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tartrate-resistant acid phosphatase (trap) buffer solution  (FUJIFILM)

 
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    Structured Review

    FUJIFILM tartrate-resistant acid phosphatase (trap) buffer solution
    Tartrate Resistant Acid Phosphatase (Trap) Buffer Solution, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap+buffer+solution/tartrate+resistant+acid+phosphatase++trap++staining+kit/pm35584937-84-0-19
    Average 90 stars, based on 1 article reviews
    tartrate-resistant acid phosphatase (trap) buffer solution - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Staining:

    Article Title: Zymosan-Treated SKG Mice: Assessing Effects of Systemic Inflammation on the Temporomandibular Joint.
    Article Snippet: Objectives: The effects of systemic inflammation on the temporomandibular joint (TMJ) are poorly understood.. This study aimed to establish a mouse model to study the effects of systemic inflammation on the TMJ.. Materials and Methods: SKG mice, a BALB/c strain with spontaneous onset of rheumatoid arthritislike symptoms due to a spontaneous point mutation (W163C) in the gene encoding the SH2 domain of ZAP70, were treated with zymosan (β1,3glucan).

    Article Title: Therapeutic Potential of Targeting Ferroptosis in Periprosthetic Osteolysis Induced by Ultra-High-Molecular-Weight Polyethylene Wear Debris
    Article Snippet: The paraffin blocks were sectionized and stained with hematoxylin and eosin and tartrate resistance acid phosphatase (TRAP) staining (Wako, Japan).

    Article Title: Pim1 promotes the maintenance of bone homeostasis by regulating osteoclast function.
    Article Snippet: The tartrate-resistant phosphatase (TRAP) staining kit was purchased from FUJIFILM Wako Pure Chemical Corporation.

    Article Title: Xenogenous implanted dental follicle stem cells promote periodontal regeneration through inducing the N2 phenotype of neutrophils
    Article Snippet: Staining procedures, including Hematoxylin-Eosin (H&E, Solarbio, China), Masson’s trichrome (Solarbio), Tartrate-Resistant Acid Phosphatase (TRAP, Wako, Japan), immunohistochemistry (IHC), and immunofluorescence (IF), were performed following previously established methods [ ].

    Article Title: Rheumatoid arthritis severity is mediated by crosstalk between synoviocytes and mature osteoclasts through a calcium and cytokine feedback loop.
    Article Snippet: Thereafter, the fixed cells and tissues were stained with a tartrate-resistant acid phosphatase (TRAP)/alkaline phosphatase staining kit (FUJIFILM) according to the manufacturer’s instructions.

    Article Title: FOXO1-mTOR pathway in vascular pericyte regulates the formation of type H vessels to control bone metabolism
    Article Snippet: The activity of osteoclasts was detected by tartrate-resistant acid phosphatase (TRAP) staining kits (Cat. No. 294–67001, Wako, Japan) following the manufacturers’ instructions.

    Article Title: Mitochondria-targeted delivery of zinc-coordinated resveratrol nanoparticles rescues the osteogenic potential of periodontal ligament stem cells compromised by inflammation for periodontal wound healing
    Article Snippet: In addition, a tartrate-resistant acid phosphatase (TRAP) staining kit (294–67001; Wako, Japan) was used to examine bone resorption activity.

    Control:

    Article Title: Zymosan-Treated SKG Mice: Assessing Effects of Systemic Inflammation on the Temporomandibular Joint.
    Article Snippet: Objectives: The effects of systemic inflammation on the temporomandibular joint (TMJ) are poorly understood.. This study aimed to establish a mouse model to study the effects of systemic inflammation on the TMJ.. Materials and Methods: SKG mice, a BALB/c strain with spontaneous onset of rheumatoid arthritislike symptoms due to a spontaneous point mutation (W163C) in the gene encoding the SH2 domain of ZAP70, were treated with zymosan (β1,3glucan).

    Article Title: Therapeutic Potential of Targeting Ferroptosis in Periprosthetic Osteolysis Induced by Ultra-High-Molecular-Weight Polyethylene Wear Debris
    Article Snippet: The paraffin blocks were sectionized and stained with hematoxylin and eosin and tartrate resistance acid phosphatase (TRAP) staining (Wako, Japan).

    Article Title: Pim1 promotes the maintenance of bone homeostasis by regulating osteoclast function.
    Article Snippet: The tartrate-resistant phosphatase (TRAP) staining kit was purchased from FUJIFILM Wako Pure Chemical Corporation.

    Article Title: Xenogenous implanted dental follicle stem cells promote periodontal regeneration through inducing the N2 phenotype of neutrophils
    Article Snippet: Staining procedures, including Hematoxylin-Eosin (H&E, Solarbio, China), Masson’s trichrome (Solarbio), Tartrate-Resistant Acid Phosphatase (TRAP, Wako, Japan), immunohistochemistry (IHC), and immunofluorescence (IF), were performed following previously established methods [ ].

