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Image Search Results
Journal: Nature communications
Article Title: Androgens contribute to sex bias of autoimmunity in mice by T cell-intrinsic regulation of Ptpn22 phosphatase expression.
doi: 10.1038/s41467-024-51869-7
Figure Lengend Snippet: Fig. 8 | Disruption of the ARE motif in the 5’UTR of Ptpn22 modulates its expression in male T cells. male T cell activity and T cell functional popula- tions. a RT-PCR quantification of Ptpn22 transcript levels in DN (left) and CD8SP (right) thymocytes from 8-week-old NOD males that were either sham-operated (filled circles) or castrated (open circles) as 4-week-olds. Mean ± sem. Significance was cal- culated using an unpaired t-test. Symbols - individual mice. n = 4/group. b RT-PCR quantification of Ptpn22 in DN (left) and CD8SP (right) thymocytes from 8 to 9-week- old NOD AR wild-type (filles circles) and knock-out (open circles) males. Delta CT inversely correlates with the strength of expression. Symbols – individual mice. Combined data from two experiments. Number of mice: AR wild-type n = 12/group, AR KO n = 7/group. Mean ± sem. Significance was calculated using an unpaired t-test. The significance of interaction between sex and Ptpn22 mutation was calculated using
Article Snippet: Following list of primary antibodies was used:
Techniques: Disruption, Expressing, Activity Assay, Functional Assay, Reverse Transcription Polymerase Chain Reaction, Knock-Out, Mutagenesis