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yp 608147 1 pseudomonas entomophila l48 0 0 major facilitator transporter 3754 3795 4253450  (ATCC)


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    ATCC yp 608147 1 pseudomonas entomophila l48 0 0 major facilitator transporter 3754 3795 4253450
    Yp 608147 1 Pseudomonas Entomophila L48 0 0 Major Facilitator Transporter 3754 3795 4253450, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    yp 608147 1 pseudomonas entomophila l48 0 0 major facilitator transporter 3754 3795 4253450  (ATCC)
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    ATCC yp 608147 1 pseudomonas entomophila l48 0 0 major facilitator transporter 3754 3795 4253450
    Yp 608147 1 Pseudomonas Entomophila L48 0 0 Major Facilitator Transporter 3754 3795 4253450, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Combinatorial cloning of pathway modules into Destination vector I. a Combinatorial cloning of pathway genes into Destination vector I. The libraries of PCR-amplified (i) Pro HIS3 -ATF/BS-CDS2-Pro AmpR -AmpR-Ter AmpR (primers X1_for/Y1_rev, on Acceptor vector A), (ii) Pro LEU2 -ATF/BS-CDS3-Pro CmR -CmR-Ter CmR (primers X1_for/Y2_rev, on Acceptor vector B), (iii) Pro TRP1 -ATF/BS-CDS4-Pro <t>TCSR</t> -TCSR-Ter TCSR (primers X1_for/Y3_rev, on Acceptor vector C), and (iv) Pro LYS2 -ATF/BS-CDS5-Pro GenR -GenR-Ter GenR (primers X1_for/Y4_rev, on Acceptor vector D) modules are successively assembled in sites p2 – p5 , correspondingly, starting with the Destination vectors I-CDS1 library (see Fig. ), in four rounds of combinatorial cloning. b The COMPASS workflow for combinatorial assembly of ATF/BS and gene modules into Destination vector I. The mixed ATF/BS-CDS2 modules are assembled using TAR in site p2 of Destination vectors I-CDS1. Yeast cells with successful constructs grow on SC-Ura/-His medium. Cells are scraped from the plates, the plasmid library is extracted to obtain a pool of all randomized members, transformed into E . coli , and cells are grown on LB plates containing ampicillin. Cells are scraped from the plates to extract the plasmid library (1). The ATF/BS-CDS3 modules are assembled in site p3 of the Destination vectors I-CDS1-CDS2 library. Yeast cells with successful constructs grow on SC-Ura/-His/-Leu medium (2). The libraries of ATF/BS-CDS4 and ATF/BS-CDS5 modules are cloned into sites p4 and p5 , respectively (3 and 4). Gray arrows, nine ATF/BS units. Blue squares, iii: Asi SI, a: I-Ceu I, b: I-Sce I, c: PI-Psp I, d: PI-Sce I / Asc I. For simplicity, the IPTG-inducible promoters and terminators are not included in the figure. X0 and X1 are explained in footnote to Supplementary Data . Y1– Y4 overlap with the last 30 bp of terminators of the Amp R , Cmr R <t>,</t> <t>TCS</t> R , and Gen R genes
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    Combinatorial cloning of pathway modules into Destination vector I. a Combinatorial cloning of pathway genes into Destination vector I. The libraries of PCR-amplified (i) Pro HIS3 -ATF/BS-CDS2-Pro AmpR -AmpR-Ter AmpR (primers X1_for/Y1_rev, on Acceptor vector A), (ii) Pro LEU2 -ATF/BS-CDS3-Pro CmR -CmR-Ter CmR (primers X1_for/Y2_rev, on Acceptor vector B), (iii) Pro TRP1 -ATF/BS-CDS4-Pro <t>TCSR</t> -TCSR-Ter TCSR (primers X1_for/Y3_rev, on Acceptor vector C), and (iv) Pro LYS2 -ATF/BS-CDS5-Pro GenR -GenR-Ter GenR (primers X1_for/Y4_rev, on Acceptor vector D) modules are successively assembled in sites p2 – p5 , correspondingly, starting with the Destination vectors I-CDS1 library (see Fig. ), in four rounds of combinatorial cloning. b The COMPASS workflow for combinatorial assembly