    Article Title: Rheumatoid arthritis severity is mediated by crosstalk between synoviocytes and mature osteoclasts through a calcium and cytokine feedback loop.
    Article Snippet: Thereafter, the fixed cells and tissues were stained with a tartrate-resistant acid phosphatase (TRAP)/alkaline phosphatase staining kit (FUJIFILM) according to the manufacturer’s instructions.

    Article Title: FOXO1-mTOR pathway in vascular pericyte regulates the formation of type H vessels to control bone metabolism
    Article Snippet: The activity of osteoclasts was detected by tartrate-resistant acid phosphatase (TRAP) staining kits (Cat. No. 294–67001, Wako, Japan) following the manufacturers’ instructions.

    Article Title: Mitochondria-targeted delivery of zinc-coordinated resveratrol nanoparticles rescues the osteogenic potential of periodontal ligament stem cells compromised by inflammation for periodontal wound healing
    Article Snippet: In addition, a tartrate-resistant acid phosphatase (TRAP) staining kit (294–67001; Wako, Japan) was used to examine bone resorption activity.

    Comparison:

    Article Title: Zymosan-Treated SKG Mice: Assessing Effects of Systemic Inflammation on the Temporomandibular Joint.
    Article Snippet: Objectives: The effects of systemic inflammation on the temporomandibular joint (TMJ) are poorly understood.. This study aimed to establish a mouse model to study the effects of systemic inflammation on the TMJ.. Materials and Methods: SKG mice, a BALB/c strain with spontaneous onset of rheumatoid arthritislike symptoms due to a spontaneous point mutation (W163C) in the gene encoding the SH2 domain of ZAP70, were treated with zymosan (β1,3glucan).

    Article Title: Therapeutic Potential of Targeting Ferroptosis in Periprosthetic Osteolysis Induced by Ultra-High-Molecular-Weight Polyethylene Wear Debris
    Article Snippet: The paraffin blocks were sectionized and stained with hematoxylin and eosin and tartrate resistance acid phosphatase (TRAP) staining (Wako, Japan).

    Article Title: Pim1 promotes the maintenance of bone homeostasis by regulating osteoclast function.
    Article Snippet: The tartrate-resistant phosphatase (TRAP) staining kit was purchased from FUJIFILM Wako Pure Chemical Corporation.

    Article Title: Xenogenous implanted dental follicle stem cells promote periodontal regeneration through inducing the N2 phenotype of neutrophils
    Article Snippet: Staining procedures, including Hematoxylin-Eosin (H&E, Solarbio, China), Masson’s trichrome (Solarbio), Tartrate-Resistant Acid Phosphatase (TRAP, Wako, Japan), immunohistochemistry (IHC), and immunofluorescence (IF), were performed following previously established methods [ ].

    Article Title: Rheumatoid arthritis severity is mediated by crosstalk between synoviocytes and mature osteoclasts through a calcium and cytokine feedback loop.
    Article Snippet: Thereafter, the fixed cells and tissues were stained with a tartrate-resistant acid phosphatase (TRAP)/alkaline phosphatase staining kit (FUJIFILM) according to the manufacturer’s instructions.

    Article Title: FOXO1-mTOR pathway in vascular pericyte regulates the formation of type H vessels to control bone metabolism
    Article Snippet: The activity of osteoclasts was detected by tartrate-resistant acid phosphatase (TRAP) staining kits (Cat. No. 294–67001, Wako, Japan) following the manufacturers’ instructions.

    Article Title: Mitochondria-targeted delivery of zinc-coordinated resveratrol nanoparticles rescues the osteogenic potential of periodontal ligament stem cells compromised by inflammation for periodontal wound healing
    Article Snippet: In addition, a tartrate-resistant acid phosphatase (TRAP) staining kit (294–67001; Wako, Japan) was used to examine bone resorption activity.

    Two Tailed Test:

    Article Title: Zymosan-Treated SKG Mice: Assessing Effects of Systemic Inflammation on the Temporomandibular Joint.
    Article Snippet: Objectives: The effects of systemic inflammation on the temporomandibular joint (TMJ) are poorly understood.. This study aimed to establish a mouse model to study the effects of systemic inflammation on the TMJ.. Materials and Methods: SKG mice, a BALB/c strain with spontaneous onset of rheumatoid arthritislike symptoms due to a spontaneous point mutation (W163C) in the gene encoding the SH2 domain of ZAP70, were treated with zymosan (β1,3glucan).

    Article Title: Therapeutic Potential of Targeting Ferroptosis in Periprosthetic Osteolysis Induced by Ultra-High-Molecular-Weight Polyethylene Wear Debris
    Article Snippet: The paraffin blocks were sectionized and stained with hematoxylin and eosin and tartrate resistance acid phosphatase (TRAP) staining (Wako, Japan).

