Journal: Neoplasma

Article Title: Decreased expression of Small glutamine-rich tetratricopeptide repeat-containing protein (SGT) correlated with prognosis of Hepatocellular carcinoma

doi: 10.4149/neo_2014_012

Figure Lengend Snippet: Figure 1. SGTB is low expressed in hepatocellular carcinoma but high in paired adjacent non-tumor liver tissues. Western blot of eight representa- tive paired samples of hepatocellular carcinoma tissue (T) and adjacent non-tumor liver tissues (N) immunoblotted against SGTB. Whole-cell lysates were prepared from tissue specimens obtained from hepatocellular carcinoma and adjacent non-tumor liver tissues. In all samples tested, SGTB expression was obviously lower in hepatocellular carcinoma than in paired adjacent non-tumor liver tissues. β-actin was used as a control for protein load and integrity.

Article Snippet: After that the sections were incubated overnight at 4 °C with anti-SGTB rabbit polyclonal antibody (diluted 1:100; Novus Biologicals), Negative control slides were also processed in parallel using a nonspecific immunoglobulin IgG (Sigma Chemical Co., St. Louis, MO) at the same concentration as the primary antibody.

Techniques: Western Blot, Expressing, Control

Journal: Neoplasma

Article Title: Decreased expression of Small glutamine-rich tetratricopeptide repeat-containing protein (SGT) correlated with prognosis of Hepatocellular carcinoma

doi: 10.4149/neo_2014_012

Figure Lengend Snippet: Figure 2. Immunohistochemical staining of SGTB in HCC tissues (a-f) and non-tumor liver tissues (g, h). Paraffin-embedded tissue sections were stained with antibodies for SGTB and counterstained with hematoxylin. (a and c) SGTB weak staining was shown in HCC tissues (200×), (b and d) with stain- ing predominant in the cytoplasm (400×). (e) SGTB strong staining was shown in HCC tissues (200×), (f) with staining predominant in the cytoplasm (400×). (g) SGTB immunoreactivity was identified in non-tumor liver tissues (200×), (h) with staining predominant in the cytoplasm (400×).

Article Snippet: After that the sections were incubated overnight at 4 °C with anti-SGTB rabbit polyclonal antibody (diluted 1:100; Novus Biologicals), Negative control slides were also processed in parallel using a nonspecific immunoglobulin IgG (Sigma Chemical Co., St. Louis, MO) at the same concentration as the primary antibody.

Techniques: Immunohistochemical staining, Staining

Journal: Neoplasma

Article Title: Decreased expression of Small glutamine-rich tetratricopeptide repeat-containing protein (SGT) correlated with prognosis of Hepatocellular carcinoma

doi: 10.4149/neo_2014_012

Figure Lengend Snippet: Figure 3. A Kaplan–Meier survival curves for low SGTB expression versus high SGTB expression in 90 patients of HCC showed a highly significant separation (P<0.001, logrank test). B Kaplan–Meier survival curves for low SGTB expression versus high SGTB expression in 74 male patients of HCC showed a highly significant separation (P<0.001, logrank test).

Article Snippet: After that the sections were incubated overnight at 4 °C with anti-SGTB rabbit polyclonal antibody (diluted 1:100; Novus Biologicals), Negative control slides were also processed in parallel using a nonspecific immunoglobulin IgG (Sigma Chemical Co., St. Louis, MO) at the same concentration as the primary antibody.

Techniques: Expressing