mgas6180  (ATCC)

Journal: Human Vaccines & Immunotherapeutics

Article Title: Population density profiles of nasopharyngeal carriage of 5 bacterial species in pre-school children measured using quantitative PCR offer potential insights into the dynamics of transmission

doi: 10.1080/21645515.2015.1090069

Figure Lengend Snippet: Target genes and primer and probe sequences used for qPCR assays. BHQ: Black Hole Quencher. *:Primer design from this study

Article Snippet: Streptococcus pyogenes , ATCC BAA-1064.

Techniques: Sequencing

Journal: Human Vaccines & Immunotherapeutics

Article Title: Population density profiles of nasopharyngeal carriage of 5 bacterial species in pre-school children measured using quantitative PCR offer potential insights into the dynamics of transmission

doi: 10.1080/21645515.2015.1090069

Figure Lengend Snippet: Bacterial strains used to prepare a panel of specificity controls

Article Snippet: Streptococcus pyogenes , ATCC BAA-1064.

Techniques:

Journal: Human Vaccines & Immunotherapeutics

Article Title: Population density profiles of nasopharyngeal carriage of 5 bacterial species in pre-school children measured using quantitative PCR offer potential insights into the dynamics of transmission

doi: 10.1080/21645515.2015.1090069

Figure Lengend Snippet: Bacterial strains used to prepare a panel of specificity controls

Article Snippet: Streptococcus pyogenes , ATCC BAA-1064.

Techniques:

Journal: Medical Microbiology and Immunology

Article Title: LDL acts as an opsonin enhancing the phagocytosis of group A Streptococcus by monocyte and whole human blood

doi: 10.1007/s00430-015-0436-8

Figure Lengend Snippet: Characteristics of GAS strains

Article Snippet: M28 (ATCC BAA1064) , + , + [ ] .

Techniques: Expressing, Binding Assay

Journal: Medical Microbiology and Immunology

Article Title: LDL acts as an opsonin enhancing the phagocytosis of group A Streptococcus by monocyte and whole human blood

doi: 10.1007/s00430-015-0436-8

Figure Lengend Snippet: LDL-mediated phagocytosis of four GAS strains. After 30-min incubation of U937 cells with FITC-labeled GAS DAPI was added and co-cultivated for 10 min; 5 ml of sample was plated onto slides, and GAS ( green ) and DAPI-stained U937 cell nuclei ( blue ) were imaged, respectively, at ×200 magnification on fluorescent microscope. a1 , b1 , c1 and d1 refer to control group, including AM41, CM41, M28 and M6-type GAS without LDL, whereas a2 , b2 , c2 and d2 stand for the treatments with LDL. The colony-forming units (cfu) of GAS in the mixture were determined by plating diluted samples onto THY agar plates after 15-, 30- and 60-min co-culture of FITC-GAS and U937 cells in the presence or absence of LDL. The phagocytosis rates were expressed as 100 % × (cfu of addition − cfu after co-culture)/cfu of addition. The red lines in a3 , b3 , c3 and d3 refer to the phagocytosis rates calculated upon cfu. Bacterial fluorescence intensity was monitored at 15, 30 and 60 min of incubation, and 200 µl of sample was pipetted into the 96-well microplate and measured at λ = 495 nm (excitation) and λ = 519 nm (emission) using a fluorescence plate reader. The phagocytosis rates were expressed as 100 % × (initial absorbance value − absorbance value after co-culture)/initial absorbance value. The green lines in a3 , b3 , c3 and d3 refer to the phagocytosis rates calculated upon fluorescence (fluor)

Article Snippet: M28 (ATCC BAA1064) , + , + [ ] .

Techniques: Incubation, Labeling, Staining, Microscopy, Control, Co-Culture Assay, Fluorescence

Journal: PLoS ONE

Article Title: Serotype M3 and M28 Group A Streptococci Have Distinct Capacities to Evade Neutrophil and TNF-α Responses and to Invade Soft Tissues

doi: 10.1371/journal.pone.0129417

Figure Lengend Snippet: Group A Streptococcus strains and plasmids used in this study.

Article Snippet: Serotype M3 GAS strain MGAS315 [ ] and serotype M1 strain MGAS2221 [ ] have been described, and serotype M28 strain MGAS6180 [ ] was obtained from American Type Culture Collection (ATCC No. BAA1064).

Techniques: Mutagenesis, Plasmid Preparation, Clone Assay, Knock-In

Journal: PLoS ONE

Article Title: Serotype M3 and M28 Group A Streptococci Have Distinct Capacities to Evade Neutrophil and TNF-α Responses and to Invade Soft Tissues

doi: 10.1371/journal.pone.0129417

Figure Lengend Snippet: MGAS315, MGAS2221, and MGAS6180 and their derivative strains were cultured overnight in THY, and SpeB activity in the supernatant of the cultures were detected by the casein hydrolysis plate assay.

Article Snippet: Serotype M3 GAS strain MGAS315 [ ] and serotype M1 strain MGAS2221 [ ] have been described, and serotype M28 strain MGAS6180 [ ] was obtained from American Type Culture Collection (ATCC No. BAA1064).