    Article Title: Pim1 promotes the maintenance of bone homeostasis by regulating osteoclast function.
    Article Snippet: The tartrate-resistant phosphatase (TRAP) staining kit was purchased from FUJIFILM Wako Pure Chemical Corporation.

    Article Title: Xenogenous implanted dental follicle stem cells promote periodontal regeneration through inducing the N2 phenotype of neutrophils
    Article Snippet: Staining procedures, including Hematoxylin-Eosin (H&E, Solarbio, China), Masson’s trichrome (Solarbio), Tartrate-Resistant Acid Phosphatase (TRAP, Wako, Japan), immunohistochemistry (IHC), and immunofluorescence (IF), were performed following previously established methods [ ].

    Article Title: Rheumatoid arthritis severity is mediated by crosstalk between synoviocytes and mature osteoclasts through a calcium and cytokine feedback loop.
    Article Snippet: Thereafter, the fixed cells and tissues were stained with a tartrate-resistant acid phosphatase (TRAP)/alkaline phosphatase staining kit (FUJIFILM) according to the manufacturer’s instructions.

    Article Title: FOXO1-mTOR pathway in vascular pericyte regulates the formation of type H vessels to control bone metabolism
    Article Snippet: The activity of osteoclasts was detected by tartrate-resistant acid phosphatase (TRAP) staining kits (Cat. No. 294–67001, Wako, Japan) following the manufacturers’ instructions.

    Article Title: Mitochondria-targeted delivery of zinc-coordinated resveratrol nanoparticles rescues the osteogenic potential of periodontal ligament stem cells compromised by inflammation for periodontal wound healing
    Article Snippet: In addition, a tartrate-resistant acid phosphatase (TRAP) staining kit (294–67001; Wako, Japan) was used to examine bone resorption activity.

    Activity Assay:

    Article Title: Zymosan-Treated SKG Mice: Assessing Effects of Systemic Inflammation on the Temporomandibular Joint.
    Article Snippet: Objectives: The effects of systemic inflammation on the temporomandibular joint (TMJ) are poorly understood.. This study aimed to establish a mouse model to study the effects of systemic inflammation on the TMJ.. Materials and Methods: SKG mice, a BALB/c strain with spontaneous onset of rheumatoid arthritislike symptoms due to a spontaneous point mutation (W163C) in the gene encoding the SH2 domain of ZAP70, were treated with zymosan (β1,3glucan).

    Article Title: Therapeutic Potential of Targeting Ferroptosis in Periprosthetic Osteolysis Induced by Ultra-High-Molecular-Weight Polyethylene Wear Debris
    Article Snippet: The paraffin blocks were sectionized and stained with hematoxylin and eosin and tartrate resistance acid phosphatase (TRAP) staining (Wako, Japan).

    Article Title: Pim1 promotes the maintenance of bone homeostasis by regulating osteoclast function.
    Article Snippet: The tartrate-resistant phosphatase (TRAP) staining kit was purchased from FUJIFILM Wako Pure Chemical Corporation.

    Article Title: Xenogenous implanted dental follicle stem cells promote periodontal regeneration through inducing the N2 phenotype of neutrophils
    Article Snippet: Staining procedures, including Hematoxylin-Eosin (H&E, Solarbio, China), Masson’s trichrome (Solarbio), Tartrate-Resistant Acid Phosphatase (TRAP, Wako, Japan), immunohistochemistry (IHC), and immunofluorescence (IF), were performed following previously established methods [ ].

    Article Title: Rheumatoid arthritis severity is mediated by crosstalk between synoviocytes and mature osteoclasts through a calcium and cytokine feedback loop.
    Article Snippet: Thereafter, the fixed cells and tissues were stained with a tartrate-resistant acid phosphatase (TRAP)/alkaline phosphatase staining kit (FUJIFILM) according to the manufacturer’s instructions.

    Article Title: FOXO1-mTOR pathway in vascular pericyte regulates the formation of type H vessels to control bone metabolism
    Article Snippet: The activity of osteoclasts was detected by tartrate-resistant acid phosphatase (TRAP) staining kits (Cat. No. 294–67001, Wako, Japan) following the manufacturers’ instructions.

    Article Title: Mitochondria-targeted delivery of zinc-coordinated resveratrol nanoparticles rescues the osteogenic potential of periodontal ligament stem cells compromised by inflammation for periodontal wound healing
    Article Snippet: In addition, a tartrate-resistant acid phosphatase (TRAP) staining kit (294–67001; Wako, Japan) was used to examine bone resorption activity.

