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Image Search Results
Journal: The Journal of Clinical Investigation
Article Title: Protein arginine methyltransferase 5 promotes cholesterol biosynthesis–mediated Th17 responses and autoimmunity
doi: 10.1172/JCI131254
Figure Lengend Snippet: (A) Schematic of Prmt5 long and short isoform transcripts and Prmt5 genomic locus targeting strategy. Exon 7, an exon common to all protein-coding Prmt5 isoforms, was flanked by loxP sites to provide deletion of both isoforms in Cre-expressing cells. (B) Schematic of expected PRMT5 deletion in thymus T cell precursors and peripheral T cells in 2 different transgenic models, namely T-PRMT5Δ/Δ (constitutive) and iCD4-PRMT5Δ/Δ (inducible). (C and D) PCR amplification of genomic DNA isolated from CD4+ and CD8+ T cells from (C) T-PRMT5Δ/Δ or (D) iCD4-PRMT5Δ/Δ mice, as well as control PRMT5fl/fl mice. PRMT5-KO band: 283 bp; full-length floxed PRMT5 band: 1100 bp. DN, double negative; DP, double positive; SP, single positive.
Article Snippet: CD3 + (Stem Cell Technologies, catalog no. 19851),
Techniques: Expressing, Transgenic Assay, Amplification, Isolation, Control
Journal: The Journal of Clinical Investigation
Article Title: Protein arginine methyltransferase 5 promotes cholesterol biosynthesis–mediated Th17 responses and autoimmunity
doi: 10.1172/JCI131254
Figure Lengend Snippet: (A) Gating strategy for analysis of thymocyte populations and representative thymic Treg percentage plots. Live/dead gating was also performed after FSC/SSC but is not displayed. (B–G) Thymocytes were analyzed by flow cytometry and (B) B220–, (C) DN, (D) DP, (E) CD4 SP, (F) Treg, and (G) CD8 SP thymocyte numbers were calculated. (H–N) Splenocytes were analyzed by flow cytometry (gating strategy in I and H) B220– splenocyte, (J) CD4+, (K) Treg, (L) CD8+, (M) CD4+ Tem, Tcm, and naive populations, and (N) CD8+ Tem, Tcm, and naive populations were calculated. (O) iNK T cell flow cytometric analysis gating strategy and total (P) thymic and (Q) splenic iNKT cell numbers. Data are representative of 4 independent experiments. Shown n = 4–5 independent mice of matched age. One-way ANOVA, followed by Sidak’s (B–H and J–N) or Tukey’s (P and Q) multiple-comparisons test: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Bar graphs display mean ± SD. DN, double negative; DP, double positive; SP, single positive; Tem, effector memory T cells; Tcm, central memory T cells.
Article Snippet: CD3 + (Stem Cell Technologies, catalog no. 19851),
Techniques: Flow Cytometry