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genex plus transfection reagent  (ATCC)


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    Structured Review

    ATCC genex plus transfection reagent
    Genex Plus Transfection Reagent, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4004/GeneXPlus+Transfection+Reagent/pmc12921692-111-27-31
    Average 95 stars, based on 36 article reviews
    genex plus transfection reagent - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Opposing roles of p38α-mediated phosphorylation and PRMT1-mediated arginine methylation in driving TDP-43 proteinopathy
    Article Snippet: LDH activity was measured from 50μL of conditioned medium using LDH assay kit (Life Technologies) following the manufacturer’s instructions. .. SH-SY5Y cells were cultured and transiently transfected (using GeneX Plus transfection reagent (ATCC)) with 0.5μg of p38α constructs or GFP control construct according to manufacturer’s protocol. ..

    Transfection:

    Article Title: Opposing roles of p38α-mediated phosphorylation and PRMT1-mediated arginine methylation in driving TDP-43 proteinopathy
    Article Snippet: LDH activity was measured from 50μL of conditioned medium using LDH assay kit (Life Technologies) following the manufacturer’s instructions. .. SH-SY5Y cells were cultured and transiently transfected (using GeneX Plus transfection reagent (ATCC)) with 0.5μg of p38α constructs or GFP control construct according to manufacturer’s protocol. ..

    Article Title: Elucidation of Molecular Mechanisms of Lipid-Altered Cytotoxicity of TDP-43 Fibrils.
    Article Snippet: Imaging was performed using the EVOS M5000 Imaging System (Thermo Fisher Scientific, USA) equipped with an Olympus UPlanApo 100x/1.35 oil iris ∞/0.17 objective and blue, green, and deep red filters. .. To assess membrane integrity and autophagic responses, N27 cells were transfected with plasmids coding for Chmp1b (membrane repair), Gal3 (autophagy marker), and TFEB (lysosomal biogenesis regulator) using GeneX Plus transfection reagent (ATCC, cat. no. ACS-4004). .. To assess membrane integrity and autophagic responses, N27 cells were transfected with plasmids coding for Chmp1b (membrane repair), Gal3 (autophagy marker), and TFEB (lysosomal biogenesis regulator) using GeneX Plus transfection reagent (ATCC, cat. no. ACS-4004).

    Article Title: Opposing roles of p38α-mediated phosphorylation and PRMT1-mediated arginine methylation in driving TDP-43 proteinopathy
    Article Snippet: .. Cells were transiently transfected using GeneX Plus transfection reagent (ATCC) folllowing manufacturer’s protocol. ..

    Article Title: Alpha-synuclein mutations mislocalize cytoplasmic p300 compromising autophagy, which is rescued by ACLY inhibition.
    Article Snippet: Drug treatments include: DMSO, 10 mM NAM (#N0636), 2 mM TSA (#T8552), 10 mM sodium butyrate (#B5887), 10 mM NH4Cl (#A9434), 50 mM – 1 mM potassium hydroxycitrate tribasic monohydrate (HC; #59847) from Sigma-Aldrich; 2 mM A485 (#6387), 1- 10 mM A 769662 (#3336/10), 0.25 mM AICAR (#2840), 10-20 mM SB 204990 (#4962), 10 mM Akti-1/2 (#5773), 1 mM KT 5720 (#1288) from Tocris; 5 mMcompound C (#171260) fromMerck; 10 mM991 (#AOB8150) from AOBIOUS Inc.; 400-500 mg/ml Geneticin Selective Antibiotic (G418; #11811-031) from Gibco; 400 nM BafA1 from Enzo Life Sciences; 20 mM SB 415286 (#A8241) from ApexBio; 10-25 mM BMS-303141 (#HY-16107) from Med Chem Express; 10 mM 991 (#S8654) from Selleck Chemicals; MEM amino acids solution (#11130-051), Non-essential amino acids solution (#11140-050) from Thermo Fisher Scientific. .. TransIT-2020 reagent (#MIR5400; Mirus) was used for DNA transfection of most cell lines, while GeneXPlus reagent (#ACS-4004; ATCC) was used for SH-SY5Y cells according to the manufacturer’s instructions. .. Lipofectamine 2000 (#11668) or Lipofectamine RNAiMAX (#13778) (Invitrogen) was used for siRNA transfections, according to the manufacturer’s instructions.

    Article Title: Neurodevelopmental SHQ1 variants navigate the control of apoptosis, endoplasmic reticulum stress, and oxidative stress in neuroblastoma cells.
    Article Snippet: SHQ1 binding to dyskerin/NAP57 is crucial for the assembly of H/ACA small nucleolar ribonucleoproteins (snoRNPs), which play essential roles in ribosome biogenesis and spliceosome function.. Additionally, SHQ1 has been implicated in tumor suppression by interacting with the endoplasmic reticulum (ER) chaperone glucoseregulated protein 78 (GRP78), thereby promoting apoptosis.. Recent studies have identified biallelic SHQ1 causative variants in patients with neurodevelopment disorders, however, their impact on the tumor remains unclear.