    Expressing:

    Article Title: Zymosan-Treated SKG Mice: Assessing Effects of Systemic Inflammation on the Temporomandibular Joint.
    Article Snippet: Objectives: The effects of systemic inflammation on the temporomandibular joint (TMJ) are poorly understood.. This study aimed to establish a mouse model to study the effects of systemic inflammation on the TMJ.. Materials and Methods: SKG mice, a BALB/c strain with spontaneous onset of rheumatoid arthritislike symptoms due to a spontaneous point mutation (W163C) in the gene encoding the SH2 domain of ZAP70, were treated with zymosan (β1,3glucan).

    Article Title: Therapeutic Potential of Targeting Ferroptosis in Periprosthetic Osteolysis Induced by Ultra-High-Molecular-Weight Polyethylene Wear Debris
    Article Snippet: The paraffin blocks were sectionized and stained with hematoxylin and eosin and tartrate resistance acid phosphatase (TRAP) staining (Wako, Japan).

    Article Title: Pim1 promotes the maintenance of bone homeostasis by regulating osteoclast function.
    Article Snippet: The tartrate-resistant phosphatase (TRAP) staining kit was purchased from FUJIFILM Wako Pure Chemical Corporation.

    Article Title: Xenogenous implanted dental follicle stem cells promote periodontal regeneration through inducing the N2 phenotype of neutrophils
    Article Snippet: Staining procedures, including Hematoxylin-Eosin (H&E, Solarbio, China), Masson’s trichrome (Solarbio), Tartrate-Resistant Acid Phosphatase (TRAP, Wako, Japan), immunohistochemistry (IHC), and immunofluorescence (IF), were performed following previously established methods [ ].

    Article Title: Rheumatoid arthritis severity is mediated by crosstalk between synoviocytes and mature osteoclasts through a calcium and cytokine feedback loop.
    Article Snippet: Thereafter, the fixed cells and tissues were stained with a tartrate-resistant acid phosphatase (TRAP)/alkaline phosphatase staining kit (FUJIFILM) according to the manufacturer’s instructions.

    Article Title: FOXO1-mTOR pathway in vascular pericyte regulates the formation of type H vessels to control bone metabolism
    Article Snippet: The activity of osteoclasts was detected by tartrate-resistant acid phosphatase (TRAP) staining kits (Cat. No. 294–67001, Wako, Japan) following the manufacturers’ instructions.

    Article Title: Mitochondria-targeted delivery of zinc-coordinated resveratrol nanoparticles rescues the osteogenic potential of periodontal ligament stem cells compromised by inflammation for periodontal wound healing
    Article Snippet: In addition, a tartrate-resistant acid phosphatase (TRAP) staining kit (294–67001; Wako, Japan) was used to examine bone resorption activity.

    Western Blot:

    Article Title: Zymosan-Treated SKG Mice: Assessing Effects of Systemic Inflammation on the Temporomandibular Joint.
    Article Snippet: Objectives: The effects of systemic inflammation on the temporomandibular joint (TMJ) are poorly understood.. This study aimed to establish a mouse model to study the effects of systemic inflammation on the TMJ.. Materials and Methods: SKG mice, a BALB/c strain with spontaneous onset of rheumatoid arthritislike symptoms due to a spontaneous point mutation (W163C) in the gene encoding the SH2 domain of ZAP70, were treated with zymosan (β1,3glucan).

    Article Title: Therapeutic Potential of Targeting Ferroptosis in Periprosthetic Osteolysis Induced by Ultra-High-Molecular-Weight Polyethylene Wear Debris
    Article Snippet: The paraffin blocks were sectionized and stained with hematoxylin and eosin and tartrate resistance acid phosphatase (TRAP) staining (Wako, Japan).

    Article Title: Pim1 promotes the maintenance of bone homeostasis by regulating osteoclast function.
    Article Snippet: The tartrate-resistant phosphatase (TRAP) staining kit was purchased from FUJIFILM Wako Pure Chemical Corporation.

    Article Title: Xenogenous implanted dental follicle stem cells promote periodontal regeneration through inducing the N2 phenotype of neutrophils
    Article Snippet: Staining procedures, including Hematoxylin-Eosin (H&E, Solarbio, China), Masson’s trichrome (Solarbio), Tartrate-Resistant Acid Phosphatase (TRAP, Wako, Japan), immunohistochemistry (IHC), and immunofluorescence (IF), were performed following previously established methods [ ].

    Article Title: Rheumatoid arthritis severity is mediated by crosstalk between synoviocytes and mature osteoclasts through a calcium and cytokine feedback loop.
    Article Snippet: Thereafter, the fixed cells and tissues were stained with a tartrate-resistant acid phosphatase (TRAP)/alkaline phosphatase staining kit (FUJIFILM) according to the manufacturer’s instructions.

    Article Title: FOXO1-mTOR pathway in vascular pericyte regulates the formation of type H vessels to control bone metabolism
    Article Snippet: The activity of osteoclasts was detected by tartrate-resistant acid phosphatase (TRAP) staining kits (Cat. No. 294–67001, Wako, Japan) following the manufacturers’ instructions.