Techniques: Cell Culture, Activity Assay

Journal: PLoS ONE

Article Title: Serotype M3 and M28 Group A Streptococci Have Distinct Capacities to Evade Neutrophil and TNF-α Responses and to Invade Soft Tissues

doi: 10.1371/journal.pone.0129417

Figure Lengend Snippet: (A to E) Data in subcutaneous infection of mice with 0.2 ml MGAS315 (1.7 x 10 8 cfu) and MGAS6180 (1.1 x 10 8 cfu) suspensions at OD 600 of 1.0: Survival curves of 10 mice per group (A); Representative inside-out images of skin infection sites for MGAS315 at the endpoint when death was eminent (B) and for MGAS6180 at the endpoint, day 12 after inoculation (C) in which the arrow indicates the hole left by infection-caused loss of the skin; Areas of skin lesions and holes at the endpoints for MGAS315 and MGAS6180 infections (D); Loads of MGAS315 and MGAS6180 in the spleen and liver at the endpoints (solid symbols) (E). Panel E also includes GAS loads at day 1 after inoculation of 8.0 x 10 7 cfu MGAS315 and 1.1 x 10 8 cfu MGAS6180 in a separate infection experiment (open symbols). (G and F) MGAS315 and MGAS6180 loads in the spleen (G) and liver (F) within hours after inoculation of 1.9 x 10 8 cfu MGAS315 and 3.5 x 10 8 cfu MGAS6180.

Article Snippet: Serotype M3 GAS strain MGAS315 [ ] and serotype M1 strain MGAS2221 [ ] have been described, and serotype M28 strain MGAS6180 [ ] was obtained from American Type Culture Collection (ATCC No. BAA1064).

Techniques: Infection

Journal: PLoS ONE

Article Title: Serotype M3 and M28 Group A Streptococci Have Distinct Capacities to Evade Neutrophil and TNF-α Responses and to Invade Soft Tissues

doi: 10.1371/journal.pone.0129417

Figure Lengend Snippet: (A) Levels of neutrophils at skin infection sites in subcutaneous infections with 1.1 x 10 8 cfu MGAS315 and 1.4 x 10 8 cfu MGAS6180. (B-F) Data in the cytospin analysis of neutrophils infiltration in air sac infections with 6.6 x 10 7 MGAS315 and 1.1 x 10 8 cfu MGAS6180: Mean numbers ± error of viable neutrophils in the air sac lavages at the indicated times after GAS inoculation (B) and representative pictures of cytospin slides (C, no dilution of the lavage sample for cytospin; D-F, 2x dilution of the lavage samples for cytospin) comparing relative amounts of intact neutrophils versus cell debris at 4 h and 12 h in MGAS315 and MGAS6180 infections. *, P < 0.05, and **, P < 0.01, for the difference in neutrophil numbers between MGAS315 and MGAS6180 infections at 8 and 12 h, respectively.

Article Snippet: Serotype M3 GAS strain MGAS315 [ ] and serotype M1 strain MGAS2221 [ ] have been described, and serotype M28 strain MGAS6180 [ ] was obtained from American Type Culture Collection (ATCC No. BAA1064).

Techniques: Infection

Journal: PLoS ONE

Article Title: Serotype M3 and M28 Group A Streptococci Have Distinct Capacities to Evade Neutrophil and TNF-α Responses and to Invade Soft Tissues

doi: 10.1371/journal.pone.0129417

Figure Lengend Snippet: Presented are the levels of TNF-α (A), INF-γ (B), IL-6 (C), and IL-1β (D) at skin infection sites and levels of IL-6 and TNF-α (E) and INF-γ (F) in sera at the indicated time points after subcutaneous inoculation of 2.1 x 10 8 cfu MGAS315 and 2.7 x 10 8 cfu MGAS6180. Statistical analyses with the two-tailed Mann-Whitney t test for the cytokine levels between MGAS315 and MGAS6180 infections: ns, not significant; ***, P < 0.001; **, P < 0.01; and *, P < 0.05.

Article Snippet: Serotype M3 GAS strain MGAS315 [ ] and serotype M1 strain MGAS2221 [ ] have been described, and serotype M28 strain MGAS6180 [ ] was obtained from American Type Culture Collection (ATCC No. BAA1064).

Techniques: Infection, Two Tailed Test, MANN-WHITNEY

Journal: PLoS ONE

Article Title: Serotype M3 and M28 Group A Streptococci Have Distinct Capacities to Evade Neutrophil and TNF-α Responses and to Invade Soft Tissues

doi: 10.1371/journal.pone.0129417

Figure Lengend Snippet: (A) Relative levels of hasA , spyCEP , sse , and emm transcripts in MGAS315, MGAS315 Δ covS , MGAS6180, and MGAS6180 Δ covS , MGAS6180/pDCBB- covS wt , and MGAS6180/pDCBB. All the mRNA levels were normalized first to that of gyrA and then to that of each gene transcript in MGAS315. (B) Lesion sizes in subcutaneous infections of mice at 24 h after inoculation after inoculation with 1.3 x 10 8 cfu MGAS315 covS wt , 1.2 x 10 8 cfu MGAS315 Δ covS , 1.4 x 10 8 cfu MGAS6180, and 1.3 x 10 8 cfu MGAS6180 Δ covS . Statistical analysis with the One-way ANOVA Newman-Keuls Multiple Comparison Test in panel B: **, P < 0.01 and ***, P < 0.001.

Article Snippet: Serotype M3 GAS strain MGAS315 [ ] and serotype M1 strain MGAS2221 [ ] have been described, and serotype M28 strain MGAS6180 [ ] was obtained from American Type Culture Collection (ATCC No. BAA1064).

Techniques: Comparison