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rpmi 1640 medium  (ATCC)


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    ATCC rpmi 1640 medium
    Rpmi 1640 Medium, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 19610 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/30-2001/RPMI-1640+Medium/pm42781935-85-9-5
    Average 99 stars, based on 19610 article reviews
    rpmi 1640 medium - by Bioz Stars, 2026-10
    99/100 stars

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    Cell Culture:

    Article Title: Improving CTC isolation by controlling orbit engagement in inertial microfluidics.
    Article Snippet: .. A549 cells were obtained from ATCC and cultured in RPMI-1640 medium (Fisher Scientific, Waltham, MA, USA) supplemented with 10% (v/v) fetal bovine serum and 1% (v/v) antibiotic antimycotic solution. .. Both cell lines were maintained as adherent cultures at 37 °C with 5% CO2 and harvested at ∼80% confluency using TrypLE (1×; Fisher Scientific, Waltham, MA, USA).

    Article Title: Integrative machine learning and transcriptomic analysis reveal a ferroptosis gene signature for pediatric Mycoplasma pneumoniae pneumonia
    Article Snippet: .. In vitro experimental validation was performed using two ATCC-authenticated human cell lines relevant to the pulmonary immune microenvironment: THP-1 (ATCC TIB-202), a human monocytic cell line that was differentiated into macrophage-like cells by treatment with phorbol 12-myristate 13-acetate (PMA; 100 nM, 48 h) in RPMI-1640 medium (ATCC 30–2001) supplemented with 10% fetal bovine serum (FBS) and 0.05 mM 2-mercaptoethanol; THP-1 were cultured at 37 °C in a humidified atmosphere of 5% CO 2 . ..

    Article Title: Investigating Precursor Spatial Organization in Osteoclastogenesis Using DNA-Directed Cell Patterning.
    Article Snippet: .. THP-1 cells obtained from ATCC were cultured in RPMI 1640 medium supplemented with 10% Fetal Bovine Serum (FBS) (v/v) and 0.05 mM 2-mercaptoethanol. .. Cells were maintained in an incubator with 5% CO2 at 37 °C and cultured following ATCC recommendations.

    Article Title: PAG supports T cell immune synapse function by binding to actin.
    Article Snippet: Immunotherapies affect T cell function by modifying the immune synapse, which forms at the interface between T cells and their targets.. Phosphoprotein associated with glycosphingolipidrich microdomains 1 (PAG) is a T cell adaptor protein within the immune synapse that mediates signaling by the inhibitory coreceptor PD1.. Because PAG undergoes palmitoylation, which positions it within plasma membrane lipid rafts, and contains a carboxylterminal PDZbinding motif that connects to the actin cytoskeleton, PAG is a potential target for modulating immune function.

    Incubator:

    Article Title: MGST3 defines a mitochondrial-health-associated M2 macrophage state in atherosclerosis: integrative multi-omics, single-cell mediation, and functional validation
    Article Snippet: .. Human THP-1 monocytes (ATCC) were maintained in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin at 37 °C in a humidified 5% CO2 incubator. .. Cells were differentiated into macrophages by treatment with 100 ng/mL phorbol 12-myristate 13-acetate (PMA) for 48 h. After medium replacement, macrophages were transfected with 50 nM non-targeting control siRNA (siNC) or MGST3-targeting siRNA (siMGST3) using Lipofectamine RNAiMAX (Thermo Fisher Scientific) according to the manufacturer’s instructions.

    In Vitro:

    Article Title: Integrative machine learning and transcriptomic analysis reveal a ferroptosis gene signature for pediatric Mycoplasma pneumoniae pneumonia
    Article Snippet: .. In vitro experimental validation was performed using two ATCC-authenticated human cell lines relevant to the pulmonary immune microenvironment: THP-1 (ATCC TIB-202), a human monocytic cell line that was differentiated into macrophage-like cells by treatment with phorbol 12-myristate 13-acetate (PMA; 100 nM, 48 h) in RPMI-1640 medium (ATCC 30–2001) supplemented with 10% fetal bovine serum (FBS) and 0.05 mM 2-mercaptoethanol; THP-1 were cultured at 37 °C in a humidified atmosphere of 5% CO 2 . ..