    Article Title: Mitochondria-targeted delivery of zinc-coordinated resveratrol nanoparticles rescues the osteogenic potential of periodontal ligament stem cells compromised by inflammation for periodontal wound healing
    Article Snippet: In addition, a tartrate-resistant acid phosphatase (TRAP) staining kit (294–67001; Wako, Japan) was used to examine bone resorption activity.

    Tomography:

    Article Title: Zymosan-Treated SKG Mice: Assessing Effects of Systemic Inflammation on the Temporomandibular Joint.
    Article Snippet: Objectives: The effects of systemic inflammation on the temporomandibular joint (TMJ) are poorly understood.. This study aimed to establish a mouse model to study the effects of systemic inflammation on the TMJ.. Materials and Methods: SKG mice, a BALB/c strain with spontaneous onset of rheumatoid arthritislike symptoms due to a spontaneous point mutation (W163C) in the gene encoding the SH2 domain of ZAP70, were treated with zymosan (β1,3glucan).

    Article Title: Therapeutic Potential of Targeting Ferroptosis in Periprosthetic Osteolysis Induced by Ultra-High-Molecular-Weight Polyethylene Wear Debris
    Article Snippet: The paraffin blocks were sectionized and stained with hematoxylin and eosin and tartrate resistance acid phosphatase (TRAP) staining (Wako, Japan).

    Article Title: Pim1 promotes the maintenance of bone homeostasis by regulating osteoclast function.
    Article Snippet: The tartrate-resistant phosphatase (TRAP) staining kit was purchased from FUJIFILM Wako Pure Chemical Corporation.

    Article Title: Xenogenous implanted dental follicle stem cells promote periodontal regeneration through inducing the N2 phenotype of neutrophils
    Article Snippet: Staining procedures, including Hematoxylin-Eosin (H&E, Solarbio, China), Masson’s trichrome (Solarbio), Tartrate-Resistant Acid Phosphatase (TRAP, Wako, Japan), immunohistochemistry (IHC), and immunofluorescence (IF), were performed following previously established methods [ ].

    Article Title: Rheumatoid arthritis severity is mediated by crosstalk between synoviocytes and mature osteoclasts through a calcium and cytokine feedback loop.
    Article Snippet: Thereafter, the fixed cells and tissues were stained with a tartrate-resistant acid phosphatase (TRAP)/alkaline phosphatase staining kit (FUJIFILM) according to the manufacturer’s instructions.

    Article Title: FOXO1-mTOR pathway in vascular pericyte regulates the formation of type H vessels to control bone metabolism
    Article Snippet: The activity of osteoclasts was detected by tartrate-resistant acid phosphatase (TRAP) staining kits (Cat. No. 294–67001, Wako, Japan) following the manufacturers’ instructions.

    Article Title: Mitochondria-targeted delivery of zinc-coordinated resveratrol nanoparticles rescues the osteogenic potential of periodontal ligament stem cells compromised by inflammation for periodontal wound healing
    Article Snippet: In addition, a tartrate-resistant acid phosphatase (TRAP) staining kit (294–67001; Wako, Japan) was used to examine bone resorption activity.

    Micro-CT:

    Article Title: Zymosan-Treated SKG Mice: Assessing Effects of Systemic Inflammation on the Temporomandibular Joint.
    Article Snippet: Objectives: The effects of systemic inflammation on the temporomandibular joint (TMJ) are poorly understood.. This study aimed to establish a mouse model to study the effects of systemic inflammation on the TMJ.. Materials and Methods: SKG mice, a BALB/c strain with spontaneous onset of rheumatoid arthritislike symptoms due to a spontaneous point mutation (W163C) in the gene encoding the SH2 domain of ZAP70, were treated with zymosan (β1,3glucan).

    Article Title: Therapeutic Potential of Targeting Ferroptosis in Periprosthetic Osteolysis Induced by Ultra-High-Molecular-Weight Polyethylene Wear Debris
    Article Snippet: The paraffin blocks were sectionized and stained with hematoxylin and eosin and tartrate resistance acid phosphatase (TRAP) staining (Wako, Japan).

    Article Title: Pim1 promotes the maintenance of bone homeostasis by regulating osteoclast function.
    Article Snippet: The tartrate-resistant phosphatase (TRAP) staining kit was purchased from FUJIFILM Wako Pure Chemical Corporation.

    Article Title: Xenogenous implanted dental follicle stem cells promote periodontal regeneration through inducing the N2 phenotype of neutrophils
    Article Snippet: Staining procedures, including Hematoxylin-Eosin (H&E, Solarbio, China), Masson’s trichrome (Solarbio), Tartrate-Resistant Acid Phosphatase (TRAP, Wako, Japan), immunohistochemistry (IHC), and immunofluorescence (IF), were performed following previously established methods [ ].

