BAA-1064 Search Results


m28  (ATCC)
91
ATCC m28
Characteristics of GAS strains
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Characteristics of GAS strains

Journal: Medical Microbiology and Immunology

Article Title: LDL acts as an opsonin enhancing the phagocytosis of group A Streptococcus by monocyte and whole human blood

doi: 10.1007/s00430-015-0436-8

Figure Lengend Snippet: Characteristics of GAS strains

Article Snippet: M28 (ATCC BAA1064) , + , + [ ] .

Techniques: Expressing, Binding Assay

LDL-mediated phagocytosis of four GAS strains. After 30-min incubation of U937 cells with FITC-labeled GAS DAPI was added and co-cultivated for 10 min; 5 ml of sample was plated onto slides, and GAS ( green ) and DAPI-stained U937 cell nuclei ( blue ) were imaged, respectively, at ×200 magnification on fluorescent microscope. a1 , b1 , c1 and d1 refer to control group, including AM41, CM41, M28 and M6-type GAS without LDL, whereas a2 , b2 , c2 and d2 stand for the treatments with LDL. The colony-forming units (cfu) of GAS in the mixture were determined by plating diluted samples onto THY agar plates after 15-, 30- and 60-min co-culture of FITC-GAS and U937 cells in the presence or absence of LDL. The phagocytosis rates were expressed as 100 % × (cfu of addition − cfu after co-culture)/cfu of addition. The red lines in a3 , b3 , c3 and d3 refer to the phagocytosis rates calculated upon cfu. Bacterial fluorescence intensity was monitored at 15, 30 and 60 min of incubation, and 200 µl of sample was pipetted into the 96-well microplate and measured at λ = 495 nm (excitation) and λ = 519 nm (emission) using a fluorescence plate reader. The phagocytosis rates were expressed as 100 % × (initial absorbance value − absorbance value after co-culture)/initial absorbance value. The green lines in a3 , b3 , c3 and d3 refer to the phagocytosis rates calculated upon fluorescence (fluor)

Journal: Medical Microbiology and Immunology

Article Title: LDL acts as an opsonin enhancing the phagocytosis of group A Streptococcus by monocyte and whole human blood

doi: 10.1007/s00430-015-0436-8

Figure Lengend Snippet: LDL-mediated phagocytosis of four GAS strains. After 30-min incubation of U937 cells with FITC-labeled GAS DAPI was added and co-cultivated for 10 min; 5 ml of sample was plated onto slides, and GAS ( green ) and DAPI-stained U937 cell nuclei ( blue ) were imaged, respectively, at ×200 magnification on fluorescent microscope. a1 , b1 , c1 and d1 refer to control group, including AM41, CM41, M28 and M6-type GAS without LDL, whereas a2 , b2 , c2 and d2 stand for the treatments with LDL. The colony-forming units (cfu) of GAS in the mixture were determined by plating diluted samples onto THY agar plates after 15-, 30- and 60-min co-culture of FITC-GAS and U937 cells in the presence or absence of LDL. The phagocytosis rates were expressed as 100 % × (cfu of addition − cfu after co-culture)/cfu of addition. The red lines in a3 , b3 , c3 and d3 refer to the phagocytosis rates calculated upon cfu. Bacterial fluorescence intensity was monitored at 15, 30 and 60 min of incubation, and 200 µl of sample was pipetted into the 96-well microplate and measured at λ = 495 nm (excitation) and λ = 519 nm (emission) using a fluorescence plate reader. The phagocytosis rates were expressed as 100 % × (initial absorbance value − absorbance value after co-culture)/initial absorbance value. The green lines in a3 , b3 , c3 and d3 refer to the phagocytosis rates calculated upon fluorescence (fluor)

Article Snippet: M28 (ATCC BAA1064) , + , + [ ] .

Techniques: Incubation, Labeling, Staining, Microscopy, Control, Co-Culture Assay, Fluorescence