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transcriptor reverse transcriptase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher transcriptor reverse transcriptase
    Transcriptor Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcriptor+reverse+transcriptases/transcriptor+reverse+transcriptase/pm40037500-63-9-12
    Average 90 stars, based on 1 article reviews
    transcriptor reverse transcriptase - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Nonclinical evaluation of a Vitis vinifera extract towards a novel antiaging cosmetic ingredient.
    Article Snippet: 3- (4,5- dimethylthiazol- 2- yl)- 2,5- diphenyltetrazolium bromide (MTT), H2O2, DMSO, PBS solution, TriPure Isolation Reagent®, Methanol, Acetic acid, DPPH solution, Cytocrom C, Xanthine, Ethanol, and octyl- methoxycinnamate were purchased from Sigma- Aldrich. .. 2’,7’- Dichlorofluorescin Diace (DCFH- Da) kit, EBSS solution, TRIzol®, COX- 2 kit assay, oligo (dT), Transcriptor Reverse Transcriptase, HeLa cells were purchased from Thermo Fischer®. .. MMP- 12 kit assay was purchased from Abcam.

    Article Title: RNAi-Mediated Knockdown of Calreticulin3a Impairs Pollen Tube Growth in Petunia
    Article Snippet: 3′RACE was carried out using the 5′/3′ RACE Kit (Merck, Darmstadt, Germany) according to the manufacturer’s protocol. .. Reverse transcription was performed with the Transcriptor Reverse Transcriptase and Oligo dT-Anchor Primer in the presence of RiboLock RNase Inhibitor (Thermo Fisher Scientific, Waltham, MA, USA) at 55 °C for 1 h. A 1 μL aliquot of the reverse transcription reaction was then used in the outer 3′-RACE PCR with the PCR anchor primer and 3′-RACE gene-specific outer primer , along with PlatinumTM SuperFiTM DNA Polymerase. ..

    Article Title: R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein
    Article Snippet: RNA integrity was assessed with an Agilent 2100 Bioanalyzer (Agilent Technologies). .. Reverse transcription was performed on 2.2 μg total RNA with Transcriptor reverse transcriptase, random hexamers, dNTPs and ten units of RNAse OUT (Thermo Scientific) following the manufacturer's protocol in a total volume of 20 μl. .. End-point PCR reactions were done on 10 ng cDNA in 10 μl final volume containing 0.2 mmol l −1 each dNTP, 1.5 mmol l −1 MgCl 2 , 0.6 μmol l −1 each primer, and 0.2 units of Platinum Taq DNA polymerase (Thermo Scientific).

    Article Title: Rapid screening of primary and rationally synthesized anti-mycobacterial compounds in macrophage using double recombinant M. bovis BCG strain.
    Article Snippet: Antimycobacterial screening is done primarily at three levels; in vitro, ex vivo and in vivo.. In earlier studies, we had generated a double recombinant Mycobacterium bovis BCG strain carrying firefly and Renilla luciferase genes as two reporters under the control of a constitutive and an inducible mycobacterial promoter.. The presence of dual reporters allows simultaneous expression and analysis of two reporter enzymes within a single system.

    Article Title: Identification of Novel Prognostic Biomarkers That are Associated with Immune Microenvironment Based on GABA-Related Molecular Subtypes in Gastric Cancer
    Article Snippet: .. DNA was prepared using Transcriptor reverse transcriptase (ThermoFisher Scientific, USA). .. The gene expression level was quantified by SYBR green PCR Master Mix (ThermoFisher Scientific, USA) using a Real-Time PCR System (ThermoFisher Scientific, USA).

    Article Title: Overexpression of eis without a mutation in promoter region of amikacin- and kanamycin-resistant Mycobacterium tuberculosis clinical strain
    Article Snippet: The cDNA synthesis was performed using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, USA). .. RNA was reverse-transcribed in 20 μl reaction mixture containing Transcriptor reverse transcriptase buffer (50 mM Tris–HCl (pH 8.3), 50 mM KCl, 4 mM MgCl 2 and 10 mM DTT), 10 U of protector RNase Inhibitor (Promega, USA), 0.25 mM each dNTP (Invitrogen, USA), 2 μM RT primer (Additional file : Table S1), 1 μg of RNA and 10 U of Transcriptor reverse transcriptase (Thermo Fisher Scientific, USA). ..

    Article Title: A chronic pro-inflammatory environment contributes to the physiopathology of actinic lentigines
    Article Snippet: .. Complementary DNA (cDNA) was synthetized by reverse transcription of total RNA in presence of oligo(dT) and Transcriptor Reverse Transcriptase (Invitrogen, Thermo Fisher Scientific, USA). cDNA was then amplified using SsoAdvanced PreAmp Supermix (BIORAD). .. Q-PCR was performed using the LightCycler system (Roche Molecular System Inc., USA) and SYBR Green reagent mix (Ozyme, France) according to suppliers’ instructions.

    Polymerase Chain Reaction:

    Article Title: RNAi-Mediated Knockdown of Calreticulin3a Impairs Pollen Tube Growth in Petunia
    Article Snippet: 3′RACE was carried out using the 5′/3′ RACE Kit (Merck, Darmstadt, Germany) according to the manufacturer’s protocol. .. Reverse transcription was performed with the Transcriptor Reverse Transcriptase and Oligo dT-Anchor Primer in the presence of RiboLock RNase Inhibitor (Thermo Fisher Scientific, Waltham, MA, USA) at 55 °C for 1 h. A 1 μL aliquot of the reverse transcription reaction was then used in the outer 3′-RACE PCR with the PCR anchor primer and 3′-RACE gene-specific outer primer , along with PlatinumTM SuperFiTM DNA Polymerase. ..

    Synthesized:

    Article Title: Rapid screening of primary and rationally synthesized anti-mycobacterial compounds in macrophage using double recombinant M. bovis BCG strain.
    Article Snippet: Antimycobacterial screening is done primarily at three levels; in vitro, ex vivo and in vivo.. In earlier studies, we had generated a double recombinant Mycobacterium bovis BCG strain carrying firefly and Renilla luciferase genes as two reporters under the control of a constitutive and an inducible mycobacterial promoter.. The presence of dual reporters allows simultaneous expression and analysis of two reporter enzymes within a single system.

    Amplification:

    Article Title: A chronic pro-inflammatory environment contributes to the physiopathology of actinic lentigines
    Article Snippet: .. Complementary DNA (cDNA) was synthetized by reverse transcription of total RNA in presence of oligo(dT) and Transcriptor Reverse Transcriptase (Invitrogen, Thermo Fisher Scientific, USA). cDNA was then amplified using SsoAdvanced PreAmp Supermix (BIORAD). .. Q-PCR was performed using the LightCycler system (Roche Molecular System Inc., USA) and SYBR Green reagent mix (Ozyme, France) according to suppliers’ instructions.



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