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rpmi 1640 medium Rpmi 1640 Medium, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/30-2001/RPMI-1640+Medium/pmc02862930-42-15-2 Average 99 stars, based on 1 article reviews
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Image Search Results
Journal: eLife
Article Title: Functional membrane microdomains and the hydroxamate siderophore transporter ATPase FhuC govern Isd-dependent heme acquisition in Staphylococcus aureus
doi: 10.7554/eLife.85304
Figure Lengend Snippet: ( A–D ) Strains were grown in iron-limited medium (A: 100 µl in 96-well plates; B–D: 500 µl in 48-well plates) in the presence of 20 µM FeSO 4 . Cultures were inoculated to an OD 600 =0.005 and OD 600 was monitored every 15 min at 37°C orbital shaking using an Epoch2 plate reader. ( A ) Growth of S. aureus USA300 JE2 WT, Δ floA::Erm, and floA _Revertant ( floA_R ). For reasons of clarity, values after 24 hr are displayed. Means and SD of four experiments are shown. ( B ) Growth of S. aureus Newman WT, Δ isd , Δ floA, and Δ crtM mutants. Values after 16 hr are displayed. Means and SD of three experiments are shown. ( C,D ) Newman WT ( C ) and Δ fhuC ( D ) were grown in the presence of 10 µM zaragozic acid (ZA) and FeSO 4 . Values taken every 2 hr are displayed. Means and SD of four experiments are shown. Figure 5—figure supplement 1—source data 1. FeSO 4 growth controls of floA and functional membrane microdomain (FMM)-deficient mutants.
Article Snippet: Chemical compound, drug ,
Techniques: Functional Assay, Membrane
Journal: eLife
Article Title: Functional membrane microdomains and the hydroxamate siderophore transporter ATPase FhuC govern Isd-dependent heme acquisition in Staphylococcus aureus
doi: 10.7554/eLife.85304
Figure Lengend Snippet: ( A–D ) Strains were grown in iron-limited medium (A: 100 µl in 96-well plates; B–D: 500 µl in 48-well plates). ( A ) Growth of S. aureus USA300 JE2 WT, Δ floA::Erm and floA _Revertant ( floA_R ) in the presence of 1 µg/ml human hemoglobin (hHb). For reasons of clarity, values after 24 hr are displayed. Means and SD of four experiments are shown. ( B ) Growth of S. aureus Newman WT, Δ isd , Δ floA, and Δ crtM mutants. Strains were grown in the presence of 1 µg/ml hHb. Values after 16 hr are displayed. Means and SD of three experiments are shown. ( C,D ) Newman WT ( C ) and Δ fhuC ( D ) were grown in the presence of 10 µM zaragozic acid (ZA) and 2.5 µg/ml hHb. Values taken every 2 hr are displayed. Means and SD of four experiments are shown. ( A,B ) Statistical analysis: Student’s one-way ANOVA followed by Dunett’s test for multiple comparisons was performed using GraphPad Prism 9. ( C,D ) Statistical analysis: Student’s unpaired t-test was performed using GraphPad Prism 8. *p<0.05, **p<0.01, ***p<0.001. Figure 5—source data 1. Flotillin A (FloA) and functional membrane microdomains (FMMs) are needed for proliferation with hemoglobin.
Article Snippet: Chemical compound, drug ,
Techniques: Functional Assay, Membrane
Journal: eLife
Article Title: Functional membrane microdomains and the hydroxamate siderophore transporter ATPase FhuC govern Isd-dependent heme acquisition in Staphylococcus aureus
doi: 10.7554/eLife.85304
Figure Lengend Snippet:
Article Snippet: Chemical compound, drug ,
Techniques: Cloning, Mutagenesis, Plasmid Preparation, Control, Expressing, Transduction, Purification, Recombinant, Amplification, Positive Control, Sequencing, Construct, Protein Extraction, Fluorescence, Microscopy