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trap solution buffer  (Merck & Co)

 
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    Merck & Co trap solution buffer
    Trap Solution Buffer, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap+buffer+solution/buffer+solution/pmc12445851-53-46-52
    Average 86 stars, based on 1 article reviews
    trap solution buffer - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Incubation:

    Article Title: G protein-coupled receptor 35 stimulation reduces osteoclast activity in primary human bone cells
    Article Snippet: Sample images were taken using Olympus cellSens software (Olympus). .. On day 10 of differentiation, all cells were collected using Accutase and cell scraping, then seeded in 96-well plates and exposed to agonists and/or antagonists for 72 h. Conditioned media was collected and transferred in 10 μL duplicates into a 96-well clear plate, then 90 μL TRAP solution buffer (1 M acetate (Merck), 0.5% Triton X-100 (Sigma-Aldrich), 1 M NaCl (Sigma-Aldrich), 10 mM EDTA (Fisher Scientific), 50 mM L-Ascorbic acid (Merck), 0.2 M disodium tartrate (Sigma-Aldrich), 82 mM 4-nitrolphenylphosphate (Sigma-Aldrich)) added to each well, prior to incubation in the dark for 30 min at 37 °C. .. The reaction was stopped by adding 0.3 M NaOH (Honeywell, SLS, Hessle, United Kingdom) and absorbance was measured at 405 nm on a SpectraMax ABS (Molecular Devices) or GloMax plate reader.

    Nuclear Magnetic Resonance:

    Article Title: 1 H nuclear magnetic resonance spectroscopy metabolomics of giraffe milk during mid- to late-lactation.
    Article Snippet: The end of lactation, also known as involution or the dryout period, is the transition from the lactating to the nonlactating state.. The transition may be gradual, with offspring naturally weaned over time, or it may be sudden, with milk removal abruptly ceasing.. The latter is practised in livestock management (Hurley, 1989).

    other:

    Article Title: Quenching-Chemiluminescence New Method for Determination of Ethylmorphine by New Design Chemiluminescence Flow Injection System
    Article Snippet: 1- 0.1M Na2 CO3 (Merck) buffer solution: was prepared by dissolving 10.50 g of Na2 CO3 and 0.5M NaOH(20 g) to exactly 1liter (16) 2- 0.02 M Luminol solution: (5-amino-2,3-dihydro-1,4_phthalazinedione) (Sigma-Aldrich , St. Louis, MO, USA) was prepared by dissolving 0.3544 g of luminol (97%) in 100 ml of 0.1M Na2 CO3 solution the buffer(pH 10) (17).



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    Identification of mature osteoclasts (mOC) on bone slices and quantification of resorbing activity 21 days after onset of the differentiation process. A <t>triple</t> <t>staining</t> for OC markers was applied <t>(TRAP,</t> red; F-actin, green; nuclei: blue; (A) . A cell was classified as mOC when it TRAP-positive and had ≥ 3 nuclei (white arrowheads; all mOC showed an actin ring, (B) , precursor (preOC, round with one or two nuclei, yellow arrowheads, (C) , proOC (spindle-shaped with a single nucleus, red arrowhead, (D) . After microscopy, cells were removed, and resorption pits were visualized by Toluidine blue staining (E) . Semi-quantitative image analysis was performed using ImageJ and masks to measure the resorbed areas per field of view (F) . Scale bar: 50 µm (A-D) , 100 µm (E, F) .
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    Identification of mature osteoclasts (mOC) on bone slices and quantification of resorbing activity 21 days after onset of the differentiation process. A <t>triple</t> <t>staining</t> for OC markers was applied <t>(TRAP,</t> red; F-actin, green; nuclei: blue; (A) . A cell was classified as mOC when it TRAP-positive and had ≥ 3 nuclei (white arrowheads; all mOC showed an actin ring, (B) , precursor (preOC, round with one or two nuclei, yellow arrowheads, (C) , proOC (spindle-shaped with a single nucleus, red arrowhead, (D) . After microscopy, cells were removed, and resorption pits were visualized by Toluidine blue staining (E) . Semi-quantitative image analysis was performed using ImageJ and masks to measure the resorbed areas per field of view (F) . Scale bar: 50 µm (A-D) , 100 µm (E, F) .
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    Image Search Results


    Identification of mature osteoclasts (mOC) on bone slices and quantification of resorbing activity 21 days after onset of the differentiation process. A triple staining for OC markers was applied (TRAP, red; F-actin, green; nuclei: blue; (A) . A cell was classified as mOC when it TRAP-positive and had ≥ 3 nuclei (white arrowheads; all mOC showed an actin ring, (B) , precursor (preOC, round with one or two nuclei, yellow arrowheads, (C) , proOC (spindle-shaped with a single nucleus, red arrowhead, (D) . After microscopy, cells were removed, and resorption pits were visualized by Toluidine blue staining (E) . Semi-quantitative image analysis was performed using ImageJ and masks to measure the resorbed areas per field of view (F) . Scale bar: 50 µm (A-D) , 100 µm (E, F) .

    Journal: Frontiers in Immunology

    Article Title: Modulation of Differentiation and Bone Resorbing Activity of Human (Pre-) Osteoclasts After X-Ray Exposure

    doi: 10.3389/fimmu.2022.817281

    Figure Lengend Snippet: Identification of mature osteoclasts (mOC) on bone slices and quantification of resorbing activity 21 days after onset of the differentiation process. A triple staining for OC markers was applied (TRAP, red; F-actin, green; nuclei: blue; (A) . A cell was classified as mOC when it TRAP-positive and had ≥ 3 nuclei (white arrowheads; all mOC showed an actin ring, (B) , precursor (preOC, round with one or two nuclei, yellow arrowheads, (C) , proOC (spindle-shaped with a single nucleus, red arrowhead, (D) . After microscopy, cells were removed, and resorption pits were visualized by Toluidine blue staining (E) . Semi-quantitative image analysis was performed using ImageJ and masks to measure the resorbed areas per field of view (F) . Scale bar: 50 µm (A-D) , 100 µm (E, F) .

    Article Snippet: The staining solution consists of TRAP buffer (0.1 M acetate buffer: 35.2 ml 0.2 M Sodium Acetate solution, 14.8 ml 0.2 M acetic acid, 50 ml; Millipore, Burlington, USA) mixed with 0.3 M Sodium Tartrate (pH 5.5), 10 mg/ml Naphthol AS-MX phosphate, Triton X-100, 0.3 mg/ml Fast Red Violet LB Salt (all from Sigma Aldrich Chemie GmbH).

    Techniques: Activity Assay, Staining, Microscopy