    Biomarker Discovery:

    Article Title: Integrative machine learning and transcriptomic analysis reveal a ferroptosis gene signature for pediatric Mycoplasma pneumoniae pneumonia
    Article Snippet: .. In vitro experimental validation was performed using two ATCC-authenticated human cell lines relevant to the pulmonary immune microenvironment: THP-1 (ATCC TIB-202), a human monocytic cell line that was differentiated into macrophage-like cells by treatment with phorbol 12-myristate 13-acetate (PMA; 100 nM, 48 h) in RPMI-1640 medium (ATCC 30–2001) supplemented with 10% fetal bovine serum (FBS) and 0.05 mM 2-mercaptoethanol; THP-1 were cultured at 37 °C in a humidified atmosphere of 5% CO 2 . ..



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    rstAB contributes to UPEC CFT073 virulence by promoting its invasion of BECs (A) Total bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 24 hpi ( n = 9 mice). (B) Intracellular bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 1 hpi ( n = 9 mice). (C) Intracellular bacterial titers of WT, Δ rstAB , or Δ rstAB + P rstAB in <t>5637</t> cells at 1 hpi ( n = 3). (D) Fold changes in rstA and rstB mRNA levels in WT-infected BALB/c mouse bladders at 1 hpi compared to that of <t>WT</t> <t>cultured</t> in LB medium ( n = 3). (E) Fold changes in rstA and rstB mRNA levels in WT-infected 5637 cells at 1 hpi ( n = 3) compared to that of WT cultured in LB medium. (F) Total bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 6 hpi ( n = 9 mice). (G) IBC enumeration in C3H/HeN mouse bladders transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 6 hpi determined using confocal microscopy ( n = 9 mice). Data were obtained from three independent experiments and presented as mean ± SD. Significance was determined using two-tailed Mann-Whitney U test (A, B, F, and G) and two-tailed unpaired Student’s t test (C, D, and E). Significance was indicated by p value. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ＜0.0001; n.s. represents no significant difference. See also and .
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    rstAB contributes to UPEC CFT073 virulence by promoting its invasion of BECs (A) Total bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 24 hpi ( n = 9 mice). (B) Intracellular bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 1 hpi ( n = 9 mice). (C) Intracellular bacterial titers of WT, Δ rstAB , or Δ rstAB + P rstAB in 5637 cells at 1 hpi ( n = 3). (D) Fold changes in rstA and rstB mRNA levels in WT-infected BALB/c mouse bladders at 1 hpi compared to that of WT cultured in LB medium ( n = 3). (E) Fold changes in rstA and rstB mRNA levels in WT-infected 5637 cells at 1 hpi ( n = 3) compared to that of WT cultured in LB medium. (F) Total bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 6 hpi ( n = 9 mice). (G) IBC enumeration in C3H/HeN mouse bladders transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 6 hpi determined using confocal microscopy ( n = 9 mice). Data were obtained from three independent experiments and presented as mean ± SD. Significance was determined using two-tailed Mann-Whitney U test (A, B, F, and G) and two-tailed unpaired Student’s t test (C, D, and E). Significance was indicated by p value. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ＜0.0001; n.s. represents no significant difference. See also and .

    Journal: iScience

    Article Title: RstAB activates type 1 fimbriae to promote uropathogenic Escherichia coli bladder invasion

    doi: 10.1016/j.isci.2026.116333

    Figure Lengend Snippet: rstAB contributes to UPEC CFT073 virulence by promoting its invasion of BECs (A) Total bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 24 hpi ( n = 9 mice). (B) Intracellular bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 1 hpi ( n = 9 mice). (C) Intracellular bacterial titers of WT, Δ rstAB , or Δ rstAB + P rstAB in 5637 cells at 1 hpi ( n = 3). (D) Fold changes in rstA and rstB mRNA levels in WT-infected BALB/c mouse bladders at 1 hpi compared to that of WT cultured in LB medium ( n = 3). (E) Fold changes in rstA and rstB mRNA levels in WT-infected 5637 cells at 1 hpi ( n = 3) compared to that of WT cultured in LB medium. (F) Total bacterial titers of UPEC CFT073 in the bladders of BALB/c mice transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 6 hpi ( n = 9 mice). (G) IBC enumeration in C3H/HeN mouse bladders transurethrally infected with WT, Δ rstAB , or Δ rstAB + P rstAB at 6 hpi determined using confocal microscopy ( n = 9 mice). Data were obtained from three independent experiments and presented as mean ± SD. Significance was determined using two-tailed Mann-Whitney U test (A, B, F, and G) and two-tailed unpaired Student’s t test (C, D, and E). Significance was indicated by p value. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ＜0.0001; n.s. represents no significant difference. See also and .

