trap buffer (0.1 m acetate buffer: 0.2 m sodium acetate solution, 0.2 m acetic acid) (Millipore)
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trap buffer (0.1 m acetate buffer: 0.2 m sodium acetate solution, 0.2 m acetic acid)
Trap Buffer (0.1 M Acetate Buffer: 0.2 M Sodium Acetate Solution, 0.2 M Acetic Acid), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trap+buffer+solution/trap+buffer/pmc09116137-88-5-26
Average 90 stars, based on 1 article reviews
Trap Buffer (0.1 M Acetate Buffer: 0.2 M Sodium Acetate Solution, 0.2 M Acetic Acid), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trap+buffer+solution/trap+buffer/pmc09116137-88-5-26
Average 90 stars, based on 1 article reviews
trap buffer (0.1 m acetate buffer: 0.2 m sodium acetate solution, 0.2 m acetic acid) - by Bioz Stars,
2026-10
90/100 stars
Images
1) Product Images from "Modulation of Differentiation and Bone Resorbing Activity of Human (Pre-) Osteoclasts After X-Ray Exposure"
Article Title: Modulation of Differentiation and Bone Resorbing Activity of Human (Pre-) Osteoclasts After X-Ray Exposure
Journal: Frontiers in Immunology
doi: 10.3389/fimmu.2022.817281
Figure Legend Snippet: Identification of mature osteoclasts (mOC) on bone slices and quantification of resorbing activity 21 days after onset of the differentiation process. A triple staining for OC markers was applied (TRAP, red; F-actin, green; nuclei: blue; (A) . A cell was classified as mOC when it TRAP-positive and had ≥ 3 nuclei (white arrowheads; all mOC showed an actin ring, (B) , precursor (preOC, round with one or two nuclei, yellow arrowheads, (C) , proOC (spindle-shaped with a single nucleus, red arrowhead, (D) . After microscopy, cells were removed, and resorption pits were visualized by Toluidine blue staining (E) . Semi-quantitative image analysis was performed using ImageJ and masks to measure the resorbed areas per field of view (F) . Scale bar: 50 µm (A-D) , 100 µm (E, F) .
Techniques Used: Activity Assay, Staining, Microscopy
Related Articles
other:Article Title: The chromatin remodeling factor Arid1a cooperates with Jun/Fos to promote osteoclastogenesis by epigenetically upregulating Siglec15 expression. Article Snippet: Specifically, paraffin sections were stained with TRAP working Article Title: SENP3 Suppresses Osteoclastogenesis by De-conjugating SUMO2/3 from IRF8 in Bone Marrow-Derived Monocytes. Article Snippet: Paraffin sections were stained in TRAP working Article Title: The role of miR-150 regulates bone cell differentiation and function. Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Staining:Article Title: Modulation of Differentiation and Bone Resorbing Activity of Human (Pre-) Osteoclasts After X-Ray Exposure Article Snippet: After fixation with 3.7 % PFA (Merck KGaA, Darmstadt, Germany) for 15 min at RT cells were incubated with TRAP staining solution for 10 min at 37°C. .. The staining solution consists of Activity Assay:Article Title: Anti-osteoclastogenic diacetylenic components of Dendropanax morbifera. Article Snippet: Methanol (MeOH) extract of the aerial parts of Dendropanax morbifera (Araliaceae) has demonstrated a significant dose-dependent inhibitory effect on the RANKL-induced differentiation of bone marrow-derived macrophages to osteoclasts.. Bioassay-guided fractionation of the extract resulted in the isolation of a novel diacetylene carboxylic acid (1), together with a known diacetylenic compounds (2) as phytochemicals to strongly inhibit the osteoclast differentiation.. The chemical structure of 1 was determined by spectroscopic analyses as (9Z,16S)-16O-acetyl-9,17-octadecadiene-12,14-diynoic acid, that is acetyl derivative of 2. |