acqb01000091 acinetobacter halotolerans r160 t kt032155 staphylococcus lentus atcc 29070 t (ATCC)
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acqb01000091 acinetobacter halotolerans r160 t kt032155 staphylococcus lentus atcc 29070 t
Acqb01000091 Acinetobacter Halotolerans R160 T Kt032155 Staphylococcus Lentus Atcc 29070 T, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 21658 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/29070/Staphylococcus/pm33030749-131-150-158
Average 99 stars, based on 21658 article reviews
Acqb01000091 Acinetobacter Halotolerans R160 T Kt032155 Staphylococcus Lentus Atcc 29070 T, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 21658 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/29070/Staphylococcus/pm33030749-131-150-158
Average 99 stars, based on 21658 article reviews
acqb01000091 acinetobacter halotolerans r160 t kt032155 staphylococcus lentus atcc 29070 t - by Bioz Stars,
2026-09
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Article Snippet: JOURNAL OF ZHEJIANG UNIVERSITY(MEDICAL SCIENCES) 浙 江 大 学 学 报( 医 学 版 ) XXXX 年 XX 月 XX XXXX 茯苓酸促进3T3-L1 MBX前脂肪细胞棕色 化成脂分化及脂代谢 陈坤铃,窦晓兵,林益游,白丹瑶,罗杨舟,周丽萍 浙江中医药大学生命科学学院,浙江 杭州 310053 [摘 要] 目的: 探究茯苓酸对 3T3-L1 MBX 前脂肪细胞棕色化成脂分化及脂代 谢的影响。方法: 采用棕色鸡尾酒法诱导 3T3-L1 MBX 前脂肪细胞向米色脂肪细 胞分化,建立棕色化成脂分化体系;利用 CCK-8 法检测不同浓度茯苓酸对 3T3-L1 MBX 存活率的影响;油红 O 染色观察茯苓酸作用后脂滴形成情况,并测定分化后 细胞中脂质含量;定量逆转录聚合酶链反应检测棕色化关键基因解耦联蛋白 (Ucp)1、过氧化物酶体增殖物激活受体 γ辅激活子(Pgc)-1α、含 PR结构域 16转录 因子(Prdm16),脂肪生成相关基因固醇调节元件结合蛋白(Srebp)1c、乙酰辅酶 A 羧化酶(Acc)、脂肪酸合成酶(Fas)以及脂肪分解相关基因激素敏感性脂肪酶 (Hsl)、脂肪甘油三酯水解酶(Atgl)、肉碱棕榈酰转移酶(Cpt)1 的表达情况。结果: 体外诱导 3T3-L1 MBX 成脂分化后呈米色脂肪细胞形态,高表达棕色化关键基因 Ucp1、Pgc-1α、Prdm16 和米色脂肪标记基因 Cd137、Tbx1、Tmem26。茯苓酸浓度在 0~80 μmol/L 时对 3T3-L1 MBX 均无细胞毒性;茯苓酸处理可显著抑制 3T3-L1 MBX 的分化,减少脂质积累含量(P<0.01),增加 Ucp1、Pgc-1α、Prdm16等棕色化关 键基因及其蛋白质表达,降低脂肪合成相关基因 Srebp1c、Acc、Fas 表达,增加脂肪 分解相关基因 Hsl、Atgl、Cpt1 表达(均 P<0.05),且 20 μmol/L 浓度处理效果相对更 明显。结论: 茯苓酸通过调控 3T3-L1 MBX 前脂肪细胞棕色化成脂分化抑制脂肪 合成,促进脂质分解。 [关键词] 茯苓酸;3T3-L1 MBX前脂肪细胞;脂肪细胞棕色化;成脂分化;脂代谢 [中图分类号] R285 [文献标志码] A Pachymic acid promotes brown/beige adipocyte differentiation and lipid metabolism in preadipocytes 3T3-L1 MBX DOI:10.3724/zdxbyxb-2024-0355 收稿日期(Received):2024-08-01 修改返回时间(Received in revised date):2024-11-26 接受日期(Accepted):2025-01-06 网络预发表日期(Online):2025-01-11 基金项目(Funding):国家自然科学基金(82374102);浙江中医药大学科研项目(2022JKZKTS21) 第一作者(First author): 陈坤铃 ,硕 士 研 究 生 ,主 要 从 事 中 医 药 调 节 脂 肪 代 谢 的 作 用 及 分 子 机 制 研 究 ;E-mail: chenkunling1997@163.com;https://orcid.org/0009-0007-6705-6622 通信作者(Corresponding author):周丽萍,实验师,主要从事中医药调节脂肪代谢的作用及分子机制研究;E-mail: lipingzhou@zcmu.edu.cn;https://orcid.org/0009-0006-1047-3954 · 专题报道 ·中医药现代化 Open Access 浙江大学学报(医学版) Journal of Zhejiang University(Medical Sciences) CHEN Kunling, DOU Xiaobing, LIN Yiyou, BAI Danyao, LUO Yangzhou, ZHOU Liping (School of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China) Corresponding author: ZHOU Liping; E-mail: