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incubation  (Proteintech)


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  • 93

    Structured Review

    Proteintech incubation
    Incubation, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/14693/NKRF+Antibody/pm41554300-165-10-19
    Average 93 stars, based on 10 article reviews
    incubation - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells
    Article Snippet: .. After three PBS washes, cells were blocked with 0.5% BSA in PBS, and incubated with the anti-SLC25A39 (Proteintech, 14693–1-AP, 1:200) and anti-ATP5A (Thermo Fisher Scientific, 439800, 1:200) primary antibodies in blocking solution for 1 h. Cells were washed 3 times with PBS and incubated with a donkey anti-mouse Alexa Fluor 568 (ThermoFisher Scientific, A10037) and donkey anti-rabbit Alexa Fluor 488 (ThermoFisher Scientific, A21206) secondary antibodies (1:400) for 1 h in the dark. ..

    Article Title: E3 Ubiquitination Ligase MYLIP Mediates the NKRF/SLC25A34 Axis to Suppress Malignant Progression in Colorectal Cancer.
    Article Snippet: Background The SLC25 family of transport proteins has been implicated in colorectal cancer (CRC) tumorigenesis.. However, the potential role of SLC25A34 in CRC remains unclear.. This study was designed to elucidate the role and mechanism of SLC25A34 in CRC.

    Article Title: Treadmill Exercise Attenuates CUMS-Induced Depressive Behaviors by Modulating the UPRmt via the Nrf2/Keap1 Pathway.
    Article Snippet: .. Following a 1-hour block at room temperature, sections were incubated overnight (4 °C) with primary antibodies against Nrf2 (14693-1-AP, 1:200, Proteintech) or HSP60 (ab190828, 1:200, Abcam). ..

    Article Title: Treadmill Exercise Attenuates CUMS-Induced Depressive Behaviors by Modulating the UPRmt via the Nrf2/Keap1 Pathway.
    Article Snippet: .. The membranes were then blocked (1 h, RT) before overnight incubation (4 °C) with primary antibodies: Nrf2 (14693-1-AP, 1:1,000, Proteintech), Keap1 (10503-2-AP, 1:1,000, Proteintech), NQO1 (ab80588, 1:1,000, Abcam), HO-1 (ab189491, 1:1,000, Abcam), HSP60 (ab190828, 1:1,000, Abcam), ClpP (ab124822, 1:1,000, Abcam), HSP70 (ab194360, 1:1,500, Abcam), LONP1 (15440-1-AP, 1:1,000, Proteintech), ATF5 (MA563234, 1:1,000, Thermo Fisher), CHOP (15204-1-AP, 1:1,000, Proteintech), and β-actin (ab179467, 1:5,000, Abcam). .. Subsequently, membranes were incubated with an HRP-conjugated secondary antibody (ab6721, 1:10,000, Abcam) for 1 h at RT, washed (3 × 10 min, TBST), and visualized via an ECL system.

    Blocking Assay:

    Article Title: SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells
    Article Snippet: .. After three PBS washes, cells were blocked with 0.5% BSA in PBS, and incubated with the anti-SLC25A39 (Proteintech, 14693–1-AP, 1:200) and anti-ATP5A (Thermo Fisher Scientific, 439800, 1:200) primary antibodies in blocking solution for 1 h. Cells were washed 3 times with PBS and incubated with a donkey anti-mouse Alexa Fluor 568 (ThermoFisher Scientific, A10037) and donkey anti-rabbit Alexa Fluor 488 (ThermoFisher Scientific, A21206) secondary antibodies (1:400) for 1 h in the dark. ..

    Article Title: Treadmill Exercise Attenuates CUMS-Induced Depressive Behaviors by Modulating the UPRmt via the Nrf2/Keap1 Pathway.
    Article Snippet: .. Following a 1-hour block at room temperature, sections were incubated overnight (4 °C) with primary antibodies against Nrf2 (14693-1-AP, 1:200, Proteintech) or HSP60 (ab190828, 1:200, Abcam). ..

