MIP-1α, and TNFα indicated the activation of the p53/p21 pathway.

    AST Assay:

    Article Title: Involvement of TMAO in exacerbating arsenic-induced neurotoxicity by activating the PERK/eIF2α/ATF4 pathway.
    Article Snippet: Observe the DG, CA1, and CA3 regions of the hippocampus for pathological changes using light microscopy, and acquire images (Zeiss). .. After fixing hippocampus coronal slices of mice and AST cells in 4 % paraformaldehyde (containing 0.5 % Triton X-100 for 30 min) and closed by goat serum (Beyotime, China), immunofluorescence staining was performed using primary antibodies against H2AX (1:200, Abcam, USA) and ATF4 (1:200, MCE, USA). ..

    Immunofluorescence:

    Article Title: Involvement of TMAO in exacerbating arsenic-induced neurotoxicity by activating the PERK/eIF2α/ATF4 pathway.
    Article Snippet: Observe the DG, CA1, and CA3 regions of the hippocampus for pathological changes using light microscopy, and acquire images (Zeiss). .. After fixing hippocampus coronal slices of mice and AST cells in 4 % paraformaldehyde (containing 0.5 % Triton X-100 for 30 min) and closed by goat serum (Beyotime, China), immunofluorescence staining was performed using primary antibodies against H2AX (1:200, Abcam, USA) and ATF4 (1:200, MCE, USA). ..

    Staining:

    Article Title: Involvement of TMAO in exacerbating arsenic-induced neurotoxicity by activating the PERK/eIF2α/ATF4 pathway.
    Article Snippet: Observe the DG, CA1, and CA3 regions of the hippocampus for pathological changes using light microscopy, and acquire images (Zeiss). .. After fixing hippocampus coronal slices of mice and AST cells in 4 % paraformaldehyde (containing 0.5 % Triton X-100 for 30 min) and closed by goat serum (Beyotime, China), immunofluorescence staining was performed using primary antibodies against H2AX (1:200, Abcam, USA) and ATF4 (1:200, MCE, USA). ..

    other:

    Article Title: Perfluorooctane sulfonate mediates GSH degradation leading to oral keratinocytes ferroptosis and mucositis through activation of the ER stress-ATF4-CHAC1 axis.
    Article Snippet: ATF4-IN-1 (HY-158201) and 4-PBA (HY-A0281) were procured from Med Chem Express (MCE, New Jersey, USA).



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