    Article Title: Rheumatoid arthritis severity is mediated by crosstalk between synoviocytes and mature osteoclasts through a calcium and cytokine feedback loop.
    Article Snippet: Thereafter, the fixed cells and tissues were stained with a tartrate-resistant acid phosphatase (TRAP)/alkaline phosphatase staining kit (FUJIFILM) according to the manufacturer’s instructions.

    Article Title: FOXO1-mTOR pathway in vascular pericyte regulates the formation of type H vessels to control bone metabolism
    Article Snippet: The activity of osteoclasts was detected by tartrate-resistant acid phosphatase (TRAP) staining kits (Cat. No. 294–67001, Wako, Japan) following the manufacturers’ instructions.

    Article Title: Mitochondria-targeted delivery of zinc-coordinated resveratrol nanoparticles rescues the osteogenic potential of periodontal ligament stem cells compromised by inflammation for periodontal wound healing
    Article Snippet: In addition, a tartrate-resistant acid phosphatase (TRAP) staining kit (294–67001; Wako, Japan) was used to examine bone resorption activity.

    Immunohistochemical staining:

    Article Title: Zymosan-Treated SKG Mice: Assessing Effects of Systemic Inflammation on the Temporomandibular Joint.
    Article Snippet: Objectives: The effects of systemic inflammation on the temporomandibular joint (TMJ) are poorly understood.. This study aimed to establish a mouse model to study the effects of systemic inflammation on the TMJ.. Materials and Methods: SKG mice, a BALB/c strain with spontaneous onset of rheumatoid arthritislike symptoms due to a spontaneous point mutation (W163C) in the gene encoding the SH2 domain of ZAP70, were treated with zymosan (β1,3glucan).

    Article Title: Therapeutic Potential of Targeting Ferroptosis in Periprosthetic Osteolysis Induced by Ultra-High-Molecular-Weight Polyethylene Wear Debris
    Article Snippet: The paraffin blocks were sectionized and stained with hematoxylin and eosin and tartrate resistance acid phosphatase (TRAP) staining (Wako, Japan).

    Article Title: Pim1 promotes the maintenance of bone homeostasis by regulating osteoclast function.
    Article Snippet: The tartrate-resistant phosphatase (TRAP) staining kit was purchased from FUJIFILM Wako Pure Chemical Corporation.

    Article Title: Xenogenous implanted dental follicle stem cells promote periodontal regeneration through inducing the N2 phenotype of neutrophils
    Article Snippet: Staining procedures, including Hematoxylin-Eosin (H&E, Solarbio, China), Masson’s trichrome (Solarbio), Tartrate-Resistant Acid Phosphatase (TRAP, Wako, Japan), immunohistochemistry (IHC), and immunofluorescence (IF), were performed following previously established methods [ ].

    Article Title: Rheumatoid arthritis severity is mediated by crosstalk between synoviocytes and mature osteoclasts through a calcium and cytokine feedback loop.
    Article Snippet: Thereafter, the fixed cells and tissues were stained with a tartrate-resistant acid phosphatase (TRAP)/alkaline phosphatase staining kit (FUJIFILM) according to the manufacturer’s instructions.

    Article Title: FOXO1-mTOR pathway in vascular pericyte regulates the formation of type H vessels to control bone metabolism
    Article Snippet: The activity of osteoclasts was detected by tartrate-resistant acid phosphatase (TRAP) staining kits (Cat. No. 294–67001, Wako, Japan) following the manufacturers’ instructions.

    Article Title: Mitochondria-targeted delivery of zinc-coordinated resveratrol nanoparticles rescues the osteogenic potential of periodontal ligament stem cells compromised by inflammation for periodontal wound healing
    Article Snippet: In addition, a tartrate-resistant acid phosphatase (TRAP) staining kit (294–67001; Wako, Japan) was used to examine bone resorption activity.

    Marker:

    Article Title: Zymosan-Treated SKG Mice: Assessing Effects of Systemic Inflammation on the Temporomandibular Joint.
    Article Snippet: Objectives: The effects of systemic inflammation on the temporomandibular joint (TMJ) are poorly understood.. This study aimed to establish a mouse model to study the effects of systemic inflammation on the TMJ.. Materials and Methods: SKG mice, a BALB/c strain with spontaneous onset of rheumatoid arthritislike symptoms due to a spontaneous point mutation (W163C) in the gene encoding the SH2 domain of ZAP70, were treated with zymosan (β1,3glucan).

    Article Title: Therapeutic Potential of Targeting Ferroptosis in Periprosthetic Osteolysis Induced by Ultra-High-Molecular-Weight Polyethylene Wear Debris
    Article Snippet: The paraffin blocks were sectionized and stained with hematoxylin and eosin and tartrate resistance acid phosphatase (TRAP) staining (Wako, Japan).