    Western Blot:

    Article Title: Silencing of lncRNA Gm26917 Attenuates Alveolar Macrophage-mediated Inflammatory Response in LPS-induced Acute Lung Injury Via Inhibiting NKRF Ubiquitination.
    Article Snippet: .. The antibodies used for Western blotting, co-immunoprecipitation or immunofluorescence are as follows: NKRF (DF8755,Affinity), GAPDH (T004,Affinity), His-Tag antibody (66,005–1-Ig, Proteintech), Flag-Tag antibody (66,008–4-Ig, Proteintech), IgG(A7028,Beyotime), p65 (YM3111,Immunoway), p-p65 (YP0191, Immunoway), IKBα (10,268–1-AP, Proteintech), p-IKBα (82,349–1- RR, Proteintech), p50 (66,992–1-Ig, Proteintech), F4 / 80 (sc377009,SantaCruz), CD68 (Ab125212,Abcam). .. Data were analyzed using GraphPad Prism 8.0.2 software.

    Article Title: Silencing of lncRNA Gm26917 Attenuates Alveolar Macrophage-mediated Inflammatory Response in LPS-induced Acute Lung Injury Via Inhibiting NKRF Ubiquitination
    Article Snippet: .. The antibodies used for Western blotting, co-immunoprecipitation or immunofluorescence are as follows: NKRF (DF8755,Affinity), GAPDH (T004,Affinity), His-Tag antibody (66,005–1-Ig, Proteintech), Flag-Tag antibody (66,008–4-Ig, Proteintech), IgG(A7028,Beyotime), p65 (YM3111,Immunoway), p-p65 (YP0191, Immunoway), IKBα (10,268–1-AP, Proteintech), p-IKBα (82,349–1-RR, Proteintech), p50 (66,992–1-Ig, Proteintech), F4 / 80 (sc377009,SantaCruz), CD68 ( Ab125212 ,Abcam). .. Data were analyzed using GraphPad Prism 8.0.2 software.

    Immunofluorescence:

    Article Title: Silencing of lncRNA Gm26917 Attenuates Alveolar Macrophage-mediated Inflammatory Response in LPS-induced Acute Lung Injury Via Inhibiting NKRF Ubiquitination.
    Article Snippet: .. The antibodies used for Western blotting, co-immunoprecipitation or immunofluorescence are as follows: NKRF (DF8755,Affinity), GAPDH (T004,Affinity), His-Tag antibody (66,005–1-Ig, Proteintech), Flag-Tag antibody (66,008–4-Ig, Proteintech), IgG(A7028,Beyotime), p65 (YM3111,Immunoway), p-p65 (YP0191, Immunoway), IKBα (10,268–1-AP, Proteintech), p-IKBα (82,349–1- RR, Proteintech), p50 (66,992–1-Ig, Proteintech), F4 / 80 (sc377009,SantaCruz), CD68 (Ab125212,Abcam). .. Data were analyzed using GraphPad Prism 8.0.2 software.

    Article Title: Silencing of lncRNA Gm26917 Attenuates Alveolar Macrophage-mediated Inflammatory Response in LPS-induced Acute Lung Injury Via Inhibiting NKRF Ubiquitination
    Article Snippet: .. The antibodies used for Western blotting, co-immunoprecipitation or immunofluorescence are as follows: NKRF (DF8755,Affinity), GAPDH (T004,Affinity), His-Tag antibody (66,005–1-Ig, Proteintech), Flag-Tag antibody (66,008–4-Ig, Proteintech), IgG(A7028,Beyotime), p65 (YM3111,Immunoway), p-p65 (YP0191, Immunoway), IKBα (10,268–1-AP, Proteintech), p-IKBα (82,349–1-RR, Proteintech), p50 (66,992–1-Ig, Proteintech), F4 / 80 (sc377009,SantaCruz), CD68 ( Ab125212 ,Abcam). .. Data were analyzed using GraphPad Prism 8.0.2 software.

    Control:

    Article Title: E3 Ubiquitination Ligase MYLIP Mediates the NKRF/SLC25A34 Axis to Suppress Malignant Progression in Colorectal Cancer.
    Article Snippet: Background The SLC25 family of transport proteins has been implicated in colorectal cancer (CRC) tumorigenesis.. However, the potential role of SLC25A34 in CRC remains unclear.. This study was designed to elucidate the role and mechanism of SLC25A34 in CRC.

    Lysis:

    Article Title: E3 Ubiquitination Ligase MYLIP Mediates the NKRF/SLC25A34 Axis to Suppress Malignant Progression in Colorectal Cancer.
    Article Snippet: Background The SLC25 family of transport proteins has been implicated in colorectal cancer (CRC) tumorigenesis.. However, the potential role of SLC25A34 in CRC remains unclear.. This study was designed to elucidate the role and mechanism of SLC25A34 in CRC.



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