of ATF/BS and gene modules into Destination vector I. The mixed ATF/BS-CDS2 modules are assembled using TAR in site p2 of Destination vectors I-CDS1. Yeast cells with successful constructs grow on SC-Ura/-His medium. Cells are scraped from the plates, the plasmid library is extracted to obtain a pool of all randomized members, transformed into E . coli , and cells are grown on LB plates containing ampicillin. Cells are scraped from the plates to extract the plasmid library (1). The ATF/BS-CDS3 modules are assembled in site p3 of the Destination vectors I-CDS1-CDS2 library. Yeast cells with successful constructs grow on SC-Ura/-His/-Leu medium (2). The libraries of ATF/BS-CDS4 and ATF/BS-CDS5 modules are cloned into sites p4 and p5 , respectively (3 and 4). Gray arrows, nine ATF/BS units. Blue squares, iii: Asi SI, a: I-Ceu I, b: I-Sce I, c: PI-Psp I, d: PI-Sce I / Asc I. For simplicity, the IPTG-inducible promoters and terminators are not included in the figure. X0 and X1 are explained in footnote to Supplementary Data . Y1– Y4 overlap with the last 30 bp of terminators of the Amp R , Cmr R <t>,</t> <t>TCS</t> R , and Gen R genes
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    fuchsin chroma n a 42520 acid fuchsin em science cr 32 42685 safranin o mc b  (Millipore)
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    Combinatorial cloning of pathway modules into Destination vector I. a Combinatorial cloning of pathway genes into Destination vector I. The libraries of PCR-amplified (i) Pro HIS3 -ATF/BS-CDS2-Pro AmpR -AmpR-Ter AmpR (primers X1_for/Y1_rev, on Acceptor vector A), (ii) Pro LEU2 -ATF/BS-CDS3-Pro CmR -CmR-Ter CmR (primers X1_for/Y2_rev, on Acceptor vector B), (iii) Pro TRP1 -ATF/BS-CDS4-Pro <t>TCSR</t> -TCSR-Ter TCSR (primers X1_for/Y3_rev, on Acceptor vector C), and (iv) Pro LYS2 -ATF/BS-CDS5-Pro GenR -GenR-Ter GenR (primers X1_for/Y4_rev, on Acceptor vector D) modules are successively assembled in sites p2 – p5 , correspondingly, starting with the Destination vectors I-CDS1 library (see Fig. ), in four rounds of combinatorial cloning. b The COMPASS workflow for combinatorial assembly of ATF/BS and gene modules into Destination vector I. The mixed ATF/BS-CDS2 modules are assembled using TAR in site p2 of Destination vectors I-CDS1. Yeast cells with successful constructs grow on SC-Ura/-His medium. Cells are scraped from the plates, the plasmid library is extracted to obtain a pool of all randomized members, transformed into E . coli , and cells are grown on LB plates containing ampicillin. Cells are scraped from the plates to extract the plasmid library (1). The ATF/BS-CDS3 modules are assembled in site p3 of the Destination vectors I-CDS1-CDS2 library. Yeast cells with successful constructs grow on SC-Ura/-His/-Leu medium (2). The libraries of ATF/BS-CDS4 and ATF/BS-CDS5 modules are cloned into sites p4 and p5 , respectively (3 and 4). Gray arrows, nine ATF/BS units. Blue squares, iii: Asi SI, a: I-Ceu I, b: I-Sce I, c: PI-Psp I, d: PI-Sce I / Asc I. For simplicity, the IPTG-inducible promoters and terminators are not included in the figure. X0 and X1 are explained in footnote to Supplementary Data . Y1– Y4 overlap with the last 30 bp of terminators of the Amp R , Cmr R <t>,</t> <t>TCS</t> R , and Gen R genes