    Article Title: Pim1 promotes the maintenance of bone homeostasis by regulating osteoclast function.
    Article Snippet: The tartrate-resistant phosphatase (TRAP) staining kit was purchased from FUJIFILM Wako Pure Chemical Corporation.

    Article Title: Xenogenous implanted dental follicle stem cells promote periodontal regeneration through inducing the N2 phenotype of neutrophils
    Article Snippet: Staining procedures, including Hematoxylin-Eosin (H&E, Solarbio, China), Masson’s trichrome (Solarbio), Tartrate-Resistant Acid Phosphatase (TRAP, Wako, Japan), immunohistochemistry (IHC), and immunofluorescence (IF), were performed following previously established methods [ ].

    Article Title: Rheumatoid arthritis severity is mediated by crosstalk between synoviocytes and mature osteoclasts through a calcium and cytokine feedback loop.
    Article Snippet: Thereafter, the fixed cells and tissues were stained with a tartrate-resistant acid phosphatase (TRAP)/alkaline phosphatase staining kit (FUJIFILM) according to the manufacturer’s instructions.

    Article Title: FOXO1-mTOR pathway in vascular pericyte regulates the formation of type H vessels to control bone metabolism
    Article Snippet: The activity of osteoclasts was detected by tartrate-resistant acid phosphatase (TRAP) staining kits (Cat. No. 294–67001, Wako, Japan) following the manufacturers’ instructions.

    Article Title: Mitochondria-targeted delivery of zinc-coordinated resveratrol nanoparticles rescues the osteogenic potential of periodontal ligament stem cells compromised by inflammation for periodontal wound healing
    Article Snippet: In addition, a tartrate-resistant acid phosphatase (TRAP) staining kit (294–67001; Wako, Japan) was used to examine bone resorption activity.

    Immunohistochemistry:

    Article Title: Zymosan-Treated SKG Mice: Assessing Effects of Systemic Inflammation on the Temporomandibular Joint.
    Article Snippet: Objectives: The effects of systemic inflammation on the temporomandibular joint (TMJ) are poorly understood.. This study aimed to establish a mouse model to study the effects of systemic inflammation on the TMJ.. Materials and Methods: SKG mice, a BALB/c strain with spontaneous onset of rheumatoid arthritislike symptoms due to a spontaneous point mutation (W163C) in the gene encoding the SH2 domain of ZAP70, were treated with zymosan (β1,3glucan).

    Article Title: Therapeutic Potential of Targeting Ferroptosis in Periprosthetic Osteolysis Induced by Ultra-High-Molecular-Weight Polyethylene Wear Debris
    Article Snippet: The paraffin blocks were sectionized and stained with hematoxylin and eosin and tartrate resistance acid phosphatase (TRAP) staining (Wako, Japan).

    Article Title: Pim1 promotes the maintenance of bone homeostasis by regulating osteoclast function.
    Article Snippet: The tartrate-resistant phosphatase (TRAP) staining kit was purchased from FUJIFILM Wako Pure Chemical Corporation.

    Article Title: Xenogenous implanted dental follicle stem cells promote periodontal regeneration through inducing the N2 phenotype of neutrophils
    Article Snippet: Staining procedures, including Hematoxylin-Eosin (H&E, Solarbio, China), Masson’s trichrome (Solarbio), Tartrate-Resistant Acid Phosphatase (TRAP, Wako, Japan), immunohistochemistry (IHC), and immunofluorescence (IF), were performed following previously established methods [ ].

    Article Title: Rheumatoid arthritis severity is mediated by crosstalk between synoviocytes and mature osteoclasts through a calcium and cytokine feedback loop.
    Article Snippet: Thereafter, the fixed cells and tissues were stained with a tartrate-resistant acid phosphatase (TRAP)/alkaline phosphatase staining kit (FUJIFILM) according to the manufacturer’s instructions.

    Article Title: FOXO1-mTOR pathway in vascular pericyte regulates the formation of type H vessels to control bone metabolism
    Article Snippet: The activity of osteoclasts was detected by tartrate-resistant acid phosphatase (TRAP) staining kits (Cat. No. 294–67001, Wako, Japan) following the manufacturers’ instructions.

    Article Title: Mitochondria-targeted delivery of zinc-coordinated resveratrol nanoparticles rescues the osteogenic potential of periodontal ligament stem cells compromised by inflammation for periodontal wound healing
    Article Snippet: In addition, a tartrate-resistant acid phosphatase (TRAP) staining kit (294–67001; Wako, Japan) was used to examine bone resorption activity.

    Immunofluorescence:

    Article Title: Zymosan-Treated SKG Mice: Assessing Effects of Systemic Inflammation on the Temporomandibular Joint.
    Article Snippet: Objectives: The effects of systemic inflammation on the temporomandibular joint (TMJ) are poorly understood.. This study aimed to establish a mouse model to study the effects of systemic inflammation on the TMJ.. Materials and Methods: SKG mice, a BALB/c strain with spontaneous onset of rheumatoid arthritislike symptoms due to a spontaneous point mutation (W163C) in the gene encoding the SH2 domain of ZAP70, were treated with zymosan (β1,3glucan).