    Article Snippet: 5637 cells (ATCC HTB-9) were purchased from American Type Culture Collection and cultured in RPMI 1640 medium (CELLMAX; JYC11875500BT) containing 10% fetal bovine serum (Gibco; 10091130) at 37 °C, 5% CO 2 , with 1% streptomycin and 1% penicillin as necessary.

    Techniques: Infection, Cell Culture, Confocal Microscopy, Two Tailed Test, MANN-WHITNEY

    RstAB activates the expression and production of type 1 fimbria (A) Real-time qPCR analyses of the mRNA levels of fimA , fimC , fimD , fimF , fimH , fimG , and fimI in WT, Δ rstAB , or Δ rstAB + P rstAB statically cultured in LB medium for 12 h. (B) Real-time qPCR analyses of the mRNA levels of fimA , fimC , fimD , fimF , fimH , fimG , and fimI in WT-, Δ rstAB- , or Δ rstAB + P rstAB -infected 5637 cells at 1 hpi. (C) Real-time qPCR analyses of the mRNA levels of fimA , fimC , fimD , fimF , fimH , fimG , and fimI in WT-, Δ rstAB -, or Δ rstAB + P rstAB -infected mouse bladders at 1 hpi. (D) Quantitative analysis of FimH protein levels in WT, Δ rstAB , or Δ rstAB + P rstAB statically grown in LB medium at 37°C overnight (top). DnaK served as a loading control. Representative image (bottom) from three independent experiments. (E) HA assays of the production of type 1 fimbria in WT, Δ rstAB , Δ rstAB + P rstAB , Δ fimA-H , or Δ fimAH Δ rstAB in the presence or absence of 3% mannose. Data were obtained from three independent experiments and presented as mean ± SD. Significance was determined by two-tailed unpaired Student’s t test (A–D) and two-way analysis of variance (E). Significance was indicated by a p value. ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ＜0.0001; n.s. no significant difference. See also and .

    Journal: iScience

    Article Title: RstAB activates type 1 fimbriae to promote uropathogenic Escherichia coli bladder invasion

    doi: 10.1016/j.isci.2026.116333

    Figure Lengend Snippet: RstAB activates the expression and production of type 1 fimbria (A) Real-time qPCR analyses of the mRNA levels of fimA , fimC , fimD , fimF , fimH , fimG , and fimI in WT, Δ rstAB , or Δ rstAB + P rstAB statically cultured in LB medium for 12 h. (B) Real-time qPCR analyses of the mRNA levels of fimA , fimC , fimD , fimF , fimH , fimG , and fimI in WT-, Δ rstAB- , or Δ rstAB + P rstAB -infected 5637 cells at 1 hpi. (C) Real-time qPCR analyses of the mRNA levels of fimA , fimC , fimD , fimF , fimH , fimG , and fimI in WT-, Δ rstAB -, or Δ rstAB + P rstAB -infected mouse bladders at 1 hpi. (D) Quantitative analysis of FimH protein levels in WT, Δ rstAB , or Δ rstAB + P rstAB statically grown in LB medium at 37°C overnight (top). DnaK served as a loading control. Representative image (bottom) from three independent experiments. (E) HA assays of the production of type 1 fimbria in WT, Δ rstAB , Δ rstAB + P rstAB , Δ fimA-H , or Δ fimAH Δ rstAB in the presence or absence of 3% mannose. Data were obtained from three independent experiments and presented as mean ± SD. Significance was determined by two-tailed unpaired Student’s t test (A–D) and two-way analysis of variance (E). Significance was indicated by a p value. ∗∗∗ p ≤ 0.001, ∗∗∗∗ p ＜0.0001; n.s. no significant difference. See also and .

    Article Snippet: 5637 cells (ATCC HTB-9) were purchased from American Type Culture Collection and cultured in RPMI 1640 medium (CELLMAX; JYC11875500BT) containing 10% fetal bovine serum (Gibco; 10091130) at 37 °C, 5% CO 2 , with 1% streptomycin and 1% penicillin as necessary.

    Techniques: Expressing, Cell Culture, Infection, Control, Two Tailed Test