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Cellscript Inc scriptcap 2'-o-methyltransferase kit
Scriptcap 2' O Methyltransferase Kit, supplied by Cellscript Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scriptcap+kit/scriptcap+2++o+methyltransferase+kit/ppr0953852-108-12-15
Average 90 stars, based on 1 article reviews
scriptcap 2'-o-methyltransferase kit - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Methods for in vitro evolution of constructs derived from viruses
Article Snippet: Resulting replicon RNAs were capped and methylated using the ScriptCapTM m7G Capping System and ScriptCapTM 2′-O-Methyltransferase Kit (Cellscript) according to the manufacturer's instructions.

Article Title: Calibrated ribosome profiling assesses the dynamics of ribosomal flux on transcripts.
Article Snippet: The RNAs were then capped and polyadenylated using a ScriptCap m7G capping system (CELLSCRIPT), a ScriptCap 2′-O-Methyltransferase Kit (CELLSCRIPT), and an A-Plus poly(A) polymerase tailing kit (CELLSCRIPT).

Article Title: Ionizable lipidoids and their uses
Article Snippet: For all other experiments, replicon RNAs were synthesized essentially the same way, except for inclusion of 2′-O-methyltransferase (from ScriptCap 2′-O-methyltransferase kits; CellScript, according to the manufacturer's protocol) in the capping step to methylate the cap-adjacent 5′ nucleotide of the RNA, thus producing a cap-1 structure and ensuring more efficient first-round endogenous translation.

Article Title: Sense codon-misassociated eRF1 elicits widespread ribosome stalling and induction of quality control
Article Snippet: Capping and polyadenylation were conducted using a ScriptCap m 7 G Capping Kit (CELLSCRIPT), a ScriptCap 2′- O -Methyltransferase Kit (CELLSCRIPT), and an A-Plus Poly (A) Polymerase Tailing Kit (CELLSCRIPT).

Article Title: Making and using in vitro-synthesized ssRNA for introducing into mammalian cells to induce a biological or biochemical effect
Article Snippet: In some embodiments, said post-transcriptional capping of the treated ssRNAs or the purified ssRNAs is performed as described above and/or in the product literature provided with the SCRIPTCAPTM m7G Capping System, the SCRIPTCAPTM 2′-O-methyltransferase kit, or the 17 mSCRIPTTM standard mRNA production system with respect to the capping enzyme system components (all from CELLSCRIPT, Inc., Madison, WI, USA).

Article Title: Position-Specific Nucleoside Sugar Modifications in mRNA ORF: Enhancing Translational Function through Complete Chemical Synthesis of mRNA
Article Snippet: Puri ed RNA was capped with a Vaccinia Capping System (NEB) and ScriptCap 2'-O-Methyltransferase Kit (CELLSCRIPT), according to the manufacturer’s protocol.

Article Title: Making and using in vitro-synthesized ssRNA for introducing into mammalian cells to induce a biological or biochemical effect
Article Snippet: In some of these embodiments, the one or more in vitro-synthesized ssRNA molecules that exhibit the 5′ cap were synthesized prior to said treating and/or purifying: (i) co-transcriptionally by incorporation of a cap analog (e.g., an anti-reverse cap analog or ARCA, or e.g., a thio-ARCA)) during in vitro transcription (e.g., using the MESSAGEMAX T7 ARCA-capped message transcription kit or the INCOGNITO T7 ARCA 5mC- and Ψ-RNA transcription kit, CELLSCRIPT, INC., Madison, WI, USA); or (ii) post-transcriptionally by incubating in vitro-transcribed ssRNA molecules with a capping enzyme system comprising RNA guanyltransferase under conditions wherein the in vitro-transcribed ssRNA molecules are 5′-capped, including wherein the capping enzyme system results in methylation of the 2′ hydroxyl of the ribose in the 5′ penultimate nucleotide (e.g., using T7 mSCRIPT standard mRNA production system, or using a separate in vitro transcription system, such as the T7-SCRIBE standard RNA IVT kit, the INCOGNITO T7 Ψ-RNA transcription kit, or the INCOGNITO T7 5mC- and Ψ-RNA transcription kit to obtain ssRNA, and the SCRIPTCAP m7G capping system to obtain cap0 RNA (all from CELLSCRIPT, INC.); in some preferred embodiments, the capping enzyme system further results in methylation of the 2′ hydroxyl of the ribose in the 5′ penultimate nucleotide to generate cap1 RNA, and another step for synthesizing said in vitro-synthesized ssRNA or mRNA comprises: incubating the in vitro-transcribed ssRNA or mRNA with RNA 2′-O-methyltransferase (e.g., using the SCRIPTCAP 2′-O-methyltransferase kit, CELLSCRIPT, INC.).

Article Title: Calibrated ribosome profiling assesses the dynamics of ribosomal flux on transcripts
Article Snippet: The RNAs were then capped and polyadenylated using a ScriptCap m 7 G capping system (CELLSCRIPT), a ScriptCap 2′- O -Methyltransferase Kit (CELLSCRIPT), and an A-Plus poly(A) polymerase tailing kit (CELLSCRIPT).



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