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    Combinatorial cloning of pathway modules into Destination vector I. a Combinatorial cloning of pathway genes into Destination vector I. The libraries of PCR-amplified (i) Pro HIS3 -ATF/BS-CDS2-Pro AmpR -AmpR-Ter AmpR (primers X1_for/Y1_rev, on Acceptor vector A), (ii) Pro LEU2 -ATF/BS-CDS3-Pro CmR -CmR-Ter CmR (primers X1_for/Y2_rev, on Acceptor vector B), (iii) Pro TRP1 -ATF/BS-CDS4-Pro <t>TCSR</t> -TCSR-Ter TCSR (primers X1_for/Y3_rev, on Acceptor vector C), and (iv) Pro LYS2 -ATF/BS-CDS5-Pro GenR -GenR-Ter GenR (primers X1_for/Y4_rev, on Acceptor vector D) modules are successively assembled in sites p2 – p5 , correspondingly, starting with the Destination vectors I-CDS1 library (see Fig. ), in four rounds of combinatorial cloning. b The COMPASS workflow for combinatorial assembly of ATF/BS and gene modules into Destination vector I. The mixed ATF/BS-CDS2 modules are assembled using TAR in site p2 of Destination vectors I-CDS1. Yeast cells with successful constructs grow on SC-Ura/-His medium. Cells are scraped from the plates, the plasmid library is extracted to obtain a pool of all randomized members, transformed into E . coli , and cells are grown on LB plates containing ampicillin. Cells are scraped from the plates to extract the plasmid library (1). The ATF/BS-CDS3 modules are assembled in site p3 of the Destination vectors I-CDS1-CDS2 library. Yeast cells with successful constructs grow on SC-Ura/-His/-Leu medium (2). The libraries of ATF/BS-CDS4 and ATF/BS-CDS5 modules are cloned into sites p4 and p5 , respectively (3 and 4). Gray arrows, nine ATF/BS units. Blue squares, iii: Asi SI, a: I-Ceu I, b: I-Sce I, c: PI-Psp I, d: PI-Sce I / Asc I. For simplicity, the IPTG-inducible promoters and terminators are not included in the figure. X0 and X1 are explained in footnote to Supplementary Data . Y1– Y4 overlap with the last 30 bp of terminators of the Amp R , Cmr R <t>,</t> <t>TCS</t> R , and Gen R genes
    Model 42520 Ir Thermometer, supplied by Extech Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Combinatorial cloning of pathway modules into Destination vector I. a Combinatorial cloning of pathway genes into Destination vector I. The libraries of PCR-amplified (i) Pro HIS3 -ATF/BS-CDS2-Pro AmpR -AmpR-Ter AmpR (primers X1_for/Y1_rev, on Acceptor vector A), (ii) Pro LEU2 -ATF/BS-CDS3-Pro CmR -CmR-Ter CmR (primers X1_for/Y2_rev, on Acceptor vector B), (iii) Pro TRP1 -ATF/BS-CDS4-Pro TCSR -TCSR-Ter TCSR (primers X1_for/Y3_rev, on Acceptor vector C), and (iv) Pro LYS2 -ATF/BS-CDS5-Pro GenR -GenR-Ter GenR (primers X1_for/Y4_rev, on Acceptor vector D) modules are successively assembled in sites p2 – p5 , correspondingly, starting with the Destination vectors I-CDS1 library (see Fig. ), in four rounds of combinatorial cloning. b The COMPASS workflow for combinatorial assembly of ATF/BS and gene modules into Destination vector I. The mixed ATF/BS-CDS2 modules are assembled using TAR in site p2 of Destination vectors I-CDS1. Yeast cells with successful constructs grow on SC-Ura/-His medium. Cells are scraped from the plates, the plasmid library is extracted to obtain a pool of all randomized members, transformed into E . coli , and cells are grown on LB plates containing ampicillin. Cells are scraped from the plates to extract the plasmid library (1). The ATF/BS-CDS3 modules are assembled in site p3 of the Destination vectors I-CDS1-CDS2 library. Yeast cells with successful constructs grow on SC-Ura/-His/-Leu medium (2). The libraries of ATF/BS-CDS4 and ATF/BS-CDS5 modules are cloned into sites p4 and p5 , respectively (3 and 4). Gray arrows, nine ATF/BS units. Blue squares, iii: Asi SI, a: I-Ceu I, b: I-Sce I, c: PI-Psp I, d: PI-Sce I / Asc I. For simplicity, the IPTG-inducible promoters and terminators are not included in the figure. X0 and X1 are explained in footnote to Supplementary Data . Y1– Y4 overlap with the last 30 bp of terminators of the Amp R , Cmr R , TCS R , and Gen R genes

