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transcriptor reverse transcriptase  (Millipore)


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    Structured Review

    Millipore transcriptor reverse transcriptase
    Transcriptor Reverse Transcriptase, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcriptor+reverse+transcriptases/transcriptor+reverse+transcriptase/pm39930703-141-27-30
    Average 90 stars, based on 1 article reviews
    transcriptor reverse transcriptase - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: The (p)ppGpp-binding GTPase Era promotes rRNA processing and cold shock survival in Staphylococcus aureus
    Article Snippet: .. Complementary DNA was synthesised from 1.5 μg RNA with transcriptor reverse transcriptase (Sigma) and random primers. .. RT-PCR was performed on 100 ng of cDNA in triplicate using SYBR Green Jumpstart Taq readymix (Sigma).

    Article Title: Long-Term Memory in Drosophila Is Influenced by Histone Deacetylase HDAC4 Interacting with SUMO-Conjugating Enzyme Ubc9
    Article Snippet: Flies were snap frozen and heads collected over dry ice, and then total RNA was extracted from ∼50 heads with TRIzol and quantified on a NanoDrop. .. Complementary DNA (cDNA) was synthesized with random primers (Sigma-Aldrich, St. Louis, MO) and Transcriptor Reverse Transcriptase (Sigma-Aldrich). ..

    Article Title: Elevated Blood Hemoglobin in Different Cavefish Populations Evolves Through Diverse Hemoglobin Gene Expression Patterns.
    Article Snippet: We constructed cDNA libraries for each population by annealing with Oligo‐dT primers (Invitrogen) at 65°C for 10min using a BioRad C100 thermocycler. .. Then, we added 4 μL (5x) Transcriptor RT reaction buffer (Millipore Sigma), 0.5 μL Protector RNase Inhibitor (Millipore‐Sigma), 2 μL dNTP mix (10mM; Roche), and 0.5 μL Transcriptor Reverse Transcriptase (Millipore‐Sigma), and reactions were incubated at 50°C for 60min, followed by inactivation of RT enzyme at 85°C for 5min. cDNA preps were then stored at −20°C until use. ..

    Synthesized:

    Article Title: Long-Term Memory in Drosophila Is Influenced by Histone Deacetylase HDAC4 Interacting with SUMO-Conjugating Enzyme Ubc9
    Article Snippet: Flies were snap frozen and heads collected over dry ice, and then total RNA was extracted from ∼50 heads with TRIzol and quantified on a NanoDrop. .. Complementary DNA (cDNA) was synthesized with random primers (Sigma-Aldrich, St. Louis, MO) and Transcriptor Reverse Transcriptase (Sigma-Aldrich). ..

    Incubation:

    Article Title: Elevated Blood Hemoglobin in Different Cavefish Populations Evolves Through Diverse Hemoglobin Gene Expression Patterns.
    Article Snippet: We constructed cDNA libraries for each population by annealing with Oligo‐dT primers (Invitrogen) at 65°C for 10min using a BioRad C100 thermocycler. .. Then, we added 4 μL (5x) Transcriptor RT reaction buffer (Millipore Sigma), 0.5 μL Protector RNase Inhibitor (Millipore‐Sigma), 2 μL dNTP mix (10mM; Roche), and 0.5 μL Transcriptor Reverse Transcriptase (Millipore‐Sigma), and reactions were incubated at 50°C for 60min, followed by inactivation of RT enzyme at 85°C for 5min. cDNA preps were then stored at −20°C until use. ..



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