lipingzhou@zcmu.edu.cn, https://orcid.org/ 0009-0006-1047-3954 [Abstract] Objective: To investigate the effect of pachymic acid on brown/beige adipocyte differentiation and lipid metabolism in preadipocytes 3T3-L1 MBX.. Methods: The brown cocktail method was employed to induce 3T3-L1 MBX cells to differentiate into beige adipocytes.. The impact of pachymic acid on the viability of 3T3-L1 MBX preadipocytes was evaluated using the CCK-8 assay. Polymerase Chain Reaction:Article Title: Pachymic acid promotes brown/beige adipocyte differentiation and lipid metabolism in preadipocytes 3T3-L1 MBX. Article Snippet: JOURNAL OF ZHEJIANG UNIVERSITY(MEDICAL SCIENCES) 浙 江 大 学 学 报( 医 学 版 ) XXXX 年 XX 月 XX XXXX 茯苓酸促进3T3-L1 MBX前脂肪细胞棕色 化成脂分化及脂代谢 陈坤铃,窦晓兵,林益游,白丹瑶,罗杨舟,周丽萍 浙江中医药大学生命科学学院,浙江 杭州 310053 [摘 要] 目的: 探究茯苓酸对 3T3-L1 MBX 前脂肪细胞棕色化成脂分化及脂代 谢的影响。方法: 采用棕色鸡尾酒法诱导 3T3-L1 MBX 前脂肪细胞向米色脂肪细 胞分化,建立棕色化成脂分化体系;利用 CCK-8 法检测不同浓度茯苓酸对 3T3-L1 MBX 存活率的影响;油红 O 染色观察茯苓酸作用后脂滴形成情况,并测定分化后 细胞中脂质含量;定量逆转录聚合酶链反应检测棕色化关键基因解耦联蛋白 (Ucp)1、过氧化物酶体增殖物激活受体 γ辅激活子(Pgc)-1α、含 PR结构域 16转录 因子(Prdm16),脂肪生成相关基因固醇调节元件结合蛋白(Srebp)1c、乙酰辅酶 A 羧化酶(Acc)、脂肪酸合成酶(Fas)以及脂肪分解相关基因激素敏感性脂肪酶 (Hsl)、脂肪甘油三酯水解酶(Atgl)、肉碱棕榈酰转移酶(Cpt)1 的表达情况。结果: 体外诱导 3T3-L1 MBX 成脂分化后呈米色脂肪细胞形态,高表达棕色化关键基因 Ucp1、Pgc-1α、Prdm16 和米色脂肪标记基因 Cd137、Tbx1、Tmem26。茯苓酸浓度在 0~80 μmol/L 时对 3T3-L1 MBX 均无细胞毒性;茯苓酸处理可显著抑制 3T3-L1 MBX 的分化,减少脂质积累含量(P<0.01),增加 Ucp1、Pgc-1α、Prdm16等棕色化关 键基因及其蛋白质表达,降低脂肪合成相关基因 Srebp1c、Acc、Fas 表达,增加脂肪 分解相关基因 Hsl、Atgl、Cpt1 表达(均 P<0.05),且 20 μmol/L 浓度处理效果相对更 明显。结论: 茯苓酸通过调控 3T3-L1 MBX 前脂肪细胞棕色化成脂分化抑制脂肪 合成,促进脂质分解。 [关键词] 茯苓酸;3T3-L1 MBX前脂肪细胞;脂肪细胞棕色化;成脂分化;脂代谢 [中图分类号] R285 [文献标志码] A Pachymic acid promotes brown/beige adipocyte differentiation and lipid metabolism in preadipocytes 3T3-L1 MBX DOI:10.3724/zdxbyxb-2024-0355 收稿日期(Received):2024-08-01 修改返回时间(Received in revised date):2024-11-26 接受日期(Accepted):2025-01-06 网络预发表日期(Online):2025-01-11 基金项目(Funding):国家自然科学基金(82374102);浙江中医药大学科研项目(2022JKZKTS21) 第一作者(First author): 陈坤铃 ,硕 士 研 究 生 ,主 要 从 事 中 医 药 调 节 脂 肪 代 谢 的 作 用 及 分 子 机 制 研 究 ;E-mail: chenkunling1997@163.com;https://orcid.org/0009-0007-6705-6622 通信作者(Corresponding author):周丽萍,实验师,主要从事中医药调节脂肪代谢的作用及分子机制研究;E-mail: lipingzhou@zcmu.edu.cn;https://orcid.org/0009-0006-1047-3954 · 专题报道 ·中医药现代化 Open Access 浙江大学学报(医学版) Journal of Zhejiang University(Medical Sciences) CHEN Kunling, DOU Xiaobing, LIN Yiyou, BAI Danyao, LUO Yangzhou, ZHOU Liping (School of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China) Corresponding author: ZHOU Liping; E-mail: lipingzhou@zcmu.edu.cn, https://orcid.org/ 0009-0006-1047-3954 [Abstract] Objective: To investigate the effect of pachymic acid on brown/beige adipocyte differentiation and lipid metabolism in preadipocytes 3T3-L1 MBX.. Methods: The brown cocktail method was employed to induce 3T3-L1 MBX cells to differentiate into beige adipocytes.. The impact of pachymic acid on the viability of 3T3-L1 MBX preadipocytes was evaluated using the CCK-8 assay. |