    Journal: Nature Communications

    Article Title: COMPASS for rapid combinatorial optimization of biochemical pathways based on artificial transcription factors

    doi: 10.1038/s41467-019-10224-x

    Figure Lengend Snippet: Combinatorial cloning of pathway modules into Destination vector I. a Combinatorial cloning of pathway genes into Destination vector I. The libraries of PCR-amplified (i) Pro HIS3 -ATF/BS-CDS2-Pro AmpR -AmpR-Ter AmpR (primers X1_for/Y1_rev, on Acceptor vector A), (ii) Pro LEU2 -ATF/BS-CDS3-Pro CmR -CmR-Ter CmR (primers X1_for/Y2_rev, on Acceptor vector B), (iii) Pro TRP1 -ATF/BS-CDS4-Pro TCSR -TCSR-Ter TCSR (primers X1_for/Y3_rev, on Acceptor vector C), and (iv) Pro LYS2 -ATF/BS-CDS5-Pro GenR -GenR-Ter GenR (primers X1_for/Y4_rev, on Acceptor vector D) modules are successively assembled in sites p2 – p5 , correspondingly, starting with the Destination vectors I-CDS1 library (see Fig. ), in four rounds of combinatorial cloning. b The COMPASS workflow for combinatorial assembly of ATF/BS and gene modules into Destination vector I. The mixed ATF/BS-CDS2 modules are assembled using TAR in site p2 of Destination vectors I-CDS1. Yeast cells with successful constructs grow on SC-Ura/-His medium. Cells are scraped from the plates, the plasmid library is extracted to obtain a pool of all randomized members, transformed into E . coli , and cells are grown on LB plates containing ampicillin. Cells are scraped from the plates to extract the plasmid library (1). The ATF/BS-CDS3 modules are assembled in site p3 of the Destination vectors I-CDS1-CDS2 library. Yeast cells with successful constructs grow on SC-Ura/-His/-Leu medium (2). The libraries of ATF/BS-CDS4 and ATF/BS-CDS5 modules are cloned into sites p4 and p5 , respectively (3 and 4). Gray arrows, nine ATF/BS units. Blue squares, iii: Asi SI, a: I-Ceu I, b: I-Sce I, c: PI-Psp I, d: PI-Sce I / Asc I. For simplicity, the IPTG-inducible promoters and terminators are not included in the figure. X0 and X1 are explained in footnote to Supplementary Data . Y1– Y4 overlap with the last 30 bp of terminators of the Amp R , Cmr R , TCS R , and Gen R genes

    Article Snippet: The resulting plasmid was named Acceptor vector F. (iii) Neo and Ter neo (primers KANARTER_for/KANARTER_rev, on pCEV-G2-Km, Addgene #46815), Pro TDH3 (primers TDHG_for/TDHG_rev, on pCEV-G2-Km, Addgene #46815), TCS R and Ter TCSR (primers, on TCSRGTER_for/TSCRGTER_rev, on pF2, Addgene #42520) to result in pCOMPASS17.

    Techniques: Clone Assay, Plasmid Preparation, Amplification, Construct, Transformation Assay