    Article Title: Elucidation of Molecular Mechanisms of Lipid-Altered Cytotoxicity of TDP-43 Fibrils
    Article Snippet: .. To assess membrane integrity and autophagic responses, N27 cells were transfected with plasmids coding for Chmp1b (membrane repair), Gal3 (autophagy marker), and TFEB (lysosomal biogenesis regulator) using GeneX Plus transfection reagent (ATCC, cat. no. ACS-4004). .. After cell reached ∼ 70% confluency, transfection was performed in serum-free DMEM (Corning, cat. no. 10-013) for 8 h. Next, TDP-43 aggregates were added to the cells and incubated for 24 h at 37 °C in a 5% CO 2 .

    Article Title: Opposing roles of p38α-mediated phosphorylation and PRMT1-mediated arginine methylation in driving TDP-43 proteinopathy
    Article Snippet: Dextran from Leuconostoc spp , Millipore Sigma , Cat #31392. .. GeneXPlus Transfection Reagent , ATCC , ACS-4004. .. Formaldehyde solution (37%) , Sigma , 252549.

    Construct:

    Article Title: Opposing roles of p38α-mediated phosphorylation and PRMT1-mediated arginine methylation in driving TDP-43 proteinopathy
    Article Snippet: LDH activity was measured from 50μL of conditioned medium using LDH assay kit (Life Technologies) following the manufacturer’s instructions. .. SH-SY5Y cells were cultured and transiently transfected (using GeneX Plus transfection reagent (ATCC)) with 0.5μg of p38α constructs or GFP control construct according to manufacturer’s protocol. ..

    Control:

    Article Title: Opposing roles of p38α-mediated phosphorylation and PRMT1-mediated arginine methylation in driving TDP-43 proteinopathy
    Article Snippet: LDH activity was measured from 50μL of conditioned medium using LDH assay kit (Life Technologies) following the manufacturer’s instructions. .. SH-SY5Y cells were cultured and transiently transfected (using GeneX Plus transfection reagent (ATCC)) with 0.5μg of p38α constructs or GFP control construct according to manufacturer’s protocol. ..

    Membrane:

    Article Title: Elucidation of Molecular Mechanisms of Lipid-Altered Cytotoxicity of TDP-43 Fibrils.
    Article Snippet: Imaging was performed using the EVOS M5000 Imaging System (Thermo Fisher Scientific, USA) equipped with an Olympus UPlanApo 100x/1.35 oil iris ∞/0.17 objective and blue, green, and deep red filters. .. To assess membrane integrity and autophagic responses, N27 cells were transfected with plasmids coding for Chmp1b (membrane repair), Gal3 (autophagy marker), and TFEB (lysosomal biogenesis regulator) using GeneX Plus transfection reagent (ATCC, cat. no. ACS-4004). .. To assess membrane integrity and autophagic responses, N27 cells were transfected with plasmids coding for Chmp1b (membrane repair), Gal3 (autophagy marker), and TFEB (lysosomal biogenesis regulator) using GeneX Plus transfection reagent (ATCC, cat. no. ACS-4004).

    Article Title: Elucidation of Molecular Mechanisms of Lipid-Altered Cytotoxicity of TDP-43 Fibrils
    Article Snippet: .. To assess membrane integrity and autophagic responses, N27 cells were transfected with plasmids coding for Chmp1b (membrane repair), Gal3 (autophagy marker), and TFEB (lysosomal biogenesis regulator) using GeneX Plus transfection reagent (ATCC, cat. no. ACS-4004). .. After cell reached ∼ 70% confluency, transfection was performed in serum-free DMEM (Corning, cat. no. 10-013) for 8 h. Next, TDP-43 aggregates were added to the cells and incubated for 24 h at 37 °C in a 5% CO 2 .

    Marker:

    Article Title: Elucidation of Molecular Mechanisms of Lipid-Altered Cytotoxicity of TDP-43 Fibrils.
    Article Snippet: Imaging was performed using the EVOS M5000 Imaging System (Thermo Fisher Scientific, USA) equipped with an Olympus UPlanApo 100x/1.35 oil iris ∞/0.17 objective and blue, green, and deep red filters. .. To assess membrane integrity and autophagic responses, N27 cells were transfected with plasmids coding for Chmp1b (membrane repair), Gal3 (autophagy marker), and TFEB (lysosomal biogenesis regulator) using GeneX Plus transfection reagent (ATCC, cat. no. ACS-4004). .. To assess membrane integrity and autophagic responses, N27 cells were transfected with plasmids coding for Chmp1b (membrane repair), Gal3 (autophagy marker), and TFEB (lysosomal biogenesis regulator) using GeneX Plus transfection reagent (ATCC, cat. no. ACS-4004).

    Article Title: Elucidation of Molecular Mechanisms of Lipid-Altered Cytotoxicity of TDP-43 Fibrils
    Article Snippet: .. To assess membrane integrity and autophagic responses, N27 cells were transfected with plasmids coding for Chmp1b (membrane repair), Gal3 (autophagy marker), and TFEB (lysosomal biogenesis regulator) using GeneX Plus transfection reagent (ATCC, cat. no. ACS-4004). .. After cell reached ∼ 70% confluency, transfection was performed in serum-free DMEM (Corning, cat. no. 10-013) for 8 h. Next, TDP-43 aggregates were added to the cells and incubated for 24 h at 37 °C in a 5% CO 2 .



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