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mstrg 19853 1  (ATCC)


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    Structured Review

    ATCC mstrg 19853 1
    Mstrg 19853 1, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/19853/Escherichia+coli+(Migula)+Castellani+and+Chalmers/pm40597665-117-3-25
    Average 93 stars, based on 24 article reviews
    mstrg 19853 1 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Isolation:

    Article Title: An engineered IL-2 partial agonist promotes CD8 + T cell stemness
    Article Snippet: .. Mouse CD8 + T lymphocytes were isolated using the EasySep Mouse CD8 + T cell isolation kit (STEMCELL #19853) and cultured in RPMI-1640 medium (ATCC) supplemented with 10% FBS and 50 μM β-mercaptoethanol. ..

    Article Title: An engineered IL-2 partial agonist promotes CD8 + T cell stemness
    Article Snippet: .. Primary cell isolation, activation, culture, and retroviral transduction Mouse CD8 + T lymphocytes were isolated using the EasySep Mouse CD8 + T cell isolation kit (STEMCELL #19853) and cultured in RPMI-1640 medium (ATCC) supplemented with 10% FBS and 50 μM β-mercaptoethanol. ..

    Cell Isolation:

    Article Title: An engineered IL-2 partial agonist promotes CD8 + T cell stemness
    Article Snippet: .. Mouse CD8 + T lymphocytes were isolated using the EasySep Mouse CD8 + T cell isolation kit (STEMCELL #19853) and cultured in RPMI-1640 medium (ATCC) supplemented with 10% FBS and 50 μM β-mercaptoethanol. ..

    Article Title: An engineered IL-2 partial agonist promotes CD8 + T cell stemness
    Article Snippet: .. Primary cell isolation, activation, culture, and retroviral transduction Mouse CD8 + T lymphocytes were isolated using the EasySep Mouse CD8 + T cell isolation kit (STEMCELL #19853) and cultured in RPMI-1640 medium (ATCC) supplemented with 10% FBS and 50 μM β-mercaptoethanol. ..

    Cell Culture:

    Article Title: An engineered IL-2 partial agonist promotes CD8 + T cell stemness
    Article Snippet: .. Mouse CD8 + T lymphocytes were isolated using the EasySep Mouse CD8 + T cell isolation kit (STEMCELL #19853) and cultured in RPMI-1640 medium (ATCC) supplemented with 10% FBS and 50 μM β-mercaptoethanol. ..

    Article Title: An engineered IL-2 partial agonist promotes CD8 + T cell stemness
    Article Snippet: .. Primary cell isolation, activation, culture, and retroviral transduction Mouse CD8 + T lymphocytes were isolated using the EasySep Mouse CD8 + T cell isolation kit (STEMCELL #19853) and cultured in RPMI-1640 medium (ATCC) supplemented with 10% FBS and 50 μM β-mercaptoethanol. ..

    Activation Assay:

    Article Title: An engineered IL-2 partial agonist promotes CD8 + T cell stemness
    Article Snippet: .. Primary cell isolation, activation, culture, and retroviral transduction Mouse CD8 + T lymphocytes were isolated using the EasySep Mouse CD8 + T cell isolation kit (STEMCELL #19853) and cultured in RPMI-1640 medium (ATCC) supplemented with 10% FBS and 50 μM β-mercaptoethanol. ..

    Transduction:

    Article Title: An engineered IL-2 partial agonist promotes CD8 + T cell stemness
    Article Snippet: .. Primary cell isolation, activation, culture, and retroviral transduction Mouse CD8 + T lymphocytes were isolated using the EasySep Mouse CD8 + T cell isolation kit (STEMCELL #19853) and cultured in RPMI-1640 medium (ATCC) supplemented with 10% FBS and 50 μM β-mercaptoethanol. ..

    Reporter Assay:

    Article Title: Comprehensive transcriptome analysis reveals MSTRG.19853.1/ssc-miR-361-3p/ NPPA axis is related to hypoxic adaptation in Tibetan pigs
    Article Snippet: All primers were synthesized by BeiJing Genomics Institute (BGI, Beijing, China) (Table S ). .. The interaction between MSTRG.19853.1 and ssc-miR-361-3p, and between ssc-miR-361-3p and NPPA , was validated using a dual-luciferase reporter assay in chicken Dermal Fibroblast 1 (DF1) cells (ATCC, CRL-12203) due to their high transfection efficiency. .. The oligonucleotides used in this study, including miR-361-3p mimic and mimic negative control (NC), were synthesized by Gene Pharma Co., Ltd. (Shanghai, China) and are listed in Table S . Wild-type (WT) and mutant (Mut) sequences of MSTRG.19853.1 and NPPA 3’ UTR containing miR-361-3p binding sites were synthesized and cloned into the psi-CHECK TM −2 vector by Tsingke Biotechnology Co. Ltd (Beijing, China).

    Article Title: Comprehensive transcriptome analysis reveals MSTRG.19853.1/ssc-miR-361-3p/NPPA axis is related to hypoxic adaptation in Tibetan pigs.
    Article Snippet: All primers were synthesized by BeiJing Genomics Institute (BGI, Beijing, China) (Table S1). .. The interaction between MSTRG.19853.1 and ssc-miR361-3p, and between ssc-miR-361-3p and NPPA, was validated using a dual-luciferase reporter assay in chicken Dermal Fibroblast 1 (DF1) cells (ATCC, CRL-12203) due to their high transfection efficiency. ..

    Transfection:

    Article Title: Comprehensive transcriptome analysis reveals MSTRG.19853.1/ssc-miR-361-3p/ NPPA axis is related to hypoxic adaptation in Tibetan pigs
    Article Snippet: All primers were synthesized by BeiJing Genomics Institute (BGI, Beijing, China) (Table S ). .. The interaction between MSTRG.19853.1 and ssc-miR-361-3p, and between ssc-miR-361-3p and NPPA , was validated using a dual-luciferase reporter assay in chicken Dermal Fibroblast 1 (DF1) cells (ATCC, CRL-12203) due to their high transfection efficiency. .. The oligonucleotides used in this study, including miR-361-3p mimic and mimic negative control (NC), were synthesized by Gene Pharma Co., Ltd. (Shanghai, China) and are listed in Table S . Wild-type (WT) and mutant (Mut) sequences of MSTRG.19853.1 and NPPA 3’ UTR containing miR-361-3p binding sites were synthesized and cloned into the psi-CHECK TM −2 vector by Tsingke Biotechnology Co. Ltd (Beijing, China).

    Article Title: Comprehensive transcriptome analysis reveals MSTRG.19853.1/ssc-miR-361-3p/NPPA axis is related to hypoxic adaptation in Tibetan pigs.
    Article Snippet: All primers were synthesized by BeiJing Genomics Institute (BGI, Beijing, China) (Table S1). .. The interaction between MSTRG.19853.1 and ssc-miR361-3p, and between ssc-miR-361-3p and NPPA, was validated using a dual-luciferase reporter assay in chicken Dermal Fibroblast 1 (DF1) cells (ATCC, CRL-12203) due to their high transfection efficiency. ..

    other:

    Article Title: A Comparison of Porphyrin Photosensitizers in Photodynamic Inactivation of RNA and DNA Bacteriophages
    Article Snippet: fr (ATCC 15767-B1) , E. coli (ATCC 19853) , IV ((+)ssRNA) , 19–23 , 3.5 [ ] .



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