    Article Title: Therapeutic Potential of Targeting Ferroptosis in Periprosthetic Osteolysis Induced by Ultra-High-Molecular-Weight Polyethylene Wear Debris
    Article Snippet: The paraffin blocks were sectionized and stained with hematoxylin and eosin and tartrate resistance acid phosphatase (TRAP) staining (Wako, Japan).

    Article Title: Pim1 promotes the maintenance of bone homeostasis by regulating osteoclast function.
    Article Snippet: The tartrate-resistant phosphatase (TRAP) staining kit was purchased from FUJIFILM Wako Pure Chemical Corporation.

    Article Title: Xenogenous implanted dental follicle stem cells promote periodontal regeneration through inducing the N2 phenotype of neutrophils
    Article Snippet: Staining procedures, including Hematoxylin-Eosin (H&E, Solarbio, China), Masson’s trichrome (Solarbio), Tartrate-Resistant Acid Phosphatase (TRAP, Wako, Japan), immunohistochemistry (IHC), and immunofluorescence (IF), were performed following previously established methods [ ].

    Article Title: Rheumatoid arthritis severity is mediated by crosstalk between synoviocytes and mature osteoclasts through a calcium and cytokine feedback loop.
    Article Snippet: Thereafter, the fixed cells and tissues were stained with a tartrate-resistant acid phosphatase (TRAP)/alkaline phosphatase staining kit (FUJIFILM) according to the manufacturer’s instructions.

    Article Title: FOXO1-mTOR pathway in vascular pericyte regulates the formation of type H vessels to control bone metabolism
    Article Snippet: The activity of osteoclasts was detected by tartrate-resistant acid phosphatase (TRAP) staining kits (Cat. No. 294–67001, Wako, Japan) following the manufacturers’ instructions.

    Article Title: Mitochondria-targeted delivery of zinc-coordinated resveratrol nanoparticles rescues the osteogenic potential of periodontal ligament stem cells compromised by inflammation for periodontal wound healing
    Article Snippet: In addition, a tartrate-resistant acid phosphatase (TRAP) staining kit (294–67001; Wako, Japan) was used to examine bone resorption activity.



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    Identification of mature osteoclasts (mOC) on bone slices and quantification of resorbing activity 21 days after onset of the differentiation process. A triple staining for OC markers was applied (TRAP, red; F-actin, green; nuclei: blue; (A) . A cell was classified as mOC when it TRAP-positive and had ≥ 3 nuclei (white arrowheads; all mOC showed an actin ring, (B) , precursor (preOC, round with one or two nuclei, yellow arrowheads, (C) , proOC (spindle-shaped with a single nucleus, red arrowhead, (D) . After microscopy, cells were removed, and resorption pits were visualized by Toluidine blue staining (E) . Semi-quantitative image analysis was performed using ImageJ and masks to measure the resorbed areas per field of view (F) . Scale bar: 50 µm (A-D) , 100 µm (E, F) .

    Journal: Frontiers in Immunology

    Article Title: Modulation of Differentiation and Bone Resorbing Activity of Human (Pre-) Osteoclasts After X-Ray Exposure

    doi: 10.3389/fimmu.2022.817281

    Figure Lengend Snippet: Identification of mature osteoclasts (mOC) on bone slices and quantification of resorbing activity 21 days after onset of the differentiation process. A triple staining for OC markers was applied (TRAP, red; F-actin, green; nuclei: blue; (A) . A cell was classified as mOC when it TRAP-positive and had ≥ 3 nuclei (white arrowheads; all mOC showed an actin ring, (B) , precursor (preOC, round with one or two nuclei, yellow arrowheads, (C) , proOC (spindle-shaped with a single nucleus, red arrowhead, (D) . After microscopy, cells were removed, and resorption pits were visualized by Toluidine blue staining (E) . Semi-quantitative image analysis was performed using ImageJ and masks to measure the resorbed areas per field of view (F) . Scale bar: 50 µm (A-D) , 100 µm (E, F) .

    Article Snippet: The staining solution consists of TRAP buffer (0.1 M acetate buffer: 35.2 ml 0.2 M Sodium Acetate solution, 14.8 ml 0.2 M acetic acid, 50 ml; Millipore, Burlington, USA) mixed with 0.3 M Sodium Tartrate (pH 5.5), 10 mg/ml Naphthol AS-MX phosphate, Triton X-100, 0.3 mg/ml Fast Red Violet LB Salt (all from Sigma Aldrich Chemie GmbH).

    Techniques: Activity Assay, Staining, Microscopy